Autoclaves: Options and Accessories
Autoclaves: Options and Accessories
Autoclaves: Options and Accessories
Autoclave (Greek/Latin: self-sealing): A gas-tight, sealable pressure vessel, used for the thermal
treatment of substances in the overpressure range. Autoclaves are used for sterilizing, curing building
materials, for vulcanising tires and belts and for pressing fiber composites. As a result, they are used in
medical and food technology, biology, the glass and aviation industries, as well as in brickworks and
vulcanising plants.
Autoclaves are based on the functional principle of “Papin’s pot”, developed by the French physicist
Denis Papin in 1674/79 and patented in 1681. Autoclaves are available for small volume applications but
there are also large-scale units for large production quantities. The substances are thermally treated in
batches (batch operation) because of the typical closure with respect to the surrounding atmosphere.
The pressure vessels are also normally equipped with quick-release closures that enable the pressure
vessel to be opened and closed much more quickly than with flanged pressure vessel openings.
As numerous as the fields of application for autoclaves are, so varied are the
available autoclaves and their options. Although autoclaves are built with the
same basic features, they do vary in shape, size and functionality, while
adhering to the same manufacturing rules and standards of performance and
core safety. Major laboratories and production facilities certainly place
different demands on autoclaves than highly specialised research facilities or
small labs with all-round applications do. Consequently, the decision to
purchase must be preceded by a detailed needs analysis that takes current and
future demands on the autoclave into account. Besides spatial and ergonomic
aspects, such as simple, careful operation and economic efficiency, as well as
safe, precise and above all reproducible and validatable sterilization, some
other important factors need to be considered.
Usage
Autoclaves provide a physical method for disinfection and sterilization. They work with a
combination of steam, pressure and time. Autoclaves operate at high temperature and
pressure in order to kill microorganisms and spores.
They are used to decontaminate certain biological waste and sterilize media,
instruments and lab ware. Regulated medical waste that might contain bacteria, viruses
and other biological material are recommended to be inactivated by autoclaving before
disposal.
To be effective, the autoclave must reach and maintain a temperature of 121° C
for at least 30 minutes by using saturated steam under at least 15 psi of pressure.
Increased cycle time may be necessary depending upon the make-up and volume of the
load.
The rate of exhaust will depend upon the nature of the load. Dry material can be
treated in a fast exhaust cycle, while liquids and biological waste require slow exhaust
to prevent boiling over of super-heated liquids.
https://microbeonline.com/autoclave-principle-procedure-types-and-uses/