(c) What is the molar concentration of the complex

in a solution that has the absorbance described

Applied Chemistry 4 – Tutorial 3
in (a) whenAbsorptionmeasured at 470 nm in a 2.50-cm
Spectroscopy
(1) cell?
24-24. The complex formed between Cu(I) and 1,10-
phenanthroline has a molar absorptivity of 7000 L
cm21 mol21 at 435 nm, the wavelength of maximum
absorption. Calculate
(a) the absorbance of a 6.17 3 1025 M solution of
the complex when measured in a 1.00-cm cell at
24-19. A solution 435 nm.containing 4.48 ppm KMnO4 exhibits 24
85.9the
(b) %T in a 1.00-cm
percent transmittancecell at 520
of thenm. Calculate
solution the
in (a).
molar
(c) theabsorptivity
concentration of KMnO 4 at this
of a solution wavelength.
that in a 5.00-cm
24-20. Beryllium(II) cell has the forms a complexaswith
same absorbance acetylacetone
the solution in (a).
(166.2
(d) the pathg/mol).
length Calculate
through athe 3.13molar
3 1025absorptivity
M solution 24
of theof the complex,
complexgiven that a 2.25
that is needed for an ppm solution
absorbance that
has isa thetransmittance
same as the solution of 37.5% when measured in a
in (a).
*24-25. A 1.00-cm
solutioncell withata 295“true”nm, the wavelength
absorbance of maxi-
[A 5 2log(P 0/P)]
mum
(2)
of 2.10absorption.
was placed in a spectrophotometer with a
*24-21. stray At 580 nm,percentage
light the wavelength (Ps/P of) of
its 0.75.
maximumWhat absorp-
absor-
210
tion, the
bance Ar complex
would beFe(SCN)
measured? has
Whata molar absorptivity
percentage error
3
of 7.00result?
would 3 10 L cm mol . Calculate
21 21

(a) the absorbance of a 3.40 3 10 –5 M solution 24

ofcontent
Unless otherwise noted, all the oncomplex
this page is ©atCengage
580Learning.
nm in a 1.00-cm cell.
(b) the absorbance of a solution in which the con-
centration of the complex is twice that in (a).
(c) the transmittance of the solutions described in
(a) and (b).
(d) the absorbance of a solution that has half the
transmittance of that described in (a).
24-22. A 2.50-mL aliquot of a solution that contains
4.33 ppm iron(III) is treated with an appropriate
excess of KSCN and diluted to 50.0 mL. What is
 of the
and itscomplex
standardthat is neededfrom
deviation for an
theabsorbance that
least-squares
(3) equation
is the same ofasthetheline. Compare
solution in (a).the unknown con-
*24-25. A solution centration withtoa that“true”obtained
absorbance manually from a 0cali-
[A 5 2log(P /P)]
bration
of 2.10 wascurve.placed in a spectrophotometer with a
(b)
strayWhat lightmass in grams
percentage (Psof/P0phosphorus
) of 0.75. What was elimi-
absor-
bance nated per daybebymeasured?
Ar would the patient? What percentage error
(c)
would What is the phosphate concentration in urine in
result?
(4) mM?
Unless otherwise noted, all content on this page is © Cengage Learning.
24-28. Nitrite is commonly determined by a colorimetric
procedure using a reaction called the Griess reac-
682!!CHAPTER tion. In this 24 reaction, the sample Introduction to Spectroche
containing nitrite
682!!CHAPTER is reacted24with sulfanilimide and N-(1-Napthyl)
Introduction to Spectrochemic
ethylenediamine to form a colored species that ab-
sorbs at 550 nm.
instrument, the Using
following an automated
results were flowobtained
analysis for
instrument,
standard solutions the following results
of nitrite andwere
for obtained
a samplefor con-
standard
taining ansolutions unknown of nitrite
amount: and for a sample con-
taining an unknown amount:
Solution Absorbance at 550 nm
Solution Absorbance at 550 nm
2.00 µM 0.065
2.00
6.00 µM
µM 0.065
0.205
6.00 µM 0.205
10.00 µM 0.338
10.00 µM 0.338
14.00 µM 0.474
14.00 µM 0.474
18.00 µM
18.00 µM 0.598
0.598
Unknown
Unknown 0.402
0.402
(a) Find
(a) Find the
theslope,
slope,intercept,
intercept,andandstandard
standard deviation
deviation
of the
of thecalibration
calibrationcurve.
curve.
(b) Construct
(b) Constructthe thecalibration
calibrationcurve.
curve.
(c) Determine
(c) Determinethe theconcentration
concentrationofofnitrite
nitrite
in in
thethe
sampleand
sample anditsitsstandard
standarddeviation.
deviation.
24-29. The
24-29. The equilibrium
equilibriumconstant
constantfor
forthe
thereaction
reaction
22 2222
2CrO 2H18
12H 8CrCr
2OO HH
2O O
1
2CrO4 221 7 11