Lipids: Chapter 10 (Part III)
Lipids: Chapter 10 (Part III)
Lipids: Chapter 10 (Part III)
• Ubiquinone (also called coenzyme Q) and plastoquinone are isoprenoids that function as lipophilic
electron carriers in the oxidation-reduction reactions that drive ATP synthesis in mitochondria and
chloroplasts, respectively. Both ubiquinone and plastoquinone can accept either one or two
electrons and either one or two protons
• Dolichols Activate Sugar Precursors for Biosynthesis. During assembly of the complex
carbohydrates of bacterial cell walls, and during the addition of polysaccharide
Lipids
Summary
• Some types of lipids, although present in relatively small quantities, play critical roles as cofactors or
signals.
• Prostaglandins, thromboxanes, and leukotrienes (the eicosanoids), derived from arachidonate, are
extremely potent hormones.
• Steroid hormones, derived from sterols, serve as powerful biological signals, such as the sex
hormones.
Lipids
Summary
• Vitamins D, A, E, and K are fat-soluble compounds made up of isoprene units. All play essential
roles in the metabolism or physiology of animals. Vitamin D is precursor to a hormone that regulates
calcium metabolism. Vitamin A furnishes the visual pigment of the vertebrate eye and is a regulator
of gene expression during epithelial cell growth. Vitamin E functions in the protection of membrane
lipids from oxidative damage, and vitamin K is essential in the blood-clotting process
• Dolichols activate and anchor sugars on cellular membranes for use in the synthesis of certain
complex carbohydrates, glycolipids, and glycoproteins
Lipids
Fatty acid catabolism
• The properties that make triacylglycerols good storage compounds, however, present problems in
their role as fuels. Because they are insoluble in water, ingested triacylglycerols must be emulsified
before they can be digested by water-soluble enzymes in the intestine, and triacylglycerols
absorbed in the intestine or mobilized from storage tissues must be carried in the blood bound to
proteins that counteract their insolubility.
• the chemical steps of fatty acid oxidation in mitochondria. The complete oxidation of fatty acids to
CO2 and H2O takes place in three stages:
1. The oxidation of long-chain fatty acids to two-carbon fragments, in the form of acetyl-CoA (
oxidation);
2. The oxidation of acetyl-CoA to CO2 in the citric acid cycle
3. The transfer of electrons from reduced electron carriers to the mitochondrial respiratory chain
Lipids
Digestion, Mobilization, and Transport of Fats
• Triacylglycerols provide more than half the energy requirements of some organs, particularly the
liver, heart, and resting skeletal muscle.
• Stored triacylglycerols are virtually the sole source of energy in hibernating animals and migrating
birds
Lipids
Dietary Fats Are Absorbed in the Small Intestine
• In vertebrates, before ingested triacylglycerols can be absorbed through the intestinal wall they
must be converted from insoluble macroscopic fat particles to finely dispersed microscopic micelles
• This solubilization is carried out by bile salts (amphipathic), such as taurocholic acid which are
synthesized from cholesterol in the liver, stored in the gallbladder, and released into the small
intestine after ingestion of a fatty mea
• Micelle formation enormously increases the fraction of lipid molecules accessible to the action of
water-soluble lipases in the intestine, and lipase action converts triacylglycerols to
monoacylglycerols (monoglycerides) and diacylglycerols (diglycerides), free fatty acids, and
glycerol
• These products of lipase action diffuse into the epithelial cells lining the intestinal surface (the
intestinal mucosa)
Lipids
Dietary Fats Are Absorbed in the Small Intestine
• where they are reconverted to triacylglycerols and packaged with dietary cholesterol and specific
proteins into lipoprotein aggregates called chylomicrons
The surface is a layer of phospholipids, with head groups facing the aqueous phase Triacylglycerols sequestered in the interior
(yellow) make up more than 80% of the mass. Several apolipoproteins that protrude from the surface (B-48, C-III, C-II) act as
signals in the uptake and metabolism of chylomicron contents. The diameter of chylomicrons ranges from about 100 to 500 nm
Lipids
Dietary Fats Are Absorbed in the Small Intestine
• Apolipoproteins are lipid-binding proteins in the blood, responsible for the transport of
triacylglycerols, phospholipids, cholesterol, and cholesteryl esters between organs. Apolipoproteins
(“apo” means “detached” or “separate,” designating the protein in its lipid-free form) combine with
lipids to form several classes of lipoprotein particles, spherical aggregates with hydrophobic lipids at
the core and hydrophilic protein side chains and lipid head groups at the surface. Various
combinations of lipid and protein produce particles of different densities, ranging from chylomicrons
and very low- density lipoproteins (VLDL) to very-high-density lipoproteins (VHDL), which can be
separated by ultracentrifugation.
• The protein moieties of lipoproteins are recognized by receptors on cell surfaces. In lipid uptake
from the intestine, chylomicrons, which contain apolipoprotein C-II (apoC-II), move from the
intestinal mucosa into the lymphatic system, and then enter the blood, which carries them to muscle
and adipose tissue
Lipids
Dietary Fats Are Absorbed in the Small Intestine
• In the capillaries of these tissues, the extracellular enzyme lipoprotein lipase, activated by apoC-II,
hydrolyzes triacylglycerols to fatty acids and glycerol which are taken up by cells in the target
tissues
• In muscle, the fatty acids are oxidized for energy; in adipose tissue, they are re-esterified for
storage as triacylglycerols
• The remnants of chylomicrons, depleted of most of their triacylglycerols but still containing
cholesterol and apolipoproteins, travel in the blood to the liver, where they are taken up by
endocytosis, mediated by receptors for their apolipoproteins
• Triacylglycerols that enter the liver by this route may be oxidized to provide energy or to provide
precursors for the synthesis of ketone bodies
Lipids
Dietary Fats Are Absorbed in the Small Intestine
• When the diet contains more fatty acids than are needed immediately for fuel or as precursors, the
liver converts them to triacylglycerols, which are packaged with specific apolipoproteins into VLDLs.
The VLDLs are transported in the blood to adipose tissues, where the triacylglycerols are removed
and stored in lipid droplets within adipocytes.
Lipids
Assignment
• IP3