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    Mcfarland Standards: PRODUCT CODE SD2350, SD2300, SD2301, SD2302, SD2303, SD2304

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    SOKAMTE TEGANG Alphonse
    The McFarland Standard tubes contain latex particles suspended in buffer that are adjusted to standard turbidity levels using a spectrophotometer. These standards are used to adjust the density of bacterial suspensions to produce expected plate counts for identification and susceptibility testing. A bacterial suspension that matches the turbidity of a McFarland Standard tube will have a specific approximate cell count density. The standards provide consistent and reproducible suspensions to control the quality of diagnostic microbiology tests.

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    Mcfarland Standards: PRODUCT CODE SD2350, SD2300, SD2301, SD2302, SD2303, SD2304

    Uploaded by

    SOKAMTE TEGANG Alphonse
    201 views1 page
    The McFarland Standard tubes contain latex particles suspended in buffer that are adjusted to standard turbidity levels using a spectrophotometer. These standards are used to adjust the density of bacterial suspensions to produce expected plate counts for identification and susceptibility testing. A bacterial suspension that matches the turbidity of a McFarland Standard tube will have a specific approximate cell count density. The standards provide consistent and reproducible suspensions to control the quality of diagnostic microbiology tests.

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    Préparation de l'étalon de McFarland

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    McFarland

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    The McFarland Standard tubes contain latex particles suspended in buffer that are adjusted to standard turbidity levels using a spectrophotometer. These standards are used to adjust the density of bacterial suspensions to produce expected plate counts for identification and susceptibility testing. A bacterial suspension that matches the turbidity of a McFarland Standard tube will have a specific approximate cell count density. The standards provide consistent and reproducible suspensions to control the quality of diagnostic microbiology tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    201 views1 page

    Mcfarland Standards: PRODUCT CODE SD2350, SD2300, SD2301, SD2302, SD2303, SD2304

    Uploaded by

    SOKAMTE TEGANG Alphonse
    The McFarland Standard tubes contain latex particles suspended in buffer that are adjusted to standard turbidity levels using a spectrophotometer. These standards are used to adjust the density of bacterial suspensions to produce expected plate counts for identification and susceptibility testing. A bacterial suspension that matches the turbidity of a McFarland Standard tube will have a specific approximate cell count density. The standards provide consistent and reproducible suspensions to control the quality of diagnostic microbiology tests.

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    MCFARLAND STANDARDS PRODUCT CODE SD2350, SD2300, SD2301,

    SD2302, SD2303, SD2304

    INTENDED USE PRECAUTIONS SAFETY


    The McFarland Equivalence Standards are intended to be part 1. Do not use product beyond the expiration date. See MSDS for additional information.
    of a quality control program for adjusting densities of bacterial 2. Directions should be carefully read prior to use.
    suspensions that are used for identification and susceptibility 3. The same size tube should be used in comparing bacterial
    testing. Each standard is made from different concentrations suspensions to the McFarland Standards. REFERENCES
    of latex beads mixed in a buffer liquid. The original McFarland 1. McFarland, J., J.Amer.Med.Assoc. 14:1176, 1907.
    Standards were made from the combination of Barium chlo- 2. NCCLS Document, Performance Standards for Antimicrobial
    ride and Sulfuric acid that result in a flocculate. Problems were TEST PROTOCOL Disk Susceptibility Tests. 4th ed. 10:7, p 10, 1990.
    encountered with this technique which included instability, 1. Prior to use, gently invert the McFarland Equivalence
    storage, and reproducibility of the resulting suspension. These Standard tube several times to assure uniformity of the sus-
    problems have been overcome by using latex particles in a buf- pension of latex particles.
    fer solution to make Colorimeter and McFarland Standards. 2. Adjust the turbidity of the log growth of the bacterial sus-
    pension to that of a known McFarland Equivalence Standard.
    3. Compare the turbidity by holding bacterial sample and
    SUMMARY AND EXPLANATION McFarland Standard tubes up against the black and white
    The McFarland Standard tubes contain latex particles suspend- bars printed on enclosed card.
    ed in a special buffer that are adjusted to an acceptable trans-
    mission range using a spectrophotometer at a wave length of
    either 600 or 625 nm. A bacterial suspension once adjusted to QUALITY CONTROL
    the same turbidity of a McFarland Standard produces expected Each lot of McFarland Equivalence Standard Set is tested and
    bacterial plate counts and can be used in a variety of identifica- results fall into a tight range of acceptability.
    tion or susceptibility kits and methods.

    INTERPRETATION OF RESULTS
    PRINCIPLE OF THE PROCEDURE Equal disappearance or distortion of the black bar indicates a
    The McFarland Equivalence Standards are used for adjusting similar turbidity.
    densities of bacterial suspensions.
    McFarland Standard Approximate Cell Count
    Density (x108 cells)
    MATERIALS PROVIDED
    0.5 1.5
    Catalogue Number Description
    1.0 3.0
    SD2350 McFarland Standard Set - Containing
    one of each 0.5, 1.0, 2.0, 3.0, 4.0 2.0 6.0
    SD2300 McFarland Standard 0.5 3.0 9.0
    SD2301 McFarland Standard 1.0 4.0 12.0
    SD2302 McFarland Standard 2.0
    SD2303 McFarland Standard 3.0 LIMITATION OF THE PROCEDURE
    SD2304 McFarland Standard 4.0 1. Coloured media may not provide the proper contrast with
    McFarland Equivalence Standards. Incorrect results will occur.
    2. Bacterial suspensions of older cultures may not compare to
    STABILITY AND STORAGE expected bacterial counts.
    All components should be stored 15°-30°C. Do not freeze. 3. These standards have been adjusted by a spectrophotom-
    Components stored under these conditions will be stable until eter. Use of any other instrumentation may not give reliable
    the expiration date shown on the label. results.

    Revision: 2012 05

    Canada 800 268 2341 Fax 905 731 0206 U.S.A. 800 522 7740 Fax 800 332 0450 U.K. 0151 353 1613 Fax 0151 353 1614
    20 Mural Street, Unit #4, Richmond Hill, ON, L4B 1K3 21 Cypress Blvd., Suite 1070, Round Rock, TX, 78665-1034 3 Bassendale Road, Bromborough, Wirral, Merseyside, CH62 3QL

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