Full Water Book EN

Smart H2O for you,
and your science
Thermo Scientific
Waterbook
Smart H2O for you,
and your science
Pure water is elemental to the success of your
experiments. But finding the right water system for
your research goes deeper than water quality alone.
You need a smart choice that supports both your
science and your budget – not only on day 1, but with
every cartridge and filter change. One that reflects
130 years of innovations, like feed water monitoring,
hands-free dispensing and effortless cartridge
change-outs. With a Thermo Scientific™ Barnstead™
lab water system, the only thing you’ll have in your
water is confidence.
The following are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries:
B-Pure, Bantam, Barnstead, E-Pure, GenPure, Harvey, H2O Select, LabTower, MagaPure,
MicroPure, Pacific, Pura-Lite, Smart2Pure, and Ventgard.
Contents
Lab Water 101
n Introduction 2
Thermo Scientific Barnstead Water Purification Technologies 6
Distillation, Filtration and Ultrafiltration, Reverse Osmosis
Deionization, Electrodeionization, Adsorption, Ultraviolet Oxidation
International Water Standards 14
Advanced Technologies Used to Monitor Purity 15
Conductivity and Resistivity
Build Your Own Water System 16
What Type of Water Do You Need?
Ultrapure (Type 1) Water
Pure (Type 2) Water
Reverse Osmosis (RO) Water
Cartridge and Filter Systems
Lab Case Studies
Thermo Scientific Water Purification Products

n Thermo Scientific Type 1 Ultrapure Water Purification Systems 22
Barnstead GenPure xCAD Plus 26
Barnstead GenPure Pro 30
Barnstead GenPure 34
Barnstead MicroPure 38
Barnstead E-Pure 42
Barnstead LabTower EDI 44
Barnstead Smart2Pure 48
Barnstead Smart2Pure Pro 52
n Thermo Scientific Type 2 Pure Water Purification Systems 56
Barnstead Pacific TII 60
Barnstead LabTower TII 64
Barnstead Mega-Pure Glass Stills 68
Barnstead Classic Stills 72
Barnstead Classic Still Storage Reservoirs and Accessories 74
Barnstead Distillation 101 76
n Thermo Scientific Reverse Osmosis Water Purification Systems 78
Barnstead LabTower RO 84
Barnstead Pacific RO 86
n Pretreatment 90
n Thermo Scientific Cartridge and Filter Systems 96
Barnstead B-Pure 100
Barnstead Bantam Deionizer 104
Barnstead Hose Nipple Cartridges 105
Barnstead B-Pure 1/2 Size Filters 106
n Legacy Water System Consumables 108
Type 1 systems 108
Type 2/3 systems 109
n Support and Maintenance 110
Contact the Lab Water Experts 110
Services 111
Go Green 112
Common Conversions 113
n Glossary 114
thermofisher.com/purewater 1
Lab Water Experts
For more than 130 years, Thermo Scientific lab water
systems have been a trusted resource for science and
industry. Our complete line of water purification technologies
includes solutions for your most critical and everyday
application needs, from electrodeionization to reverse
osmosis and distillation.
Barnstead lab water systems deliver:
Innovation
Our water purification portfolio features advanced ergonomics and
technology, including remote dispensing and UV intensity monitoring –
that offer users full control with confidence.
Flexibility
Laboratory environments often have limited bench space. Our portfolio
offers numerous mounting options, small footprints and flexible
dispensing options to provide you the luxury of designing a configuration
that best suits your lab.
Convenience
Our Thermo Scientific H20 Select analysis program and complete
all-in-one box ordering gives you the confidence to select the right
solution. Receive a complete water system using one part number
and have the components you need to easily maintain your new water
purification system.
2
Innovation Flexibility Convenience
that deliver accuracy system design with customized
With innovative water monitoring Mounting flexibility system selection
technology, Thermo Scientific water Installation options include under the Based on the results of a H2O Select
purification systems eliminate expense cabinet, on the wall, on the bench, analysis, we will recommend the best
and lost time which may occur with or free-standing units with wheels for water system for your application
contaminated lab water. added mobility. based on your feed water quality,
Advanced feed water monitoring Full system control at the laboratory applications, daily volume
Alerts the user to fluctuations in the Thermo Scientific xCAD requirements and budget. We do all
feed water quality. Poor feed water will remote dispenser the thinking for you.
reduce cartridge life. Eliminate the hassle of going How it works:
TOC measurements with UV back to the primary system to
1. Contact your local sales
intensity monitoring adjust parameters.
representative for a complimentary
Continuously monitoring and Adjustable storage tank H2O Select analysis kit.
recording TOC measurements in Growing the lab or planning for
2. Fill the sample bottle with your
real-time, this technology ensures changing needs? Type 2 and RO
anticipated feed water.
ultrapure water that meets or exceeds systems enable changing water level
even the most stringent requirements settings in the storage tank to adapt to 3. Answer the short questionnaire
for sensitive applications. A photo the lab’s needs. pertaining to your water require-
sensor continually checks the intensity ments, applications and budget.
Dual water quality
of the UV lamp. A drop in measured Need more than one type of water? 4. Place the completed application
UV radiation can result in an incorrect Many of the systems produce two and water sample bottle in the
TOC measurement. types of water from just one system. return box. Add postage and
EDI systems feature tank mail to the Thermo Scientific
recirculation to maintain high Barnstead Water Lab (return label
purity water even during long included in box).
periods of inactivity All-in-one-box ordering
Automatic recirculation of the water Have confidence in knowing that there
in the tank across a special polisher are no surprises with added post sale
actively protects against bacteria costs. Everything you need ships
growth even during long periods under one part number!
of inactivity. Easy self maintenance
The Aquastop quick connections
make changing your cartridges quick
and simple.
Lab
Water
101
Common Water Impurities

Don’t let impurities contaminate
your sensitive experiments.
Thermo Scientific Barnstead
water purification systems are
engineered to remove impurities
that threaten your research so you
can focus on what’s important –
your next discovery.
Suspended Particles Colloids Inorganic Ions Dissolved Organics

Sand, silt, clay and other Colloidal particles typically Impurities such as silicates, Organic solids are
suspended particles cause have a slightly net negative chlorides, fluorides, present from plant and
water to be turbid. These charge, range in size from bicarbonates, sulfates, animal decay and from
suspended particles can 0.01 – 1.0 µm, and can be phosphates, nitrates human activity. They may
interfere with instrument either organic or inorganic. and ferrous compounds include proteins, alcohols,
operation, plug valves and Unlike suspended are present as cations chloramines and residues
other narrow flow paths, particles, colloids do (positively charge ions) and of pesticides, herbicides
and foul reverse osmosis not settle out by gravity, anions (negatively charged and detergents. They
membranes. They typically but remain suspended ions). Water with a high foul ion exchange resins,
range from 1 –10 µm in the liquid that carries concentration of ions will interfere with organic
in size. them. Colloids clog filters, conduct electricity readily analyses including HPLC,
interfere with instrument and have high conductivity gas chromatography and
operation, foul reverse and low resistivity, as fluoroscopy. They will also
osmosis membranes and conductivity and resistivity hinder electrophoresis,
can bypass ion exchange are inversely related. Ions tissue and cell culture.
resins, resulting in lower will adversely affect the
resistivity in deionized results of inorganic analyses
water systems. such as IC, AA, ICP/MS
and may retard cell and
tissue growth in biological
research. They can also
affect the cartridge life in
deionized water systems.
4
Speaking the Language of Lab Water
Contaminants to Avoid in Your Lab Water

Application and Interest Areas Particulates Colloids Ions Dissolved gases Organics Nucleases Pyrogens
General Lab Purpose
Autoclave • • •
Humidification • • •
Glassware washing/rinsing • • •
Media preparation • • •
Analytical
Ion chromatography (IC) • • • •
Atomic absorption (AA) • • • •
High performance liquid
• • • • •
chromatography (HPLC)
Inductively coupled plasma
• • • • •
spectroscopy (ICP)
Mass spectroscopy (MS) • • • • •
Gas chromatography (GC) • • • • •
Total organic carbon (TOC) • • • • •
Life Sciences
Genomics (ex. PCR, mutagenesis) • • • • • • •
Proteomics (ex. crystallography,
• • • • • • •
electrophoresis)
Immunology (ex. monoclonal
• • • • • • •
antibody production, blots)
Pharmacology • • • • • • •
Cell and tissue culture • • • • • • •
Drug discovery • • • • • • •
Dissolved Gases Microorganisms Pyrogens and Viruses Nucleases

Water naturally contains Bacteria, fungi and algae Pyrogens or bacterial RNase and DNase
dissolved gases such as are found in all natural endotoxins are are naturally occurring
carbon dioxide, nitrogen water sources. Chlorination lipopolysaccharide enzymes that are
and oxygen. Carbon eliminates harmful bacteria, molecules incorporated instrumental in regulating
in the cell membrane of
dioxide dissolves in water but tap water still contains bodily functions. As
gram negative bacteria.
to form weakly acidic live microorganisms Viruses are considered to important as these
carbonic acid (H2CO3), which interfere with sterile be non-living nucleic acids. enzymes are to the life
which can alter the pH applications, such as cell Both can adversely affect process, they can be
of the water. Additionally, and tissue culture. laboratory experiments devastating to nucleic acid
oxygen, the most common often hindering cell and experiments. If these
non-ionized gas, may tissue growth in culture. contaminants are present
cause corrosion of metal in the lab water used,
surfaces. the ability to amplify
DNA molecules will be
severely limited. Likewise,
experiments utilizing RNA
can be ruined.
Lab Thermo Scientific Barnstead
Water Water Purification Technologies
101
To make pure and ultrapure water, the impurities discussed on the previous page must be efficiently and
effectively removed. Barnstead water purification systems employ multiple technologies, some synergistically,
to remove impurities and give you consistently PURE water.
Water purification is a step-by-step process often requiring a combination of technologies, each of which varies in the
ability to remove specific contaminants.
The table below illustrates which impurities are removed by each technology.
Ultraviolet
Reverse Electro- Filtration Ultrafiltration Combination
Distillation Deioization Adsorption (UV)
Osmosis deionization (UF) UV/UF
Oxidation
Inorganic ions
Dissolved gasses
Organics
Particles
Bacteria
Pyrogens
Nucleases
Excellent
Good
Poor
6
Thermometer
Receiver Condenser
Distillate Water
Heat Source
A basic distillation diagram
Distillation
Distillation has the broadest removal capabilities of
any single form of water purification.
Water is boiled and undergoes phase changes during the
distillation process, changing from liquid to vapor and back
to liquid. It is the change from liquid to vapor that separates
the water (in various degrees) from many dissolved
impurities, such as ions, organic contaminants with low
boiling points <100 °C, bacteria, pyrogens and particulates.
Distillation cannot be used on its own to remove inorganic
ions, ionized gases, organics with boiling points higher than
100 °C, or dissolved non-ionized gases.
Benefits
• Offers the broadest removal capabilities of any single
form of water purification
• Requires no consumables
Limitations
• Requires periodic maintenance and manual cleaning of
system to maintain water purity
• Requires water for cooling
Barnstead Classic Stills
Systems that utilize this technology
• Barnstead Classic Stills and Mega-Pure Stills
101
Filtration Limitations
Barnstead water purification products offer both depth • Clogging

(nominal) and membrane (absolute) filters. • Will not remove organics, nucleases, pyrogens,
Depth filters are most commonly used as a pretreatment dissolved gases or dissolved inorganics
and are manufactured by winding fibers around hollow and Systems that utilize this technology
slotted tubes. As water passes through the wound fiber • Thermo Scientific Barnstead GenPure, MicroPure,
matrix toward the center tube particles are retained on the E-Pure, LabTower EDI, Smart2Pure, Pacific TII,
fibers. Traditionally this type of filter removes most of the LabTower TII water purification systems
impurities above the rated pore size of the filter. Most often
these filters are rated to remove larger particles (>1 µm) to
Sterile Filter
protect the technologies that follow.
Membrane filters are often termed absolute, meaning that
they are designed to remove all particles above the rated
pore size of the filter. These filters use a membrane,
(in flat sheet or hollow fiber form), and are most often used
at the end of a system to remove bacteria or other particles
that are not removed by the preceding technologies.
Traditionally membrane filters in laboratory water systems
have a rated pore size below 0.45 µm, most often 0.2 µm.
Benefits
• Efficient operation Ultrafilter
• Maintenance is change out only
Ultrafiltration (UF) Benefits
In water purification, ultrafiltration is used to remove • Effectively removes molecules (pyrogens, nucleases,
pyrogens (bacterial endotoxins) and nucleases, microorganisms, particulates) above their rated size
which is critical for tissue culture, cell culture and • Long life
media preparation. • Helps to remove pyrogens and nucleases
Ultrafilters use size exclusion to remove particles and Limitations
macromolecules. By design, ultrafilters operate similar to
• Will not remove dissolved inorganics, dissolved gases,
reverse osmosis membranes; particles are captured on
and organics
the surface of the membranes and are flushed from the
membrane via a reject stream. Ultrafilters are used at the end Systems that utilize this technology
of systems ensuring the near total removal of macromolecular • Barnstead GenPure, MicroPure, and Smart2Pure
impurities like pyrogens, nucleases and particulates. water purification systems
8
Reverse Osmosis
Reverse osmosis is the most economical method of
removing up to 99 % of feed water contaminants.
To understand reverse osmosis you must first understand
osmosis. During natural osmosis, water flows from a less
concentrated solution through a semipermeable membrane
to a more concentrated solution until concentration and
pressure on both sides of the membrane are equal. Pure
Water
In water purification systems, external pressure is Saline
Solution
applied to the more concentrated (feed water) side of the
membrane to reverse the natural osmotic flow. This forces
the feed water through the semipermeable membrane. Semipermeable Membrane To Drain
The impurities are deposited on the membrane surface
and sent to drain and the water that passes through the Process of reverse osmosis
membrane as product water is, for the most part, free
of impurities. Limitations
A reverse osmosis membrane has a thin microporous • Limited flow rates through the membrane require
surface that rejects impurities, but allows water to pass intermediate storage devices to meet user demand
through. The membrane rejects bacteria, pyrogens and
• Does not remove dissolved gases
90-95 % of inorganic solids. Polyvalent ions are rejected
easier than monovalent ions. Organic solids with a • Requires pretreatment to avoid damaging the membrane
molecular weight greater than 200 Daltons are rejected > Oxidation – Chlorine
by the membrane but dissolved gases are not as > Scaling – CaCO3
effectively removed. > Fouling – Organics and Colloids
> Piercing – Hard particles
Reverse osmosis is a percent rejection technology.
The purity of the product water depends on the purity of Systems that utilize this technology
the feed water. The product is typically 95-99 % higher in • Barnstead Smart2Pure,
purity than that of the feed water. LabTower EDI, Pacific TII,
Due to the restrictive nature of the membrane, the flow LabTower TII, Pacific RO,
rate is much slower than other purification technologies. LabTower RO water
This slow flow rate means that all RO systems require a purification systems
storage reservoir to provide a constant supply of RO water
ready when you need it.
Benefits
• To varying degrees, removes most types of
contaminants, bacteria, pyrogens and 90-95 % of
inorganic ions
• Requires minimal maintenance
Barnstead Pacific RO
and Barnstead Lab
Tower RO
water systems
Lab Technologies
Water
101
Deionization Limitations
Deionization is also referred to as demineralization or • Finite capacity – once all ion binding sites are occupied,
ion exchange. ions are no longer retained and the cartridge must
be replaced
The process removes ions from feed water with the use
of synthetic resins. These resins are chemically altered to • Does not remove organics, particles, pyrogens
have an affinity for dissolved inorganic ions and are divided or bacteria
into two classifications: cation removal resins and anion Systems that utilize this technology
removal resins. • Barnstead GenPure, MicroPure, E-Pure, LabTower EDI,
Cations have a positive charge and include sodium (Na ), + Smart2Pure, Pacific TII, LabTower TII, and the Bantam
calcium (Ca+2) and magnesium (Mg+2). Anions have a water purification systems, Hose Nipple, and B-Pure
negative charge and include chloride (Cl-), sulfates cartridges
(SO4-2), and bicarbonates (HCO-3). The ions are removed
from the water through a series of chemical reactions.
These reactions take place as the water passes through NaCI
the ion exchange resin beds. Cation resin contains

Na+ CI-
hydrogen (H+) ions on the surface which are exchanged
H+ OH-
for positively charged ions. Anion resin contains H+ Na+ CI- OH-
hydroxide (OH-) ions on its exchange sites which are
H+ OH-
exchanged for negatively charged ions. The final product Cation H+ OH- Anion
of these two exchanges is H+ and OH-, which combine to H+
Resin OH-
Resin OH-
form water (H2O).
H+ H+ OH- OH-
Deionization is the only technology which produces the H+ HOH OH-
resistivity requirement for Type 1 reagent grade water.

(H2O) Water
In laboratory water systems, cation and anion resins
are most often mixed together allowing them to achieve Diagram showing how cations and anions are exchanged
maximum ionic purity. on the resin
Two-bed deionization – The cation and anion resin are

NaCI
in separate halves of a cartridge. In general, this method
is less effective deionizing
Na+ water
CI- as compared to the
mixed-bed deionization;
H+
however, it isOH
more
- tolerant of
+ + OH-
other types
H of impurities.
Na CI -
Mixed-bed
H+ deionization We use semi-conductor
H+ –OH-
OH-
Cation Anion
grade mixed bed deionization resin to achieve maximum
H+
Resin -
OHthe
Resin OH-
resistivity and low TOC. Mixing cation and anion resin
drives the
H+ deionization
H+ to completion,
OH- makingOH-it more
H+ HOH OH-
efficient and more effective at the removal of ions.
Benefits (H2O) Water Inlet Two Bed Mixed-bed Water Inlet
• Removes dissolved inorganics ions very effectively

Two bed resin is shown on the left and mixed bed resin in
• Produces product water with a resistivity above on the right
18 MΩ.cm
10
Process of electrodeionization
Electrodeionization (EDI) retained by the ion exchanger resins and so continually

regenerate the resins. The salt ions migrate through the
In contrast to conventional ion exchange in which
appropriate ion selective membranes into the concentrate
resins are exhausted and must either be thrown away
chambers and are flushed out of them by water. As all
or chemically regenerated, EDI utilizes an electric
concentration chambers are flushed through one after the
current for continual resin regeneration.
other, excess H+ and OH– ions can again combine to
How EDI works form H20.
Several layers of ion selective membranes are positioned Benefits
between an anode and a cathode. Layered ion exchange
• Effectively removes ions
resin beds and concentrate chambers are alternately
positioned between them. • Continuously regenerates automatically
On applying an electric voltage, water (H20) is split into H+ Limitations

and OH– in the cell. The H+ and Na+ cations can migrate • Limited capacity – feed water must be of high quality
through the cation permeable membranes, anions through • Does not remove organics, particles, pyrogens
the anion permeable membranes. or bacteria
The ions migrate in the direction of the applied voltage, i.e. Systems that utilize this technology
anions to the positive pole (anode), cations to the negative
• Barnstead LabTower EDI water purification system
pole (cathode). The water ions H+ and OH– that migrate
through an ion exchange chamber displace salt ions
101
Adsorption
Adsorption uses high surface area activated carbon
to remove organics and chlorine from feed water.
It is used as a first or second step in most water
purification systems and may be used as a final step,
in combination with ion exchange resins, to achieve ultra
low Total Organic Carbon (TOC). Organics and chlorine
adhere to the surface of the activated carbon and remain
attached to the carbon.
Mixed-bed deionization and adsorption – We use Selection of high quality resins used in our cartridges
a combination semiconductor grade mixed-bed
deionization resins and synthetic carbon in a single
cartridge to achieve maximum resistivity and low
Total Organic Carbon (TOC). Chlorinated H2O Carbon H2O Free of Chlorine
and Some Organics
Benefits
CI2
• Removes dissolved organics and chlorine CI2
• Long life CI2

CI2 CI2
CI2
Limitations
(Chlorine and Organics)
• Will not remove ions and particulates

Feed water with organic and chlorine contaminants come
• Barnstead GenPure, MicroPure, E-Pure, LabTower EDI, into contact with the activated carbon in the cartridge.
Smart2Pure, Pacific TII, LabTower TII, and the The impurities adhere to the surface of the carbon,
allowing purified water to be produced.
Bantam water purification systems, Hose Nipple,
and B-Pure cartridges
12
Ultraviolet (UV) Oxidation Combination Ultraviolet Oxidation

Photochemical oxidation with ultraviolet light and Ultrafiltration (UV/UF)
eliminates trace organics and inactivates The use of ultraviolet oxidation and ultrafiltration
microorganisms in feed water. technologies in conjunction with adsorption and
The UV lamps in our lab water systems generate light at deionization in the same system produces water
two wavelengths, 185 and 254 nm. The light generated virtually free of all impurities. These technologies have
at 254 nm has the greatest anti-bacterial action, reacting demonstrated the ability to remove nucleases such as
with their DNA, resulting in inactivation. The combination RNase and DNase as well as pyrogens when challenged
185/254 nm light oxidizes organic compounds, allowing for with known concentrations of the material. The Type
total oxidizable carbon levels of less than 5 ppb. 1 systems with UV/UF options produce reagent grade
Benefits water with resistivity up to 18.2 MΩ.cm, TOC of 1-5 ppb,
pyrogens <0.001 EU/mL and no detectable RNase,
• Effective method to prevent bacterial contamination
DNase or DNA.
• Oxidizes organics to produce pure water with
Benefits
low TOC levels
• Removes nucleases and DNA
Limitations
• Produces water with low TOC and pyrogen levels
• Will not remove ions, colloids and particulates
Limitations
• Must be used in the same system
• Barnstead GenPure, MicroPure, LabTower EDI,
Smart2Pure, Pacific TII, LabTower TII water Systems that utilize this technology
purification systems • Barnstead GenPure, MicroPure, and Smart2Pure
water purification systems
Lab International Water Standards
Water
101
What Does Type 1 Water Mean?

Reagent water has quantitative specifications that describe the level of purity for the water. These specifications
have been described by ASTM (American Society for Testing and Materials) D1193, ISO (International Organization for
Standardization) 3696 and CLSI®-CLRW. (Clinical and Laboratory Standards Institute-Clinical Laboratory Reagent Water).
The most commonly used standards, ASTM D1193-6, are summarized in the tables below.
ASTM Standards for Reagent Water

Measurement (unit) Type I Type II Type III
Resistivity (MΩ.cm) at 25 °C >18 >1 >4
Total organic carbon (ppb) <50 <50 <200
Sodium (ppb) <1 <5 <10
Chloride (ppb) <1 <5 <10
Total silica (ppb) <3 <3 <500
The ASTM standards are further subdivided into A, B and C.

These standards can be used in conjunction with the Type of water.
ASTM Standards for Reagent Water

Measurement (unit) A B C
Heterotrophic bacteria count (CFU/mL) 10/1000 10/100 1000/10
Endotoxin (units per mL) 0.03 0.25 n/a
Here are some additional definitions of the parameters they use to indicate the water’s purity.
Resistivity – the tendency of Conductivity – the tendency of Total Organic Carbon (TOC) –
water without ions to resist water that contains ions to a measure of the organic
conducting electricity. conduct electricity. contaminants found in water.
The unit of measure is megohm- The unit of measure is microsiemens/ The unit of measure is parts per
centimeter (MΩ.cm), often shortened centimeter (µS/cm) or microhm/cm. million (ppm) or parts per billion (ppb).
to MΩ or “meg”. It is generally used The measurement is used to measure Feed water can be in the 2-5 ppm
for high purity water. The theoretical feed water or lower quality treated range and the best high purity water
maximum is 18.2 MΩ.cm at 25 °C. water. The more ions present in the should be in the 1-5 ppb range.
The higher the ionic content – the water, the higher the conductivity. This Measurement of TOC is done using
lower the resistivity and the lower the is measured by a conductivity meter. an in-line system.
ionic content – the higher the resistivity
(high resistivity is good!). In ultrapure
water systems this value is determined
using an in-line meter. Conductivity
and resistivity measurements are
inversely related to each other.
14
Advanced Technology Used to Monitor Purity
Water full of ions Pure water has very

will conduct few ions, so it does not
electricity well. conduct electricity well.
Conductivity Resistivity Conductivity Resistivity
Conductivity and Resistivity

Electrical conductance or resistance is measured by two in-line electrodes. Electrical current moves through water using
ionic molecules as stepping stones. The fewer stepping stones, the more difficult the passage of electricity. This causes
less electrical conductance and more electrical resistance. The temperature of the water also affects the conductivity/
resistivity so readings are usually normalized to 25 °C via temperature compensation.
We use resistivity to measure ion concentration in pure water. We understand water and realize that in order to achieve
Type 1 water, meeting the most stringent requirements of our customers, multiple technologies must be used.
Lab Building Your Water System
Water
101
16
What Type of Water Do You Need?

When designing your water system, we suggest you take time to evaluate the applications and interest
areas of the lab now and in the future. Take a look at the table below to determine what type(s) of water
you need.
Type of Water
Cartridge
Ultrapure Pure
Application and interest areas RO and filter
Type 1 Type 2
systems
General Lab Purpose
Autoclave • • •
Humidification • • •
Glassware washing/rinsing • • •
General lab equipment (water baths, incubators, etc.) • • •
Feed water to Type 1 systems •
Media prep •
Buffer prep •
Chemical and biochemical reagent prep •
Analytical
High performance liquid chromatography (HPLC) •
Gas chromatography (GC) •
Ion chromatography (IC) •
Inductively coupled plasma spectroscopy (ICP) •
Mass spectroscopy (MS) •
Atomic absorption (AA) •
Total organic carbon (TOC) •
Life Sciences
Genomics (ex. PCR, mutagenesis) •
Proteomics (ex. crystallography, electrophoresis) •
Immunology (ex. monoclonal antibody production, blots) •
Pharmacology •
Cell and tissue culture •
Drug discovery •
Molecular biology •
Microbiology •
Water
101
What Barnstead Water Systems Provide

Our water systems are engineered to be used
independently or in conjunction with our other water
systems for more flexibility. Below is a summary of
our product lines for each water type.
Barnstead GenPure
water purification system
Barnstead Smart2Pure
Ultrapure (Type 1) Water Pure (Type 2) Water

This grade of water is required for your most sensitive Type 2 water is most widely used in general laboratory
and critical laboratory applications. Type 1 water can be applications, such as reagent preparation and glassware
made pyrogen-, nuclease-, bacteria- and particulate-free. rinsing. It is also commonly used to feed our Type 1
It has the highest purity, but is also the most expensive systems to create a comprehensive water system.
to generate. The below systems provide you with the Type 2 water
If your applications require different types of water, let our you need for your applications.
experts assist with designing a water purification system • Barnstead Pacific TII water purification system
specific to your needs.
• Barnstead LabTower TII water purification system
The below systems provide you with the Type 1 water
• Barnstead Mega-Pure Glass Stills water purification
you need for your applications.
system
• Barnstead LabTower EDI water purification system
• Barnstead Classic Stills water purification system
• Barnstead Smart2Pure water purification system
• Barnstead GenPure water purification system
• Barnstead MicroPure water purification system
• Barnstead E-Pure water purification system
The ultrapure systems differ in their features and daily
volume output, so please see the Type 1 Section for
complete information.
18
Barnstead GenPure xCAD Plus water purification system, shown with two additional remote dispensers
Reverse Osmosis (RO) Water Cartridge and Filter Systems

RO is a percent removal technology, thus the purity of the Cartridges are a great option for laboratories either needing
product water is directly dependent on the quality of the point of use polishing of pretreated water or single state
feed water. Accordingly, it can not be generally classified as treatment of tap water.
a specific “type” of water. The below cartridge and filter systems are available for
RO water is recommended for use in general laboratory your specific application needs.
equipment such as the sterilizer, dishwasher and • Water system
water baths.
• Barnstead Hose nipple cartridges
The below systems provide you with the RO water you
• Bantam deionizer
need for your applications.
• Barnstead 1/2 Size B-Pure filters
• Barnstead Pacific RO water systems
• Barnstead LabTower RO water systems
Barnstead Hose Nipple Barnstead B-Pure Barnstead Bantam

Cartridge and Holder Cartridge Holder Deionizer
Water
101
Pretreatment Barnstead Pacific TII 30 L Polyethylene Barnstead GenPure

water purification system Reservoir xCAD Plus UV/UF water
purification system
Tap
water
Type 2 water Reservoir
flows into water feeds
the reservoir the Barnstead
Type 2 GenPure Type 1
Water Water
Watson Lab
Applications in the Lab : Cell and Tissue Culture, PCR, Media Prep and HPLC
Water Type(s) Needed : Type 1 (low organics, pyrogens and nucleases) and Type 2 water
Solution : Barnstead Pacific TII, 30 L reservoir, and Barnstead GenPure xCAD Plus UV/UF
The Watson Lab is a traditional cell and molecular biology lab, so they needed a system that is capable of producing
Type 1 water free of nucleases, pyrogens and bacteria. Our Barnstead GenPure xCAD Plus UV/UF system is perfect
for their ultrapure water needs.
Our Barnstead Pacific TII, which will produce water for the general lab uses, will also serve as feed water for the
GenPure xCAD Plus UV/UF.
Pretreatment Barnstead LabTower TII Barnstead GenPure Pro UV

water purification system water purification system
Tap
water
Barnstead LabTower
TII reservoir
water feeds the
Type 2 GenPure Pro Type 1
Water Water
Le Lab
Applications in the Lab : ICP/MS, GC/MS, HPLC, Chemical and Buffer Prep
Water Type(s) Needed : Type 1 (with low organics) and Type 2 water
Solution : Barnstead LabTower TII and the Barnstead GenPure Pro UV
The Le Lab performs analytical experiments on a daily basis. They would like to make their own buffers and reagents,
so we suggested a configuration that would give the lab both Type 1 and Type 2 water.
Our Barnstead LabTower TII converts tap water into Type 2 water, which is stored in the 100 L integrated reservoir.
Water can be drawn from the reservoir or from our Barnstead GenPure Pro UV (which has a 0.2 µm on it to ensure
bacteria-free water). The reservoir feeds our Barnstead GenPure Pro system, which will deliver ultrapure (Type 1) water.
20
Our water systems are designed to mix and match, as many laboratories have
Mix and Match for
the need for multiple types of water. Here are some examples of our systems
Greater Flexibility built for use in labs with diverse interest areas.
Dual Cartridge Barnstead Mega-Pure Still with Barnstead MicroPure UV

Deionizer Automatic Collection System water purification system
Distilled water
feeds the
MicroPure
Still water
Tap
flows into
water
the reservoir
Type 2 Type 1
Water Water
Bauer Lab
Applications in the Lab : General lab use, rinsing of glass and plastic ware, media prep, HPLC
Water Type(s) Needed : Type 1 and Type 2 (distilled)
Solution : Barnstead Mega-Pure Still with Automatic Collection System and a
Barnstead MicroPure UV
The Bauer Lab needed Type 1 water for their HPLC and analytical experiments (estimated to be less than 15 L a day)
and distilled water for general reagent preparation and glassware rinsing, thus our Barnstead MicroPure UV and a
Barnstead Mega-Pure Still with Automatic Collection System were recommended. A dual cartridge deionizer was also
suggested as pretreatment to the still as the lab’s tap water was especially hard.
Pretreatment Barnstead Smart2Pure UV/UF

Tap
water
Type 1
Water
Simpson Lab
Applications in the Lab : Protein purification and PCR
Water Type(s) Needed : Type 1
Solution : Barnstead Smart2Pure UV/UF
Biochemical techniques such as protein purification and PCR are important for the Simpson Lab, but they have
serious space constraints. Our Barnstead Smart2Pure UV/UF is the all-in-one solution for tap to Type 1 water.
It converts tap water to ultrapure water and has a small foot print. The UV/UF option will provide the lab with nuclease
free water, perfect for their sensitive applications.
Type 1
Ultrapure water
purification systems
What sets Barnstead water purification
systems apart?
Our Barnstead water purification portfolio includes
a complete line of Type 1 systems to meet all your
ultrapure water needs. Why so many options?
For you to have a tailored system specifically for
your needs. No need to pay for technologies that
you won’t use!
22
Type 1
Innovation
Ultrapure Water Purification Systems

UV and feed water monitoring
for maximum efficiency.
EDI systems with reservoir
recirculation maintains purity
even between use.
Convenience
Quick-change cartridge design
simplifies self maintenance.
All required parts and accessories
ship under one part number.
Flexibility
Full system control at the
Barnstead GenPure xCAD Plus
remote dispenser.
Feed water
Type 1
Barnstead ultrapure water purification system selector guide
Select the water system that

best fits your requirements Benefits
UV oxidation is necessary for the removal of organics in the

Ultra-low organic levels, <5 ppb
feed water, reduces organic levels to >5 ppb, and protects
HPLC, TOC, GC/MS, ICP, ICP-MS
against microorganisms
Cell culture, monoclonal antibody

Applications Ultrafiltration removes pyrogens from the feed water
production, electrophoresis
Nuclease and pyrogen-free applications

UV/UF is the most common combination of technologies –
such as PCR, 2-D electrophoresis,
used to reduce organics and remove pyrogens and nucleases
cell culture, blotting
Photosensor continually checks the intensity of the

UV monitoring UV lamp. A decrease could result in an incorrect TOC
measurement result.
Feed water monitoring alerts you to fluctuations in feed

Technology Feed water monitoring
water quality
Real-time monitor of the amount of organic materials in the

Total Organic Carbon (TOC) monitor
product water shown on the display
Dispense at the push of a button. Control from

Volumetric Dispensing
0.01-65 liters with an accurancy of <0.5 %
Allows for full control of the system at the GenPure xCAD Plus
GenPure xCAD Plus remote dispenser
remote dispenser
Features
Ideal when bench space is tight. System sits under the bench
Under-the-bench mounting
and out of sight.
RS-232 data printing at

Satisfies GLP guidelines, data available in print
pre-programmed intervals
Optimal amount of water to use daily to

Capacity Expands with your laboratory and application needs
sustain reasonable cartridge life
* Applicable when using pre-treated feed water only.
24
Tap water feed required
Type 1
Pretreated water feed required

GenPure xCAD
GenPure Pro GenPure MicroPure E-Pure LabTower EDI Smart2Pure
Plus
Full control at Produces
Smaller Compact unit
the GenPure Flexible Economical Simple design, Type 1 and 2
capacity for producing
xCAD Plus dispensing Type 1 water great value with EDI
optimal value Type 1 and 2
remote dispenser technology
3 3 3 3 — 3 3
3 3 3 3 — — 3
3 3 3 3 — — 3
3 3 3 — — — —
3 3 3 3 — — —
3 3 3 — — — —
3 3 — — — — —
3 — — — — — —
3 — — — — — —
3 3 3 — — 3 3
200 L/day 200 L/day 200 L/day 15 L/day 100 L/day* 200 L/day 70 L/day
Barnstead GenPure xCAD Plus water purification system
The Barnstead GenPure xCAD Plus system delivers ultrapure 18.2 MΩ.cm water with consistent quality for
the most demanding and sensitive applications. The xCAD remote dispenser provides flexibility and control.
Integrated feed water monitoring

All Barnstead GenPure systems exceed international
standards ASTM Barnstead Type I, ISO 3696 Grade 1, • Additional measuring cell monitors feed water conductivity.
ASTM D1193 and CLSI-CLRW. If the quality of the feed water drops below the set value, a
fault message will immediately be displayed.
Simultaneously dispense from up to three dispensers
Online TOC measurement with UV intensity monitoring
• Each system ships with a single xCAD Plus
remote dispenser • Real-time TOC monitoring for continual measurement of
organic substances present in the water
• For increased flexibility, add one or two more
Barnstead GenPure xCAD Plus dispensers • Continuous monitoring of UV intensity removes the risk of
false TOC values due to diminished UV intensity
• Dispense from all three simultaneously
• Fault message alerts user when the UV lamp intensity is
no longer sufficient for accurate TOC measurements
UV Photo-oxidation 185/254 nm
• High performance UV assembly reliably reduces the
content of microorganisms and their metabolites
• UV oxidation also reduces organic compounds in the
water to ultra-low levels
Leak detection
• Internal leaks can be detected and an error displayed
Ready-to-use
• Wall bracket, UV lamp, ultrapure cartridge, ultrafilter
(select models), sterile filter, all included in one part
number
Measurement of conductivity/resistivity
• Conductivity cells are carefully calibrated prior to each
measurement via built-in reference resistance with cell
Applications Include: constants at 0.01 cm-1
Molecular Biology and • Temperature measurements are made by a platinum chip
Microbiology sensor with ±0.1 °C accuracy
• Cell and tissue culture Quick-connect cartridge replacement
• PCR, DNA sequencing
• Aquastop quick-connect capability enables cartridge
• Electrophoresis
replacement within seconds even mid-operation
Analytical Chemistry Exact dosing
• HPLC • Electronic dosing for fully automatic volume control of
• GC, GC-MS, ICP-MS, AA 0.01-65 L with <2 % accuracy
• TOC Measurements, IC
26
Type 1
Full control

extreme flexibility
GLP-compliant documentation • Digital microprocessor control automatically
• Real-time clock and code-protected operating system monitors and stores faults from the last
prevents unauthorized changes to system settings four weeks
• RS-232 interface with adjustable send-interval for safe • USP-compliant conductivity measurement with
data transfer of all measured data, faults, date and time temperature compensation can be switched on
to a PC computer or log printer or off
Quick Look Comparison

GenPure xCAD Plus GenPure xCAD Plus GenPure xCAD Plus GenPure xCAD Plus
UV UV/UF UV - TOC UV/UF - TOC
system system system system
Molecular biology, Biosciences
Inorganic and organic Chemical analysis
PCR, DNA, (cell and tissue
trace analysis, (trace analysis,
Applications monoclonal culture media, PCR,
HPLC, ICP-MS, IC, HPLC, IC, ICP-MS,
antibodies, DNA, monoclonal
TOC analysis TOC measurements
cell culture media antibodies)
Resistance at 25 °C,MΩ.cm 18.2 18.2 18.2 18.2
Conductivity, µS/cm 0.055 0.055 0.055 0.055
TOC, ppb 1-5 1-5 1-5 1-5
RNase, ng/ml n/a <0.003 n/a <0.003
DNase, pg/µl n/a <0.4 n/a <0.4
Bacteria, CFU/mL <0.01 <0.01 <0.01 <0.01
Particles, 0.22 µm/mL <1 <1 <1 <1
Endotoxins, EU/mL n/a <0.001 n/a <0.001
TOC monitor n/a n/a standard standard
Flow rate, L/min up to 2 up to 2 up to 2 up to 2
Visible ultrapure water quality

Important measured values of conductivity/resistivity and TOC are easy to see during dispensing.
Non-Stop:
System ready
for dispensing
18.2 MΩ.cm: Resistivity TC: temperature

of the ultrapure water compensation is on
17.5 °C: Temperature measured for

1.00 L: Adjustable
conductivity compensation
volume to be
dispensed
UV: UV lamp is switched on
1 ppb: TOC value of

the ultrapure water
Barnstead GenPure xCAD Plus water purification system (continued)
Our Barnstead GenPure xCAD Plus remote

dispenser shown left.
The GenPure system is under the bench
(not pictured) freeing up valuable bench space.
For increased flexibility, add up to two

more xCAD Plus remote dispensers.
Barnstead GenPure xCAD Plus remote dispenser – full system control in a compact design
• xCAD is short for “Extended Control and Dispenser.” The system provides more flexibility for ultrapure water
dispensing with an action radius of 32 inches (80 cm).
• Ergonomic dispensing arm provides precise volume dosing at the press of a button.
• Turned, height adjusted or extended towards your vessel, you’ll appreciate the flexibility of this dispenser.
• One-of-a-kind design provides access to all system controls at your fingertips.
• Specify bench-top or wall-mount xCAD Plus dispenser for the installation option of your choice. The small
footprint of the bench-top xCAD Plus allows for more bench space while the wall-mount version completely
frees up horizontal workspace.
• Adjustable controller makes handling more practical and provides a clear view of critical settings.
• In addition to flexible xCAD Plus installation options, GenPure systems can also be installed under the bench
or wall-mounted with included mounting brackets up to 2.5 m (8 ft) away from xCAD Plus dispenser.
28
xCAD Dimensions
Dimensions W x D x H in (mm)
Type 1
Benchtop xCAD 10.2 x 21 x 28.5 (260 x 530 x 725)
Wall mount xCAD 10.2 x 21 x 25.8 (260 x 530 x 655)
Feed Water Requirements*

Potable tap water, pretreated by reverse
Source
osmosis, ion exchange or distillation
Feed water conductivity,
<2
µS/cm
TOC, ppb max 50
Bacteria count, CFU/mL <100
Complete water Turbidity, NTU <1.0
purification system Temperature, °C 2-35
control via the Barnstead
Pressure, psi (bar) 1.4-87 (0.1-6)
GenPure xCAD Plus
remote dispenser * Please see user manual for complete list of feed water requirements.
Product Specifications
Operating
Electrical Power Feed water Dimensions
Flow rate pressure
requirements consumption connector W x D x H in (mm)
min/max
29-87 psi 90-240 V, 14.6 x 13 x 24
up to 2 L/min 0.1 kW ¾” NPT
(2-6 bar) 50/60 Hz (372 x 330 x 615)
GenPure xCAD GenPure xCAD GenPure xCAD GenPure xCAD

System Options
Plus UV Plus UV/UF Plus UV - TOC Plus UV/UF - TOC
GenPure System*
All systems include a wall with xCAD Plus
50136152 50136151 50136153 50136146
bracket, ultrapure polisher bench version
cartridge, sterile 0.2 µm
filter, UV lamp and/or with xCAD Plus
ultrafilter where applicable 50136170 50136169 50136171 50136172
wall version
Optional Accessories
xCAD Plus Remote
Dispenser bench version 50136494 50136494 50136494 50136494
Additional remote dispenser
for increased flexibility. Up to
2 additional dispensers can wall version 50136505 50136505 50136505 50136505
be added to a single system.
Disinfection cartridge
Reusable cartridge needed to clean the
09.2201 09.2201 09.2201 09.2201
water purification system. Use with CMX25
cleaning solution.
Pressure Regulator
Recommended where incoming feed pressure 50155462 50155462 50155462 50155462
is above the specified range
Printer
Utilizes RS-232 interface for safe
documentation of all measured values STARA-106 STARA-106 STARA-106 STARA-106
and faults with date and time in compliance
with GLP guidelines
Replacement Consumables
Ultrapure polisher cartridge 09.2005 09.2005 09.2005 09.2005
Sterile filter, 0.2 µm 09.1003 09.1003 09.1003 09.1003
Ultrafilter n/a 50133980 n/a 50133980
UV lamp 09.2002 09.2002 09.2002 09.2002
Cleaning solution CMX25 CMX25 CMX25 CMX25
Barnstead GenPure Pro water purification system
The Barnstead GenPure Pro system delivers ultrapure 18.2 MΩ.cm water with consistent quality. Suitable for
the most demanding laboratory applications, this ultrapure water system will deliver water from a small footprint.
The GenPure Pro system can be used on a laboratory bench or on a wall. The new flexible dispenser offers
maximum freedom while dispensing ultrapure water up to 24 inches (60 cm) away from the unit.
All Barnstead GenPure systems exceed international Leak detection

standards ASTM Barnstead Type I, ISO 3696 Grade 1, • Internal leaks can be detected and an error displayed
ASTM D 1193 and CLSI.
Dispensing for a variety of tasks
• An additional measuring cell monitors feed water
• New flexible dispenser offers a radius of 24
inches conductivity. Any exceeding of the limiting value is
(60 cm) from the system for filling larger vessels or immediately displayed to maximize cartridge life.
glassware washing
Online TOC measurement with UV intensity monitoring
• Real-time TOC monitoring for continual measurement of
organic substances present in the water
• Continuous monitoring of UV intensity removes the risk of
false TOC values due to diminished UV intensity
• Fault message alerts user when the UV lamp intensity is
no longer sufficient for accurate TOC measurements
UV Photo-oxidation 185/254 nm
• High performance UV assembly reliably reduces the
content of microorganisms and their metabolites
water to ultra-low levels
Ready-to-use
• Wall bracket, UV lamp, ultrapure cartridge, ultrafilter
(select models), sterile filter, all included in one part
number
• Leak detection standard and will alert user to leaks
Applications Include: • Conductivity cells are carefully calibrated prior to each
Molecular Biology and constants at 0.01 cm-1
Microbiology
• Temperature measurements are made by a platinum chip
• Cell and tissue culture
sensor with ±0.1 °C accuracy
• Electrophoresis Quick-connect cartridge replacement
Analytical Chemistry replacement within seconds even mid-operation
• HPLC Exact dosing
• GC, GC-MS, ICP-MS, AA
• High precision POM draw-off valve for dispensing with
sterile filter
• Electronic dosing for fully automatic volume control of
0.01-65.0 L with <2 % accuracy
30
Type 1
Push button simplicity for easy dispensing
Flexible dispensing
precise purity monitoring
GLP-compliant documentation • USP-compliant conductivity measurement with
• Real-time clock and code-protected operating system temperature compensation can be switched on
prevents unauthorized changes to system settings or off
• RS-232 interface with adjustable send interval for safe Tilting Control Panels for Easy Viewing
data transfer of all measured data, faults, date and time • Ergonomically designed controller tilts for
to a PC computer or log printer ease-of-use and better visibility
• Digital microprocessor control automatically monitors • Illuminated four-line alpha-numeric display
and stores faults from the last four weeks

GenPure Pro GenPure Pro GenPure Pro GenPure Pro
system system system system
Molecular biology, Chemical analysis Biosciences
Inorganic and
PCR, DNA, (trace analysis, (cell and tissue
organic trace analysis,
Applications monoclonal HPLC, IC, culture media, PCR,
HPLC, ICP-MS, IC,
antibodies, ICP-MS, TOC DNA, monoclonal
TOC analysis
cell culture media measurements) antibodies)
Resistance at 25 °C,MΩ.cm 18.2 18.2 18.2 18.2
Conductivity, µS/cm 0.055 0.055 0.055 0.055
TOC, ppb 1-5 1-5 1-5 1-5
RNase, ng/ml n/a <0.003 n/a <0.003
DNase, pg/µl n/a <0.4 n/a <0.4
Bacteria, CFU/mL <0.01 <0.01 <0.01 <0.01
Particles, 0.22 µm/mL <1 <1 <1 <1
Endotoxins, EU/mL n/a <0.001 n/a <0.001
TOC monitor n/a n/a standard standard
Flow rate, L/min up to 2 up to 2 up to 2 up to 2
Barnstead GenPure Pro water purification system (continued)
Convenient dispensing for a variety of applications possible with flexible dispenser radius
Intuitive display shows important

system parameters
Quick-connect cartridge replacement
Exact dosing from Barnstead

GenPure Pro water purification
system with one touch dispensing
32
Source Potable tap water, pretreated by reverse osmosis, ion exchange or distillation
Type 1
Feed water conductivity, µS/cm <2
TOC, ppb max 50
Turbidity, NTU <1.0
Temperature, °C 2-35

* Please see user manual for complete list of feed water requirements.
Operating
Flow rate pressure
min/max
29-87 psi 90-240 V, 18.6 x 13 x 24.2
up to 2 L/min 0.1 kW 3/4” NPT
(2-6 bar) 50/60 Hz (472 x 330 x 615)
GenPure Pro GenPure Pro GenPure Pro GenPure Pro

System Options
GenPure Pro System*
All systems include a wall bracket, ultrapure
50131952 50131950 50131948 50131922
polisher cartridge, sterile 0.2 µm filter and UV
lamp and/or ultrafilter where applicable
Reusable cartridge needed to clean the
09.2201 09.2201 09.2201 09.2201
water purification system. Use with CMX25
cleaning solution.
Presure Regulator
Recommended where incoming feed 50155462 50155462 50155462 50155462
pressure is above the specified range
Printer
Utilizes RS-232 interface for safe
documentation of all measured values STARA-106 STARA-106 STARA-106 STARA-106
and faults with date and time in compliance
with GLP guidelines
Ultrapure polisher cartridge 09.2005 09.2005 09.2005 09.2005
Sterile filter, 0.2 µm 09.1003 09.1003 09.1003 09.1003
Ultrafilter n/a 50133980 n/a 50133980
UV lamp 09.2002 09.2002 09.2002 09.2002
Cleaning solution CMX25 CMX25 CMX25 CMX25
Barnstead GenPure water purification system
The Barnstead GenPure system delivers ultrapure 18.2 MΩ.cm water with consistent quality for the most
demanding and sensitive applications.
All Barnstead GenPure systems exceed international UV Photo-oxidation 185/254 nm

standard ASTM Barnstead Type I, ISO 3696 Grade 1, • High performance UV assembly reliably reduces the
ASTM D1193 and CLSI-CLRW. content of microorganisms and their metabolites
Integrated feed water monitoring • UV oxidation also reduces organic compounds in the
• An additional measuring cell monitors feed water water to ultra-low levels
conductivity. Any exceeding of the limiting value is Ready-to-use
immediately displayed maximizing cartridge life
• Wall bracket, UV lamp, ultrapure cartridge, ultrafilter,
sterile filter, all included with one part number
• Conductivity cells are carefully calibrated prior to each
constants at 0.01 cm-1
• Temperature measurements are made by a platinum chip
sensor with ±0.1 °C accuracy
Quick-connect cartridge replacement
replacement within seconds even mid-operation
GLP-compliant documentation
• Real-time clock and code-protected operating system
prevents unauthorized changes to system settings
• RS-232 interface with adjustable send-interval for safe
data transfer of all measured data, faults, date and time
to a PC computer or log printer
• Digital microprocessor control automatically monitors
• USP-compliant conductivity measurement with
Applications Include: temperature compensation can be switched on or off
Tilting control panels for easy viewing
Molecular Biology and
Microbiology • Ergonomically designed controller tilts for optimal key
• Cell and tissue culture pressing and easy readability
• PCR, DNA sequencing • Illuminated four-line alpha-numeric display
• Electrophoresis
Analytical Chemistry
• HPLC
34
Type 1
Basic configuration with

options to meet your needs
GenPure UV/UF system
Applications Molecular biology, PCR, DNA, monoclonal antibodies, cell culture media
Resistance at 25 °C, MΩ.cm 18.2
Conductivity, µS/cm 0.055
TOC, ppb 1-5
RNase, ng/ml <0.003
DNase, pg/µl <0.4
Bacteria, CFU/mL <0.01
Particles, 0.22 µm/mL <1
Endotoxins, EU/mL <0.001
TOC monitor n/a
Flow rate, L/min Up to 2
Barnstead GenPure water purification system (continued)
Barnstead GenPure systems feature tilting control panels for

optimal viewing
Easy cartridge replacement
36
Source Potable tap water, pretreated by reverse osmosis, ion exchange or distillation
Type 1
TOC, ppb max 50
Turbidity, NTU <1.0

Operating
Flow rate pressure
min/max
29-87 psi 90-240 V, 14.6 x 13 x 24.2
up to 2 L/min 0.1 kW ¾” NPT
(2-6 bar) 50/60 Hz (372 x 330 x 615)
System Options GenPure UV/UF

GenPure system*
All systems include a wall bracket, ultrapure polisher cartridge, sterile 0.2 µm filter, 50131217
UV lamp and/or ultrafilter where applicable
Reusable cartridge needed to clean the water purification system. 09.2201
Use with CMX25 cleaning solution.
Pressure Regulator
50155462
Recommended where incoming feed pressure is above the specified range
Printer
Utilizes RS-232 interface for safe documentation of all measured values and faults STARA-106
with date and time in compliance with GLP-guidelines.
Ultrapure polisher cartridge 09.2005
Sterile filter, 0.2 µm 09.1003
Ultrafilter 50133980
UV lamp 09.2002
Cleaning solution CMX25
*
Barnstead MicroPure water purification system
The Barnstead MicroPure system was designed for laboratories that require up to 15 L/day of ultrapure water
such as HPLC or other instrumental analyses, sample preparation, glassware rinsing, or molecular biology work
in which endotoxins and/or nucleases would interfere.
Compact system for

small ultrapure water demands
Smart features
• Backlit display tilts for easy viewing
• Variable speed dispensing
• Integrated 6 L feed water reservoir so you can place the
system without direct access to a water line (MicroPure
ST only)
Monitor feed water quality
• Good feed water quality leads to consistently ultrapure
water and a longer cartridge service life to maximize
cartridge life
Smart design
• Recirculation pump prevents bacterial growth during
standstill and allows for constant monitoring of all system
functions, allowing for the immediate display of fault
messages if a problem is detected
• Systems ship fully equipped with pressure reducer,
ultrapure cartridge, UV and 0.2 µm sterile filter
Superior filters
• Ultrafilter is flushed automatically to assure the highest
retention of endotoxins and nucleases, which produces a
Applications Include: long two year lifetime
• 0.2 µm final filter with folded membrane can be sterilized
Molecular Biology and
up to 5 times
Microbiology
• Cell and tissue culture UV Photo-oxidation 185/254 nm
• PCR, DNA sequencing • High performance UV assembly reliably reduces the
• Electrophoresis content of microorganisms and their metabolites
Analytical Chemistry water to ultra-low levels
• HPLC
38
Type 1
MicroPure UV system MicroPure UV/UF system
High-sensitivity instrumental analytical Microbiology, molecular biology,
Applications methods, such as HPLC, ICP-MS, PCR, DNA, monoclonal antibodies,
TOC-analyses cell culture media
Resistance at 25 °C, MΩ.cm 18.2 18.2
Conductivity, µS/cm 0.055 0.055
TOC, ppb 1-5 1-5
RNase, ng/ml n/a <0.003
DNase, pg/µl n/a <0.4
Bacteria, CFU/mL <0.01 <0.01
Particles, 0.22 µm/mL <1 <1
Endotoxines, EU/mL n/a <0.001
Flow rate, L/min 1.5 1
18.2 MΩ.cm water

in a compact system
Barnstead MicroPure water purification system (continued)
Dispense water which allows for drop One, two, three... The Barnstead MicroPure-ST series
by drop or quick dispensing Rapid cartridge change with includes a 6 L feed water reservoir for
Aquastop quick-connect design pretreated water storage
40
Source Potable tap water pretreated by reverse osmosis, ion exchange or distillation
Type 1
TOC, ppb max 50
Turbidity, NTU <1.0

Dimensions
Inlet Dimensions
Electrical Power Feed water MicroPure
Flow rate pressure MicroPure-ST
requirements consumption connector (no reservoir)
min/max W x D x H in (mm)
W x D x H in (mm)
up to 29-87 psi 90-240 V, 12 x 11.8 x 21.5 12 x 15.7 x 21.5
0.06 kW 3/4” NPT
1.5 L/min (2-6 bars) 50/60 Hz (305 x 300 x 545) (305 x 400 x 545)
System Options MicroPure UV MicroPure UV/UF

MicroPure system*
All systems include an ultrapure polisher cartridge, sterile 0.2 μm filter, 50132373 50132370
UV lamp and/or ultrafilter where applicable.
Wall mounting bracket 09.2212 09.2212
Pressure Regulator
Recommended where incoming feed pressure is above the specified 50155462 50155462
range
Reusable cartridge needed to clean the water purification system. 09.1102 09.1102
Only for line-fed units. Use with CMX25 cleaning solution.
MicroPure UV MicroPure UV/UF

System Options
with storage tank with storage tank
MicroPure with storage tank system*
All systems include a 6 L internal reservoir, ultrapure polisher
50132374 50132372
cartridge, sterile 0.2 μm filter, UV lamp and/or ultrafilter where
applicable
Pressure Regulator
Recommended where incoming feed pressure is above the specified 50155462 50155462
range
Replacement Consumables for MicroPure systems and MicroPure with Storage Tank System
Ultrapure polisher cartridge 09.1006 09.1006
Sterile filter, 0.2 µm 09.1003 09.1003
Ultrafilter n/a 50133981
UV lamp 09.1002 09.1002
Cleaning solution CMX25 CMX25
*
Barnstead E-Pure water purification system
The Barnstead E-Pure water purification system economically produces Type 1 water with low operating costs.
Large cartridges provide longer cartridge life and an impressive system flow rate.
Product Features Product Accessories

• Resistivities up to 18.2 MΩ.cm and TOC levels below Remote dispenser
10 ppb • Recirculates water through to tip of dispenser and
• Virgin polypropylene water pathways includes a 0.2 µm final filter
prevent recontamination • Delivers water up to 8 ft (2.4 m) from the system
• Recirculation pump continuously recirculates water • Ships with a mounting bracket for increased flexibility in
throughout the entire system to maintain water purity and choosing a mounting location
reduce the quantity of waste water required for rinse-up
Low pressure pump protector
• Wall-mountable and completely assembled for
• If you are feeding your E-Pure system from a pressurized
easy installation
feed line, it is to your advantage to ensure that the
• Quarter turn quick release canisters allow for easy pump is fully protected with the use of the low pressure
cartridge replacement pump protector
• 0.2 µm absolute final filter removes bacteria and • Interrupts power if pressurized feed water line pressure
particulates protecting the integrity of your results falls below 5 psig (0.35 kg/cm2)
• Digital resistivity meter is automatically temperature • Includes 6 ft (1.8 m) cord which plugs directly into
compensated to 25 °C, preventing fluctuating readings the E-Pure
due to temperature changes
• Complete with 1/4 inch NPT “T” to install in your feed line
• High-capacity cartridges provide low
operating costs Quick Look Comparison
Resistivity at 25 °C, MΩ.cm 18.2
TOC, ppb <10
Bacteria, CFU/mL <1
Flow rate, L/min 2
Applications Include:
Analytical Chemistry
• HPLC
Microbiology
• Buffer preparation
• Electrophoresis
42
Type 1
Simple to use
easy to maintain

Depending on the cartridge pack chosen – potable tap water, or water treated by
Source
reverse osmosis, ion exchange or distillation
Temperature, °C (°F) 4-49 (40-120)
Pressure, psig max gravity to 100
Feed water Inlet water
Overall dimensions W x H x D
connector temperature
E-Pure 3-holder 29 x 28 x 7.5 in (73.7 x 71.1 x 19.1 mm) 1/4 in NPTF 40-120 °F / 4-49 °C
E-Pure 4-holder 35 x 28 x 7.5 in (88.9 x 71.1 x 19.1 mm) 1/4 in NPTF 40-120 °F / 4-49 °C
Electrical
System Options Max Flow Rates 3-Holder 4-Holder
requirements
E-Pure systems 120 V 2 L/min D4631 D4641
Ships with wall bracket and
cartridge o-rings 240 V 2 L/min D4632-33 D4642-33
Required Accessories
D5028
D0835 Pretreatment (1)
Tap feed
D0803 High Capacity (1)
D5027 Ultrapure (2)
D5023
D0836 Macropure (1)
Tap feed,
D0803 High Capacity (1)
Cartridge kits Organic free
D5027 Ultrapure (1)
Choose the cartridge pack according to your D5021 Organic Free (1)
feed water and need for organic free water (Kits
include large canister o-rings) D50227
D5029
D0835 Pretreatment (1)
Pretreated feed D0835 Pretreatment (1)
D0809 Ultrapure (1)
D5027 Ultrapure (2)
D5027 Ultrapure (2)
D50228
D5022
D0836 Macropure (1)
Pretreated feed, D0836 Macropure (1)
D0809 Ultrapure (1)
Organic free D5027 Ultrapure (1)
D5027 Ultrapure (1)
D5021 Organic Free (1)
D5021 Organic Free (1)
0.2 µm final filter D3750 D3750
Remote dispenser
Recirculates water through to tip of dispenser and includes a 0.2 µm
D8952 D8952
final filter. Delivers water up to 8 ft (2.4 m) from the system. Ships with
a mounting bracket.
Low pressure pump protector
Alerts the E-Pure of an inadequate feed water condition in a D2706 D2706
pressurized feed line
Cleaning cartridges
D50223 D50223
Set of three.
Barnstead LabTower EDI water purification system
The Barnstead LabTower EDI is an integrated system combining purification with EDI technology and storage in
one, as well as producing both Type 1 and Type 2 water. Stock high purity water safely and conveniently!

Ultrapure water which exceeds ASTM Type I standards, Two systems in one
with a resistivity of 18.2 MΩ.cm and TOC 1-5 ppb with • Unique systems with pretreatment plus polisher to
standard UV lamp. High purity water ASTM Type II water, produce both Type 1 and Type 2 water
with resistivity of 10 - 1 MΩ.cm, is ideal for daily needs of
• Type 2 water is stored in the integrated 100 L high purity
100 to 200 L.
water reservoir
• Water system sits on top of the reservoir saving critical
bench space
Ready-to-use
• RO membranes, filters and cartridges, sterile filter,
all in one package
Two ways to draw water
• Dispense ultrapure water directly from the system via the
dispenser with sterile filter. The water quality is measured
immediately prior to the dispensing point.
• Type 2 water is accessible from the reservoir, which is an
ideal supply for lab equipment such as autoclaves
Performance for your application feeds
• High performance reverse osmosis module is paired with
an EDI module and polisher cartridge
• Two systems to choose from with capacities of
15 and 30 L per hour

LabTower LabTower
Applications Include: EDI 15 EDI 30
system system
Type 1 Water Applications Type 1 Water
• Electrophoresis, Resistivity at 25 °C, MΩ.cm 18.2 18.2
Conductivity, µS/cm 0.055 0.055
TOC Measurements, IC
TOC, ppb 1-5 1-5
• HPLP, GC-MS, ICP-MS, AA Bacterial content in CFU/mL <0.01 <0.01
Particles, 0.22 µm/mL <1 <1
Type 2 Water Applications Flow rate at dispenser, L/min 1.5 1.5
• Rinsing lab glassware Type 2 Water
• Supplying autoclaves and Pure water production at 15 °C L/hr 15 30
ultrapure water systems Resistivity at 25 °C 10-1 10-1
Conductivity 01-1.0 01-1.0
• Preparing and diluting buffers,
* Specification only applicable if system has optional ultrafilter attached.
reagents, and media
44
Dual quality system
Type 1 & 2
with EDI technology
Compact, stylish, mobile
• Free-standing unit takes up no bench space
• Easily relocated with bottom-mounted rollers

Safe operation
• Microprocessor control for automatic operation
• Continuous monitoring of all critical parameters
• Recirculation pump protects purified water from bacterial
growth during standstill
Quickly view reservoir level (volume in %)
GLP-compliant documentation with highly-visible continuous monitor
• Real-time clock and code-protected operating system

prevents unauthorized changes to system settings
• RS-232 interface with adjustable send-interval for safe
data transfer of all measured data, faults, date and time
to a PC computer or log printer
• Digital microprocessor control automatically monitors
• USP-compliant conductivity measurement with tempera-
ture compensation that can be switched on or off
100 L Integrated polyethylene reservoir safely stores
Type 2 water
• Automatic water recirculation in the reservoir moves
water across a special polisher module ensuring fresh
Type 2 water on demand
• High-purity water reservoir outlet for convenient drainage
• Efficient cleaning and disinfection enhanced by conical
bottom design for completely emptying reservoir
• Integrated reservoir provides 100 L of purified
water storage
• Sterile vent filter and reservoir overflow prevents
internal contamination by microorganisms from the
surrounding air
• Optional CO2 adsorber prevents CO2 adsorption into
the water, which would reduce cartridge life
• Highly visible reservoir volume display on
LabTower controller
• Customize volume stored in the reservoir via
the controller
Barnstead LabTower EDI water purification system (continued)
Project1_Layout 1 4/30/12 10:25 AM Page 1
Electrodeionization, EDI, unites two proven

technologies for producing ultrapure water:
Electrodialysis and ion exchange. In contrast
to conventional ion exchange in which resins
must be either chemically regenerated or the
cartridge discarded, EDI utilizes an electric
current for continual resin regeneration.
Technologies that keep you How EDI works

one step ahead Several layers of ion selective membranes are situated
• Constant high efficiency of the ion exchange layer bed between an anode and a cathode. Layered ion exchange
produces consistently high quality water resin beds and concentrate chambers are alternately
positioned between them.
• No regeneration chemicals required and no disposal
of cartridges, making this technology friendly for On applying an electric voltage, water (H20) is split into H+
the environment and OH – in the cell.
• No exchange of spent resins or cartridges saves cost The H+ and Na+ cations can migrate through the cation
permeable membranes, anions through the anion
• High-purity water when you need it with no wait time
permeable membranes.
The ions migrate in the direction of the applied voltage, i.e.
anions to the positive pole (anode), cations to the negative
pole (cathode). The water ions that migrate through an ion
exchange chamber displace salt ions retained by the ion
exchange resins and so continually regenerate the resins.
Optional hand dispenser The salt ions migrate through the appropriate ion selective
membranes into the concentrate chambers and are flushed
out by water. As all concentration chambers are flushed,
excess H+ and OH – ions can again combine to form H20.
46
Source Potable tap water softened or hardness stabilized
Type 1 & 2
Conductivity, µS/cm <1000
Colloid index <3
pH-range 4-11
Pressure, psi (bar) 29-87 (2-6)

Operating
Flow rate pressure
min/max
29-87 psi 90-240 V, 17.7 x 22.8 x 59.1
up to 1.5 L/min 0.25 kW 3/4” NPT
(2-6 bar) 50/60 Hz (450 x 580 x 1500)
System Options LabTower EDI 15 LabTower EDI 30

LabTower EDI system*
All systems include an ultrapure polisher cartridge, RO membrane,
RO pretreatment filters (5 µm filter and hardness stabilizer), EDI module, 50132395 50132396
UV lamp, sterile 0.2 µm filter, 10 inch 1 µm filter (reservoir outlet) and
pressure regulator
Sterile overflow for reservoir
50132714 50132714
Prevents the penetration of bacteria and other microorganisms
CO2 adsorber + sterile filter, 0.2 µm
Combination sterile filter with CO2 adsorber will prevent CO2 from entering 06.5002 06.5002
the tank, saving cartridge life
Mix Multi Mini water softener, 240 V
Required if feed water silt density index (SDI) is greater than 3. Also required
50130297 50130297
for purchase with a softener is the softener salt, hardness detection kit, and
the 5 μm filter with carbon.
Softener salt for Mix Multi Mini
50155490 50155490
Required for use with the water softener
5 μm filter with carbon cartridge
06.5201 06.5201
Required when Mixed Multi is purchased
Europe 06.1000 06.1000
Hardness detection kit – required with
purchase of softener North America/
Alerts user when water is no longer softened Latin America 50134335 50134335
Asia Pacific
Sterile 0.2 µm filter for reservoir outlet 06.5555 06.5555
Reusable cartridge needed to clean the water purification system. 09.2201 09.2201
Use with CMX25 cleaning solution.
Printer
Utilizes RS-232 interface for safe documentation of all measured values and STARA-106 STARA-106
faults with date and time in compliance with GLP-Guidelines
Hand Dispenser Kit
Hand dispenser with 3 meter cord that connects to tank (to dispense 50138221 50138221
Type 2 water). Ships with a 0.2 micron final filter.
Qualification documents (IQOQ) IOQDOCE50133916 IOQDOCE50133916
5 µm filter and hardness stabilizer 06.5204 06.5204
Sterile 0.2 µm filter for system 09.1003 09.1003
UV lamp for system 09.2002 09.2002
Reverse osmosis membrane 22.0046 (requires 2) 22.0087 (requires 2)
Barnstead Smart2Pure water purification system
All in one! The Barnstead Smart2Pure system is a compact system which converts tap water to into
ASTM Type I. The Smart2Pure 3 and 6 feature a built-in 6 L reservoir. The Smart2Pure 12 offers the choice of a 30
or 60 L reservoir for customizable storage capacity and ability to dispense both ASTM Type I and Type II water.
Powerful performance
• Based on your requirements, choose from capacities of
3, 6 or 12 L/hr
Lasting economy
• Water purification progresses through independent
cartridges with Aquastop quick-connect for fast replacement
Module 1 – Combination of pretreatment and the reverse
osmosis membrane
Module 2 – Polishing cartridge contains high-quality ultrapure
resin for consistent purity and long cartridge life
Easy-to-operate
• Dispensing is easy and features variable speed to control flow
• The display can be tilted for optimal reading
• Optional UV bulb and ultrafilter to customize ultrapure
water quality
Placement options
• Position on laboratory bench
• Mount it on the wall
Barnstead Smart2Pure 3 and 6 features smart, integrated
Applications Include: 6 L reservoir
Modular Biology and • Constructed with pigment-free materials
Microbiology
• Conical bottom outlet allows for complete draining and
• Cell and tissue culture
efficient cleaning and disinfection
• Electrophoresis Barnstead Smart2Pure 12 offers reservoir options, either
30 or 60 L
Analytical Chemistry • Large opening for easy and effective cleaning by hand
• HPLC • Sterile venting filter/reservoir overflow protects against
• GC,GC-MS, ICP-MS, AA contamination by microorganisms
• CO2 adsorber prevents TOC value increases from
drawn-in CO2
Routine Laboratory Work
• Preparing and diluting buffers • Polyethylene reservoir is opaque to light
and reagents • Recirculation pump protects the high purity water from
• Rinsing lab glassware bacterial growth during standstills and maintains the low
• Supplying autoclaves and other conductivity value
small lab equipment • Wall-mount optional for simple space-savings
• Conical bottom outlet allows for complete draining and
efficient cleaning and disinfection
48
Type 1 & 2
Compact dual
quality system

Smart2Pure UV system Smart2Pure UV/UF system
Type 1 Water
Inorganic and organic trace Molecular biology, PCR,
Applications analysis, HPLC, ICP-MS, DNA, monoclonal antibodies,
IC, TOC analysis cell culture media
Resistivity at 25 °C, MΩ.cm 18.2 18.2
Conductivity, μS/cm 0.055 0.055
TOC value, ppb 1-5 1-5
RNase, ng/ml n/a <0.003
DNase, pg/µl n/a <0.4
Bacterial content, CFU/mL <0.01 <0.01
Particles, μm/mL <1 <1
Endotoxines, EU/mL n/a <0.001
Flow rate, L/min Up to 1 Up to 1
Type 2 Water
Pure water production at 15 °C, L/h 12 12
Resistivity at 25 °C, MΩ.cm 15-10 15-10
Conductivity, µS/cm 0.067-0.1 0.067-0.1

Source Tap water
pH range 4-11
Temperature, C° 2-35
Conductivity (μS/cm), maximum 1200
Pressure, psi (bar) 1.4-87 (1-6)
Space-Saving Design
Simply stand the BarnsteadSmart2Pure system on the
benchtop or mount it directly to the wall, as shown by the
Thermo Scientific MicroPure, for more bench space
Barnstead Smart2Pure water purification system (continued)
Economical Design UV-Oxidation 185/254 nm Flexible

The RO membrane pretreatment cartridge is Optional UV-oxidation for reducing Gain increased flexibility by
separate from the DI cartridge, ensuring you the content of microorganisms adding a hand dispenser with
only replace each cartridge as needed and their metabolites 3 meter cord
Product Specifications – Storage Reservoirs

Dimensions
Volume Material
H x D in (mm)
23.5 x 15
30 L Polyethylene, (598 x 380)
ultrapure water resistant,
opaque to light 35.9 x 15
60 L
(912 x 380)
Storage Reservoir Options for

30 L 60 L
Smart2Pure 12 System Only
Tank
Choose a tank to meet your 50155497 50155501
capacity needs
Sterile overflow for reservoir 06.5001 06.5001
CO2 adsorber + sterile filter,
06.5002 06.5002
0.2 µm
Flexible Storage
Store high purity water safely and
conveniently in either a 30 or 60 L
reservoir (only for Smart2Pure 12)
50
Product Specifications - Smart2Pure 3, 6, 12 L/hr
Operating
Type 1 & 2
Flow rate pressure
min/max
29-87 psi 90-240 V, 12 x 15.7 x 21.5
2-6 bar 50/60 Hz (305 x 400 x 545)
Permeate flow Smart2Pure Smart2Pure

System Options for Smart2Pure
at 15 °C UV UV/UF
Smart2Pure System 3 and 6 L/hr* All systems
include an RO/pretreatment cartridge, ultrapure 3 L/hr 50129872 50129688

polisher cartridge, sterile 0.2 µm filter, UV lamp
and/or UF filter where applicable. The 3 and 6 lph
models also include an internal 6 L tank with 6 L/hr 50129885 50129887
vent filter.
Double cartridge pretreatment system
A 5 μm filter with activated carbon cartridge and a hardness stabilizer
09.4000 09.4000
cartridge ship complete with two 10 inch filter housings to control chlorine,
organic impurities and hardness.
Hand Dispenser Kit
Hand dispenser with 3 meter cord that connects to tank. Ships with a 50138221 50138221
0.2 micron final filter.
Smart2Pure 12 Smart2Pure 12
System Options for Smart2Pure 12
UV UV/UF
Smart2Pure System 12 L/hr*
Systems include an RO/pretreatment cartridge, an ultrapure polisher 50129890 50129845
cartridge, sterile 0.2 µm filter, UV lamp and/or UF filter where applicable
Smart2Pure Reservoir Choose a reservoir that fits your needs See "Storage Reservoir Options"
A 5 μm filter with activated carbon cartridge and a hardness stabilizer
09.4000 09.4000
cartridge ship complete with two 10 inch filter housings to control chlorine,
organic impurities and hardness.
Wall mounting bracket for the system 09.2212 09.2212
Pressure Regulator
50155462 50155462
Recommended where incoming feed pressure is above the specified range
Hand Dispenser Kit
Hand dispenser with 3 meter cord that connects to tank. 50138221 50138221
Ships with a 0.2 micron final filter.
Replacement Consumables - Smart2Pure 3, 6, 12 L/hr

3 L/hr 09.2003 09.2003
RO membrane with integrated pretreatment 6 L/hr 09.2006 09.2006
12 L/hr 09.2012 09.2012
Sterile 0.2 µm filter for system 09.1003 09.1003
Ultrafilter n/a 50133981
System UV-lamp 09.1002 09.1002
Cleaning solution CMX25 CMX25
Activated carbon cartridge with 5 μm prefilter 06.5201 06.5201
Hardness stabilizing cartridge 06.5452 06.5452
Sterile vent filter for tank 3 L and 6 L/hr 22.0091 22.0091
*
Barnstead Smart2Pure Pro Water Purification System
The Barnstead Smart2Pure Pro is a compact system that converts water into ASTM Type 1 and Type 2 water at a
rate of 16 lph in reverse osmosis, with built-in warning and shut-off features to prevent lab accidents.
Powerful performance
• Suitable for even the most demanding and sensitive
applications, the Thermo ScientificTM BarnsteadTM
Smart2PureTM Pro Water Purification System exceeds current
international standards ASTM D11931 Type 1, ISO 3696 Grade
1, and CLSI-CLRW, delivering ultrapure 18.2 MΩ.cm water with
consistent quality
• Offers production rate of up to 16 lph in RO.
Simplified user interface
• Easy system operation
• Enhanced system monitoring through integrated sensors and
timers for:
–– RO product purity
–– Leak detection and automated shut-off to prevent
accidents
–– Consumable monitoring dispensing is easy and features
variable speed to control flow
• The display can be tilted for optimal reading
• Includes UV bulb and ultrafilter to deliver ultrapure water quality
Lasting economy
Applications Include: • Independent cartridges to maximize life
Modular Biology and • Water purification progresses through independent cartridges

Microbiology with Aquastop quick-connect for fast replacement
• Cell and tissue culture Module 1 – Reverse osmosis cartridge to remove up to 99%
• PCR, DNA sequencing of tap water impurities
• Electrophoresis Module 2 – Polishing cartridge contains high-quality ultrapure
resin for consistent purity and long cartridge life
Analytical Chemistry Placement options
• HPLC • Position on laboratory bench
• GC,GC-MS, ICP-MS, AA
• Mount it on the wall
Easy ordering! One part number includes compete system
Routine Laboratory Work • Pretreatment to protect reverse osmosis membrane
• Preparing and diluting buffers • Smart2Pure Pro system to produce Type 1 and Type 2 water
and reagents complete with all consumables and wall bracket
• Rinsing lab glassware • Optional remote dispenser for flexible dispensing of Type 1 and
• Supplying autoclaves and other Type 2 water up to 3m from system
small lab equipment
• 30 or 60 L Storage tank to store and deliver Type 2 water
52
Type 1
Compact dual quality system

Features Barnstead Smart2Pure Pro offers reservoir
• Standby mode offers flexibility for use. The system options, either 30 or 60 liters
continually recirculates water to maintain purity • Sterile venting filter/reservoir overflow protects
• Built-in alert system helps ensure optimal performance with against contamination by microorganisms
prompts for leak detection and to replace consumables • CO2 adsorber prevents TOC value increases from
• System shuts off if a leak is detected drawn-in CO2
• Inline ultrafilter wtih large surface area to remove endotoxins • Recirculation pump protects the high purity water
and nucleases from bacterial growth during standstills and
• System parameters are code-protected to prevent maintains the low conductivity value
accidental changes to set points • Large opening for easy and effective cleaning by
Feed Water Requirements* hand
Potable tap water pretreated with • Conical bottom outlet allows for complete draining
Source Barnstead Pretreatment System -
Cat. No. 50157886 and efficient cleaning and disinfection
Silt density index (SDI) <3 • Polyethylene reservoir is opaque to light
Turbidity <1 NTU
Conductivity (reference
• Wall-mount optional for simple space-savings
1500 μS/cm (670 Ω·cm)
temperature +25°C)
Hardness Hardness stabilized
Chlorine Max. 0.1 ppm
pH range 4-11 Quick Look Comparison
Temperature +2°C - +35°C Smart2Pure Pro UV/
2 - 6 bar / 29 - 87 PSI (at a pressure Type 1 Water UF system
>6 bar / 87 PSI a pressure reducer Inorganic and organic
Pressure
must be installed upstream of the trace analysis, HPCL,
system. ICP-MS, IC, TOC
* Please see user manual for complete list of feed water requirements. Applications analysis, Molecular
biology, PCR, DNA,
monoclonal antibodies,
cell culture media
Resistivity at 25 °C, MΩ.cm 18.2
Conductivity, μS/cm 0.055
TOC value, ppb 1-5
RNase, ng/ml <0.003
DNase, pg/µl <0.4
Bacterial content, CFU/mL <0.01
Particles, μm/mL <1
Endotoxines, EU/mL <0.001
Flow rate, L/min Up to 1
Type 2 Water
Pure water production at 15
16
°C, L/h
Smart2Pure Pro
Resistivity at 25 °C, MΩ.cm 10-1
Conductivity, µS/cm 0.1-1.0
Barnstead Smart2Pure Pro water purification system (continued)
Economical Design UV-Oxidation 185/254 nm Flexible

The RO cartridge is separate from the DI UV-oxidation for reducing the Gain increased flexibility by
cartridge, ensuring you only replace each content of microorganisms and adding a hand dispenser with
cartridge as needed their metabolites 3 meter cord
Smart2Pure Pro Water System
Product Specifications – Storage Reservoirs

Dimensions
Volume Material
H x D in (mm)
23.5 x 15
30 L Polyethylene, (598 x 380)
ultrapure water resistant,
opaque to light 35.9 x 15
60 L
(912 x 380)
54
Product Specifications - Smart2Pure Pro
Electrical Feed water Dimensions
Type 1
Flow rate Power consumption
requirements connector W x D x H in (mm)
90-240 V, 12 x 15.7 x 21.5
50/60 Hz (305 x 400 x 545)

Barnstead Smart2Pure Pro ordering information
Description Cat. No.
Smart2Pure Pro UV/UF 16 LPH with 30 L Re-circulation Tank, Pre-Treatment and Wall Mount
Complete system includes Barnstead Pretreatment system with cartridges, RO/pretreatment cartridge, an
50157873
ultrapure polisher cartridge, sterile 0.1 μm filter, UV lamp, UF filter, 30 L tank, CO2 vent filter, sterile overflow and
system wall bracket. Tank wall mount sold separately.
Smart2Pure Pro UV/UF 16 LPH with 60 L Re-circulation Tank, Pre-Treatment and Wall Mount
Complete system includes Barnstead Pretreatment system with cartridges, RO/pretreatment cartridge, an
50157872
ultrapure polisher cartridge, sterile 0.1 μm filter, UV lamp, UF filter, 60 L tank, CO2 vent filter, sterile overflow and
system wall bracket. Tank wall mount sold separately.
Smart2Pure Pro UV/UF 16 LPH with 30 L Re-circulation Tank
Complete system includes RO/pretreatment cartridge, an ultrapure polisher cartridge, sterile 0.1 μm filter, UV 50157882
lamp, UF filter, 30 L tank, CO2 vent filter and sterile overflow.
Smart2Pure Pro UV/UF 16 LPH with 60 L Re-circulation Tank
Complete system includes RO/pretreatment cartridge, an ultrapure polisher cartridge, sterile 0.1 μm filter, UV 50157877
lamp, UF filter, 60 L tank, CO2 vent filter and sterile overflow.
Barnstead Pretreatment
50157886
Comes with pressure reducing valve, 1 micron prefilter, hardness stablizing cartridge and carbon cartridge
Optional Wall Mounting Bracket for POU Systems 50157885
Optional Hand Dispenser 50138221
Optional Wall Mount for 30 L Tank 06.5015
Optional Wall Mount for 60 L Tank 06.5016
Accessory/Consumables Description Cat. No. ** Included with new unit
1 micron prefilter D502113

Barnstead Pretreatment System Hardness stabilizer D502114
Carbon cartridge **50157375
Smart2Pure Pro RO membrane **50157608
Smart2Pure Ultrapure Cartridge **09.1020
Sterile 0.1 um filter for system **50157375
System Consumables 0.2 micron Final Filter for remote hand 09.1003
dispenser
Ultrafilter **50133980
UV Lamp for Type 1 System **09.1002
Reservoir Consumables CO2 Vent filter 06.5002
Type 2
Pure water
purification systems
Pure water for a wide range
of applications
Type 2 pure water is an essential part of creating
consistently pure chemical reagents, microbiological
buffers and media.
Type 2 water is also recommended as a
pretreatment for Type 1 ultrapure systems.
56
Type 2
Innovation
Pure Water Purification Systems

Advanced feed water monitoring
alerts user to fluctuations in feed
water quality to optimize cartridge
Convenience
Aquastop connections allow users
to quickly change the cartridge and
minimize downtime
Flexibility
Gain more space with numerous
installation options – on the bench,
wall or standalone
Type 2 Select the
system
Barnstead pure water purification system selector guide
Technology / Feature Benefits
Optimal amount of water to use daily to Take into account any new techniques you will introduce in
Capacity
sustain reasonable cartridge life the coming years or new volume demands you may have
Technology UV Oxidation Protects against microorganism
Automatic operation Total operation of the system is automatic
Features
Multiple choices of dispense Flexible options for dispensing
58
Type 2
Reverse osmosis
and deionization Distillation
Pacific TII LabTower TII

Mega-Pure Stills Classic Stills
water purification system water purification system
Multiple capacities
Flexible dispensing All glass design Tin-lined
available
7-20 L/hr 40 L/hr 1.4-6 L/hr 1.9-19 L/hr
Optional Optional — Optional for reservoir
Standard Standard Optional Optional
— Standard — —
Barnstead Pacific TII water purification system
The Barnstead Pacific TII water purification system converts tap water into high purity water. The ultimate system
for the automatic and economical production of Type 2 water, the Barnstead Pacific TII system supports daily
requirements from 20 to 200 liters.
Multiple options for Type 2 water

• Three system options with permeate flows of
7, 12 or 20 L/hr
• Space-saving design permits benchtop installation or
wall mounting with integrated mounting bracket
Operational reliability
• Microprocessor controlled for automatic operation with
continuous monitoring of all critical parameters
• Automatic return to “operating” mode ensures
recirculation during standstill periods
• Optional UV lamp prevents biological contamination
Easy-to-operate
• Resistivity/conductivity clearly displayed on large back-lit
control panel that tilts for optimum viewing
• Status of current operating mode clearly indicating
production, stand-by, or rinsing modes
• Reservoir fill-level shown as %
• System parameters are code-protected to prevent
accidental changes to set points
Routine Laboratory Work • Water is dispensed directly from storage tank
• Rinsing lab glassware GLP-Compliant documentation
• Supplying autoclaves and • Optional printer connects to RS-232 port for data
ultrapure water systems recording and traceability
• Cell constant of 0.01 cm-1 ensures precise
reagents, and media
conductivity measurements
• Temperature compensation for the conductivity
measurement has an accuracy of ±0.1 °C (in conformity
with USP 645)
Volume display
• Water level % in reservoir is shown automatically on the
Pacific TII display
Clear information
Large, illuminated four-line display is easy to read and
provides information on:
• Operating mode status, such as production or stand-by
• Reservoir volume in %
• Temperature in °C
60
Type 2
Type 2 water system
with ultimate flexibility
Pacific TII Pacific TII Pacific TII
7 L/hr system 12 L/hr system 20 L/hr system
Pure water production at 15 °C, L/hr 7 12 20
Resistivity at 25 °C, MΩ.cm 10-1 10-1 10-1
Conductivity, µS/cm 0.1-1.0 0.1-1.0 0.1-1.0
TOC, ppb <30 <30 <30
Removal, bacteria and particles, % 99 99 99
Silicate removal, % >99.9 >99.9 >99.9
Technologies that produce

high purity water —
Reverse osmosis membrane

The high performance reverse osmosis membrane
removes approximately 98 % of inorganic ions and
99 % of all dissolved organic substances as well as
microorganisms and particles.
High purity water cartridge
This cartridge further purifies the reverse osmosis
permeate to produce high purity water as required
by international standards (ASTM Type II, CAP, ISO
3696, BS 3997 and CLSI).
UV lamp (UV version only):
The 254 nm UV transmission ensures optimal
UV irradiation. Light of wavelength 254 nm acts
as germicide.
Recirculation
Recirculation of the water between the storage
reservoir and Pacific TII is important for
maintenance of the water quality. Water from the
reservoir is cycled through the high purity water
cartridge and the UV chamber, then back to the
reservoir. (available only for systems that include a
tank with recirculation/pressure pump).
Barnstead Pacific TII water purification system (continued)
The Barnstead Pacific TII system converts tap water to 10-1 MΩ.cm high purity water.
Barnstead Pacific TII reservoirs

• Store high purity water safely and conveniently!
Large opening
• For easy and effective cleaning by hand
Optional safety filters:
• A CO2 adsorber prevents conductivity increases from
drawn-in CO2
• Sterile overflow prevents microorganisms from entering
and contaminating reservoir water
Polyethylene reservoir
• Constructed with inert materials opaque to light
Recirculation pump
• Recirculation pump protects high purity water from
bacterial growth during standstill while maintaining a
low conductivity value
Wall mount
• Simple and space-efficient wall mounting
Conical bottom outlet
• Allows complete draining and efficient cleaning
and disinfection
Specifications and Ordering Information
Feed Water Specifications*
Source and pre-treatment Tap water, softened or hardness stabilized
Free chlorine concentration, mg/L <0.1
pH-Range 4-11
Temperature, °C 2-35 °C
* Complete list of feed water specifications can be found in the operational manual.
Pacific TII System

Operating Electrical Power Feed water Dimensions Ambient
pressure requirements consumption connector WxDxH temperature
29-87 psi 90-240 V, 14.6 x 13 x 23.7 in
0.08 kW 3/4” NPT 2-35 °C
(2-6 bar) 50/60 Hz (372 x 330 x 603 mm)
Storage Reservoir
Volume Material Dimensions
30 L 23.5 x 14.9 in (598 x 380 mm)
Polyethylene, ultrapure water resistant,
60 L 35.9 x 14.9 in (912 x 380 mm)
opaque to light
100 L 49.2 x 14.9 in (1249 x 380 mm)
62
System Options UV option Pacific TII 7 Pacific TII 12 Pacific TII 20
Pacific TII Systems System
Type 2
50132131 50132132 50131982
All systems have a built-in wall with UV
bracket and include an RO embrane,
a high purity water cartridge and UV System
lamp where applicable 50132123 50132124 50132125
without UV

Pacific TII Reservoir
See “Storage Reservoir Options” below
Choose a reservoir that fits your needs
A 5 µm filter with activated carbon cartridge and a
hardness stabilizer cartridge ship complete with two
09.4000
10 inch filter housings to control chlorine, organic impurities
and control hardness <250 ppm TIS
For full pretreatment options, see p. 86
Qualification documents (IQOQ) IOQDOCE50133915 IOQDOCE50133915 IOQDOCE50133915
Water watcher
16.0129 16.0129 16.0129
Alerts the user to leaks – available as 230 V, 50 Hz only
Printer
Utilizes RS-232 interface for safe 120 V, 50/60 Hz STARA-106 STARA-106 STARA-106
documentation of all measured values
and faults with date and time in 230 V, 50 Hz 09.2207 09.2207 09.2207
compliance with GLP-Guidelines
Hand Dispenser Kit
Hand dispenser with 3 meter cord that connects to tank.
Use only with a tank equipped with a pressure pump 50138221 50138221 50138221
(50155494, 50155499, 50155502). Ships with a 0.2 micron
final filter.
High purity water cartridge 09.4011 09.4011 09.4011
Reverse Osmosis membrane
22.0046
Pacific TII 3, 7, 12 only require a single RO membrane 22.0046 22.0046
(order 2)
Pacific TII 20 and 40 require two membranes as indicated
System UV lamp 50139226 50139226 50139226
Activated carbon cartridge with 5 µm prefilter 06.5201 06.5201 06.5201
Hardness stabilizing cartridge 06.5452 06.5452 06.5452
Storage Reservoir Options for Pacific TII 30 L 60 L 100 L

Reservoir with level display 50155495 50155500 50155503
Choose a tank to meet your capacity with level display and recirculation/
and pump 50155494 50155499 50155502
pressure pump
Sterile overflow for reservoir 06.5001 06.5001 06.5001
Sterile filter, 0.2 µm (ideal choice for
50135142 50135142 50135142
Vent filter tanks without a recirculation pump)
Choose the vent filter for your Sterile filter, 0.2 µm + CO2 adsorber
application needs (ideal choice for tanks with a 06.5002 06.5002 06.5002
recirculation pump)
Wall mounting bracket 06.5015 06.5016 na
Pure Water Distribution Manifold - 2 Outlet
50155463 50155463 50155463
(Optional for use with tanks with recirculation pump)
Pure Water Distribution Manifold - 3 Outlet
50155458 50155458 50155458
(Optional for use with tanks with recirculation pump)
Sterile 0.2 μm filter for hand dispenser 09.1003 09.1003 09.1003
Barnstead LabTower TII water purification system
The Barnstead LabTower TII converts tap water into high-purity water and provides water
storage for a complete
Type 2 water solution.
Consistent water quality Compact mobile design

• Type 2 laboratory water meets ASTM Type II, • Two system options with permeate flows of 40 L/hr
CSLS-CLRW and ISO 3696 international standards • Stand-alone design with integrated 100 L reservoir takes
• Designed to continuously recirculate purified reservoir up zero bench space
water to preserve water quality even during periods • Castors allow easy relocation
of inactivity
Two ways to dispense water
• Dispense directly from the system via the dispensing
valve for calibration solutions, reagent preparation,
filling of containers, general rinsing purposes, etc.
• Dispense from the reservoir to supply autoclaves,
dishwashers, ultrapure water systems, etc.
• Integrated 100 L reservoir
• Up to 100 L of high purity water ready for dispensing
when needed
• High purity polypropylene reservoir is opaque to light
• Conical bottom allows complete emptying for efficient
cleaning and disinfection
• Adjustable setting for reservoir volume can be
programmed for times when demand is low, allowing
you to only store water that you can use
Clear information on system display
• The large four-line display is code-protected to prevent
accidental changes in system settings
• Illuminated and easily readable, the display provides
information on resistivity or conductivity, operating mode
status, such as “production”, “stand-by” or sanitization,
and volume of the reservoir in %
• Developed to meet or exceed GLP requirements
• Recorded and traceable data can be obtained by
print-out via the RS-232 interface and accessory printer
• Highly qualified and precise measurement of the
• Rinsing lab glassware conductivity is ensured by the cell constant of 0.01 cm-1
• Supplying autoclaves and to an accuracy of ±0.1 °C
ultrapure water systems
reagents, and media
64
Type 2
Pure water system
with integrated 100 L tank
LabTower LabTower
TII TII + UV
system system
Pure water production at 15 °C, L/hr 40 40 Barnstead LabTower TII with
Resistivity at 25 °C, MΩ.cm 10-1 10-1 optional hand dispenser
Conductivity, µS/cm 0.1-1 0.1-1
Bacteria content, CFU/mL,
<0.01 <0.01
with sterile filter
TOC, ppb <50 <50
Particle content µm per mL
<1 <1
with sterile filter
Pretreatment
• 5 μm prefilter and activated carbon in the combi-filter
cartridge protects the RO membrane from chlorine
and particles
• Hardness stabilizing cartridge protects the reverse
osmosis stage from hard water
Reverse osmosis and reservoir
• Pretreated water is pressure-forced through the
permeable reverse osmosis module removing 97-99 %
of all inorganic ions, 99 % of dissolved organic
substances as well as microorganisms and particles
• The built-in 100 L reservoir has a conical bottom outlet to
optimize cleaning and sanitization
Ion exchange and UV
• RO membrane removes up to 99 % of impurities. All
remaining ions are removed by the low TOC, high-purity
resins in the ion exchange cartridge.
• Irradiation with UV light (TII units with UV option only)
eliminates any bacteria or germs that are present
www.thermofisher.com/purewater 65
Barnstead LabTower TII water purification system
66
Feed Water Specifications*
Type 2
Source Potable tap water softened or hardness stabilized
Blocking index (SDI) <3, with higher values, an upstream pretreatment (model no. 09.4000) is to be installed
Free chlorine, mg/L <0.1

Colloid index <3
pH-Range 4-11
LabTower TII
Operating Electrical Power Feed water Dimensions Ambient
pressure requirements consumption connector W x D x H in (mm) temperature
29-87 psi 90-240 V, 17.7 x 22.8 x 59
0.12 kW 3/4” NPT 2-35 °C
(2-6 bar) 50/60 Hz (450 x 580 x 1500)
S ystem Options UV option LabTower TII 40

LabTower TII Systems*
All systems include an RO membrane, a high purity System with UV 50132141
water cartridge, 10 inch 5 µm prefilter with hardness
stabilizer cartridge, pressure regulator, 06.5555 optional
0.2 10" filter, 0.2 µm filter, integrated 100 L reservoir and
System without UV 50132196
UV lamp where applicable
5µm filter with activated
50134022
LabTower TII Pretreatment carbon and a 10” filter housing
Both cartridges are required for complete pretreatment 1 µm filter with a
09.4003
10” filter housing
Sterile vent filter for reservoir 50135142
Sterile overflow for reservoir 50132714
Water watcher
16.0129
Alerts the user to leaks. Available as 230 V, 50 Hz only
Printer 120 V, 50/60 Hz STARA-106
Utilizes RS-232 interface for safe documentation of
all measured values and faults with date and time in
compliance with GLP-Guidelines 230 V, 50 Hz 09.2207
Qualification documents (IQ/OQ) IOQDOCE50134156
Hand Dispenser Kit
50138221
Hand dispenser with 3 meter cord that connects to tank. Ships with a 0.2 micron final filter.
High purity water cartridge 02.2850-LAB
Reverse Osmosis membrane 22.0087
LabTower TII 40 requires two membranes (must order 2)
System UV-lamp 50139226
Sterile 0.2 µm filter for hand dispenser 09.1003
Activated carbon cartridge with 5 µm prefilter 06.5201
Hardness stabilizing cartridge 06.5452
Sterile 0.2 μm filter for reservoir outlet 06.5555
5 µm prefilter and hardness stabilizer 06.5204
*
Barnstead Mega-Pure Glass Stills
Barnstead Mega-Pure Glass Stills effectively remove inorganic solids, organics with boiling points higher
than water, bacteria, and pyrogens. The stills are constructed of non-leaching components to ensure high
purity water.
Product Features
• Contact with only glass, quartz and Teflon®
components ensures ultimate purity and eliminates
cross-contamination
• Choice of three production capacities
• Units are easily wall- or bench-mounted
• Vertical condenser design provides maximum purity
• High temperature cut-off shuts down the still if the
temperature is too high, preventing heating
element burnout
• Quick release cover provides easy cleaning access
• Connection to Automatic Collection System (ACS)
assures a 24-hour automatic system
• Includes feed water solenoid valve for
automatic operation
MP-1
• Compact, easy-to-use 1 L/hr glass still available with
or without a storage bottle
MP-3A and MP-6A
• Ideal for larger volume demands with storage
and options

MP-1 MP-3A MP-6A
Compact,
Stills for larger volume
System features easy-to-
demands
use
Product water
1 3 6
capacity, L/hr
Cooling water
11.3 30.3 53
capacity, L/hr
Number of heaters 1 1 2
Routine Laboratory Work Resistivity at 25 °C,
1-10 1-10 1-10
MΩ.cm
• Rinsing lab glassware
• Supplying autoclaves and
ultrapure water systems
reagents, and media
68
Type 2
Glass stills ideal for

biological applications
Product Accessories
Automatic Collection System (ACS)
• All glass system designed to collect water from the
Mega-Pure Glass Stills and control their operation
• Generous 45 L capacity
• Easy wall- or bench-mount
• Preset to shut off the power to the heaters and the
water supply to the still after the bottle has collected
approximately 45 L
• The system will automatically restart the still and refill the
collection system when the purified water supply has
been depleted to approximately 38 L
Pretreatment for hard water

• Allows for pretreating feed water to Mega-Pure Plastic Bottle (413964)
Glass Stills
• B-Pure single or dual cartridge deionizer reduces scale
build up and increases distillate purity
• Greater flexibility in meeting varying feed water qualities
• Purity light measures water quality and indicates when
cartridges are exhausted
• Large cartridge capacity for extended cartridge use
• Unit must be wall mounted
13 L Bottle (413934)
Automatic Collection System Barnstead B-Pure

Cartridge Holder
Barnstead Mega-Pure Glass Stills (continued)

Inlet water Vent temperature Auto start/stop
Auto drain Inlet pressure (psig)
temperature °C (°F) °C (°F) controls
4-37 (39.2-98.6) 85-96.7 (185-206) No 20-100 Yes
S ystem Options

Volume Electrical (50/60 Hz) Model number
Overall dimensions
output 6 L bottle
W x H x D in (cm) Volts Amps Phase Unit
(L/hr)* included
MP-1** 18 x 34 x 9.75
1.4 120 9 1 A440266 A440267
(45.7 x 86.4 x 24.8)
23 x 45 x 12 240 11 1 A440367 –
MP-3A** 3.4
(58.4 x 114.3 x 30.5) 208 13 1 A440696 –
23 x 45 x 12 240 21 1 A440518 –
MP-6A** 6
(58.4 x 114.3 x 30.5) 208 25 1 A440697 –
Required Accessories Model Number

Dual solenoid MP-1 (240 V), MP-3, MP-6A 440236
Required if using pretreated boiler feed water and untreated (tap) cooling water MP-1 (120 V) RY798X2A
Storage solutions
See Storage Options
Optional Accessories Model Number

D4511 Single Bpure;
Single Cartridge Deionizer
331040 Adapter Kit;
Single cartridge deionizer removes inorganic contaminants. Use with adapter kit to
D0803 High Capacity Cartridge
connect to Megapure still. Wall mount only. 7 x 24 x 7 in (17.8 x 61 x 17.8 cm)
(Recommended)
Double Cartridge Deionizer D4524 Double Bpure with 50 Kohm light;
Provides increased capacity to remove inorganic contaminates. Suggested combination - 331041 Adapter kit;
(2) High Capacity cartridges. Use with adapter kit to connect to MegaPure still. D0803 High Capacity Cartridge
Wall mount only. 15 x 27 x 7 in (38.1 x 68.65 x 17.8 cm) (Qty 2 Recommended)
High purity cartridge
D0809
Used in the Bpure unit for higher purity
High capacity cartridge
Deionization cartridges Used in Bpure. Removes ionized solids for D0803
extended capacity
Organic removal cartridge
D0813
Used in Bpure for organic and chlorine removal
* Volume Output for 208 V units can be reduced up to 25 % less than specified. **
70
Storage Options Model Number
Plastic bottle, 6 L capacity
413964
Type 2
Storage bottles For use with MP-1 and MP-3A stills
For manual operation Glass bottle, 13 L capacity
413934
For use with any Mega-Pure Glass Still
Automatic collection system Automatic collection system (ACS) unit B440704
(ACS)
For use with any Mega-Pure Flexible tubing adapter kit

Glass Still. Needed when the supplied glass tubing is either too long or too
440138
Collects up to 45 L of water and short to reach still. Flexible tubing is not included. 0.5 inch Teflon
controls the still. tubing able to handle 80-90 °C water is recommended
Dimensions: W x H x D ACS wall mounting bracket 400634
16.25 x 38 x 16.25 in
(41.3 x 96.5 x 41.3 cm) Adapter for ACS washer hook-up for glassware washers 440241
Barnstead Classic Stills
Barnstead Classic Stills effectively remove inorganic solids, organics, bacteria, and pyrogens. The stills are
constructed of copper and bronze with a coating of pure tin. The inert nature of tin prevents any leaching of
contaminants into the water.
Product Features • Units are easily floor and bench mountable, depending
on model
• Double-walled boiler and preheating of feed water
conserves electricity • Inert pure tin pathways assure product water quality
• Vented condenser allows for stripping of • Controller unit can be wall mounted
gaseous impurities Portable Stills
• Unique de-concentrator removes scale forming • Requires no permanent plumbing or electrical
impurities from the boiler connections, allowing quick set-up
• Unique Thermo Scientific Q-Baffle ensures high quality • Includes low water cut-off for safety protection
pyrogen free water by stripping contaminant laden water • Bench-mounted
droplets from steam
Floor Model Electric Stills
• Space-saving horizontal condenser
• Come standard with floor mounting stand
• Metal construction withstands years of use
• New over temperature protection system improves safety
• New plug-and-play hardware allows for easier installation
• Capacities from 2 to 5 gal/hr
• Low water cut-off protects heating elements when water
supply is interrupted
• Optional fully automatic controls allow for
unattended operation
–– Stops still when storage reservoir is full, starts still
when water level falls below preset level
–– Drains still boiler at selected 4-hour intervals to reduce
scale formation
Steam Still
• Utilize available in-house steam as heat source
• Optional automatic controls allow for
unattended operation
–– Stops still when storage reservoir is full, starts still
when water level falls below preset level
–– Drains still boiler at selected 4-hour intervals to reduce
Routine Laboratory Work scale formation
• Supplying autoclaves and Quick Look Comparison
ultrapure water systems Portable Floor Model
Electric Stills Electric Stills
Product water
reagents, and media 1/2 2 5
capacity, gal/hr
Mounting Bench Floor
72
Type 2
Tin lined

for pure water
Tin-Coated Storage Reservoir Specifications
Cooling Electrical
Output (50/60 Hz)
water Dimensions Model
gal/hr KW Phase
gal/hr W x H x D in (cm) number
(L/hr)
(L/hr) Volts Amps
Electric 1/2 gal/hr
0.5 (1.9) 4 (15) 1.3 120 12 1 21 (52) 19 (49) 10 (26) A1007
stills* Portable
Floor stand 2 (7.6) 16 (61) 6 240 26 1 23 (58) 68 (173) 11 (28) A1013-B-61
2 gal/hr
included 2 (7.6)** 16 (61) 6 208 17 3 23 (58) 68 (173) 11 (28) A1013-C
(except 5 (19) 40 (151) 13 240 57 1 35 (89) 77 (196) 14 (36) A1015-B-61
A1007) 5 gal/hr
5 (19)** 40 (151) 13 208 36 3 35 (89) 77 (196) 14 (36) A1015-C
** Volume Output for 208 V units can be reduced up to 25 % less than specified.
Required Accessories Model Number

Storage reservoir Storage of distilled water is required, see pages 70-71 for storage tank and accessories
Fully automatic controls
120 V (50/60 Hz) includes For use with 1 and 2 gal/hr electric stills G2100
feed water and drain solenoid
valves, manual valves, piping For use with 5 gal/hr electric stills G2110
and level monitor
*
Barnstead Classic Still Storage Reservoirs and Accessories
Select a Barnstead Classic Still Storage Reservoir and mounting option that will best fit your needs. The Thermo
Scientific Barnstead Ventgard Air Filter, Water Seal and UV Light Assembly are accessories that will protect your
pure water during storage.
Storage reservoirs
• Available in 25 G gallon reservoir
• Crafted from copper and hand-wiped with pure tin to
ensure the purity of the distilled water
• All reservoirs include a removable cover, water level
sight glass and a tin coated draw-off faucet with serrated
hose nipple
• Connections provide for optional accessories including
pumps and fully automated controls
• Requires a floor stand or wall bracket for
proper installation
Floor stand and wall bracket
Choose either a floor stand or wall bracket for proper
installation of reservoir
Floor stands
• 25 reservoir stand is fabricated from heavy gauge sheet
metal and has adjustable feet
Wall brackets
• 25 gallon reservoir brackets are fabricated of thick
steel angle-iron
Ventgard air filter and water seal
• Protects the stored water against airborne particulates,
organics and CO2
• Water seal works as a one-way valve to allow distilled
water into reservoir
• Prevents air from entering the reservoir via the condenser
atmospheric vent
UV light assembly
• Maintains sterility in the reservoir
• UV lamp is enclosed by a tubular, transparent,
inert sheath
• When lamp changes are necessary, only the lamp needs
Storage Reservoir Storage Reservoir
to be removed. The reservoir cover and sheath remain in
Floor Stand Wall Bracket place eliminating any exposure of water to contamination
from the air.
74
Type 2
System Options
Distribution outlet Drain outlet Overall dimensions in (cm)
Reservoir options Model number
(NPT) (NPT) W H D
25 gal* 1/2 in n/a 18 (46) 29 (74) 25 (64) B3045
Mounting options For the 25 gal
Choose the Floor stands 19 (48) 27 (69) 19 (48) H1001
reservoir
mounting option
that best suits Wall brackets For the 25 gal reservoir H3242
your needs.
Ventgard air filter and water seal H3111
Optional Accessory
UV light assembly
H4005
Maintains water sterility in reservoir. Includes housing, on/off switch, 6 ft. cord, lamp and ballast
Replacement UV lamp 04141
Ventgard air filter
H3120
Protects stored water against airborne particulates, organics and CO2
Replacement water seal
H3130
Prevents air from entering storage reservoirsvia condenser atmospheric vent
Ventgard filter element
25001-DB
The consumable portion of the Ventgard filter assembly
*
Distillation 101
Distillation effectively removes most inorganic solids, all organics with a boiling point greater than water (100 °C ),
all bacteria and pyrogens. Gases and low molecular weight organics are not effectively removed by distillation.
Thermo Scientific Barnstead products

include a broad range of stills
Our stills range in size from 1.4 to 38 L/hr. Select from
stills constructed of tin or glass. Many models offer Step-By-Step technologies used in distillation systems
fully automatic options or pretreatment options to
minimize scaling. 1. Feed water enters the still through the Cooling Water/
Feed Water Inlet. As the water passes through the Inlet,
it becomes warmed as it makes its way to the boiler.
Still components It in turn cools the vapor entering the condenser.
A still includes a boiling chamber (boiler), electric or steam
2. Water flows from the condenser into the constant level
immersion heaters, pyrogen reducing baffle, condenser,
device, then into the boiler.
constant level device and low water cutoff. Options include
pretreatment solutions and fully automatic controls that 3. The water in the boiler is heated. Impurities with a
allow stills to work automatically with pretreated feed water boiling point higher than water (100 °C / 212 °F)
and a storage reservoir. remain in the boiler, while water and impurities with
a boiling point equal to or lower than water are
converted to water vapor. The pure vapor moves up
the boiler through a pyrogens-reducing baffle and into
the condenser.
4. In the condenser, the pure vapor is transported

through the condenser where it contacts tubes or coils
containing cooling water. The pure vapor contacts
these tubes and coils and is condensed to produce
pure water.
5. The distilled water exits the condenser and is stored in

a reservoir through the Distilled Water Outlet.
6. Atmospheric vent allows for volatile contaminants to be

vented, increasing the purity of the distilled water.
76
Type 2 Pure Water Purification Systems
77
thermofisher.com/purewater
3
2
4
5
6

Reverse osmosis
Water purified using reverse osmosis is useful in
supplying water to lab equipment such as water
baths, dishwashers and autoclaves.
78
RO Systems
Innovation
LabTower RO system allows the

tank level to be set manually
Convenience
Dispense easily from the tank
Flexibility
Endless mounting choices – on the
bench, on the wall, or free-standing
Reverse
osmosis
Reverse osmosis is economical are flushed away to the drain. The result is purified product
Reverse osmosis is the most economical method to water free of up to 99 % of its impurities. The specific
remove up to 99 % of impurities in feed water. The RO rejection rates of all the impurities are shown in each of the
membrane is semi-permeable with a thin microporous product specifications.
surface that rejects virtually all dissolved materials including Feed water factors
inorganic solids, organics, particles and microorganisms. Certain characteristics of your feed water directly relate
Reverse osmosis water is versatile not only to membrane performance and lifetime but also
RO water can be used to prepare microbiological buffers directly impact the quality of your product water.
and chemical reagents. Additionally, it is a great option for Take advantage of our Thermo Scientific H2O Select
use in general laboratory equipment, such as water baths, Analysis Kit, our free comprehensive testing program
humidifiers, and autoclaves. which will analyze your feed water to ensure optimum
Reverse osmosis is effective RO performance.
As the feed water passes through the RO membrane,

impurities deposit on the surface of the RO membrane and
Osmosis Reverse Osmosis

The natural osmosic flow of water through a semi-permeable membrane from less
concentrated solutions to more concentrated solutions. Reverse osmosis is achieved
when external pressure is applied to reverse this natural flow, as shown on the right.
80
RO Systems
Our technical service experts are a great
resource for any feed water questions you
might have.
Here is an explanation of key characteristics impacting

RO performance:
Temperature
Volumes listed for the reverse osmosis product water are
based on a feed water temperature of 15 °C (59 °F). For
every 1 °C below 15 °C (59 °F), the quantity of product
water is reduced by 3 %. Additionally, if the feed water
temperature goes beyond 25 °C (77 °F), the RO membrane
may be damaged. We recommend a hot and cold water
mixing valve to regulate the temperature to 15 °C (59 °F).
Alkalinity and calcium
Carbonates, bi-carbonates, hydroxides and calcium
impurities in your feed water contribute to RO membrane
scaling. Both the Thermo Scientific Barnstead Pacific RO
and Thermo Scientific Barnstead LabTower RO systems
have pretreatment solutions to prevent this. Included in
the pretreatment system is a hardness stabilizer cartridge
which binds calcium, thereby protecting the RO membrane.
Chlorine
Chlorine can damage the RO membrane and reduce its
performance and useful life. Again, both Pacific RO and
LabTower RO have solutions to pretreat the water, which Barnstead LabTower RO with optional hand dispenser
includes a 5 µm prefilter + activated carbon cartridge to
prevent entry of particles >5 µm and high concentrations SDI (Silt Density Index)
of free chlorine.
Suspended solids and colloidal materials in feed
Turbidity water are one of the biggest problems in reverse
The turbidity level indicates the amount of suspended osmosis systems. In order to have some measure
solids present. This suspended material can shorten the life of the degree of this fouling problem, a concept
of filters and reverse osmosis membranes if not removed. called Silt Density Index is used. Here a 0.45 µm
Our Pure Water Experts can recommend pretreatment filter is exposed to the feed water under pressure
options if turbidity is an issue with your feed water. RO and filtration rates are calculated. A SDI of <5 is
membranes show very little fouling when the feed water considered acceptable for the reverse osmosis
has a turbidity of less than 1 NTU. systems.
Barnsteadreverse osmosis purification system selector guide
Technology / Feature Benefits
Choosing the right system will give you the most value.
Optimal amount of water to use daily
Capacity Take into account any new applications you will introduce in
to sustain reasonable cartridge life
the coming years or new volume demands you may have.
Feed water monitoring Alerts you to fluctuations in feed water quality
Offers a stand-alone configuration for labs with

Features Integrated 100 L Reservoir
little bench space
Pretreatment integrated into system Pretreatment integrated into system
82
RO Systems
Pacific RO LabTower RO
Flexible mounting Integrated

options 100 L Reservoir
7, 12, 20 L/hr 40 L/hr
Standard Standard
— Standard
— Standard
Barnstead LabTower RO water purification system
The Barnstead LabTower RO Converts Tap Water into RO Water and stores it in an integrated 100 L reservoir.
Advanced technology in a mobile design • A built-in pretreatment unit, consisting of a hardness

• An ultra-modern controller provides easy-to-read stabilizer for protection of the reverse osmosis module
system parameters from hardness formers
• Completely drainable integrated 100 L high-purity water • An activated carbon/5 μm combi-cartridge protects the
reservoir has a low-noise pressure booster as a system against free chlorine and particles, ensures the
standard component long service life of downstream purification stages
• Mounted on casters, the compact LabTower RO Compact and mobile design

system is an ideal pure water supply for any laboratory. • Stand-alone design with integrated 100 L tank takes up
Ideally suited as a feed water source for dishwashers, ZERO bench space
autoclaves and general laboratory use • Casters allow for easy relocation
Integrated 100 L reservoir
• 100 L RO water storage in a high-purity polyethylene PE
opaque reservoir
• Adjustable reservoir volume can be programmed for
times of low demand
• Dispense from the reservoir to supply autoclaves,
glassware washers, incubators, etc
Easy-to-operate clear display
• Resistivity/conductivity clearly displayed on large back-lit
control panel that tilts for optimum viewing
• Status of current operating mode clearly indicating
“production”, “stand-by”, or “rinsing” modes
• System parameters are code-protected to prevent
accidental changes to set points
• Developed to fulfill GLP requirements
• Recorded and traceable data can be obtained by print
out via the RS-232 interface and accessory printer
Routine Laboratory Work • Highly qualified and precise measurement of the

conductivity is ensured by the cell constant of 0.16 cm-1
• Supplying autoclaves, Quick Look Comparison LabTower RO 40
water baths, incubators and Pure water production at 15 °C , L/hr 40
glassware washers Withdrawal performance from reservoir
180
• Preparing and diluting buffers at 1.5 bar, L/hr
Retention quota for inorganics, % >98
and reagents
Bacteria content, % >99
• General biotechnology
Retention quota for particles, % >99
84
RO Systems
RO water delivered from

an integrated system
Source Potable tap water that has been softened or hardness stabilized
<5. With higher values a pretreatment (model no. 09.4000)
Silt density index (SDI)
must be installed upstream of the system
pH-Range 4-11
LabTower RO Product Specifications

Operating Electrical Power Feed water Dimensions
Temperature
pressure requirements consumption connector WxDxH
29-87 psi 90-240 V, 17.7 x 22.8 x 59 in
0.25 kW 3/4” NPT 2-35 °C
(2-6 bar) 50/60 Hz (450 x 580 x 1500 mm)
LabTower
System Options
RO 40
LabTower RO Systems*
50132391
All systems include an RO membrane(s),integrated 100 L reservoir, and pressure regulator
5 µm filter with activated carbon and a
Pretreatment cartridges 50134022
10 inch filter housing
Both are required for complete pretreatment solution
1 µm filter with a 10 inch filter housing 09.4003
Sterile vent filter for reservoir 50135142
Sterile overflow for reservoir 50132714
Printer Utilizes 120 V, 50/60 Hz STARA-106
RS-232 interface for safe documentation of all
measured values and faults with date and time in
compliance with GLP guidelines 230 V, 50 Hz 09.2207
Water watcher
16.0129
Alerts the user to leaks. Available as 240 V only.
Hand Dispenser Kit
50138221
Hand dispenser with 3 meter cord that connects to tank. Ships with a 0.2 micron final filter.
Reverse Osmosis membrane 22.0087
LabTower TII 40 requires two membranes (must order 2)
10" 5 µm filter with hardness stabilizer cartridge 06.5204
1 μm filter prefilter for 09.4003 06.5101
5 µm prefilter + activated carbon 10 inch cartridge 06.5201
*
Barnstead Pacific RO water purification systems
The Barnstead Pacific RO system is a modularly designed laboratory reverse osmosis system specifically
developed for functional and economical production of high-purity water.
Product Features Reliable operation

• Five system options with permeate flows of 7, 12, • Microprocessor control offers automatic operation with
or 20 L/h continual monitoring of all important parameters
• Space-saving design allows system to sit on the Easy-to-operate clear display
laboratory bench or be mounted on the wall • Resistivity/conductivity clearly displayed on large back-lit
• RO membrane removes organic and inorganic control panel that tilts for optimum viewing
contaminants, microorganisms, particles and colloids • Status of current operating mode clearly indicates
“production”, “stand-by”, or “rinsing” modes
• Good quality feed water is a prerequisite for high purity
water and extends cartridge service life
• Real-time clock records system errors and faults
• Ongoing data storage collects four weeks information
displayed in clear text
• Operating system is code-protected to prevent
unauthorized changes to system settings
• RS-232 interface with adjustable sender interval allows
for customized timing of data transmission of measured
values and faults to a PC or data printer
• Cell constant of 0.16 cm-1 ensures precise
conductivity measurements
Pacific RO pretreatment
• Two 10 inch (25.4 cm) cartridge filter housings with a
5 µm prefilter + activated carbon to prevent entry of
particles >5 µm and high concentrations of free chlorine.
Also includes hardness stabilizer cartridge which binds
calcium and prevents its precipitation.
Worry-free pure water storage
• Reservoir are constructed of pigment-free polyethylene
• Rinsing lab glassware • Conical bottom allows for easy cleaning, disinfecting and
• Supplying autoclaves, water complete emptying
baths and incubators
• Preparing and diluting buffers
and reagents
• General biotechnology
86
RO Systems
Flexible configurations

for your RO water needs
Pacific RO Pacific RO Pacific RO
7 L/hr 12 L/hr 20 L/hr
systems systems systems
Pure water production at 15 °C, L/h 7 12 20
Salt retention, % 98 98 98
Retention, bacteria and particles, % 99 99 99

Source Potable tap water that has been softened or hardness stabilized
<5, With higher values, a pretreatment
Silt density index (SDI)
(model no. 09.4000 must be installed upstream of the system)
Prefiltration 5 µm + activated carbon
pH-range 4-11
Temperature, C° 2-35
Barnstead Pacific RO water purification systems (continued)
Storage Reservoir
Volume Material Dimensions
30 L 23.5 x 14.9 in (598 x 380 mm)
60 L Polyethylene, opaque to light 35.9 x 14.9 in (912 x 380 mm)
100 L 49.2 x 14.9 in (1249 x 380 mm)
Pacific RO Product Specifications

Operating
pressure, Temperature
requirements consumption connector WxDxH
psi (bar)
29-87 90-240 V, 14.6 x 13 x 23.7 in
0.1 kW 3/4” NPT 2-35 °C
(2-6) 50/60 Hz (372 x 330 x 603 mm)
Pacific RO Pacific RO Pacific RO

Ordering Table
7 12 20
Pacific RO Systems*
All systems have an integrated wall bracket and include an 50132386 50132387 50132388
RO membrane
Pacific RO Pretreatment
Includes two 10 inch (25.4 cm) cartridge housings,
09.4000 09.4000 09.4000
a 5 µm prefilter + activated carbon cartridge and hardness
stabilizer cartridge
Pacific RO Reservoir
See "Storage Reservoir Options" the next page
Choose a reservoir that fits your needs
Water watcher
16.0129 16.0129 16.0129
Alerts the user to leaks. Available as 230 V, 50 Hz only.
Reverse Osmosis membrane
22.0046
Pacific RO 7, 12 only require a single RO membrane. 22.0046 22.0046
(order 2)
Pacific RO 20 requires two membranes as indicated.
5 µm prefilter + activated carbon 10 inch cartridge 06.5201 06.5201 06.5201
Hardness stabilizing 10 inch cartridge 06.5452 06.5452 06.5452
*
88
RO Systems
Storage Reservoir Options for Pacific RO 30 L 60 L 100 L
Reservoir with level display 50155495 50155500 50155503

Sterile overflow for reservoir 06.5001 06.5001 06.5001
Vent filter Sterile filter, 0.2 µm 50135142 50135142 50135142
Wall mounting bracket 06.5015 06.5016 na
Pretreatment
Not all feed water sources are equal!
Pretreatment is sometimes necessary to maximize
the effectiveness of water purification systems.
Poor water quality can also shorten the life of water
system consumables.
90
Mixed Multi Water Softener
• Ideal system to treat hard feed water

• Not only softens the water, but also protects the RO
membrane from blockage
• Removes calcium, magnesium, iron, and manganese and
a great variety of suspended matter and organic material
• Combination of multiple stages, from porous polymeric

adsorbers to ion exchangers
Mixed Multi
Pretreatment
• Necessary for feed water with a silt density index (SDI) Water Softener
that is greater than 3 or the total ionized solids (TIS) is
greater than 250 ppm
Single Pretreatment Cartridge

• Easy-to-use pretreatment cartridge protects the
RO membrane in a system against particles
• Ships with 10-inch filter housing and a 1 µm filter
• Ideal when feed water Silt Density Index (SDI) is 2-3
Double Pretreatment System

• Ships with two 10-inch filter housings, a combi-cartridge
which contains a 5 µm filter and activated carbon, and a
hardness stabilizer cartridge
• Removes chlorine, organics, and hard ions
Triple Pretreatment System

• Ships with three 10 inch filter housings a combi-cartridge
which contains a 5 µm filter and activated carbon, and a
hardness stabilizer cartridge, and a 1 µm filter
• An effective triple pretreatment system removes chlorine,
organics, hard ions, and particles larger than 1 µm
Not sure what you need?

Request a complimentary H2O Select
analysis kit and let us analyze your
feed water. Your report will include a
recommendation of any pretreatment
system needed for your water
purification system.
pretreatment selector guide
Use pretreatment when your feed water quality doesn’t match the requirements for your system.
Model
Water Type Solutions Voltage Descriptions
Number
DI 1500 cartridge
Required only when feed water does not meet feed water
RO Water requirements. Prolongs system cartridge life. For new stainless steel cartridge > 02.1500
installations, 04.1690 is a required hose kit to adapt
cartridge to system.
Deionized
Deionized and distilled water will meet the feed water requirements for the Thermo Scientific GenPure and MicroPure
Distilled
families. No pretreatment is typcially required.
Water
Pacific TII and reservoir or LabTower TII see pages
Utilize a Type 2 system to pretreat the GenPure and MicroPure families. 56-63
Pretreatment Filter, 1 µm filter 10 inch 1 µm filter with housing > 09.4003
Tap Water Mix Multi Mini Water softener
The softener is required for most customers who have unsoftened
Mixed multi softener > 50130297
tap water, or a TIS of more than 10 ppm. Additionally, you will need to
5 µm filter with activated carbon > 06.5201
replace the hardness stabilizer cartridge that ships with the unit with a
5 µm filter with activated carbon 230 V, 50 Hz.
Pretreatment Filter, 1 µm filter

Ships with one 10 inch filter housing
It is ideal to place largest pore filter before smaller, so put the 5 µm
10 inch 1 µm filter with housing > 09.4003
filter with hardness stabilizer (ships with the unit) before the 1 µm filter
for optimal performance. Designed for customers without chlorine in
their tap water – typically Europe and Asia Pacific
Pretreatment Filter with carbon

5 µm filter with activated carbon, 1 μm filter
5 µm filter with carbon > 50134022
Ships with two 10 inch filter housings
10 inch 1 µm filter with housing > 09.4003
Designed for customers with chlorine in their tap water – typically
North America, Latin America and UK
Tap Water Single Pretreatment System
5 µm filter with hardness stabilizer cartridge
Ships with one 10 inch filter housing 5 µm filter with hardness stabilizer > 09.4001
Great option for customers with little/no chlorine in their tap water –
typically Europe and Asia Pacific
5 µm filter with activated carbon cartridge, hardness stabilizer
5 µm filter with carbon and a
cartridge. Ships with two 10 inch filter housings 09.4000
hardness stabilizer cartridge >
Designed for customers with chlorine in their tap water – typically
North America, Latin America and UK
Triple Pretreatment System 5 µm filter with carbon and a
5 µm filter with activated carbon cartridge, hardness stabilizer hardness stabilizer cartridge > 09.4000
cartridge, 1 µm filter. Ships with two 10 inch filter housings 1um filter > 09.4003
Mix Multi Mini Water softener

The softener will remove hardness and protect the RO membrane.
5 µm filter with activated carbon > 50134022
Additionally, you will need to add a 5 µm filter with activated carbon
1 µm filter > 09.4003
and a 1 µm filter. 230 V, 50 Hz. Ships with two 10 inch filter housings
Hard
Tap Water Mix Multi Mini Water softener
The softener will remove hardness to protect the RO membrane.
Additionally, you will need to replace the hardness stabilizer cartridge
5 µm filter with activated carbon > 06.5201
that ships with the unit with a 5 µm filter with activated carbon. 230 V,
50 Hz.
* Ships with 5 µm filter with hardness stabilizer.
92
Type 1 TAP Type 1 Type 2 RO

LabTower LabTower LabTower
Pretreatment
GenPure MicroPure Smart2Pure Pacific TII Pacific RO
EDI* TII* RO*
3 3
3 3
3
3 3
3 3
3 3
3 3
3 3
3 3
3 3
94
Pretreatment selector guide (continued)

Pretreatment Component
Pretreatment Description Model Component Description(s) Model
Number(s) Numbers
10 in (25.4 cm) Activated carbon cartridge with
Single Pretreatment Carbon 50134022 06.5201
5 μm filter
Single Pretreatment System -
09.4001 10 in (25.4 cm) 5 µm filter and hardness stabilizer 06.5204

Hardness Stabilizer
Single Pretreatment System -
09.4003 10 in (25.4 cm) 1 µm filter 06.5101
Pretreatment
Prefilter
10 in (25.4 cm) Activated carbon cartridge with 5 µm filter 06.5201
Double Pretreament System 09.4000
10 in (25.4 cm) Hardness stabilizing cartridge 06.5452
10 in (25.4 cm) 5 µm filter with activated carbon cartridge 06.5201
09.4000
Triple Pretreatment System 10 in (25.4 cm) Hardness stabilizing cartridge 06.5452
09.4003
10 in (25.4 cm) 1 µm filter 06.5101
Salt, 25 kg 50155490
Mix Multi Mini, 230 V, 50 Hz 50130297 Hardness measurement kit 06.1000
5 µm filter with carbon 06.5201
Mix Multi Mini, 230 V, 50 Hz

Max flow rate, L/h 500
Filter volume, L 5
Capacity, m³ at 10 °dH 2
Salt storage, kg 20
Operating pressure, psi (bar) 20-87 (1.4-6)
Water temperature max, °C 40
Controller time controlled
Dimensions, W x D x H, in (mm) 9 x 17.7 x 22 (230 x 450 x 560)
Model number 50130297
What is SDI? • SDI is a measure of the fouling capacity of water in

RO systems
SDI stands for silt
density index (SDI). • The test measures the rate at which a 0.45 µm filter is
When the SDI of feed plugged when subjected to a constant water pressure
water is >3, it can of 30 psi
quickly ruin your • SDI gives the percent drop per minute in the flow rate
RO membrane of the water through the filter, averaged over a period
of time such as 15 minutes
Cartridges and
filter systems
We offer a complete line of cartridge and
filter systems for all your single step
purification and pretreatment needs. Use the
Bantam Deionizer, Hose Nipple Cartridges
or B-Pure cartridges for simple, single step
purifications.
96
Cartridges
Innovation

Joints and seams are ultrasonically
welded to prevent contamination
Filters Systems
from chemical adhesives
Convenience
Quick-release designs provide easy
cartridge change-outs and simplify
maintenance, bypassing the costs
of service calls
Flexibility
Versatile configurations meet an
array of specific applications
Are the water systems you’re
purifying under pressure?
Barnstead cartridge & filter selector guide
Choose the
feature you need Technology / Feature Benefits
Digital purity indicator features real-time readout of resistivity

Digital Purity Indicator
values; useful with more sensitive applications
Quick glance monitoring to notify user when it’s time to install

Pura-Lite “Go/No Go” / a new cartridge. Useful if cartridge system feeds a piece of lab
Purity Indicators
Analogue Resistivity Indicator equipment, such as an autoclave, where real time resistivity is
not required.
Color change indicates when the cartridge is beyond its useful

Color Change – Cartridge
life and must be replaced. Useful for general lab applications.
Hand dispense water from the cartridge system. Ideal for rinsing
Remote Dispenser
glassware or filling a waterbath.
Saves laboratory bench space. Ideal configuration for autoclaves

Wall Mounted
and dishwashers where no bench space exists.
Added Features
Quick Release Canisters Allows for fast and simple cartridge change-outs.
Maximum Flow Rate L/min Enhances productivity
98
Cartridges
Non-Pressurized
Pressurized Systems Water Systems

Filters Systems
B-Pure Economical
B-pure B-pure Bantam
Half Size Harvey DI+ Filter Hose Nipple
Double Holder Single Holder Deionizer
Holder Holder
Expanded Complete Economical Simple
Smaller Complete
purification Single step system choice for system for
capacity for nonpressurized
with full purification with dispenser particle nonpressurized
optimal value system
features and cartridge filtration applications
3 3 3 3 3
Requires
3 3 3 3 hard piping to 3 3
support unit
3 3 3 3
4 4 2 4 2 0.75 0.63
B-Pure water system
The B-Pure is an economical laboratory grade water system with flow rates up to 4 L/min that can be customized
to meet your specific applications. Choose from single or double holders depending on your application needs.
• Customize your water system to meet your application

needs. Modular design provides the ability to add
additional holders when the need arises
• Produces up to four L/min
• Quarter turn quick release canisters make changing
cartridges simple
• Accepts Thermo Scientific Barnstead Pura-Lite purity
indicator or digital purity meter to monitor water quality
• Available in full or half-size cartridge configurations
supporting space constraints
Single holder
• Simple system without a draw-off valve
or purity indicator
Double holder
• Complete with draw-off valve
• Choose from models with digital purity meter
or Pura-Lite “Go/No Go” resistivity indicator
Half-size holder
• Produces up to 2 L/min
• Easily attaches to full size B-Pure systems
• Small size allows for flexibility in mounting location

Resistivity reading Flow rate
1-10 MΩ.cm
Up to 4 L/min
(depending on cartridges used)
Applications Include
• Pretreatment
• Deionization
• Organic and chlorine removal
• Feeding laboratory equipment
100
Cartridges
Customizeable for your

pretreatment or deionization needs
Filters Systems
Thermo Scientific Harvey DI+ Cartridge
System
• Turn-key system ships complete with a remote
dispenser, Pura-Lite (50 KΩ.cm) indicator and a high
capacity, two-bed ion exchange cartridge to remove
calcium, magnesium, and other ions from water
• The system accepts a broad range of other cartridges
to remove impurities and produces pure water at a flow
rate of up to 4 L/min
Overall dimensions Inlet Feed water Max feed Max flow
WxHxD connection temperature water pressure rate
7 x 24 x 7 in
Single holder 1/2 in NPTF 4-49 °C (40-120 °F) 100 psig 4 L/min
(17.8 x 61 x 17.8 cm)
15 x 27 x 7 in
Double holder 1/2 in NPTF 4-49 °C (40-120 °F) 100 psig 4 L/min
(38.1 x 68.65 x 17.8 cm)
7 x 15 x 7 in
Half size holder 1/2 in NPTF 4-49 °C (40-120 °F) 100 psig 2 L/min
(17.8 x 38.1 x 17.8 cm)
7 x 26 x 7 in
Harvey DI+ 1/2 NPTF 4-49 °C (40-120 °F) 100 psig 4 L/min
(17.8 x 63 x 17.8 cm)
B-Pure water system (continued)
Cartridges
Size Dimensions Length x Diameter
Full Size Cartridge 17.5 x 3.4 in
Half Size Cartridge 10.95 x 3.4 in
S ystem Options Electrical Model number

Single holder – D4511
120 VAC D4521
Double holder with digital purity meter
240 VAC D4522-33
Double holder with Pura-Lite indicator (50 kΩ) 120 VAC D4524
Double holder with Pura-Lite indicator (200 kΩ) 120 VAC D5831
Double holder with Pura-Lite indicator (1 MΩ) 120 VAC D5833
Half size holder – D4505
Harvey DI+ 120 V AY1273X4
102
Choose the cartridge(s) according to Resin Cartridge Capacity as Model
Cartridges
your needs. type(s) size CaCO 3 number
Anion removal
Effective removal of weakly ionized impurities, Strong anion
Full 1680 grains D0760
raises the pH of solutions, recovers precious exchange
metal complex
Cation removal
Converts ionized salts to the acid form resulting Strong cation
in a product water that is low in pH, ideal for exchange

precious metal or isotope recovery
High capacity
Removes ionized impurities, produces a larger Two-bed ion
Filters Systems
quantity of water than that of the Ultrapure exchange
(D0809), however at a lower resistivity
Pretreatment macropure
Macroreticular,
Effectively removes colloids and bacteria, Full 2000 gal D0836
activated carbon
increases filter life
Organic removal Full 5000 gal D0813
Activated carbon
Removes organics and chlorine Half 5000 gal D50215
Organic removal
Removes organics and chlorine. Ideal for
Extruded carbon Full 5000 gal D63112
pretreating water systems with high organic levels
in the feed water.
Oxygen removal Full 30 g D0811
Maintains low oxygen content to prevent corrosion Porous strong
in cooling water loops, etc. The feed water should anion exchange
contain less than 10 ppm of ionized solids. Half 18 g D50214
Oxygen removal 275 grains /
Oxygen and demineralization Half D50216
mixed-bed 9g
Ultrapure Full 875 grains D0809
Removes ionized impurities to produce high Mixed-bed
resistivity water with a neutral pH Half 550 grains D50213
Ultrapure and organic removal 785 grains /
Full D0832
Commonly used as still pretreatment. Removes Mixed-bed, 1000 gal
ionized impurities and has alayer of activated activated carbon 275 grains /
carbon to remove chlorine and organics. Half D50217
2000 gal
Ultrapure, 455 grains /
Ultrapure and oxygen removal Full D8809
oxygen removal 30 g
Ultrapure, 365 grains /
Ultrapure, oxygen, and organic removal oxygen and Full 2000 gal / D8811
organic removal 12 g
Voltage
120 240
Digital Purity Monitor – Resistivity range of 0.1-18.2 MΩ.cm D2770 D2769
Indicator light at 50,000 Ω.cm E3450 E3454
Pura-Lite Indicator light at 200,000 Ω.cm E3451 E3455
Indicator light at 1,000,000 Ω.cm E3452 E3456
1 to 75 L/hr using 1/8 inch NPT inlet D0787 D0787
Flow meters
0 to 190 L/hr using 1/8 inch NPT inlet D0788 D0788
0.2 µm final filter – For use on Double Holder units D3750 D3750
Bantam Deionizer
The Barnstead Deionizer is an economical way to deionize your water. Easily view resistivity readings directly
from unit. Customizable to specific applications by selecting the appropriate cartridge listed below.
Simple deionization Quick Look Comparison

Resistivity reading
25,000-18,000,000 Ω-cm
Flow rate
Up to 38 L/hr
system System Options

Overall Feed
Model
• Ideal for applications requiring up to 38 L/hr of dimensions water Electrical
number
WxHxD pressure
purified water
6 x 28.5 x 120 VAC D0800
• Select the cartridge to best meet your application needs Bantam 8.75 in
5-70 psi
Deionizer* (15.2 x 72.4 x
• Point of use polishing of pretreated water or single stage 22.2 cm) 240 VAC D0805
treatment of tap water
• Direct reading purity monitor Required Accessories
• Designed for non-pressurized applications. Output Choose the cartridge based Resin Model
Capacity
on your application needs Type(s) Number
water must be diverted to an atmospherically-vented
High capacity
receptacle, no back pressure can be accepted Removes ionized impurities,
Two-Bed
• Minimum inlet pressure 5 psi, maximum inlet pressure 70 psi produces a larger quantity of 1760
Ion D0803
water than that of the grains
Exchange
• Includes 3 ft (0.9 m) of inlet feed tubing Ultrapure (D0809), however
at a lower resistivity
Ultrapure
Removes ionized impurities Mixed- 875
D0809
to produce highest resistivity Bed grains
water with a neutral pH
Ultrapure and organic
removal (pretreatment) Mixed-
785
Removes ionized impurities Bed,
grains / D0832
and has a layer of activated Activated
1000 gal
carbon to remove chlorine and Carbon
organics

• Pretreatment
• Deionization
• Organic and chlorine removal
104
Barnstead Hose Nipple Cartridges
Cartridges
The Barnstead Hose Nipple Cartridges are an economical way to purify water volumes up to 75 L/hr
Applications include softening, deionization, organic and chlorine removal.

Easily customizable
• Economical purification option
Filters Systems
• Ideal for small volume applications
cartridge holder
• Same superior quality resins used in
B-Pure cartridges
• Constructed of 100 % virgin
polypropylene cartridge
• Designed for non-pressure applications, these cartridges Cartridge size Dimensions (Length x Diameter)
include 3/8 inch barb hose connections on each end. 1/2 Size 10.2 x 3.25 in (25.9 x 8.25 cm)
D8822, D8950, and D8951 cartridges include tapered 2/3 Size 13.3 x 3.25 in (33.8 x 8.25 cm)
Full Size 18.7 x 3.25 in (47.5 x 8.25 cm)
straight nipple for 3/8 inch ID tubing
• Color change indicator identifies when
resin is exhausted in specific cartridges System Options Model
number
• Simple cartridge holder is easily Hose nipple cartridge holder
D8900
wall mounted For easy wall mounting
Choose the
Color Model
cartridge based Resin type(s) Capacity
indicator1 number
on your needs
1/2 size mixed-bed Ultrapure ion exchange 430 grains No D50220
1/2 size Ultrapure mixed-bed
280 grains /
mixed-bed with with strong anion No D8822
4.4 g
oxygen removal exchange
Two-bed ion
2/3 size high
exchange with strong 1100 grains Yes D8950
capacity
cation exchange
2/3 size Mixed-bed ion
470 grains /
mixed-bed and exchange with Yes D8951
2000 gal
organic removal activated carbon
Full size cation
Cation exchange 3000 grains Yes D8905
removal
Full size high Two-bed ion
1650 grains Yes D8901
capacity exchange
Full size
Macroreticular
macroreticular, n/a No D8908
cation exchange
cation removal
Full size organic
Activated carbon 5000 gal No D8904
removal
Full size oxygen Strong anion
30 g No D8903
removal exchange
Routine Laboratory Work Full size
Mixed-bed and
strong cation 1250 grains
pretreatment and Yes D8921
• Pretreatment exchange, activated / 1000 gal
scale eliminator
charcoal
• Deionization Full size ultrapure Ultrapure ion exchange 915 grains No D8911
• Organic and chlorine remova Ultrapure mixed-bed
Full size ultrapure 915 grains Yes D8902
ion exchange
• Feeding laboratory equipment Full size ultrapure Mixed-bed ion
730 grains /
with organic exchange with Yes D8922
2000 gal
removal activated carbon
1
Alcohol-containing water supplies cannot be used with cartridges containing the color indicator.
Barnstead 1/2 Size B-Pure Filter
Barnstead 1/2 Size B-Pure Filters will meet all your prefiltration needs. The filters can be used to pretreat feed
water or act as a final filter to ensure consistently pure water.
Product features
• Large surface area ensures long life
• Flow rates of up to two L/min
Choose from two holder options

1/2 Size B-Pure Filter Holder
• Natural polypropylene material construction
maintains purity
• Filters are easily and quickly replaced with quarter turn
quick release canisters
• Accepts 10 inch (25.43 cm) pre- and post-filters
• Wall mounting bracket comes standard
Economy Filter Holder
• Accepts 10 inch (25.43 cm) double open-ended filters
• Can be piped into any water line
Optional accessory
Pressure Gauge Kit
• Attaches to any B-Pure system
• 0 to 160 psi pressure gauge allows for monitoring inlet
and outlet water pressure
• Monitors pressure drop across any of the 10 inch
Economy Filter Holder
(25.43 cm) filter cartridges used in the 1/2 Size B-Pure
Filter Holder
• Pressure drop of greater than 10 psi indicates need for
filter change

• Particulate and bacteria removal
106
Cartridges
Flexible

filtration system
Filters Systems
System Options Product Specifications
Max feed
Overall dimensions Inlet Feed water Max flow Model
water
WxHxD connection temperature rate number
pressure
1/2 Size B-Pure
7 x 15 x 7 in 4-49 °C
holder* 1/2 in NPTF 100 psig 2 L/min D5839
(17.8 x 38.1 x 17.8 cm) (40-120 °F)
Wall mount included
Economy filter 5.25 x 12 x 5.25 in 4-49 °C
3/4 in NPTF 100 psig 2 L/min 15840
holder* (13.3 x 30.5 x 13.3 cm) (40-120 °F)
Required Accessory
1/2 size
Length Diameter Economy
Pore Size B-Pure
in (cm) in (cm) Holder
Filter Holder
1 µm Pre-filter 10 (25.4) 2.5 (6.5) FL583X4 18024
5 µm Pre-filter 10 (25.4) 2.5 (6.5) FL583X1 D2729
Filters 10 µm Pre-filter 10 (25.4) 2.5 (6.5) FL583X2 18011
Choose the filter(s)
according to your needs 15 µm Pre-filter 10 (25.4) 2.5 (6.5) FL583X3 18018
0.2 µm Final Filter 10 (25.4) 2.5 (6.5) FL583X6 –
0.45 µm Final Filter 10 (25.4) 2.5 (6.5) FL583X5 –
Dual pressure gauge kit D2780 –
Single pressure gauge D0780 –
*
Already have a Thermo Scientific water purification system?
This is where you can find the replacement consumables for your previously purchased
Thermo Scientific water purification system.
Regularly changing filters and

cartridges protects your water quality
Water purification systems can only produce pure water
when the cartridges and filters are changed according
to the operational manuals. Regular maintenance of
your water system will ensure the product water is
consistently the highest purity.
Cleaning your
purification system
allows bacterial
contaminants to be
flushed away
Changing the UV lamp protects the

system against microorganisms and
oxidizes organic compounds in the
feed water, allowing for TOC levels of
less than 5ppb
Barnstead NanoPure System Consumables

NanoPure
NanoPure NanoPure NanoPure
Life Science
Analytical Analytical UV Biological UF
UV/UF
Standard unit D11901 D11911 D11921 D11931
+ TOC monitor D11941 D11951 D11961 D11971
Diamond cartridge pack
Choose the cartridge pack Deionized feed D50283 D50281 D50283 D50281
according to your feed water and
system. Ships with 0.2 μm final RO or
D50282 D50280 D50282 D50280
filter (D3750) distilled feed
0.2 μm final filter D3750 D3750 D3750 D3750
Ultraviolet lamp (system) n/a LMX13 n/a LMX13
Ultrafilter n/a n/a FL1192X1 FL1192X1
Cleaning syringe CMX25 CMX25 CMX25 CMX25
Ultraviolet lamp (TOC) LMX26 LMX26 LMX26 LMX26
108
Barnstead EasyPure II System Consumables
EasyPure EasyPure EasyPure EasyPure

II II UV UF II UV/UF
D7381 D7401 D7411 D8611

Legacy Consumables
Cartridge pack Deionized feed D502126 D502124 D502126 D502124
Choose the cartridge
pack according to your RO or
feed water and system D502127 D502125 D502127 D502125
distilled feed
0.2 μm final filter D3750 D3750 D3750 D3750
Ultraviolet lamp n/a LMX13 n/a LMX13
Ultrafilter n/a n/a FL1192X1 FL1192X1
Cleaning syringe CMX25 CMX25 CMX25 CMX25
Barnstead EasyPure RF System Consumables Barnstead EasyPure RoDi System Consumables
EasyPure EasyPure
EasyPure RoDi
RF RF UV
D7031 D7035 D13321
Replacement Consumables Replacement Consumables

Cartridge pack Deionized Cartridge kit
Choose the D502126 D502124 Includes pre-filter, ultrapure mixed
feed D502133
cartridge pack bed cartridge, and EasyPure high
according to your RO or purity/low TOC cartridge
feed water and distilled D502127 D502125 0.2 μm final filter D3750
system feed Ultraviolet lamp LMX13
0.2 μm final filter D3750 D3750 Ventgard reservoir cap CV742X5A
Ultraviolet lamp n/a LMX13 Reverse osmosis membrane FL1332X2
Ventgard reservoir cap CV703X4A CV703X4A Set of three empty cartridges
Set of three empty cartridges D7034
D7034 D7034 for cleaning
for cleaning
Barnstead TII System Consumables Barnstead RO System Consumables
Barnstead TII Barnstead RO
RO 6 L/h D12671
TII 12 L/h D14031
RO 12 L/h D12651
TII 24 L/h D14041 RO 24 L/h D12661
Replacement Consumables Replacement Consumables
TII DI cartridge pack 6 and 12 L/h FL1265X1
D502137 Reverse
Ships with 0.2 μm final filter (D3750) 24 L/h
osmosis FL1265X1
1 μm pre-filter D502113 Requires 2 RO
membrane (please order 2)
MPS cartridge D502114 membranes
Extruded carbon cartridge D502115 1 μm pre-filter FL1265X1
0.2 μm final filter D3750 MPS cartridge D502114
Reverse osmosis membrane FL1265X1 Extruded carbon cartridge D502115
Ultraviolet lamp (system) LMX13 Ventgard reservoir cap 25001-DB
Ultraviolet lamp (reservoir) LMX31
Ventgard reservoir cap 25001-DB
Support and
maintenance
Contact the Lab Water Experts

Have a question or concern? Contact our Pure Water Experts!
Technical Questions Customer Service New Lab Construction

Our technical service team is ready Let our professional and experienced We offer the most comprehensive line
to answer any of your questions on customer service representatives of water purification systems in the
your existing systems. guide you when choosing your water world. We can design a water system
system. They will evaluate your needs, to meet your new lab’s needs.
develop a system recommendation
and coordinate the installation of your
water system.
110

Support and Maintenance
Services
Maintaining your water purification system is crucial to the overall productivity of your laboratory, the
long-term performance of the system and the total cost of ownership. We offer a variety of services to
suit your individual needs. Professional service delivers improved productivity, convenience,
peace-of-mind and budget control.
Preventive maintenance Installation Validation

To ensure your system is working Our factory-trained technicians We offer validation services
within specifications, regular can install your system to ensure it according to cGMP/GLP regulations,
maintenance is essential. We operates with precision. With our certifying that your system is
offer various service options and services, you will receive: performing to pre-determined
preventative maintenance, which will • Installation scheduled at technical specifications. Our
extend the working life, protect your your convenience validation services provide you with:
system and prevent costly breakdown. • Factory-trained experts –
• Assurance that all technical
specifications are met experienced in cGMP/GLP
environments and Good
• Practical hands-on instructions
Document Practice
• IQ/OQ factory-developed
validation protocols
Please ask your local sales representative for additional information about service offerings in your area.
Go Green
Smart Green designs mean a great water system for you and a smaller
impact on the environment!
Our engineers created water systems with the environment in mind:
Reduce
Our cartridges use high quality resin to extend the useful life of the cartridge.
Extended life reduces the need to replace your cartridges which, in turn,
reduces the waste stream and improves your laboratory’s sustainability.
You’ll use your cartridges longer, reducing the need to reorder, ship, handle
and dispose of your cartridges.
Our units are designed to minimize the amount of water that is used during
the analysis.
To provide the customer with pure water and low power consumption, our units
go into a low energy mode after dispensing without sacrificing water quality for
your applications.
Reuse
Our cartridges were engineered with the environment in mind. The plastic
cartridge casings and endcaps are constructed using virgin polypropylene to
allow for easy recycling after end of life. The cartridge is assembled in a clean
environment using environmentally friendly ultrasonic welding that minimizes
energy usage compared to other joining as well as avoids the use of any
environmentally unfriendly solvents. Our conscious effort to avoid chemical
solvents and adhesives makes the plastic and resin suitable to be recovered,
reground and reused for a variety of alternate uses.
112
Common Conversions
Common Conversions for Pure Water
1 U.S. Gallon (gal) = 231 Cubic in (in3) = 3.785 Liters (L)
Volume 1 Cubic Meter (m3) = 35.3 Cubic Feet (ft.3)
1 U.S. Ounce (oz) = 29.57 Milliliters (mL)

1 U.S. Gallon (gal) = 8.33 lb. Water
Density
1 Cubic Foot (ft.3) = 62.3 lb. Water
°F = (°C x 9/5) + 32
Support and Maintenance

Temperature K = °C + 273
°F = (K - 273.15) * 9/5 + 32
1 lb = 453.6 grams (g) = 7000 grains (gr.)
Mass
1 kg = 2.205 lb.
1 Atmosphere = 14.7 Pounds Per Square Inch (psi) = 101.325 Kilo Pascal (KPa)
psia (absolute) = psig (gauge) + 14.7
Pressure 9.92 in Hg = 33.83 ft. H2O
1 kg/cm2 = 14.233 psi
1 KPa = 0.145 psi
1 Grain Per Gallon (gr/gal) = 17.1 Parts Per Million (ppm)
1 Grain Per Gallon as NaCl = 0.85 Grains Per Gallon as CaCO3
1 Part Per Million (ppm) = 1 Milligram Per Liter (mg/L)
Total ionized
1 ppm as NaCl = 0.85 ppm as CaCO3
solids concentration
1 ppm = 1,000 Parts Per Billion (ppb) = 1,000,000 Parts Per Trillion (ppt)
1 ppb = 1 Microgram Per Liter (µg/L)
1 ppm = 1.5 µS/cm
Resistivity/Conductivity 1 megohm-cm = 1/(µS/cm) = 1/( µmho/cm)
Calculating Ion Exchange Capacity

Cartridge Grain Capacity / Total Dissolved Solids (Grains/Gal) = Gallons Processed
Cartridge Grain Capacity x 17.1 / Total Dissolved Solids (ppm) = Gallons Processed
Cartridge Grain Capacity x 64.7 / Total Dissolved Solids (ppm) = Liters Processed
Glossary
Absorption – Process by which one substance is taken Exhaustion – When absorbents, such as activated carbon
up by another, either chemically or physically, as when a or ion exchange resins, have depleted their capacity by
sponge “soaks up” a liquid. using up all active sites. Ion exchange resins may be
regenerated to reverse the process.
Activated Carbon – Material used to adsorb organic
impurities from water. Feed Water – Water entering a treatment process.
Anion Exchange Resin – An ion exchange material that Filtration – Removal of suspended matter by passing
removes anions from solution by exchanging them with it through a porous matrix that prevents particles from
hydroxyl ions. getting through, usually by entrapment on or in the
filter matrix.
Bed – Column of carbon, sand or ion exchange resins
through which the water passes during treatment. Fouling – When gelatinous coatings, colloidal masses
or dense bacterial growth form a compacted crust on
Cation Exchange Resin – Ion exchange resins capable of
membrane or filter surfaces which blocks further flow.
removing cations by exchanging them for hydrogen ions.
Grains per Gallon – Concentration of dissolved ions in
Conductivity – The reciprocal of resistivity, it is a measure
water, generally as calcium carbonate. 7,000 grains is equal
of the ability to conduct an electric current. Since ionized
to a pound. One grain per gallon is equivalent to 17.1 ppm
impurities increase the conductivity of water, it is also an
of Dissolved Solids.
accurate measure of ionic purity. To measure it, current
is passed between two electrodes a fixed distance apart. Hardness – Concentration of calcium and magnesium
Conductivity is normally expressed as microsiemens/cm, salts in water and is sometimes also taken to include iron
identical to micromhos/cm. and manganese. High hardness can cause boiler or pipe
scale and failure of reverse osmosis membranes.
Deionization – Removing dissolved ions from solution by
passing it through a bed of ion exchange resins, consisting Ion Exchange – Also called deionization. A process
of polymer beads that exchange hydrogen ions for cations in which harmless ions attached to the resin beads are
and hydroxyl ions for anions in solution. The ionic impurities exchanged for undesirable ions in solution. Typically,
remain bound to the resins, and the hydrogen and hydroxyl hydrogen ions are exchanged for any cations and hydroxyl
ions combine with each other to form water. ions for any anions. The hydrogen and hydroxyl ions
combine to form pure water.
Dissolved Solids – Also referred to as Total Dissolved
Solids (TDS), it is the amount of nonvolatile matter Macroreticular Resin – An ion exchange resin with a
dissolved in a water sample and is usually expressed in reticular porous matrix that makes it effective for removing
parts-per-million by weight. colloids and bacteria, as well as dissolved anions. It is
especially useful for preventing colloidal and organic fouling
Distillation – The process of separating water from
of mixed-bed resins and premature clogging of final filters.
impurities by heating until it changes into vapor and then
cooling the vapor to condense it into purified water. Megohm-cm – Ω A measure of ionic purity in water.
Resistivity (the reciprocal of conductivity) is a measure of
Electrodeionization (EDI) – Removes dissolved ions
specific resistance to electrical flow. The fewer dissolved
from solution using electricity to ionize water and
ions in water the higher its resistivity. One megohm-cm
separate ions.
is equivalent to one million ohms of resistance measured
Effluent – The output or discharge from a water between two electrodes one centimeter apart.
treatment process. The theoretical maximum ionic purity for water is
18.2 megohm-cm at 25 °C .
Endotoxin – A poisonous substance present in bacteria
that is released when the cell disintegrates. In water
treatment, it most often refers to pyrogens.
114

Membrane – Filtration membranes are thin polymer films
that are permeable to water and other fluids. Microporous

membrane filters have measurable pore structures which
physically remove particles or microorganisms larger than
Glossary
pore size. Ultrafiltration membranes (sometimes called
molecular sieves), also remove molecules larger than a
specified molecular weight. Reverse osmosis membranes
are permeable to water molecules and very little else,
rejecting even dissolved ions in water.
Mho – Unit of measurement for conductance; the

reciprocal of ohm (resistance). See Megohm-cm.
Mixed-Bed Ion Exchange – Mixing both anion and cation

resins in the same deionizer results in higher efficiency, but
lower capacity than separate-bed deionizers.
NPT – Abbreviation for National Pipe Thread.
Ohm – The practical unit of electrical resistance in a circuit,

where a potential difference of one volt produces a current
of one ampere.
Ohm-cm – Unit of specific resistance of water.
Osmosis – The diffusion of a solvent through a

semipermeable membrane from a less concentrated
solution to a more concentrated solution.
Percent Recovery – In reverse osmosis or ultrafiltration,

the ratio of pure water output to feed water input.
Percent Rejection – In reverse osmosis or ultrafiltration,

the ratio of impurities removed to total impurities in the
incoming feed water. For example, RO membranes
typically remove (reject) 90 % of the dissolved inorganic
contaminants in water.
Permeate – In reverse osmosis, the water that diffuses

through the membrane, thereby becoming purified water.
Pretreatment – Initial water treatment steps performed

prior to final processing to prolong the life of cartridges
and filters and to protect downstream elements from
premature failure.
Product Water – The purified water produced as a result

of treatment.
Glossary
Pyrogens – Lipopolysaccharides found in the outer Semipermeable – Membranes that do not have
cell walls of certain bacteria which can trigger an measurable pores, but through which smaller molecules
immune response. can pass.
Recirculation – Continuous recirculation may be Suspended Solids – Undissolved solids that can be
necessary to maintain uniformly high purity in larger removed by filtration.
water systems. Water is continuously recirculated and
TDS – Abbreviation for total dissolved solids.
reprocessed to prevent stagnation and to rinse out residual
impurities in the system. Bacteria flourish in stagnant water. Total Ionized Solids – Concentration of dissolved ions
in solution, expressed in concentration units of NaCI
Reject – In reverse osmosis and ultrafiltration, those
(sodium chloride).
impurities not able to permeate the membrane are said to
be rejected (removed). They are flushed away in the reject Total Organic Carbon (TOC) – A measure of the level of
(waste) stream. organic impurities in water, which determines the operating
life of activated carbon beds.
Resin – Ion exchange resins are usually bead-like
spherical materials with an affinity for particular ions. Total Solids – Total solids in water include both dissolved
Cation exchange resins, made of styrene and and suspended solids.
divinylbenzene containing sulfonic acid groups, will
Turbidity – A suspension of fine particles that obscures
exchange hydrogen ions for any cations they encounter.
light rays but requires many days for sedimentation
Similarly, anion exchange resins, made of styrene and
because of small particle size.
divinylbenzene containing quaternary ammonium groups,
will exchange a hydroxyl ion for any anions. Two-Bed – Separate beds or layers of cation and
anion exchange resins. Results in lower purity than
Resistivity – A measure of specific resistance to the flow
mixed-bed deionization, but provides higher capacity in
of electricity. In water, it is an accurate measure of ionic
terms of throughput.
purity. See Megohm-cm.
Ultrafiltration – Molecular sieves; membranes with
Reverse Osmosis – The reversal of osmosis to purify
pores small enough to remove large molecules. Rated in
water. In osmosis, water diffuses through a
terms of nominal molecular weight cutoff. A 10,000 Dalton
semipermeable membrane from a less concentrated
(molecular weight) UF membrane, for example, will remove
solution to a more concentrated solution. The flow of
bacterial pyrogens, which are typically in the range of
water can be reversed with an opposing pressure that
20,000 Daltons.
exceeds osmotic pressure. With Reverse Osmosis, water
is forced out of a concentrated solution, leaving the solute Ultrapure Water – Water with a specific resistance higher
(impurities) behind. than 1 megohm-cm. In the laboratory, it usually refers
to ASTM D1193 Type 1 reagent grade water. Anything in
Salt Rejection – In reverse osmosis, the ratio of salts
laboratory water that is not H2O is an impurity. Although
removed (rejected) to the original salt concentration.
chemically pure water is not attainable, ultrapure water
See Percent Rejection.
systems are now capable of reducing impurities down to
Scale – The mineral deposits that can coat the the limits of detection.
insides of boilers or the surfaces of RO membranes.
UV Oxidation – Ultraviolet radiation is employed in water
It consists mainly of calcium carbonate, which precipitates
purification for the photochemical oxidation of organic
out of solution under certain conditions of pH, alkalinity
impurities, resulting in HPLC grade water with organic
and hardness.
impurity levels below 5 ppb.
116

Glossary
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Smart H2O for you,

and your science

Thermo Scientific Water Purification Products

Common Water Impurities

Suspended Particles Colloids Inorganic Ions Dissolved Organics

Contaminants to Avoid in Your Lab Water

Dissolved Gases Microorganisms Pyrogens and Viruses Nucleases

A basic distillation diagram

Barnstead water purification products offer both depth • Clogging

Ultrafiltration (UF) Benefits

the ion exchange resin beds. Cation resin contains

resistivity requirement for Type 1 reagent grade water.

Two-bed deionization –­ The cation and anion resin are

• Removes dissolved inorganics ions very effectively

Electrodeionization (EDI) retained by the ion exchanger resins and so continually

On applying an electric voltage, water (H20) is split into H+ Limitations

• Long life CI2

• Will not remove ions and particulates

Ultraviolet (UV) Oxidation Combination Ultraviolet Oxidation

What Does Type 1 Water Mean?

ASTM Standards for Reagent Water

The ASTM standards are further subdivided into A, B and C.

ASTM Standards for Reagent Water

Advanced Technology Used to Monitor Purity

Water full of ions Pure water has very

Conductivity Resistivity Conductivity Resistivity

Conductivity and Resistivity

What Type of Water Do You Need?

What Barnstead Water Systems Provide

Ultrapure (Type 1) Water Pure (Type 2) Water

Reverse Osmosis (RO) Water Cartridge and Filter Systems

Barnstead Hose Nipple Barnstead B-Pure Barnstead Bantam

Pretreatment Barnstead Pacific TII 30 L Polyethylene Barnstead GenPure

Pretreatment Barnstead LabTower TII Barnstead GenPure Pro UV

Dual Cartridge Barnstead Mega-P­ure Still with Barnstead MicroPure UV

Pretreatment Barnstead Smart2Pure UV/UF

Ultrapure Water Purification Systems

Select the water system that

UV oxidation is necessary for the removal of organics in the

Cell culture, monoclonal antibody

Nuclease and pyrogen-free applications

Photosensor continually checks the intensity of the

Feed water monitoring alerts you to fluctuations in feed

Real-time monitor of the amount of organic materials in the

Dispense at the push of a button. Control from

RS-232 data printing at

Optimal amount of water to use daily to

* Applicable when using pre-treated feed water only.

Ultrapure Water Purification Systems

Integrated feed water monitoring

Ultrapure Water Purification Systems

Quick Look Comparison

Visible ultrapure water quality

18.2 MΩ.cm: Resistivity TC: temperature

17.5 °C: Temperature measured for

1 ppb: TOC value of

Our Barnstead GenPure xCAD Plus remote

For increased flexibility, add up to two

­Feed Water Requirements*

Two-bed deionization – The cation and anion resin are

Dual Cartridge Barnstead Mega-Pure Still with Barnstead MicroPure UV

Feed Water Requirements*

S ystem Options UV option LabTower TII 40