Curriculum Vitae

Name
Titik Nuryastuti, M.D., M.Sc., Ph.D., Sp.MK(K) [Consultant of Clinical Micobiologist]

Date and Place of Birth:

Affiliation
Department of Microbiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah
Mada, Yogyakarta, Indonesia.
Jl Farmako Yogyakarta Indonesia 55281
Phone: +62-274-580297
Fax: +62-274-413268
Email : t.nuryastuti@ugm.ac.id

Education
Faculty of Medicine, UGM Medical Doctor M.D., 1997
Biotechnology Research, UGM Magister of Biotechnology. M.Sc., 2001
University Medical Center of Groningen Biomedical Engineering Ph.D., 2010
Groningen, The Netherlands.
Faculty of Medicine, UGM Clinical Microbiology Specialist Sp.MK, 2014
Association of Indonesian Clinical Microbiologist SpMK(K), 2018
(Bacteriology Consultant)

Appointments
1997-now Lecturer at Department of Microbiology, Faculty of Medicine-PHN, UGM.
Teaching for undergraduate, Post doctoral (Master and PhD Program)
2012-now PIC of Tuberculosis Laboratory (BSL 2-enhanced Lab) in Fac of Medicine-PHN
UGM.
2016-2021 Secretary of Microbiology Department, Faculty of Medicine-PHN, UGM.
2020-now Head of Covid Laboratory in Faculty of Medicine-PHN, UGM

Field of interest:
Antimicrobial Resistance; Biofilm Related Infection; Antimicrobe Antibiofilm Exploration;
Mycobacterium Research; Hospital Acquired Infections, Pathogenesis of SARS-CoV-2
Publications
1. Titik Nuryastuti, Henny C. van der Mei, H.J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, 2008. recA mediated spontaneous deletions of the icaADBC operon of clinical S.
epidermidis isolates: a new mechanism of phenotypic variations. Antonie van
Leuwanhoek Journal 94:317-328, DOI 10.1007/s10482-008-9249-8.
2. Titik Nuryastuti, Henny C. van der Mei, H.J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, 2008. The role of genotypic changes on the development of phenotypic variations
in clinical isolates of Staphylococcus epidermidis. The Medical Journal of Malaysia Vol 63
Suppl A
3. Titik Nuryastuti, Henny C. van der Mei, H.J. Busscher, Iravati S, A.T.Aman, Bastiaan P.
Krom, 2009. Effect of cinnamon oil on icaA expression and biofilm formation by
Staphylococcus epidermidis. Applied and Environmental Microbiology, 75(21): 6850-
6855.
4. Titik Nuryastuti, 2010. Environmental signals affecting ica-expression in Staphylococcus
epidermidis biofilms, Ph.D. Thesis, University Medical Center Groningen, Groningen, The
Netherlands, ISBN No: 978-90-367-4420-1.
5. Titik Nuryastuti, Bastiaan P. Krom, H.J. Busscher, A.T.Aman, Henny C. van der Mei
2011. ica-expression and gentamicin susceptibility of S. epidermidis biofilm on orthopedic
implant biomaterials. Journal of Biomedical Materials Research Part A: 96A: 365–371.
6. Titik Nuryastuti, Henny C. van der Mei, H.J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, 2012. High frequency spontaneous deletions within the icaADBC operon of clinical
Staphylococcus epidermidis isolates, Indonesian Journal of Biotechnology, 17(2):144-153
7. Titik Nuryastuti, 2014. Current in vitro assay to determine bacterial biofilm formation of
clinical isolates, J Med Sci, Volume 46, No. 3, September: 142-152
8. Anjarwati DU, Setiawati, Mujahidah, Hapsari R, Nuryastuti T, 2014. In vitro effect of
excretion/secretion of chloroprocta sp. maggots on Staphylococcus epidermidis biofilm
and the expression level icaA gene, Indonesian J. Pharm. 25(2) : 76 – 83
9. Astuti P, Wahyono, Nuryastuti T, Purwantini I, Purwanto, 2014. Antimicrobial and
cytotoxic activities of endophytic fungi isolated from Artemisia annua L. Journal of Applied
Pharmaceutical Science. 4(10): 047-050.
10. Darussalam H, Nuryastuti T, Mursiti, Mustofa, 2014. Antimicrobial activity of bioactive
compounds isolated from Swietenia mahagoni (L) Jacq. Against Staphylococcus aureus
and Pseudomonas aeruginosa. J of Medical Sciences, 46(4):161-66
11. Titik Nuryastuti, Praseno, Mustafa M, 2015. Preliminary study of biofilm formation
properties and antibiotic susceptibility pattern of MRSA and MSSA isolates obtained in
Yogyakarta, Indonesia, Malaysian Journal of Microbiology, 11(4), 383-90.
12. Setiawati, Titik Nuryastuti, Ngatidjan, Mustofa, Jumina dan Dhina Fitriastuti, 2017, In
Vitro Antifungal Activity of (1)-N-2-Methoxybenzyl-1,10-phenanthrolinium Bromide against
Candida albicans and Its Effects on Membrane Integrity, Mycobiology, 45(1): 25-30.
13. Titik Nuryastuti, Bastiaan P. Krom, 2017. Ica-status of clinical Staphylococcus
epidermidis strains affects adhesion and aggregation: a thermodynamic analysis, Antonie
van Leuwanhoek Journal, 110:1467–1474. DOI 10.1007/s10482-017-0899-2
14. Samuel Budi, Zullies Ikawati, Iwan Dwiprahasto, Titik Nuryastuti, Antibiotics
Administration To Enhance The Success Of Therapy In Sepsis Patients, Asean J of
Pharmaceutical and Clinical Research, special issue (May 2017), DOI:
http://dx.doi.org/10.22159/ajpcr.2017.v10s2.19496
15. Ika Puspita Sari, Titik Nuryastuti, Djoko Wahyono. The study of multidrug-resistance in
neonatal intensive care unit at the Central java hospital, Asean J of Pharmaceutical and
Clinical Research, special issue (May 2017), DOI:
http://dx.doi.org/10.22159/ajpcr.2017.v10s2.194965
16. Niar Gusnaniar, Jelmer Sjollema, Ed D. Jong, Willem Woudstra, Joop de Vries, Titik
Nuryastuti, Henny C. van der Mei, Henk J. Busscher. 2017. Influence of biofilm lubricity
on shear-induced transmission of staphylococcal biofilms from stainless steel to silicone
rubber. Microb Biotechnol. 2017 Nov; 10(6): 1744–1752. doi: 10.1111/1751-7915.12798
17. Niar Gusnaniar, Jelmer Sjollema, Titik Nuryastuti, Brandon W. Peterson, Betsy van de
Belt-Gritter, Ed D. de Jong, Henny C. van der Mei & Henk J. Busscher (2017) Structural
changes in S. epidermidis biofilms after transmission between stainless steel surfaces,
Biofouling, 33:9, 712-721, DOI: 10.1080/08927014.2017.1360870
18. Niar Gusnaniar, Henny C. van der Mei, Wenwen Qu, Titik Nuryastuti, Johanna M.M.
Hooymans, Jelmer Sjollema, Henk J. Busscher. 2017. Physico-chemistry of bacterial
transmission versus adhesion. Advances in Colloid and Interface Science 250:15–24. DOI
:10.1016/j.cis.2017.11.002
19. Dwi Utami Anjarwati, Titik Nuryastuti, Ignatius Riwanto, Hendro Wahyono, 2017. Effects
of Chloroprocta sp. maggot filtrates on extracellular matrix reduction and embedded
Staphylococcus epidermidis viability, Malaysian Journal of Microbiology, 13(3), 235-243
20. Retno Wahyuningrum, Ritmaleni, Tatang Irianti, Subagus Wahyuono, Takushi Kaneko,
Titik Nuryastuti. 2017. Antituberculosis Activity of Brotowali (Tinospora crispa) Extract
and Fractions against Mycobacterium tuberculosis using Microplate Alamar Blue Assay
Method. Trad. Med. J. 22(2), p 124-130
21. Titik Nuryastuti, Setiawati Setiawati, Ngatidjan Ngatidjan, Mustofa Mustofa, Jumina
Jumina, Dhina Fitriastuti, Muhammad Idham Darussalam. 2018. Antibiofilm Activity of (1)-
N-2-Methoxybenzyl-1,10-phenanthrolinium Bromide against Candida albicans, Journal of
Medical Mycology/Journal de Mycologie Médicale, 28(2):367-373.
22. Titik Nuryastuti, 2018. Staphylococcus epidermidis: how to turn from commensal to be a
pathogen lifestyle.J Med Sci, 50(1):111-125
23. Gusnaniar, Ferdi Hizal, Chang-Hwan Choi, Jelmer Sjollema, Titik Nuryastuti, Minie
Rustema-Abbing, Rene T. Rozenbaum, Henny C. van der Mei, Henk J. Busscher, Stefan
W. Wessela. 2018. Transmission of Monospecies and Dual-Species Biofilms from Smooth
to Nanopillared Surfaces, Applied and Environmental Microbiology, 84 (15): e01035-18
24. Titik Nuryastuti, Ning Rintiswati, Praseno, Preliminary Study of Nontuberculous
Mycobacteria (NTM): Biofilm Formation, Sliding Motility, and Antibiotic Susceptibility
Pattern, Mandala of Health a Scientific Journal Vol.11, No.1, Maret 2018:10-19.
25. Gusnaniar, Ferdi Hizal, Chang-Hwan Choi, Jelmer Sjollema, Titik Nuryastuti, Minie
Rustema-Abbing, Rene T. Rozenbaum, Henny C. van der Mei, Henk J. Busscher, Stefan
W. Wessela. 2018. Transmission of Monospecies and Dual-Species Biofilms from Smooth
to Nanopillared Surfaces, Applied and Environmental Microbiology, 84 (15): e01035-18
26. Nirwati H, Hakim MS, Darma S, Mustafa M, Nuryastuti T.Detection of blaoxa genes and
identification of biofilm-producing capacity of Acinetobacter baumannii in a tertiary
teaching hospital, Klaten, Indonesia. Med J Malaysia. 2018 Oct;73(5):291-296.
27. Titik Nuryastuti, Nusaibah Umaroh, Rizka Humardewayanti Asdi, Ika Puspita Sari,
Ahmad Musthafa. 2018. Pan-drug-resistant and biofilm-producing strain of Burkholderia
pseudomallei: First report of melioidosis from a diabetic patient in Yogyakarta, Indonesia.
International Medical Case Reports Journal, 11. 319–323.
28. Titik Nuryastuti, Umaroh N, Asdie RH, Sari IP, Musthafa A.Pan-drug-resistant and
biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a
diabetic patient in Yogyakarta, Indonesia [Response to Letter].Int Med Case Rep J. 2019
May 31;12:171-172. doi: 10.2147/IMCRJ.S213150
29. Hera Nirwati, Kian Sinanjung, Fahrina Fahrunissa, Fernando Wijaya, Sarastia Napitupulu,
Vania P. Hati, Mohamad S. Hakim, Andreanita Meliala, Abu T. Aman and Titik
Nuryastuti, Biofilm formation and antibiotic resistance of Klebsiella pneumoniae isolated
 from clinical samples in a tertiary care hospital, Klaten, Indonesia. BMC Proceedings
2019, 13(Suppl 11):20 https://doi.org/10.1186/s12919-019-0176-7.
30. Andre Krislee, Chaerul Fadly, Dwi Aris Agung Nugrahaningsih, Titik Nuryastuti, Febri
Odel Nitbani, Jumina and Eti Nurwening Sholikhah. The 1-monolaurin inhibit growth and
eradicate the biofilm formed by clinical isolates of Staphylococcus epidermidis. BMC
Proceedings 2019, 13(Suppl 11):19 https://doi.org/10.1186/s12919-019-0174-9.
31. Hasyrul Hamzah, Triana Hertiani, Sylvia Utami Tunjung Pratiwi, Titik Nuryastuti. The
Inhibition Activity of Tannin on the Formation of Mono-Species and Polymicrobial Biofilm
Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida
albicans. Trad. Med. J., May-August 2019. Vol. 24(2), p 110-118
32. Hasyrul Hamzah, Triana Hertiani, Sylvia Utami Tunjung Pratiwi, Titik Nuryastuti. (2020).
Inhibitory activity and degradation of curcumin as Anti-Biofilm Polymicrobial on Catheters.
International Journal of Research in Pharmaceutical Sciences, 11(1), 830-835.
https://doi.org/10.26452/ijrps.v11i1.1902.
33. Hamzah, Hasyrul; Hertiani, Triana; Pratiwi, Sylvia; Nuryastuti, Titik. (2020). Inhibitory
activity and degradation of curcumin as Anti-Biofilm Polymicrobial on Catheters.
International Journal of Research in Pharmaceutical Sciences. 11. 830-835.
10.26452/ijrps.v11i1.1902.
34. Hertiani, Triana; Pratiwi, Sylvia; Nuryastuti, Titik; Murti, Yosi; Hamzah, Hasyrul. (2020).
The Inhibition and Degradation Activity of Demethoxycurcumin as Antibiofilm on C.
albicans ATCC 10231. Research Journal of Pharmacy and Technology. 13.
10.5958/0974-360X.2020.00075.X.
35. Mohamad S. Hakim, Luthvia Annisa, Endah Supriyati, Edwin W. Daniwijaya, Rakhmat A.
Wibowo, Eggi Arguni, Titik Nuryastuti. 2020. Current understanding of the origin,
molecular biology and continuing evolution of severe acute respiratory syndrome
coronavirus-2(SARS-CoV-2).J.Med.Sci:Vol:52(3).(SI):17-29.
http://dx.doi.org/10.19106/JMedSciSI005202202003
36. Rita Cempaka, Titik Nuryastuti, Vincent Laiman, Fara Silvia Yuliani, Ahmad Musthafa,
Hadis Pratiwi, Marcellus, Didik Setyo Heriyanto, 2020. High expression of early secretory
antigenic target 6 mRNA as a potential predictor of tuberculous lymphadenitis, Bali
Medical Journal (Bali Med J), Volume 9(3): 794-798
37. Gunadi, Wibawa H, Marcellus, Hakim MS, Daniwijaya EW, Rizki LP, Supriyati E,
Nugrahaningsih DAA, Afiahayati , Siswanto , Iskandar K, Anggorowati N, Kalim AS,
Puspitarani DA, Athollah K, Arguni E, Nuryastuti T, Wibawa T. 2020. Full- length genome
characterization and phylogenetic analysis of SARS-CoV-2 virus strains from Yogyakarta
and Central Java, Indonesia. PeerJ 8:e10575 DOI 10.7717/peerj.10575
38. Darma S, Ambara A, Aman AT, Annisa L, Nurrokhman , Nuryastuti T, et al. (2020) High
frequency of azole resistant Candida spp. colonization among presumptive multidrug
resistant tuberculosis (MDR-TB) patients. PLoS ONE 15(11): e0242542.
https://doi.org/10.1371/ journal.pone.0242542
39. Rizqi Nurul Khasanah, Ika Puspitasari, Titik Nuryastuti, Nunung Yuniarti, Prevalence of
MultiDrug-Resistent Klebsiella pneumonia and Evaluation of Suitability Empirics
Antibiotics Based on Pharmacokinetic Prediction Value to Clinical Outcome in RSUP Dr.
Soeradji Tirtonegoro Klaten, Majalah Farmaseutik Vol. 16(1): 27-33
40. Rina Triasih, Amalia Setyati, Dwikisworo Setyowireni, Titik Nuryastuti, Rachma Dewi
Isnaini Putri, Emi Rusdiyanti. 2020. Use of Xpert MTB/RIF for diagnosis of pediatric
tuberculosis in Indonesia. Paediatr Indones. 2020;60:198-204; DOI: 10.14238/
pi60.4.2020.198-204
Scientific Conference
1. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, Spontaneous deletion of ica operon in clinical isolates S. epidermidis, Oral
Presentation, the International Biomedical Engineering Conference, Institute Technology
of Bandung, 13-15 November 2006, Bandung, Indonesia.
2. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, Spontaneous deletion of ica genes in clinical isolates S. epidermidis, Poster
Presentation the Annual National Scientific Meeting of Indonesian Clinical Microbiology
Association, 18-19 November 2006, Malang, Indonesia.
3. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, Regulation of ica expression and biofilm formation in clinical isolates S,
epidermidis, Poster Presentation. Biomedical, Material Science and Application Meeting,
Department of Biomedical Engineering, Rijks Universiteit Groningen, 13 April 2007.
Schiermonikoog, The Netherlands.
4. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, Spontaneous deletions within the icaADBC operon of clinical Staphylococcus
epidermidis isolates; the possible role of LexA in phenotypic variations, Poster
Presentation, The 162nd Society of General Microbiology (SGM) Meeting, 31 Maret-3 April
2008, Edinburgh, Scotland, United Kingdom.
5. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, Effect of cinnamon oil on icaA expression and biofilm formation by Staphylococcus
epidermidis, Oral Presentation. Biomedical, Material Science and Application Meeting,
Department of Biomedical Engineering, Rijks Universiteit Groningen, 21 April 2008.
Schiermonikoog, The Netherlands.
6. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, recA mediated spontaneous deletions of the icaADBC operon of clinical
Staphylococus epidermidis isolates : a new mechanism of phenotypic variations, Poster
Presentation, the 2nd Malaysia Tissue Engineering & Regenerative Medicine Scientific
Meeting, 22-23 July 2008, Kuala Lumpur Malaysia.
7. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, recA mediated spontaneous deletions of the icaADBC operon of clinical
Staphylococus epidermidis isolates : a new mechanism of phenotypic variations, Poster
Presentation, the 13th International Symposium on Staphylococci and Staphylococcal
Infection, 7-10 September 2008, Cairns, Australia.
8. Dewi Santosaningsih, H.A. Verbreugh, Kurniawati A, Titik Nuryastuti. The first multicenter
study of methicillin resistance Staphylococus aureus in Indonesian hospitals. Poster
Presentation. the 13th International Symposium on Staphylococci and Staphylococcal
Infection, 7-10 September 2008, Cairns, Australia.
9. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, Iravati, S, A.T.Aman, Bastiaan P.
Krom, Antimicrobial activity of cinnamon oil against clinical S. epidermidis strains in
planktonic and biofilm mode of growth, Oral Presentation, the Annual National Scientific
Meeting of Indonesian Clinical Microbiology Association, 13-15 November 2009,
Yogyakarta, Indonesia.
10. Titik Nuryastuti, Henny C. van der Mei, H,J. Busscher, R. Kuijer, A.T.Aman, Bastiaan P.
Krom, The role of genotypic changes on the development of phenotypic variations in
clinical isolates of Staphylococcus epidermidis, Poster Presentation, the Annual National
Scientific Meeting of Indonesian Clinical Microbiology Association, 6-8 Agustus 2010,
Bandung, Indonesia.
11. Titik Nuryastuti, Gusnaniar, Eka L. Hapsari. 2013. The pravalence of CLABSI in NICU,
Sardjito Hospital, Yogyakarta. Maternal and Child Health Summer School 2013
 collaborated with IGHEP (Indonesian Germany Health Education Partnership), Nov 2013.
Mulawarman University, Samarinda, East Kalimantan and Udayana University, Denpasar,
Bali.
12. Titik Nuryastuti, Gusnaniar, Eka L. Hapsari. 2014. Biofilm Formation of Microorganism
causing Central line-associated Blood Stream Infections (CLABSI). Maternal and Child
Health Summer School 2014 collaborated with IGHEP (Indonesian Germany Health
Education Partnership), Sept 2014. Cendrawasih University, Jayapura.
13. Titik Nuryastuti, Ning Rintiswati. 2014. Rapid growing mycobacterium:
its biofilm formation and antibiotic susceptibility. Tropical Medicine Summer School 2014
collaborated with IGHEP (Indonesian Germany Health Education Partnership), Sept 2014.
Syah Kuala University, Banda Aceh.
14. Titik Nuryastuti, Antibiofilm effect of 1,10 Phenantroline against Candida albicans,
Eurobiofilm Meeting, Amsterdam, The Netherlands, 19-23 September 2017.
15. Titik Nuryastuti, Ning Rintiswati, Sumardi, Heni Retno Wulan, Salsabilla Hasna Mutiara
Rizki, Stefanie Juergens, Yuli Mawarti, Tri Wibawa, Alex Prasudi, Evaluation of AFB and
Xpert®MTB/RIF for diagnosis of tuberculosis in Yogyakarta, Indonesia, 22nd Bangkok
International Symposium on HIV Medicine 2020, January 15-17, 2020.