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    Wright Stain.1420 PDF

    Uploaded by

    Yasmine Montt

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    of 1

    2505 Parview Road ● Middleton, WI 53562-2579 ● 800-383-7799 ● www.newcomersupply.com ● newly@newcomersupply.

    com

    Part 1420
    Revised October 2015

    Wright Stain for Smears - Technical Memo


    SOLUTION: 500 ml 1 Liter 1 Gallon
    Wright Stain Part 1420A Part 1420B Part 1420C

    Additionally Needed:
    Alcohol, Methanol Anhydrous, ACS Part 12236
    Wright Stain Buffer, pH 6.8 Part 1430

    For storage requirements and expiration date refer to individual bottle labels.

    APPLICATION: PROCEDURE NOTES:

    Newcomer Supply Wright Stain for Smears, provides an unbuffered 1. The timings provided in this procedure are suggested ranges.
    Wright staining solution used for differential staining of cell types in Optimal staining times will depend upon staining intensity
    peripheral blood smears as well as bone marrow smears/films. preference.
    2. Smears containing primarily normal cell populations require
    METHOD: minimum staining time; immature cells may require a longer
    staining time. Bone marrow smears/films may also require a longer
    Technique: Flat staining rack method staining time.
    Solutions: All solutions are manufactured by Newcomer Supply, Inc. 3. The color range of the stained cells may vary depending upon the
    pH of the buffer and the pH of the rinse water used.
    STAINING PROCEDURE: a. Alkalinity is indicated by red blood cells being blue-grey
    and white blood cells only blue.
    1. Prepare within an accepted time frame, a well-made blood smear
    b. Acidity is indicated by red blood cells being bright red or
    or bone marrow smear/film per your laboratories protocol, with a
    pink and lack of proper staining in white blood cells.
    focus on uniform cell distribution.
    c. If necessary adjust buffer pH accordingly to 6.8 +/ - 0.2.
    2. Allow slides to thoroughly air-dry prior to staining.
    3. Filter Wright Stain Solution prior to use with high quality filter paper.
    REFERENCES:
    4. Place slides on flat staining rack suspended over sink.
    5. Fix smears by flooding slides with Methanol (12236) for 10-30
    1. Lillie, R. D., and Harold Fullmer. Histopathologic Technic and
    seconds.
    Practical Histochemistry. 4th ed. New York: McGraw-Hill, 1976.
    6. Shake off excess Methanol; flood each slide with 1 ml of filtered
    747-748.
    Wright Stain for 3-5 minutes.
    2. McPherson, Richard and Matthew Pincus. Henry’s Clinical
    a. See Procedure Notes #1 and #2.
    Diagnosis and Management by Laboratory Methods. 22nd ed.
    7. Retain Wright Stain on slides. Directly add 1 ml of Wright Stain
    Philadelphia: Elsevier Saunders, 2011. 522-532.
    Buffer, pH 6.8 to each slide; gently agitate to completely mix with
    3. Sheehan, Dezna C., and Barbara B. Hrapchak. Theory and
    retained Wright Stain.
    Practice of Histotechnology. 2nd ed. St. Louis: Mosby, 1980. 154-
    8. Stain for an additional 6-10 minutes.
    155.
    9. Wash well in distilled water; rinse thoroughly in running tap water.
    4. Modifications developed by Newcomer Supply Laboratory.
    10. Air-dry slides in a vertical position; examine microscopically.
    11. If coverslip is preferred, allow slides to air-dry and coverslip with
    compatible mounting medium.

    RESULTS:

    Erythrocytes Pink
    Neutrophils Granules - Purple
    Eosinophils Granules - Pink
    White blood cells Chromatin - Purple
    Lymphocytes Cytoplasm - Blue
    Monocytes Cytoplasm - Blue
    Bacteria Deep Blue

    SUPPORT/WARRANTY: For assistance regarding this product contact Newcomer Supply at 800-383-7799 or newly@newcomersupply.com. The information presented in this
    technical memo is to the best of our knowledge accurate. No warranty is expressed or implied. The user is responsible for determining the suitability of this product for their
    use and upon receipt assumes all liability for its use and responsibility for compliance with any laws or regulations. Please refer to www.newcomersupply.com for complete
    warranty information. © Newcomer Supply, Inc. Active September 2014 Page 1 of 1

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