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    Name: Marniel S Patcho Date: Course and Section: III AGRONOMY Exercise 4 Microorganisms in The Environment

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    Marniel Patcho
    The document describes an exercise to observe microorganisms found in the environment. Students are instructed to expose nutrient agar (NA) and potato dextrose agar (PDA) plates in assigned areas for 10 minutes, then incubate the NA plates upside down and PDA plates upright at room temperature. After incubation, photos will be taken of any microbial colonies that have grown on the plates. Common microorganisms that can grow on NA include bacteria like streptococcus and staphylococcus, while PDA is used to cultivate fungi given its nutritionally rich potato base. Incubating NA upside down and PDA upright helps prevent contamination and condensation that could disturb the cultures.

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    Name: Marniel S Patcho Date: Course and Section: III AGRONOMY Exercise 4 Microorganisms in The Environment

    Uploaded by

    Marniel Patcho
    14 views2 pages
    The document describes an exercise to observe microorganisms found in the environment. Students are instructed to expose nutrient agar (NA) and potato dextrose agar (PDA) plates in assigned areas for 10 minutes, then incubate the NA plates upside down and PDA plates upright at room temperature. After incubation, photos will be taken of any microbial colonies that have grown on the plates. Common microorganisms that can grow on NA include bacteria like streptococcus and staphylococcus, while PDA is used to cultivate fungi given its nutritionally rich potato base. Incubating NA upside down and PDA upright helps prevent contamination and condensation that could disturb the cultures.

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    Microorganism

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    The document describes an exercise to observe microorganisms found in the environment. Students are instructed to expose nutrient agar (NA) and potato dextrose agar (PDA) plates in assigned areas for 10 minutes, then incubate the NA plates upside down and PDA plates upright at room temperature. After incubation, photos will be taken of any microbial colonies that have grown on the plates. Common microorganisms that can grow on NA include bacteria like streptococcus and staphylococcus, while PDA is used to cultivate fungi given its nutritionally rich potato base. Incubating NA upside down and PDA upright helps prevent contamination and condensation that could disturb the cultures.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    14 views2 pages

    Name: Marniel S Patcho Date: Course and Section: III AGRONOMY Exercise 4 Microorganisms in The Environment

    Uploaded by

    Marniel Patcho
    The document describes an exercise to observe microorganisms found in the environment. Students are instructed to expose nutrient agar (NA) and potato dextrose agar (PDA) plates in assigned areas for 10 minutes, then incubate the NA plates upside down and PDA plates upright at room temperature. After incubation, photos will be taken of any microbial colonies that have grown on the plates. Common microorganisms that can grow on NA include bacteria like streptococcus and staphylococcus, while PDA is used to cultivate fungi given its nutritionally rich potato base. Incubating NA upside down and PDA upright helps prevent contamination and condensation that could disturb the cultures.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
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    Name: MARNIEL S PATCHO Date:

    Course and Section: III AGRONOMY

    Exercise 4
    Microorganisms in the Environment

    Introduction

    Microorganisms are found everywhere – in the environment and in our bodies. Even the most
    unlikely places on earth – like thermal vents, the saltiest seas and the coldest regions – harbor
    microorganisms too small to be seen by the naked eye. Only the inner, healthy tissues of plants and
    animals are free of microorganisms. In this exercise, you will examine samples of microorganisms
    found in your surroundings and will attempt to grow them in laboratory media.

    Objectives

    After completing this exercise, you should be able to:


    1. observe the ubiquity of microorganisms; and
    2. learn how microorganisms are grown in the laboratory.

    Procedure
    1. For every group, obtain 1 Nutrient Agar (NA)plate and 1 Potato Dextrose Agar (PDA)plate.
    2. Expose NA and PDA plates in your assigned areas for 10 min.
    3. Incubate the exposed NAupside down at room temperature for 24 – 48 h and the exposed
    PDA (upright position)at room temperature for 5 days. After the incubation period,
    refrigerate the plates to prevent furthergrowthof the microorganisms.
    4. Without opening the agar plates, take a photo of the obverse (top) and reverse (bottom)
    side of the microbial colonies.

    Guide Questions

    1. What microorganisms may be found in the air or in the environment around us?

    We have the viruses must infect cells and use components of the host cell to make
    copies of themselves. Often, they kill the host cell in the process, and cause damage
    to the host organism.
    We have also the fungi is any member of the group of eukaryotic organisms that
    includes microorganisms such as yeasts and molds, as well as the more familiar
    mushrooms. These organisms are classified as a kingdom, which is separate from the
    other eukaryotic life kingdoms of plants and animals.

    2. What type of microorganisms will grow in NA?

    Microorganisms need food, water and a suitable environment in order to survive and
    grow. Nutrient agar provides these resources for many types of microbes, from fungi
    like yeast and mold to common bacteria such as streptococcus and staphylococcus.
    The microbes that can be grown on complex media such as nutrient agar can be
    described as no fastidious organism. It is organism microbes that are able to grow
    and thrive without special nutritional or environment conditions.
    3. What type of microorganisms will grow in PDA?

    Potato Dextrose Agar (PDA) is used for the cultivation of fungi. Potato Dextrose Agar
    (PDA) is a general purpose medium for yeasts and molds that can be
    supplemented with acid or antibiotics to inhibit bacterial growth. It is recommended
    for plate count methods for foods, dairy products and testing cosmetics. PDA can be
    used for growing clinically significant yeast and molds. The nutritionally rich base
    (potato infusion) encourages mold sporulation and pigment production in
    some dermatophytes.

    4. What is the purpose of incubating the NA plates upside down?

    Petri dishes need to be incubated upside-down to lessen contamination risks from


    airborne particles landing on them and to prevent the accumulation of water
    condensation that could disturb or compromise a culture.

    5. What is the purpose of incubating the PDA plates in upright position?

    During storage (in refrigerator, but not to freeze) the agar plates should be


    placed in an inverted position with the lid at the bottom. This prevents the
    condensation from dripping down on to the surface of the agar, which may
    allow for the movement of the organisms from one colony to another.

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