Steam Distillation of Orange Oil: "Isoprene" Units
Steam Distillation of Orange Oil: "Isoprene" Units
Steam Distillation of Orange Oil: "Isoprene" Units
Partners_______________________
_______________________
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Section (Circle) M Tu W Th Date_______________________
Materials: 3 oranges, boiling chips, NaCl, ice, blender, grater, 50 mL buret, distillation
apparatus, screw-top vial, ice, stirring rod, rinse bottle, vacuum grease, aluminum foil,
cleaning brushes, detergent (Dawn)
Purpose: In this laboratory activity you will separate orange oil from orange peel by the
technique of steam distillation.
Terpenes and terpenoids are the primary constituents of the essential oils of
many types of plants and flowers. Essential oils are used widely as natural flavor
additives for food, as fragrances in perfumery, in aromatherapy, and in traditional and
alternative medicines. Synthetic variations and derivatives of natural terpenes and
terpenoids also greatly expand the variety of aromas used in perfumery and flavors used
in food additives. The building block of all terpenes is an isoprene unit. A limonene
molecule contains two isoprene units.
or
“Isoprene” Units
The terpene D-limonene is the major chemical component in orange oil and has
a variety of uses. As the main odor constitutent of the citrus family, D – limonene can
be used as an additive to impart an orange-like fragrance or flavor to foods, toiletries,
and cleaning products. Pure limonene is used as a solvent to replace other solvents
such as toluene, mineral spirits, turpentine, acetone, etc. Flea and tick shampoos also
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contain D-limonene as do some insecticides. Limonene is an irritant to the skin and
eyes.
D – limonene L – limonene
There are two isomers (same chemical formula but different structure) of
limonene, D and L limonene. A quick glance at the structural representations of
limonene above reveals that both of these isomers have the same connection of
atoms. How can they be isomers? D and L limonene are stereoisomers, specifically,
enantiomers or optical isomers. Stereoisomers are structures that differ only in the
spatial arrangement of atoms.
Safety: Be sure that your distillation apparatus is secure before beginning the
distillation. Have your instructor check your set-up before you begin. Limonene is a skin
and eye irritant.
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Procedure:
1. Peel three or four medium oranges and puree the peel with about 100
mL of distilled water in a blender or food processor. You do not want
the fruit pulp. The limonene is concentrated in the peel.
2. Transfer the peel puree to a 500 mL round bottom flask using a wide-
mouth funnel and a stirring rod. You may have to rinse the peel puree
into the flask using some DI water. Add enough water of to the
mixture in the flask to 2/3's the capacity the flask. Don’t add too much
water as you do not want the orange peels and water shooting up into
the Claisen while boiling!
5. Look over the set-up in the front of the room for the placement of the
spring clamps. Also notice that there is one addition clamp in the set-up
at the receiving flask. Your heat source will be a hot plate with an
aluminum block.
7. Wrap the claisen and stillhead with aluminum foil. This will keep the
steam hot and allow it to keep traveling to the condenser.
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9. Turn on the condenser water.
10. Turn on the hot plate to the “8” setting. It will take about 10 – 15
minutes for your orange peel puree to boil.
11. What is the temperature at the still head when your mixture starts to
boil? ____________
The idea is that you want a vigorous enough boiling cause refluxing of
the mixture but not so vigorous that it urps into the stillhead!
12. What is the temperature at the still head when you collect your first
drop of distillate? ___________
13. As the mixture boils and distills, you will be losing water from the
distillation mixture. As the level drops in the flask, add small volumes
of water via the separatory funnel. It is important to watch the water
level because of the high concentration of sugar in oranges. If the
water level gets too low, the sugar will carmelize and burn. It is better
to add small amounts of water so that the temperature doesn’t drop
drastically.
15. Turn off the hot plate. Remove the aluminum foil. We will recycle the
foil for the next lab. Wait 15 minutes for the system to begin to cool.
The distillation will continue during this time.
16. Very carefully remove the receiving flask from the set-up and place it in
a beaker. Place a beaker under the vacuum adapter to catch any
drips.
17. Add about a half of a gram of NaCl to the distillate. This raises the
ionic strength of the water and increases the separation of the two
layers. You should be able to observe droplets or a film of the product,
limonene, on the surface of the distillate.
19. Pour the contents of the flask into the burette. (If you have much more
than 50 mL you’ll need to do this in batches.) Allow the liquid to settle;
all the orange oil should rise to the top of the burette.
20. Carefully read buret to determine the volume of orange oil collected.
Ask your instructor for help if you are unsure. About how many mL of
orange oil did you collect? __________
21. Slowly drain off the water until it is all drained out. This aqueous
portion of the distillate is called the hydrosol. Save some of the
hydrosol in a labeled vial.
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22. Drain the orange oil into a clean, dry, pre-weighed labeled screw-top
vial. Reweigh the vial to determine the amount of product. Save this oil
for the soap-making lab later on in the semester.
23. Now it’s time for clean-up! The orange peel puree can go down the
drain. If you can, try to recover the boiling chips.
24. Use the brushes and detergent to clean your distillation set-up.
25. The buret can be cleaned with a few rinses with soapy water, then tap
water, and finally DI water. Fill the buret up with DI water and replace
the cork.
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Lab Report Name _______________________
Steam Distillation of Orange Oil
Chem 306 Partners_______________________
_______________________
_______________________
Data
Show set-up and work for density calculation in the space below: Don’t forget your units
and sig figs! Box your final answer.
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9. Repeat the above wafting procedure for the hydrosol.
2. Draw the expanded structure of D – limonene. Circle the two isoprene units in
limonene.
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5. What type of isomerism is exhibited by these two compounds?
6. Predict the hydration product of limonene if two molecules of water are added
to a limonene molecule.
7. Do you think that limonene is flammable? Dip a metal spatula into your
limonene and gently pass it through a flame. Report your observations.
C. Limonene as a Hydrocarbon
The activities in this lab illustrate some general properties of
hydrocarbons. State two of these properties of hydrocarbons.