Name: Date:

Student Exploration: Genetic Engineering

Vocabulary: callus, exon, genetic engineering, genetically modified organism, genome, green
fluorescent protein (GFP), herbicide, insecticide, intron, promoter, transcription, transformation

Prior Knowledge Questions (Do these BEFORE using the Gizmo.)

1. What are some things that can damage a farmer’s crops?

Various types of weeds and pests, insects, severe weather conditions (droughts, tornadoes, etc.)

air and water pollution.

2. What can farmers do to protect their crops?

Many farmers use chemicals like pesticides, herbicides, and fungicides. Farmers

can also use fertilizers to help increase the amount of nutrients in the soil.

Gizmo Warm-up
Many farmers use chemical herbicides to kill weeds and
insecticides to kill insects. Using genetic engineering,
scientists have developed ways to resist harmful crop pests.
In the Genetic Engineering Gizmo, you will use genetic
engineering techniques to create genetically modified corn.

Check that Task 1 is selected. The Gizmo shows petri dishes that contain different strains of
bacteria (white dots) and caterpillars (Lepidoptera sp. larvae). In the first challenge, your goal is
to find bacteria that produce toxins that kill the caterpillars. Click Play ( ).

1. What do you observe? 11 caterpillars died while 37 caterpillars lived.

2. Which strains of bacteria were able to kill Lepidoptera sp. larvae? 4,5,8,10

Were some more effective than others? Explain.

Some were: 4 and 8 killed all four; 5 killed two; 10 killed one

Some bacteria are able to produce a toxin that kills Lepidoptera sp. larvae. Find out which
gene is responsible for this toxin in the next step.

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 Activity A: Get the Gizmo ready:
Caterpillar-  Click Reset ( ) and check that Task 1 is selected
resistant corn in the dropdown menu.

Introduction: Lepidoptera sp. larvae (caterpillars) eat corn kernels, leaves, and stalks. In this
activity, use genetic engineering techniques to create a corn plant that is resistant to caterpillars.

Question: How can we produce corn that is resistant to Lepidoptera sp. larvae?

1. Observe: Click Play. Select one of the strains of bacteria harmful to larvae (by clicking on
the plate).

Which strain did you select? 8

2. Investigate: Click Continue. The screen now shows the genome, or set of genes, of the
selected bacteria. One of these genes produces the protein that kills the caterpillars. You
will test each gene by adding it to the genome of a bacteria that does not kill caterpillars.
This process is called transformation.

Drag three genes into the Petri dishes at lower right. These genes are now inserted into the
genomes of the sensitive bacteria in the plates. Press Play. If none of those genes help to
kill the caterpillars, click Reset and try three other genes. When you find a gene that kills the
caterpillars, click on the Petri dish to select the gene that confers resistance.

Which gene did you select? Gene F

In reality, finding a gene with a desired trait is much less common. Scientists search through
many more bacterial strains and potential genes to find the traits they are looking for.

3. Observe: Click Continue. Promoters are regions of DNA that initiate the transcription of a
gene. Some promoters only work in specific types of cells, such as leaf cells or root cells.

To determine which cells of a corn plant a promoter works in, four promoters have been
attached to the Green Fluorescent Protein (GFP) gene. Each promoter-GFP gene has
been inserted into a corn plant. Select Lights off to see the parts of each plant glow green
and fill in the table below.

Promoter Glowing plant part(s) Gene Glowing plant part(s)

Which promoter is active in only the leaves? 4 In the whole plant? 1

Select the promoter you would like to use by clicking on a plant, and then click Continue.

(Activity A continued on next page)

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Activity A (continued from previous page)

4. Choose: The resistance gene that was chosen in step 2 was attached to the promoter
chosen in step 3, and the new DNA was inserted into five calluses. A callus is a group of
cells that will incorporate the new gene into their genome and grow into a mature corn plant.

In each genome, genes are shown as green bars. Each gene contains light green exons, or
sections that code for proteins, and medium green introns, which do not code for proteins.
The dark green bars represent promoters and the red bars represent gene termination sites.

Use the left and right arrow buttons to observe where the new gene (blue bar) was inserted
into each of the corn calluses genomes. Problems can occur if the new gene is inserted into
the middle of an existing corn gene (green bar).

In which calluses did the new gene insert inside an existing corn gene? Callus 4

Select one of the corn calluses that do not disrupt an existing corn gene and click Continue.

5. Experiment: On the left is a control plant that does not contain any new genes. On the right
is the transformed plant you created. Click Play. When the plant has finished growing, click
on each of the circles to observe the leaves, cobs, and roots of each plant.

A. Did the transformed plant grow into a healthy mature plant? Yes

If not, you may have chosen a bad callus. (Click Back to try a different callus.)

B. Click Reset and select Add Lepidoptera sp. larvae for each plant. Click Play. What

do you observe? Control plant was eaten by the Lepidoptera sp. larvae while

the transformed plant was not affected by the larvae.

C. Compare the up-close views. How do the roots, leaves, and cobs compare?

The roots are the same. The leaves and cobs of the control plant are being

destroyed by the larvae while the leaves and cobs of the transformed plant

are not affected.

D. Select Show statistics. How did the results for the transformed plant differ from the

control plant? The control plant had a higher pest population and a smaller

corn yield than the transformed plant.

E. Click Submit for review. Was your plant resistant to Lepidoptera? Yes

If not, click Back or Start again. Be sure to choose genes that kill bacteria and a
promoter that protects the corn cobs, leaves, and stalks.

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 Activity B: Get the Gizmo ready:
Beetle grub-  Click Start again to reset the Gizmo.
resistant corn  Select Task 2 in the dropdown menu.

Introduction: Coleoptera sp. larvae are immature beetles. They feed on corn plant roots. Your
goal in this challenge is to create corn that is resistant to Coleoptera sp. larvae.

Question: How can we produce corn that is resistant to Coleoptera sp. larvae?

1. Investigate: Using the Gizmo, select a bacterial strain that kills Coleoptera and determine
the gene that will be used to develop resistance in the corn. Which choices did you make?

Bacterial strain: 6 Gene: F

Click Continue to move on to the “Choose promoter” step.

2. Hypothesize: Turn the room lights off. Beetle larvae attack the roots of corn plants. Based

on this, which promoters do you think would be effective against beetles? Promoter 1

Explain your reasoning. The roots are glowing.

3. Apply: Knowing that the new corn strain will be eaten by humans, which promoter might be

safer to use, and why? Promoter 3 might be safer to use because only the roots are

affected by the GFP gene

Select this promoter and click Continue.

4. Observe: Select a corn callus that you think will work and click Continue. On the next
screen, add Coleoptera sp. larvae to each plant and click Play.

A. Describe the control plant and the transformed plant. Control plant- didn't fully

grow. Produced corn but the roots were infested with larvae.

Transformed plant- not affected at all by the larvae and grew to full height

B. Select Show statistics and Submit for review. Is the experimental plant resistant to

Coleoptera sp. larvae? Yes

(Activity B continued on next page)

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Activity B (continued from previous page)

5. Explore: Click Back and select a corn callus in which the new gene (blue bar) is inserted in
the middle of an existing gene (green bar).

A. Click Continue. Grow the experimental plant with and without larvae. What do you

observe? With larvae- roots not affected, less corn yeild

Without larvae- multiple corn in one stalk

B. Click Back and choose another callus in which an existing gene is disrupted. What

do you observe? Corn grew dark (not natural color)

Note that these are dramatic examples of mutations. Complex organisms often have
many genes that can perform similar functions, so disrupting one gene may not
cause a noticeable change to the phenotype of the plant.

6. Explore: Click the Back button twice until the Choose promoter step is shown. Use the
Gizmo to test the effectiveness of each promoter.

Which promoters were effective in creating beetle-resistant corn, and why? 3, 4, and 1

because their corn not affected by the GFP.

7. Explore: Click Start again. This time, choose a bacterial strain in step 1 that only kills some
of the larvae. Grow the experimental plant in the presence and absence of larvae.

How does this plant compare to the plant you created in part 4 of this activity?

The roots are affected by the larvae and the plant does not grow to be healthy.

8. Think and discuss: What are some of the possible benefits of creating insect-resistant corn,
and what are some of the possible drawbacks? If possible, discuss your answer with your
classmates and teacher.

The corn yield would be higher but a drawback would be it could cause allergic reactions

or other health scares. It also could be a contributing factor to antibiotic resistance or

it could be a carcinogen.

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 Activity C: Get the Gizmo ready:
Herbicide-  Click Start again to reset the Gizmo.
resistant corn  Select Task 3 in the dropdown menu.

Introduction: Weeds are wild plants that compete with crops for resources. Farmers kill weeds
using herbicides, but corn plants may also be damaged by herbicides. Herbicides affect the
roots, stalks, leaves, and cobs of corn plants.

Question: How can we produce a corn plant resistant to herbicide?

1. Observe: Bacterial colonies are being grown in Petri dishes. The white disks on each dish
have been soaked in an herbicide.

Click Play. Describe what happens to the bacteria in the Petri dishes. Some of the bacteria

has survived while others have died.

Which strains of bacteria are not affected by the herbicide? 3, 7, 9

2. Observe: Choose a bacterial strain that is resistant to herbicide, find the gene that is
responsible for the resistance, choose a promoter, and transform a corn plant. Observe the
control and experimental plants in the presence and absence of herbicide.

When you have created an herbicide-resistant plant, fill in your choices below. (Note: you
may need to try a few promoters before finding the correct one.)

A. Which bacterial strain did you choose? 3

Which gene did you choose? Gene G

Which promoter did you choose? One

Which callus did you choose? 4

B. Describe the control and experimental plants.

Control- healthy and grown to full height

Transformed plant- healthy and grown to full height

C. Select Show statistics. How do the results from the transformed plant differ from the

control plant? Explain.

Transformed Plant has less of a corn yield and is also smaller.

(Activity C continued on next page)

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Activity C (continued from previous page)

3. Experiment: Go back two steps and experiment with different promoters. Can any of the

other promoters be used to create a resistant corn plant? All of them

Why or why not? All of the corn plants are resistant because the gene for the plant

allows it to be resistant with all the different promoters.

4. Analyze: What are some of the benefits of growing herbicide-resistant corn?

Weeds will not be harmful to the herbicide corn, and neither will the herbicides used

to kill weeds.

5. Analyze: Are there any possible drawbacks to having an herbicide-resistant corn plant?

Allergic reactions or carcinogens in the herbicide-resistant corn plant.

6. Think and discuss: Herbicides and insecticides can be bad for the environment. Insecticides
could harm beneficial insects like bees, and both herbicides and insecticides can
contaminate nearby rivers and streams.

A. What are some of the possible environmental benefits of GM crops?

Reduces the amount of pesticides that need to be sprayed, increases yield

of crops.

B. What are some of the possible environmental problems that can be caused by GM

crops? Hybrid of GM crops and non-GM crops.

C. What are some of the potential risks to humans and animals that eat GM crops?

Allergic reactions or carcinogens that are harmful to humans or animals.

The properties of the plant could be toxic once modified.

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