Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Scribd Logo
Upload

Welcome to Scribd!

  • 965 views

    TOTAL PROTEIN Liquicolor

    Uploaded by

    Maher
    This document provides instructions for using the Total Protein Iiquicolor test kit to determine the total protein concentration in serum or plasma samples. The test uses a biuret method where copper ions react with proteins in an alkaline solution to form a purple complex, and the absorbance is proportional to the protein concentration. The test is linear up to 12 g/dL protein and quality controls are recommended to validate results. Normal ranges for total protein in serum/plasma are provided.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    TOTAL PROTEIN Liquicolor

    Uploaded by

    Maher
    965 views1 page
    This document provides instructions for using the Total Protein Iiquicolor test kit to determine the total protein concentration in serum or plasma samples. The test uses a biuret method where copper ions react with proteins in an alkaline solution to form a purple complex, and the absorbance is proportional to the protein concentration. The test is linear up to 12 g/dL protein and quality controls are recommended to validate results. Normal ranges for total protein in serum/plasma are provided.

    Original Title

    TOTAL PROTEIN liquicolor

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides instructions for using the Total Protein Iiquicolor test kit to determine the total protein concentration in serum or plasma samples. The test uses a biuret method where copper ions react with proteins in an alkaline solution to form a purple complex, and the absorbance is proportional to the protein concentration. The test is linear up to 12 g/dL protein and quality controls are recommended to validate results. Normal ranges for total protein in serum/plasma are provided.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    965 views1 page

    TOTAL PROTEIN Liquicolor

    Uploaded by

    Maher
    This document provides instructions for using the Total Protein Iiquicolor test kit to determine the total protein concentration in serum or plasma samples. The test uses a biuret method where copper ions react with proteins in an alkaline solution to form a purple complex, and the absorbance is proportional to the protein concentration. The test is linear up to 12 g/dL protein and quality controls are recommended to validate results. Normal ranges for total protein in serum/plasma are provided.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Total Protein Iiquicolor Performance Characteristics

    Linearity:
    Photometric Colorimetric Test for Total The test is linear up to a protein concentration of 12 g/dl or 120 g/l. Dilute
    Proteins samples with a higher concentration 1 + 1 with physiological saline (0.9%).
    Multiply the result by 2.
    Biuret Method Typical performance data can be found in the Verification Report, acces-
    Package Size sible via:
    [REF] 10570 1 x 1000 ml Complete Test Kit www.human.de/data/gb/vr/su-prot.pdf or
    157004 4 x 100 ml Complete Test Kit www.human-de.com/data/gb/vr/su-prot.pdf
    [IVD]
    Normal Range in Serum or Plasma 2
    Method Normal born babies 4.6 - 7.0 g/dl or 46 - 70 g/l
    Cupric ions react with protein in alkaline solution to form a purple com- Children from 3 years and adults 6.6 - 8.7 g/dl or 66 - 87 g/l
    plex. The absorbance of this complex is proportional to the protein con-
    centration in the sample. Quality Control
    All control sera with values determined by this method may be employed.
    Contents
    We recommend the use of our HUMATROL quality control serum based on
    [RGT] 4 x 100 ml or 1 x 1000 ml Colour reagent
    animal serum or our SERODOS based on human serum.
    Sodium hydroxide 200 mmoI/l
    Potassium sodium tartrate 32 mmol/l Automation
    Copper sulfate 12 mmol/l Proposals to apply the reagents on analyzers are available on request.
    Potassium iodide 30 mmoI/l Each laboratory has to validate the application in its own responsibility.
    Irritant R 36/38
    Notes
    [STD] 1 x 3 ml Standard
    1. The sample blank for clear, colourless sera is equivalent to 0.2 g/dl and
    Protein 8 g/dI can therefore be ignored. A sample blank must be determined for visi-
    or 80 g/l bly hemolytic, icteric and lipemic sera by pipetting 20 μl sample to 1000
    Sodium azide 0.095 % μl physiological saline and measuring against dist. water. The
    absorbance of the sample blank has to be subtracted from the absorb-
    Reagent Preparation and Stability
    ance of the sample.
    [RGT] and [STD] are ready for use.
    2. The colour reagent contains sodium hydroxide which is irritant. In case
    They are stable even after opening up to the given expiry date when of contact with skin and mucous membranes wash with copious
    stored at 2...25°C. Contamination after opening must be avoided. amounts of water.
    Specimen 3. [STD] contains sodium azide as preservative (0.095%). Do not swallow.
    Serum, heparinised or EDTA-plasma. Avoid contact with skin and mucous membranes.
    4. A slight sediment may develop as [RGT] ages. Do not include this
    Stability in Serum sediment in the reaction mixture.
    Up to 1 month at 2...8°C, up to 1 week at 15...25°C.
    References
    Assay 1. Weichselbaum, T. E., Amer. J. Clin. Path. 16, 40-48 (1946)
    Wavelength: Hg 546 nm, 520 – 580 nm
    2. Josephson, B., Gyllenswärd, C., Scand. J. Clin. Lab. Invest. 9, 29 (1957)
    Optical path: 1 cm
    Temperature: 20...25°C SU-PROT INF 157001 GB 05-2007-16 |
    Measurement: Against reagent blank.
    OnIy one reagent blank per series is required.

    Pipetting Scheme
    Pipette into cuvettes Reagent blank Sample / [STD]
    Sample / [STD] --- 20 μl
    [RGT] 1000 μl 1000 μl
    Mix, incubate for 10 min. at 20...25°C. Measure the absorbance of the
    sample and [STD] against the reagent blank within 30 min. (ΔA)

    Calculation of the Protein Concentration


    1. With factor:
    C = 19 x ΔA [g/dl] or C = 190 x ΔA [g/l]

    2. With standard:
    ΔA sample
    C = 8 x ⎯⎯⎯⎯⎯⎯⎯ [g/dl]
    ΔA [STD]
    or
    ΔA sample
    C = 80 x ⎯⎯⎯⎯⎯⎯⎯ [g/l]
    ΔA [STD]

    Human Gesellschaft für Biochemica und Diagnostica mbH


    Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
    Telefon +49 6122-9988-0 · Telefax +49 6122-9988-100 · e-Mail human@human.de

    You might also like