Fiche ChroNOline 10/23/07 9:57 AM Page 1

CONSUMER TEST ChroNOlineTM

B i o t e c h n o l o g i e
Test performed on 30 consumers for a period of 56 days. The consumers were asked to apply the cream SUMMARY
(2.5% ChroNOlineTM) in the morning and at night.
• A new and innovative anti-aging peptide
The expression is younger 72% • A premium and patented product
The skin around the eye is more even 72% • A tetrapeptide linked to a lipid for optimal skin penetration
The skin around the eye is firmer 76% • A low recommended dosage range : 0.3 to 2.5%
The expression is more rested 76% • Clinical efficacy
The skin around the eye is more moisturised 83% • Rapid visible improvement
The skin on the eye contour is smoother 83% B i o t e c n o l o g í a
Immediate well-being effect
SPECIFICATIONS
86%
The product is well tolerated
90% INCI NAME : Glycerin (1) (and) Water (2) (and) Dextran (3) (and) Caprooyl tetrapeptide-3 (4)
by the skin around the eye RECHERCHE
Positive Opinion (%) CAS NUMBER : (1) 56-81-5, (2) 7732-18-5, (3) 9004-54-0 BIOTECHNOLOGIE

Consumers agree that ChroNOlineTM makes the skin smoother,

EINECS NUMBER : (1) 200-289-5, (2) 231-791-2, (3) 232-677-5 DEVE
METALLOPROTÉINASS
PATENT : US60/947, 144
firmer and more even, for a younger rested look.
SCIENCE
ChroNOlineTM

FEATURES AND BENEFITS

ANTI-WRINKLE EYE SERUM FORMULATION B i o t e c h n o l o g y
FEATURES BENEFITS Ingredient %W/W Supplier COSMETIQUES
Demineralized water (Aqua) 59.65
Peptide Stable, easy to work with Cabopol Ultrez-10 0.06 Noveon
Butylene glycol 3.00
Small peptide linked to a lipid Optimal skin penetration
Glycerin 3.00
High efficacy Low dosage (0.3 to 2.5%) Phenonip (Phenoxyethanol, methylparaben, ethylparaben, butylparaben, propylparaben, 0.80 Nipa
isobutylparaben)
Ex vivo Efficacy on skin aging Rejuvenates aging skin
Dermol M-5 (Caprylic/Capric triglyceride) 10.00 Alzo
Wrinkle reduction, the skin looks firmer and Saboderm MM (Myristyl Myristate) 4.50 Sabo
Clinical Efficacy on individuals over 40 smoother after only 28 days Cerasynt SD (Glyceryl stearate) 3.00 ISP
Positive consumer perception Provides a younger and rested look with
immediate well-being effect
Tween 60 (Polysorbate 60)
Butyrospermum Parkii (shea butter) fruit
Arlacel (Sorbitan stearate)
3.00
1.50
0.75
Uniqema

Uniqema
CHRONOLINE TM

APPLICATIONS & RECOMMENDED DOSAGE Fluid 200, 300cs (Dimethicone) 0.60 Dow corning
Perfum Fleurarôme No 6270 0.03 Fleurarôme
ChroNOlineTM is easy to use and has an excellent safety profile. Triethanolamine 99% 0.11 Produits CCC
ChroNOlineTM is a preservative free peptide formulated in vegetable glycerine.* Demineralized water (Aqua) 7.50
ChroNOline™ 2.50 Atrium Innovations

Pour water into the main tank and add Ultrez. Then start stirring and heating. In a separate tank,
Draw the line
Intensive Preventive Skin repair
treatment: treatment: treatment:
prepare the premixture (Glycerine + Butylene glycol + Phenonip). Add this premixture to the
aqueous mixture. Heat up to 75°C. In a separate tank, heat oils under slow stirring and heat up to
on wrinkles
2.5% 1% to 2.5% 0.3% to 1% approx. 75-80°C. Add slowly (during 10 min) the oil phase into the vortex of the aqueous phase.
MIXING Stir vigorously for 15 min. at 75°C. Then begin cooling and decrease the stirring speed. Add the
- Daily anti-aging care
PROCEDURE fragrance and the TEA at approx. 40°C. Add the peptide solution into the emulsion and continue
stirring up to 30°C to obtain a smooth emulsion. Add water if necessary.
- Sun care
- Anti-wrinkle care
10-2007
- Men’s care - Dermocosmetic formulation Note: Mixer is used for stirring. Do not homogenize.
- Anti-aging care (in combination with corticoid treatment)
- In combination with age-spot
- Correcting serum - Make-up (foundation)
treatment
- Mature skin care - Wound healing
- 1st fine line & wrinkle care
- Specific care (eyes, lips, etc.)
- Firming and smoothing care
- Restoring and restructuring care www.atrium-innov.com cosmetic@atrium-innov.com
Incorporation mode: at the end of formulation at temperature of <40°C Canada 1405, boul. du Parc-Technologique, Québec (Québec) G1P 4P5 • Tel.: (418) 652-1116 • Fax: (418) 652-0151
Unipex La Défense 8, Paris, France 92042 • Tel.: +33.147.32.81.30 • Fax: +33.141.96.22.03 I N N O V A T I O N S
*WARNING: The content should be used immediately after first opening of container; aseptic conditions are recommended while transferring product.
Fiche ChroNOline 10/23/07 9:57 AM Page 4

After the end of World War II many countries around the world, notably in Europe, Asia-Pacific and North IN VITRO EFFICACY CLINICAL EFFICACY
America experienced a baby boom. Baby boomers are the largest age cohort in history. Baby boomers are
getting older and they are seeking out several ways to reduce and delay the numerous signs of aging or they In vitro experiments were done to demonstrate the boosting effect of ChroNOlineTM on DEJ essential structural glycoproteins.
simply want to age gracefully and look good for their age. Fibronectin anchors cells to the ECM and laminin anchors the epidermis to the dermis. Test Protocol:
• 27 female volunteers (age: 40 to 65)
Atrium is now offering an innovative solution to better answer their needs: ChroNOlineTM Laminin Expression Fibronectin Expression
2000
• Application of a placebo or ChroNOline™ 2.5%, 2 X/Day on crow’s feet (split face)
MECANISM OF ACTION 4 * • Wrinkle measurement with silicone rubber replica taken at D0, D28
+26%

Laminin (ng/μg proteins)

• Additional wrinkle measurement at D56 for sub-group 50-65 (14 volunteers)

Fibronectin (ng/ml)
Skin Aging +10% *
Genetic dispositions, UV exposure and pollution lead to the dynamic and complex phenomenon of skin 3
1500 +64%* +60%*
chronological aging. Skin aging is characterized by the loss of elasticity, support and firmness, skin Iconography Digital photography Histogram
fragility and appearance of fine lines and wrinkles.
1000
2
At the cellular level, skin aging leads to a decrease in epidermal thickness, combined with a flattening of
dermo-epidermal junction (DEJ) which decreases the amount of nutrients available to the epidermis thus * Statistically
Fast
interfering with the skin's natural repair process. 1
TM
500 significant when
compared to
control p<0.05
Action
Control TGF-β ChroNOline Control TGF-β ChroNOlineTM
Thinning of the skin 10-7M 10-7M
Wrinkle
ChroNOlineTM stimulates laminin and fibronectin, thus firmly anchors cells
D0
to the matrix for optimal cell function and maintenance of DEJ integrity.
ChroNOlineTM

ChroNOlineTM
Epidermis
EX VIVO EFFICACY 16%*
The DEJ provides structural and functional integrity to the skin. Unfortunately DEJ flattens and becomes linear during aging,
Reduction
contributing to wrinkle formation. Collagen VII and laminin-5 are major constituents of the DEJ. They both help to maintain of fine lines
skin cohesion thus preventing wrinkle formation. and wrinkles
(average reduction
DEJ flattening compared to the
With the end-purpose of evaluating the effect of ChroNOline , human skin explants exposed to corticoids were used as a
TM
placebo after 28 days)
* p<0.05
D28
Dermis DEJ

Collagen fragmentation
model to reproduce skin aging. Corticoids use may lead to serious side-effects such as skin atrophy.

Young Skin Aged Skin ChroNOlineTM provides a significant reduction of the appearance
Collagen VII Expression Laminin-5 Expression of fine lines and wrinkles after only 28 days.
Dermo-Epidermal Junction (DEJ)
2.5
2.5

The dermo-epidermal junction maintains skin cohesion +34%

+49%
and supports skin metabolic needs for a young and healthy 2
look. 2
Within the group, we observed the following results for those aged 50 to 65 (14 volunteers).

Score

Score
DEJ MAJOR COMPONENTS 1.5 -16%
1.5 Wrinkles reduction compared to placebo
Laminin-5: Anchors the epidermis to the dermis -45% 0
for structural support
Building
1
1
Collagen VII: Anchors the dermis to the DEJ Control Skin + Skin + corticoids Control Skin + Skin + corticoids -10
Effect

Reduction in %
for mechanical stability of the DEJ corticoids + ChroNOlineTM corticoids + ChroNOlineTM

Fibronectin: Anchors cells to ECM for cell adhesion

Control -20 -14%*
For older people, fine line
and wrinkle reduction
Skin + corticoids kept on progressing over
+ ChroNOlineTM
THE SOLUTION: ChroNOlineTM -30 2 months for an average
Skin + corticoids
Epidermis -27%* reduction of 27%.
ChroNOline is a biomemitic tetrapeptide derived from a growth factor. ChroNOline is linked to a lipid
TM TM DEJ * p<0.05
Fine lines Fine lines
for higher stability and optimal skin penetration. ChroNOline boosts the production of key components
TM
Demis & wrinkles & wrinkles
at the DEJ like collagen VII, laminin-5 and fibronectin for optimal skin structural support. ChroNOline TM
After 28 days After 56 days
provides outstanding reduction of the appearance of fine lines and wrinkles after only 28 days. No flattening as seen in control

With ChroNOlineTM, the skin looks smoother, ChroNOlineTM stimulates both collagen VII and laminin-5 to reinforce the normal ChroNOlineTM provides a continuous long term significant reduction
firmer and more even for a younger rested look. structure of the DEJ, preventing the flattening that occurs with aging. of the appearance of fine lines and wrinkles in mature skin.
Fiche ChroNOline 10/23/07 9:57 AM Page 4

After the end of World War II many countries around the world, notably in Europe, Asia-Pacific and North IN VITRO EFFICACY CLINICAL EFFICACY
America experienced a baby boom. Baby boomers are the largest age cohort in history. Baby boomers are
getting older and they are seeking out several ways to reduce and delay the numerous signs of aging or they In vitro experiments were done to demonstrate the boosting effect of ChroNOlineTM on DEJ essential structural glycoproteins.
simply want to age gracefully and look good for their age. Fibronectin anchors cells to the ECM and laminin anchors the epidermis to the dermis. Test Protocol:
• 27 female volunteers (age: 40 to 65)
Atrium is now offering an innovative solution to better answer their needs: ChroNOlineTM Laminin Expression Fibronectin Expression
2000
• Application of a placebo or ChroNOline™ 2.5%, 2 X/Day on crow’s feet (split face)
MECANISM OF ACTION 4 * • Wrinkle measurement with silicone rubber replica taken at D0, D28
+26%

Laminin (ng/μg proteins)

• Additional wrinkle measurement at D56 for sub-group 50-65 (14 volunteers)

Fibronectin (ng/ml)
Skin Aging +10% *
Genetic dispositions, UV exposure and pollution lead to the dynamic and complex phenomenon of skin 3
1500 +64%* +60%*
chronological aging. Skin aging is characterized by the loss of elasticity, support and firmness, skin Iconography Digital photography Histogram
fragility and appearance of fine lines and wrinkles.
1000
2
At the cellular level, skin aging leads to a decrease in epidermal thickness, combined with a flattening of
dermo-epidermal junction (DEJ) which decreases the amount of nutrients available to the epidermis thus * Statistically
Fast
interfering with the skin's natural repair process. 1
TM
500 significant when
compared to
control p<0.05
Action
Control TGF-β ChroNOline Control TGF-β ChroNOlineTM
Thinning of the skin 10-7M 10-7M
Wrinkle
ChroNOlineTM stimulates laminin and fibronectin, thus firmly anchors cells
D0
to the matrix for optimal cell function and maintenance of DEJ integrity.
ChroNOlineTM

ChroNOlineTM
Epidermis
EX VIVO EFFICACY 16%*
The DEJ provides structural and functional integrity to the skin. Unfortunately DEJ flattens and becomes linear during aging,
Reduction
contributing to wrinkle formation. Collagen VII and laminin-5 are major constituents of the DEJ. They both help to maintain of fine lines
skin cohesion thus preventing wrinkle formation. and wrinkles
(average reduction
DEJ flattening compared to the
With the end-purpose of evaluating the effect of ChroNOline , human skin explants exposed to corticoids were used as a
TM
placebo after 28 days)
* p<0.05
D28
Dermis DEJ

Collagen fragmentation
model to reproduce skin aging. Corticoids use may lead to serious side-effects such as skin atrophy.

Young Skin Aged Skin ChroNOlineTM provides a significant reduction of the appearance
Collagen VII Expression Laminin-5 Expression of fine lines and wrinkles after only 28 days.
Dermo-Epidermal Junction (DEJ)
2.5
2.5

The dermo-epidermal junction maintains skin cohesion +34%

+49%
and supports skin metabolic needs for a young and healthy 2
look. 2
Within the group, we observed the following results for those aged 50 to 65 (14 volunteers).

Score

Score
DEJ MAJOR COMPONENTS 1.5 -16%
1.5 Wrinkles reduction compared to placebo
Laminin-5: Anchors the epidermis to the dermis -45% 0
for structural support
Building
1
1
Collagen VII: Anchors the dermis to the DEJ Control Skin + Skin + corticoids Control Skin + Skin + corticoids -10
Effect

Reduction in %
for mechanical stability of the DEJ corticoids + ChroNOlineTM corticoids + ChroNOlineTM

Fibronectin: Anchors cells to ECM for cell adhesion

Control -20 -14%*
For older people, fine line
and wrinkle reduction
Skin + corticoids kept on progressing over
+ ChroNOlineTM
THE SOLUTION: ChroNOlineTM -30 2 months for an average
Skin + corticoids
Epidermis -27%* reduction of 27%.
ChroNOline is a biomemitic tetrapeptide derived from a growth factor. ChroNOline is linked to a lipid
TM TM DEJ * p<0.05
Fine lines Fine lines
for higher stability and optimal skin penetration. ChroNOline boosts the production of key components
TM
Demis & wrinkles & wrinkles
at the DEJ like collagen VII, laminin-5 and fibronectin for optimal skin structural support. ChroNOline TM
After 28 days After 56 days
provides outstanding reduction of the appearance of fine lines and wrinkles after only 28 days. No flattening as seen in control

With ChroNOlineTM, the skin looks smoother, ChroNOlineTM stimulates both collagen VII and laminin-5 to reinforce the normal ChroNOlineTM provides a continuous long term significant reduction
firmer and more even for a younger rested look. structure of the DEJ, preventing the flattening that occurs with aging. of the appearance of fine lines and wrinkles in mature skin.
Fiche ChroNOline 10/23/07 9:57 AM Page 4

After the end of World War II many countries around the world, notably in Europe, Asia-Pacific and North IN VITRO EFFICACY CLINICAL EFFICACY
America experienced a baby boom. Baby boomers are the largest age cohort in history. Baby boomers are
getting older and they are seeking out several ways to reduce and delay the numerous signs of aging or they In vitro experiments were done to demonstrate the boosting effect of ChroNOlineTM on DEJ essential structural glycoproteins.
simply want to age gracefully and look good for their age. Fibronectin anchors cells to the ECM and laminin anchors the epidermis to the dermis. Test Protocol:
• 27 female volunteers (age: 40 to 65)
Atrium is now offering an innovative solution to better answer their needs: ChroNOlineTM Laminin Expression Fibronectin Expression
2000
• Application of a placebo or ChroNOline™ 2.5%, 2 X/Day on crow’s feet (split face)
MECANISM OF ACTION 4 * • Wrinkle measurement with silicone rubber replica taken at D0, D28
+26%

Laminin (ng/μg proteins)

• Additional wrinkle measurement at D56 for sub-group 50-65 (14 volunteers)

Fibronectin (ng/ml)
Skin Aging +10% *
Genetic dispositions, UV exposure and pollution lead to the dynamic and complex phenomenon of skin 3
1500 +64%* +60%*
chronological aging. Skin aging is characterized by the loss of elasticity, support and firmness, skin Iconography Digital photography Histogram
fragility and appearance of fine lines and wrinkles.
1000
2
At the cellular level, skin aging leads to a decrease in epidermal thickness, combined with a flattening of
dermo-epidermal junction (DEJ) which decreases the amount of nutrients available to the epidermis thus * Statistically
Fast
interfering with the skin's natural repair process. 1
TM
500 significant when
compared to
control p<0.05
Action
Control TGF-β ChroNOline Control TGF-β ChroNOlineTM
Thinning of the skin 10-7M 10-7M
Wrinkle
ChroNOlineTM stimulates laminin and fibronectin, thus firmly anchors cells
D0
to the matrix for optimal cell function and maintenance of DEJ integrity.
ChroNOlineTM

ChroNOlineTM
Epidermis
EX VIVO EFFICACY 16%*
The DEJ provides structural and functional integrity to the skin. Unfortunately DEJ flattens and becomes linear during aging,
Reduction
contributing to wrinkle formation. Collagen VII and laminin-5 are major constituents of the DEJ. They both help to maintain of fine lines
skin cohesion thus preventing wrinkle formation. and wrinkles
(average reduction
DEJ flattening compared to the
With the end-purpose of evaluating the effect of ChroNOline , human skin explants exposed to corticoids were used as a
TM
placebo after 28 days)
* p<0.05
D28
Dermis DEJ

Collagen fragmentation
model to reproduce skin aging. Corticoids use may lead to serious side-effects such as skin atrophy.

Young Skin Aged Skin ChroNOlineTM provides a significant reduction of the appearance
Collagen VII Expression Laminin-5 Expression of fine lines and wrinkles after only 28 days.
Dermo-Epidermal Junction (DEJ)
2.5
2.5

The dermo-epidermal junction maintains skin cohesion +34%

+49%
and supports skin metabolic needs for a young and healthy 2
look. 2
Within the group, we observed the following results for those aged 50 to 65 (14 volunteers).

Score

Score
DEJ MAJOR COMPONENTS 1.5 -16%
1.5 Wrinkles reduction compared to placebo
Laminin-5: Anchors the epidermis to the dermis -45% 0
for structural support
Building
1
1
Collagen VII: Anchors the dermis to the DEJ Control Skin + Skin + corticoids Control Skin + Skin + corticoids -10
Effect

Reduction in %
for mechanical stability of the DEJ corticoids + ChroNOlineTM corticoids + ChroNOlineTM

Fibronectin: Anchors cells to ECM for cell adhesion

Control -20 -14%*
For older people, fine line
and wrinkle reduction
Skin + corticoids kept on progressing over
+ ChroNOlineTM
THE SOLUTION: ChroNOlineTM -30 2 months for an average
Skin + corticoids
Epidermis -27%* reduction of 27%.
ChroNOline is a biomemitic tetrapeptide derived from a growth factor. ChroNOline is linked to a lipid
TM TM DEJ * p<0.05
Fine lines Fine lines
for higher stability and optimal skin penetration. ChroNOline boosts the production of key components
TM
Demis & wrinkles & wrinkles
at the DEJ like collagen VII, laminin-5 and fibronectin for optimal skin structural support. ChroNOline TM
After 28 days After 56 days
provides outstanding reduction of the appearance of fine lines and wrinkles after only 28 days. No flattening as seen in control

With ChroNOlineTM, the skin looks smoother, ChroNOlineTM stimulates both collagen VII and laminin-5 to reinforce the normal ChroNOlineTM provides a continuous long term significant reduction
firmer and more even for a younger rested look. structure of the DEJ, preventing the flattening that occurs with aging. of the appearance of fine lines and wrinkles in mature skin.
Fiche ChroNOline 10/23/07 9:57 AM Page 1

CONSUMER TEST ChroNOlineTM

B i o t e c h n o l o g i e
Test performed on 30 consumers for a period of 56 days. The consumers were asked to apply the cream SUMMARY
(2.5% ChroNOlineTM) in the morning and at night.
• A new and innovative anti-aging peptide
The expression is younger 72% • A premium and patented product
The skin around the eye is more even 72% • A tetrapeptide linked to a lipid for optimal skin penetration
The skin around the eye is firmer 76% • A low recommended dosage range : 0.3 to 2.5%
The expression is more rested 76% • Clinical efficacy
The skin around the eye is more moisturised 83% • Rapid visible improvement
The skin on the eye contour is smoother 83% B i o t e c n o l o g í a
Immediate well-being effect
SPECIFICATIONS
86%
The product is well tolerated
90% INCI NAME : Glycerin (1) (and) Water (2) (and) Dextran (3) (and) Caprooyl tetrapeptide-3 (4)
by the skin around the eye RECHERCHE
Positive Opinion (%) CAS NUMBER : (1) 56-81-5, (2) 7732-18-5, (3) 9004-54-0 BIOTECHNOLOGIE

Consumers agree that ChroNOlineTM makes the skin smoother,

EINECS NUMBER : (1) 200-289-5, (2) 231-791-2, (3) 232-677-5 DEVE
METALLOPROTÉINASS
PATENT : US60/947, 144
firmer and more even, for a younger rested look.
SCIENCE
ChroNOlineTM

FEATURES AND BENEFITS

ANTI-WRINKLE EYE SERUM FORMULATION B i o t e c h n o l o g y
FEATURES BENEFITS Ingredient %W/W Supplier COSMETIQUES
Demineralized water (Aqua) 59.65
Peptide Stable, easy to work with Cabopol Ultrez-10 0.06 Noveon
Butylene glycol 3.00
Small peptide linked to a lipid Optimal skin penetration
Glycerin 3.00
High efficacy Low dosage (0.3 to 2.5%) Phenonip (Phenoxyethanol, methylparaben, ethylparaben, butylparaben, propylparaben, 0.80 Nipa
isobutylparaben)
Ex vivo Efficacy on skin aging Rejuvenates aging skin
Dermol M-5 (Caprylic/Capric triglyceride) 10.00 Alzo
Wrinkle reduction, the skin looks firmer and Saboderm MM (Myristyl Myristate) 4.50 Sabo
Clinical Efficacy on individuals over 40 smoother after only 28 days Cerasynt SD (Glyceryl stearate) 3.00 ISP
Positive consumer perception Provides a younger and rested look with
immediate well-being effect
Tween 60 (Polysorbate 60)
Butyrospermum Parkii (shea butter) fruit
Arlacel (Sorbitan stearate)
3.00
1.50
0.75
Uniqema

Uniqema
CHRONOLINE TM

APPLICATIONS & RECOMMENDED DOSAGE Fluid 200, 300cs (Dimethicone) 0.60 Dow corning
Perfum Fleurarôme No 6270 0.03 Fleurarôme
ChroNOlineTM is easy to use and has an excellent safety profile. Triethanolamine 99% 0.11 Produits CCC
ChroNOlineTM is a preservative free peptide formulated in vegetable glycerine.* Demineralized water (Aqua) 7.50
ChroNOline™ 2.50 Atrium Innovations

Pour water into the main tank and add Ultrez. Then start stirring and heating. In a separate tank,
Draw the line
Intensive Preventive Skin repair
treatment: treatment: treatment:
prepare the premixture (Glycerine + Butylene glycol + Phenonip). Add this premixture to the
aqueous mixture. Heat up to 75°C. In a separate tank, heat oils under slow stirring and heat up to
on wrinkles
2.5% 1% to 2.5% 0.3% to 1% approx. 75-80°C. Add slowly (during 10 min) the oil phase into the vortex of the aqueous phase.
MIXING Stir vigorously for 15 min. at 75°C. Then begin cooling and decrease the stirring speed. Add the
- Daily anti-aging care
PROCEDURE fragrance and the TEA at approx. 40°C. Add the peptide solution into the emulsion and continue
stirring up to 30°C to obtain a smooth emulsion. Add water if necessary.
- Sun care
- Anti-wrinkle care
10-2007
- Men’s care - Dermocosmetic formulation Note: Mixer is used for stirring. Do not homogenize.
- Anti-aging care (in combination with corticoid treatment)
- In combination with age-spot
- Correcting serum - Make-up (foundation)
treatment
- Mature skin care - Wound healing
- 1st fine line & wrinkle care
- Specific care (eyes, lips, etc.)
- Firming and smoothing care
- Restoring and restructuring care www.atrium-innov.com cosmetic@atrium-innov.com
Incorporation mode: at the end of formulation at temperature of <40°C Canada 1405, boul. du Parc-Technologique, Québec (Québec) G1P 4P5 • Tel.: (418) 652-1116 • Fax: (418) 652-0151
Unipex La Défense 8, Paris, France 92042 • Tel.: +33.147.32.81.30 • Fax: +33.141.96.22.03 I N N O V A T I O N S
*WARNING: The content should be used immediately after first opening of container; aseptic conditions are recommended while transferring product.
Fiche ChroNOline 10/23/07 9:57 AM Page 1

CONSUMER TEST ChroNOlineTM

B i o t e c h n o l o g i e
Test performed on 30 consumers for a period of 56 days. The consumers were asked to apply the cream SUMMARY
(2.5% ChroNOlineTM) in the morning and at night.
• A new and innovative anti-aging peptide
The expression is younger 72% • A premium and patented product
The skin around the eye is more even 72% • A tetrapeptide linked to a lipid for optimal skin penetration
The skin around the eye is firmer 76% • A low recommended dosage range : 0.3 to 2.5%
The expression is more rested 76% • Clinical efficacy
The skin around the eye is more moisturised 83% • Rapid visible improvement
The skin on the eye contour is smoother 83% B i o t e c n o l o g í a
Immediate well-being effect
SPECIFICATIONS
86%
The product is well tolerated
90% INCI NAME : Glycerin (1) (and) Water (2) (and) Dextran (3) (and) Caprooyl tetrapeptide-3 (4)
by the skin around the eye RECHERCHE
Positive Opinion (%) CAS NUMBER : (1) 56-81-5, (2) 7732-18-5, (3) 9004-54-0 BIOTECHNOLOGIE

Consumers agree that ChroNOlineTM makes the skin smoother,

EINECS NUMBER : (1) 200-289-5, (2) 231-791-2, (3) 232-677-5 DEVE
METALLOPROTÉINASS
PATENT : US60/947, 144
firmer and more even, for a younger rested look.
SCIENCE
ChroNOlineTM

FEATURES AND BENEFITS

ANTI-WRINKLE EYE SERUM FORMULATION B i o t e c h n o l o g y
FEATURES BENEFITS Ingredient %W/W Supplier COSMETIQUES
Demineralized water (Aqua) 59.65
Peptide Stable, easy to work with Cabopol Ultrez-10 0.06 Noveon
Butylene glycol 3.00
Small peptide linked to a lipid Optimal skin penetration
Glycerin 3.00
High efficacy Low dosage (0.3 to 2.5%) Phenonip (Phenoxyethanol, methylparaben, ethylparaben, butylparaben, propylparaben, 0.80 Nipa
isobutylparaben)
Ex vivo Efficacy on skin aging Rejuvenates aging skin
Dermol M-5 (Caprylic/Capric triglyceride) 10.00 Alzo
Wrinkle reduction, the skin looks firmer and Saboderm MM (Myristyl Myristate) 4.50 Sabo
Clinical Efficacy on individuals over 40 smoother after only 28 days Cerasynt SD (Glyceryl stearate) 3.00 ISP
Positive consumer perception Provides a younger and rested look with
immediate well-being effect
Tween 60 (Polysorbate 60)
Butyrospermum Parkii (shea butter) fruit
Arlacel (Sorbitan stearate)
3.00
1.50
0.75
Uniqema

Uniqema
CHRONOLINE TM

APPLICATIONS & RECOMMENDED DOSAGE Fluid 200, 300cs (Dimethicone) 0.60 Dow corning
Perfum Fleurarôme No 6270 0.03 Fleurarôme
ChroNOlineTM is easy to use and has an excellent safety profile. Triethanolamine 99% 0.11 Produits CCC
ChroNOlineTM is a preservative free peptide formulated in vegetable glycerine.* Demineralized water (Aqua) 7.50
ChroNOline™ 2.50 Atrium Innovations

Pour water into the main tank and add Ultrez. Then start stirring and heating. In a separate tank,
Draw the line
Intensive Preventive Skin repair
treatment: treatment: treatment:
prepare the premixture (Glycerine + Butylene glycol + Phenonip). Add this premixture to the
aqueous mixture. Heat up to 75°C. In a separate tank, heat oils under slow stirring and heat up to
on wrinkles
2.5% 1% to 2.5% 0.3% to 1% approx. 75-80°C. Add slowly (during 10 min) the oil phase into the vortex of the aqueous phase.
MIXING Stir vigorously for 15 min. at 75°C. Then begin cooling and decrease the stirring speed. Add the
- Daily anti-aging care
PROCEDURE fragrance and the TEA at approx. 40°C. Add the peptide solution into the emulsion and continue
stirring up to 30°C to obtain a smooth emulsion. Add water if necessary.
- Sun care
- Anti-wrinkle care
10-2007
- Men’s care - Dermocosmetic formulation Note: Mixer is used for stirring. Do not homogenize.
- Anti-aging care (in combination with corticoid treatment)
- In combination with age-spot
- Correcting serum - Make-up (foundation)
treatment
- Mature skin care - Wound healing
- 1st fine line & wrinkle care
- Specific care (eyes, lips, etc.)
- Firming and smoothing care
- Restoring and restructuring care www.atrium-innov.com cosmetic@atrium-innov.com
Incorporation mode: at the end of formulation at temperature of <40°C Canada 1405, boul. du Parc-Technologique, Québec (Québec) G1P 4P5 • Tel.: (418) 652-1116 • Fax: (418) 652-0151
Unipex La Défense 8, Paris, France 92042 • Tel.: +33.147.32.81.30 • Fax: +33.141.96.22.03 I N N O V A T I O N S
*WARNING: The content should be used immediately after first opening of container; aseptic conditions are recommended while transferring product.