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    Enzymy

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    DrShailesh Sawale
    Enzymes are highly selective stereospecific perform complicated reactions: neutral pH ambient temperatures atmospheric pressure aqueous environment. A key aspect of any process is the capability to re-use the enzyme. The use of resins to immobilize enzymes has found uses in many different industries such as: Food Sugar Pharmaceutical Chemical processing Waste treatment 9.

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    Download as PDF, TXT or read online from Scribd

    Enzymy

    Uploaded by

    DrShailesh Sawale
    96 views11 pages
    Enzymes are highly selective stereospecific perform complicated reactions: neutral pH ambient temperatures atmospheric pressure aqueous environment. A key aspect of any process is the capability to re-use the enzyme. The use of resins to immobilize enzymes has found uses in many different industries such as: Food Sugar Pharmaceutical Chemical processing Waste treatment 9.

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    enzymy

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    Enzymes are highly selective stereospecific perform complicated reactions: neutral pH ambient temperatures atmospheric pressure aqueous environment. A key aspect of any process is the capability to re-use the enzyme. The use of resins to immobilize enzymes has found uses in many different industries such as: Food Sugar Pharmaceutical Chemical processing Waste treatment 9.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    96 views11 pages

    Enzymy

    Uploaded by

    DrShailesh Sawale
    Enzymes are highly selective stereospecific perform complicated reactions: neutral pH ambient temperatures atmospheric pressure aqueous environment. A key aspect of any process is the capability to re-use the enzyme. The use of resins to immobilize enzymes has found uses in many different industries such as: Food Sugar Pharmaceutical Chemical processing Waste treatment 9.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 11

    Together, building tomorrow’s solutions

    ©2001 Rohm and Haas Company - 10/01


    Regarded as Nature’s catalysts,
    enzymes are:

    highly selective
    stereospecific
    perform complicated reactions:
    neutral pH
    ambient temperatures
    atmospheric pressure
    aqueous environment.

    A key aspect of any process is the


    capability to re-use the enzyme.
    Original product Enzyme reaction

    New product Impurities Original product

    Crystallisation Recycle
    Advantages
    Easy separation from reaction mixture
    Re-use of enzyme for many reaction cycles
    Lower cost of enzyme
    Selective reactions
    Increased stability of enzyme
    Known engineering technology

    Disadvantage
    Lower activity than free enzyme
    General observations

    Macroreticular structure
    Weakly ionic or non functionalized resins
    Relatively hydrophilic surface : acrylic and phenolic
    resins
    Particle size important for kinetic reasons

    Apart from the first point, there are exceptions to


    each of the above.
    Methods of fixation

    Simple adsorption onto a buffered resin.


    Adsorption onto a buffered resins followed by
    treatment with glutaraldehyde.
    Ionic bonding ( with H+ or OH- form resins).
    Covalent bonding via -NH2 groups.
    Via metals such as Fe, Ni, Al etc.
    Engineering Technology

    Fixed
    FixedBed
    Bed Fluidised
    FluidisedBed
    Bed
    The use of resins to immobilize enzymes have
    found uses in many different industries such as:

    Food
    Sugar
    Pharmaceutical
    Chemical processing
    Waste treatment
    • Duolite XAD761 ß-amylase, ß-Galactosidase,
    Lactase, Papain, Chymotrypsin,
    Glucoseoxidase, Lipase.

    • Amberlite IRC50 Lysozyme (recovery),


    Cytochrome C, Acylase

    • Amberlite XAD7 HP Thermolysin, Penicillin


    acylase, Lipase, ß-amylase
    • Amberlite IRA938*
    Pepsin, Pepsinogen

    • Duolite A568
    Glucose Isomerase, Lipases

    • Duolite A7
    Trypsin, Aspartase, Aminocylase,
    RNase, Lactase.

    * Not Currently Manufactured


    Rohm and Haas Company makes no warranties, either expressed or implied as to the accuracy of
    appropriateness of the data in this document and expressly excludes any liability upon Rohm and Haas
    arising out of its use. We recommend that the prospective users determine for themselves the suitability of
    Rohm and Haas materials and suggestions for any use prior to their adoption.
    Suggestions for uses of our products or the inclusion of descriptive material from patents and the citation of
    specific patents in this publication should not be understood as recommending the use of our products in
    violation of any patent or as permission or license to use any patents of the Rohm and Haas Company.
    Material Safety Data Sheets outlining the hazards and handling methods for our products are available on
    request.
    Amberlite and Duolite are trademarks of Rohm and Haas Company, Philadephia.
    The present document is copyrighted by Rohm and Haas and shall not be reproduced without a written
    authorisation of Rohm and Haas Company.

    ©2001 Rohm and Haas Company

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