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  • Thermo Scientific Richard-Allan Scientific Wright Stain Solution Instructions For Use

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    Thermo Scientific Richard-Allan Scientific Wright Stain Solution Instructions For Use

    Uploaded by

    Kifayat Hussain
    D22

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    of 1

    Thermo Scientific Richard-Allan Scientific Wright Stain Solution

    Instructions for Use

    Specimen Collection Results


    Fresh blood film or fresh EDTA anticoagulated blood film and bone marrow films. Specimens should be air Erythrocytes – Pale Pink
    dried after smear has been prepared and fixed in absolute methanol for 15 seconds. Eosinophilic Granules – Reddish Orange
    Leukocyte Nuclei – Purple
    Mode of Action Cytoplasm – Bluish Purple
    Neutrophilic Granules – Light Purple
    The Thermo Scientific™ Richard-Allan Scientific Wright Stain Solution is a mixture of several thiazin dyes in a
    methanol solvent. Ionic and nonionic forces are involved in the binding of these dyes. The stain solution has Discussion
    anionic and cationic properties. The negatively charged phosphoric acid groups of DNA attract the purple poly-
    chromatic cationic dyes to the nuclei. The blue basophilic granules are stained by the polychromatic cationic Wright Stain Solution should be stored at room temperature. This staining reagent is for “In Vitro” use only. Refer
    dyes. Cationic cellular components, such as erythrocytes and eosinophilic granules, are stained by the red and to the Material Safety Data Sheet for Health and Safety Information. All reagents are stable and should not form
    pink anionic dyes. The buffers used in the staining procedure liberate and activate dye ions allowing them to precipitants under ordinary storage parameters. It is recommended that the Wright Stain Solution be discarded
    chemically bond with specific cellular components. When staining blood and bone marrow smears, the pH of after each use. All dyes used in this formulation have been certified by the Biological Stain Commission.
    the staining solution and/or buffer is a critical factor.
    Technical Comments
    Technical Procedure
    Thicker films and bone marrow preparations will require longer staining times. Because there is variation in the
    Immersion Staining Protocol pH of tap water, use of tap water in procedure may hinder staining results. Distilled or deionized water should be
    1. Air dry smears. used during the staining procedure and preparation of phosphate buffer. The “ripening” of the polychromed dye
    is a continuous chemical reaction. Therefore, the stock solution should not be used or diluted after the expiration
    2. Fix smears in absolute methanol for 15 seconds to 5 minutes. date. The staining procedure may require modification to suit personal preference. Blood films which have not
    3. Stain smears in Wright Stain Solution for 2 minutes. been thoroughly air dried before staining may show sloughing of cells from slide.
    4. Stain smears in mixture of 50ml Wright Stain Solution and 75ml of Phosphate Buffer pH 6.6 solution
    (Catalog #89032) and 175ml of distilled or deionized water for 5 minutes. References
    5. Rinse smears in standing deionized water for 1.5 minutes, or rinse briefly in running deionized water. 1. Raphael, S.S. Lynch’s Medical Laboratory Technology, Fourth Edition. Saunders Company, Philadelphia, PA,
    6. Air dry smears. 1983.
    7. Examine smears under a microscope. 2. Lillie, R.D. H.J. Conn’s Biological Stains, Ninth Edition. Williams and Watkins Company, Baltimore, MD, 1977.
    3. Brown, B.A. Hematology: Principles and Procedures, Fourth Edition. Lea and Febiger Company, Philadelphia,
    Horizontal Staining Protocol
    PA, 1984.
    1. Place slide with thoroughly dried film on a horizontal staining rack.
    2. Flood smear with absolute methanol for 15-30 seconds and then drain. Ordering Information
    3. Flood smear with Wright Stain Solution for 3 minutes.
    Product Size Qty. REF
    4. Add equal amount of Phosphate Buffer pH 6.6 solution to smear and let stand for 45 seconds.
    Wright Stain Solution 1 gal 1 89010
    5. Rinse smear thoroughly with deionized water.
    Wright Stain Solution 2.5 gal 1 89011
    6. Air dry and examine under a microscope. Wright Stain Solution 32 oz 1 89012
    Phosphate Buffer pH 6.6 (powder) 9.25g 12/cs 89032

    ©2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

    Anatomical Pathology Richard-Allan Scientific Thermo Shandon Limited


    Subsidiary of Thermo Fisher Scientific Subsidiary of Thermo Fisher Scientific
    4481 Campus Drive, Kalamazoo, MI 49008 Tudor Road, Manor Park,
    Phone: 1 (800) 522-7270 Runcorn, Cheshire WA7 1TA, UK
    Phone: + 1 (269) 544-5600 Phone: +44 (0) 1928 534 050
    IS89012 R7/13 www.thermoscientific.com/pathology Fax: + 44 (0) 1928 534 049

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