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    Benedict's Reagent Is The Solution Used in Benedict's Test To Detect Simple Sugars Such As

    Uploaded by

    Cristoper Bodiongan
    This document provides instructions for nurses on how to properly perform Benedict's test and a urine test for albumin. Benedict's test is used to detect reducing sugars like glucose in urine. The key steps are to add Benedict's reagent to urine in a test tube, heat the solution, and observe any color changes, which can indicate the percentage of sugar present. A urine albumin test checks for excess protein in urine, which may indicate kidney damage. For this test, urine is heated in a test tube and acetic acid is added to confirm if cloudiness persists, meaning the result would be positive for albumin.

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    Benedict's Reagent Is The Solution Used in Benedict's Test To Detect Simple Sugars Such As

    Uploaded by

    Cristoper Bodiongan
    86 views2 pages
    This document provides instructions for nurses on how to properly perform Benedict's test and a urine test for albumin. Benedict's test is used to detect reducing sugars like glucose in urine. The key steps are to add Benedict's reagent to urine in a test tube, heat the solution, and observe any color changes, which can indicate the percentage of sugar present. A urine albumin test checks for excess protein in urine, which may indicate kidney damage. For this test, urine is heated in a test tube and acetic acid is added to confirm if cloudiness persists, meaning the result would be positive for albumin.

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    benedict albumin

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    This document provides instructions for nurses on how to properly perform Benedict's test and a urine test for albumin. Benedict's test is used to detect reducing sugars like glucose in urine. The key steps are to add Benedict's reagent to urine in a test tube, heat the solution, and observe any color changes, which can indicate the percentage of sugar present. A urine albumin test checks for excess protein in urine, which may indicate kidney damage. For this test, urine is heated in a test tube and acetic acid is added to confirm if cloudiness persists, meaning the result would be positive for albumin.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    86 views2 pages

    Benedict's Reagent Is The Solution Used in Benedict's Test To Detect Simple Sugars Such As

    Uploaded by

    Cristoper Bodiongan
    This document provides instructions for nurses on how to properly perform Benedict's test and a urine test for albumin. Benedict's test is used to detect reducing sugars like glucose in urine. The key steps are to add Benedict's reagent to urine in a test tube, heat the solution, and observe any color changes, which can indicate the percentage of sugar present. A urine albumin test checks for excess protein in urine, which may indicate kidney damage. For this test, urine is heated in a test tube and acetic acid is added to confirm if cloudiness persists, meaning the result would be positive for albumin.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Good noon, Maam Pero. Praised be Jesus and Mary! I am Henry D.

    Solatorio Jr and at this point I


    am going to demonstrate how nurses should properly and effectively perform the Benedict’s Test.

    As a general understanding, Benedict’s test is a simple chemistry test used to detect reducing
    sugars. Reducing sugars are carbohydrates having free aldehyde or ketone functional group in its
    molecular structure. These include monosaccharides like glucose and fructose and disaccharides
    like lactose and maltose.

    Benedict’s reagent is the solution used in Benedict’s test to detect simple sugars such as
    glucose. It is a bright blue solution prepared by mixing copper sulfate pentahydrate sodium citrate,
    and sodium carbonate in distilled water

    In conducting the test, it is important to make sure all equipment are complete and of good
    condition. These include the following, Urine sample, Benedict’s reagent, Test tube, Alcohol lamp,
    dropper.

    After making sure the equipment is ready for use, we are now going to proceed with the
    procedure.

    First and foremost,


    1. In a test tube pour a 5ml Benedict’s reagent.

    After doing this,


    2. Mix in the same test tube 8 drops of urine..

    After which,
    3. Heat it under the alcohol lamp for 2 mins And allow it to cool down

    Upon doing this, Observe for color change in the solution of test tubes or precipitate formation and
    4. Compare the results.

    If the color upon boiling is changed into green, then there would be 0.1 to 0.5 percent sugar in
    solution.
    If it changes color to yellow, then 0.5 to 1 percent sugar is present.
    If it changes to orange, then it means that 1 to 1.5 percent sugar is present.
    If color changes to red,then 1.5 to 2.0 percent sugar is present.
    And if color changes to brick red, it means that more than 2 percent sugar is present in solution.
    Another test involving urine is the urine test for albumin. An albumin test checks urine for a protein
    called albumin. Albumin is normally found in the blood and filtered by the kidneys. When the
    kidneys are working as they should, there may be a very small amount of albumin in the urine. But
    when the kidneys are damaged, abnormal amounts of albumin leak into the urine.

    Like the benedict’s test, it is also essential to prepare the materials needed and it includes: Urine
    sample, Acetic acid solution, Dropper, Test tube and Test tube holder.

    After the materials are prepared, we are now going to have the procedure:

    First things first, you are going to


    1. Fill ¾ of the test tube with urine.

    Up next, you need to


    2. Heat the upper portion of the test tube with urine without shaking to be able to compare
    results with bottom part of the test tube.

    It is important to note that


    3. If there is no change in the heated portion, result is NEGATIVE and not necessary to add
    the acetic acid.
    And lastly,
    4. If cloudiness appears at the heated portion, add 2-3 drops of acetic acid and heat again to
    rule out presence of phosphates. If cloudiness disappears, result is NEGATIVE. But if it
    persists in spite of the addition of acetic acid or even deepend, the result is POSITIVE.

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