Previous Year Question Paper

of

Examination

(Original Question Paper with Answer Key)

GRADUATE APTITUDE TEST IN ENGINEERING

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GATE 2016 BIOCHEMISTRY

XL-I : BIOCHEMISTRY
Q. 1 – Q. 10 carry one mark each.
Q.1 Heterologous expression of green fluorescent protein is possible because the genetic code is

m
(A) universal (B) triplet (C) degenerate (D) non-overlapping
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Q.2 Phosphoglucose isomerase was incubated with 0.2 M of glucose 6-phosphate. On reaching

co
rium
equilibrium, 55% of glucose 6-phosphate was converted to fructose 6-phosphate. The equilibrium
constant for this reaction is _______.
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Q.3 Hydrolysis of a peptide involves cleavage of the bond between the atoms

s.
(A) N and C (B) C and O (C) C and C (D) N and C
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Q.4 Inter-conversion of UDP-glucose and UDP-galactose is catalyzed by

as
(A) an oxidase (B) a kinase (C) an epimerase (D) a mutase
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Q.5 Gel filtration profile and corresponding activity data for a pure enzyme
yme are shown iin the figure

cl
below. The same enzyme sample on SDS-PAGE runs as a 30 kDa polypeptide.

Absorbancee

gy 0.4
Enzyme
me activity
vity

40
Absorbance (280 nm)

Enzyme activity (U)

lo
0.3 30

0.2 20
Absorba

io

0.1 10

0 0
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60 kDa 30 kDa
Elution volume (ml)
as

Which
ch onee of the following is the correct interpretation of the data?
(A)
A) Bothh monomer and dim
dimer are active (B) Enzyme is active only as a monomer
(C)
C) Protein does not form
fo dimers (D) Enzyme is active only as a dimer
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Q.6 Amino acid resid

residues predominantly involved in protein-DNA interactions are
(A)
A) alanine
aalaniness (B) negatively charged
(C) prolines
prolin (D) positively charged
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Q.7 Cel
Cellulose serves as a structural polymer whereas starch does not. This is because cellulose contains
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(A) linked glucose monomers and inter-chain hydrogen bonds

(B) linked glucose monomers and intra-chain hydrogen bonds
(C) linked glucose monomers and inter-chain hydrogen bonds
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(D) linked glucose monomers and intra-chain hydrogen bonds

"OT
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Q.8 Molar absorption spectra labeled (i), (ii) and (iii) for three different amino acids are shown below.

Molar absorbance,  × 10-3

m
(i)

co
3

2 (ii)

s.
1

(iii)
0

as
240 260 280 300
Wave length (nm)

Which one of the following is the correct combination of spectral

ral assignments?
signments?

cl
(A) (i) - tryptophan, (ii) - tyrosine, (iii) - phenylalanine
(B) (i) - phenylalanine, (ii) - tryptophan, (iii) - tyrosine
(C) (i) - proline, (ii) - tyrosine, (iii) - tryptophan

Q.9
gy The fluidity of a phospholipid membrane increases
nine
(D) (i) - tryptophan, (ii) - proline, (iii) - phenylalanine

creases when the fatty acid

(A) chain length increases and degree off unsaturation decreases
aci
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(B) chain length decreases and degreee of unsaturation increase
increases
(C) chain length decreases and degree
gree of unsaturation ddecre
decreases
(D) chain length increases and degree of unsaturat
unsaturation inc
increases "OT
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Q.10 hesized on the riboso

Polypeptides are biosynthesized ribosomes iinside the cell. Chemical synthesis of
polypeptides is also possible
ssible Merrifield’s solid-phase peptide synthesis. In both the cases
sible through Merrifield
Me
the polypeptide chain
in is extended one amino
am acid at a time. The direction of polypeptide synthesis
yb

is from
(A) C-terminusus to NN-terminus
-term
terminus
inu in both the cases
(B) N-terminus
minuss to C-terminus in bo
both the cases
terminuss to N-terminus oon the ribosomes and N-terminus to C-terminus in solid-phase
(C) C-terminus
as

thesiss
synthesis
D) N-terminus
(D) erminus to C-termi
C
C-terminus on the ribosomes and C-terminus to N-terminus in solid-phase
nthesis
synthesis
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%
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Q. 11 – Q. 220 carr
carry two marks each.
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Q.11 Four groups of metabolites are given below. Choose the group in which all the compounds contain
aat least one bond whose Go of hydrolysis is ≤ −7.0kcal/mole.
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(A) Glucose 1-phosphate, Adenosine triphosphate, Fructose 1,6-bisphosphate

(B) Creatine phosphate, Acetyl phosphate, Succinyl CoA
(C) Glycerol 3-phosphate, Acetyl CoA, 1,3-Bisphosphoglycerate
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(D) Glucose 6-phosphate, Phosphoenolpyruvate, Adenosine diphosphate

"OT
#

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Q.12 The Go for the malate dehydrogenase catalyzed step of Krebs cycle is +7.1 kcal/mole.
Nevertheless, the conversion of malate to oxaloacetate in vivo proceeds spontaneously because the
subsequent reaction that consumes oxaloacetate has a Go of
(A) −3.0 kcal/mole

m
(B) +3.0 kcal/mole
(C) −7.7 kcal/mole
(D) +7.7 kcal/mole "OT
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Q.13 When freshly isolated intact mitochondria were incubated with ADP and inorganic phosphatee
neither the oxygen consumption nor the ATP synthesis could be detected. Addition of succinate
cinate
uent addition
resulted in increased oxygen consumption as well as ATP synthesis with time. Subsequent
of cyanide to this system will result in which one of the following?

s.
(A) Both oxygen consumption and ATP synthesis are inhibited
(B) Oxygen consumption continues but ATP synthesis is inhibited
(C) Oxygen consumption is inhibited but ATP synthesis continues

as
(D) Both oxygen consumption and ATP synthesis continue
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Q.14 Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enz
enzyme and
subjected to agarose gel electrophoresis. Five DNA fragments of different
ifferent sizes were
we observed
ob and

cl
es of DNA ends gen
their sizes summed up to 4200 bp. The number of picomoles generated after complete
digestion with the enzyme is _______.

Q.15
gy (Given: average molecular weight of each base pairr is 660

An enzyme was purified using ion-exchange

below.
60 Da)

ge chromatography
romatography and th
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the results are shown in the table

lo
Step Volumeme (ml) Total protein ((mg) Total activity (U)
Cell extract 80000 400 800
DEAE Sephacel 10 2 200
io

Which one of the following

ng is thee correct inter
interpreta
interpretation of these data?
on was achieved wi
(A) 50 fold purification with 25% yield of the enzyme
yb

cation was achieved with 50%

(B) 25 fold purification 5 yield of the enzyme
(C) 50 fold purification
ification
ion was achieved
achieve with
wit 4% yield of the enzyme
(D) 200 fold purification
cation was achieved with 25% yield of the enzyme "OT
"
Q.16 Aspartate dues are found in the active sites of many enzymes. The pKa for the -carboxylate of
tate residues
as

aspartate
artatee is 3.86. At phy
physiological pH this group can function as
physiolo
a a conjugate
(A) a nucleophile and c acid (B) an electrophile and a conjugate acid
C) a nucleophile
(C) nucleophil and a conjugate base (D) an electrophile and a conjugate base
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Q.17
17 Kinetic
netic parameters
parame
pa for the enzyme fumarase with three different substrates are given below.

Substrate M)
KM ( kcat (sec-1)
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Fluorofumarate 27 2700
Fumarate 5 800
Chlorofumarate 111 20
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The specificity of fumarase for the substrates decreases in the order

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(A) Fluorofumarate > Fumarate > Chlorofumarate

(B) Chlorofumarate > Fluorofumarate > Fumarate
(C) Fumarate > Fluorofumarate > Chlorofumarate
(D) Fumarate > Chlorofumarate > Fluorofumarate
"OT
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Q.18 A polypeptide with the amino acid sequence ‘AGKPDHEKAHL’ was dissolved in a buffer of pH
1.8. The predominant form of the polypeptide will have a net charge of
(A) +4 (B) +5 (C) +7 (D) +11
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Q.19 An N-terminal His-tagged protein of molecular weight 40 kDa was purified using Ni-NTA column.
This protein sample was subjected to SDS-PAGE. A western blot of the same using anti-His
antibodies is shown below.

co
60 kDa

s.
40 kDa

30 kDa

as
20 kDa

10 kDa

cl
gyWhich one of the following interpretations is correct?
(A) Only the His-tag of the protein got removed
(B) The protein forms oligomers
oved
rrect?

(C) The purified protein sample is homogeneous

mogeneous
geneous
lo
(D) The protein has a stable N-terminal
minal 20 kDa domain "OT
%

Q.20 de that forms a transmembrane

The sequence of a polypeptide transmemb
transm helix is shown below.
io

1 11
1 21 31 41
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TGERVQLAHH
HH FSEPEITLII
SEPEITL FGVMAGVIGT ILLASYGIRR LIKKSP

Which onee of the

he following segmen
se
segments of the peptide is most likely to span the membrane?
as

(A) E3-G22
3-G222 (B) V5
V5-A25 (C) E15-A34 (D) F21-R40

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END OF THE QUESTION PAPER

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