Ei Rms1000-Brochure Web
Ei Rms1000-Brochure Web
Ei Rms1000-Brochure Web
RAMAN MICROSCOPE
RMS1000
EDINBURGH INSTRUMENTS
MOLECULAR SPECTROSCOPY SINCE 1971 CIRCLE Photoluminescence CIRCLE Raman CIRCLE UV-Vis CIRCLE Transient Absorption
KEY FEATURES
Integrated and External Lasers – up to 5 internal computer-controlled
lasers for ease of use and reduced footprint. External laser integration
for advanced Raman and Fluorescence measurements
SEMICONDUCTORS: 325 nm
RECOMMENDED
LASER SOURCES UV Raman analysis of GaN
materials provides both Raman and
photoluminescence data which can
GENERAL PURPOSE be used to detect defects in the
532 nm | 638 nm | 785 nm material. This facilitates monitoring
and improvement of manufacturing
processes and device performance.
SEMICONDUCTORS
325 nm | 473 nm | 532 nm
NANOMATERIALS: 532 nm
PHARMACEUTICALS
532 nm | 638 nm | 785 nm Confocal Raman is ideal for
characterising the electronic structure of
graphene. Graphene has 2 major bands
NANOMATERIALS (1580 cm-1 and 2680 cm-1). The ratio of
532 nm | 638 nm | 785 nm these peaks changes in dependence of
the number of layers.
BIOSCIENCES
785 nm | 830 nm | 1064 nm
BIOSCIENCES: 785 nm
POLYMERS
Raman spectrum taken from a grain
532 nm | 638 nm | 785 nm
of buttercup pollen, showing Raman
peaks from carotenoid species as well
GEOLOGY
as proteins and amino acids which can
be used to identify species. 785 nm
532 nm | 785 nm
excitation was chosen to minimise
fluorescence background.
2
RAMAN SCATTER PATH – SPECTROGRAPH CHOICE
MATCH THE FOCAL
LENGTH OF YOUR
The RMS1000 features a versatile and configurable Raman SPECTROGRAPH TO
scatter path with all hardware options fully computer THE REQUIREMENTS
OF YOUR DEMANDING
controlled. Two spectrograph versions are available for RESEARCH.
standard research and the most demanding applications.
3
CONFOCAL RAMAN MICROSCOPY
DO NOT GO WITH
A COMPROMISED
Due to its true confocal design, the RMS1000 offers OPTICAL DESIGN!
excellent 2D mapping, depth profiling, and 3D mapping GO FOR TRULY
CONFOCAL!
capabilities and provides close to diffraction-limited
spatial resolution.
to Spectrograph
True confocal design with a software controlled
Confocal
confocal pinhole turret Pinhole
Focal
Plane
detected radiation rejected radiation Sample
IMPROVEMENT OF
SPATIAL RESOLUTION 14 14
12 12
The confocal pinhole design significantly
enhances the contrast of 2D Raman maps 10 10
due to better spatial selection and better
8 8
discrimination from unwanted signal
Y / µm
Y / µm
DEPTH PROFILING
70 70
A confocal pinhole design is an essential
requirement if the ‘Z-dimension’ of the 60 60
Z / µm
enhancement of contrast in the ‘Z’ dimension. Raman Shift / cm-1 Raman Shift / cm-1
4
RAMAN MAPPING
BEST PERFORMANCE
RAMAN MAPPING:
The RMS1000 comes with Raman Mapping that CONFOCAL, WITH
has been central to the design from concept to HIGH SPEED AND HIGH
DATA ACCURACY.
completion. Raman Mapping truly adds a different
dimension to this high-end Raman Microscope.
Mapping the Raman spectra within a sample space provides Effective Raman mapping sets special requirements for
previously unavailable information about the chemical and hardware and software. The fastest and most sensitive array
physical differences across a sample. This can confirm the detectors, EMCCDs, should be considered, together with
identity and presence of specific components, and reveals a fast scan stage and proven and tested software that can
their location and distribution within the sample. acquire and process vast data sets with high accuracy and
in the shortest possible time.
The RMS1000 generates Raman maps using a motorised
stage for sample scanning, which permits finely controlled 3D RAMAN MAPPING
movement in the X, Y and Z planes. Line scans, plane maps,
and volume maps can be created. Auto-focus options help
tracking of uneven surfaces and sample planes.
Material sciences
Investigate how structure, stress and strain vary
across a sample
Pharmaceuticals
Identify ingredients and analyse their distribution
in drug development and drug production 4
2D RAMAN MAPPING
5
0 100 0 20
µm µm 6
Layers 1, 6
Layer 2
0 20 0 10 Layers 3, 5
µm µm
Layer 4
1 I nverted microscope
Standard spectrograph
6
FLEXIBILITY IN RESOLUTION
FOR ADVANCED RAMAN RESEARCH
3 I nverted microscope
Long focal length spectrograph
4 U
pright microscope
Long focal length spectrograph,
laser safety enclosure
7
RMS1000 PERFORMANCE
SPECTRAL RESOLUTION
The optical path of the RMS1000 has been optimised with narrow-
band lasers, superior flat-field optics, a choice of two high quality
spectrographs, and high performance pixel matched cameras; all
to achieve the best spectral resolution for the user. High spectral
resolution over a broad range is possible by stitching together
spectra in a single measurement process within Ramacle software.
SPATIAL RESOLUTION
The wide choice of objectives and the adjustable pinhole for spatial
filtering provides excellent flexibility and allows spatial resolution to
be optimised depending on sample requirements.
SENSITIVITY
Spectral correction
FEATURES INCLUDED
UPLOAD WITH UPGRADES
9
SPECIAL FEATURES AND OPTIONS
AUTOFOCUS
0 100
The autofocus capability is shown on the example of a chalcopyrite rock. The top
picture shows a brightfield microscopic image of the rock shown in the insert. It is
evident that only a small fraction of the overall image is in focus.
The picture in the middle shows the ‘flattened’ image of the same surface as above
with autofocus correction. All surface points are sharp in focus.
The bottom picture shows a 3D representation of the same surface. The Z-coordinates
for each surface point are used when Raman mapping to this surface is applied. The
Raman map (insert) reveals structural changes across the surface.
TEMPERATURE MEASUREMENTS
Ramacle provides the option to view the spectra at the Ultimately, the RMS1000 can be configured with pulsed
wavelength scale, and spectrally corrected, requirements that lasers, TCSPC or MCS* timing electronics, and single
are typical for fluorescence and photoluminescence, but less photon counting detectors for fluorescence lifetime
common in Raman spectroscopy. measurements or decay measurements in the nanosecond
time scale. Spatial mapping of this type of measurements
The picture below shows security features of a section of a will result in FLIM or PLIM images, respectively.
banknote. Brightfield image (left), photoluminescence map
(right) and photoluminescence spectra of the two security
dyes (middle).
0 20
0 40
µm
µm
Brightfield image of a banknote security feature (left); emission spectra of the two security inks (centre) Photoluminescence map of the security feature (right)
The picture (right) displays the wide field with the selected laser
spot for excitation. The insert shows the fluorescence lifetime
measurement (red) together with the instrumental response
function (blue) which was measured by replacing the sample
with an ultra-fast decaying fluorophore (4-DASPI).
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UPGRADE OPTIONS
LASERS
The RMS1000 is built with flexibility in mind. A choice of excitation lasers
(internal or external to the system) and associated laser rejection filters
(both edge and notch) are available. Additionally picosecond pulsed diode
lasers (EPL series) can be added if fluorescence measurements are required.
SPECTROGRAPHS
The RMS1000 can be fitted with both standard and enhanced focal length
spectrographs. This allows the system to be configured for ultimate
spectral resolution, maximum optical throughput, low wavelength and
ultraviolet spectral ranges, or for highly sensitive measurements.
DETECTORS
A choice of CCD, EMCCD and InGaAs detectors are available with a maximum
of 4 detectors being integrated per system. For fluorescence lifetime
measurements, single photon counting detectors can also be integrated.
MICROSCOPE ACCESSORIES
The RMS1000 uses either an upright or inverted microscope platform
which is compatible with all standard microscopy applications.
Brightfield, darkfield, polarised light, differential interference contrast
(DIC) and fluorescence accessories are all available. A large choice
of high-quality objectives, high-performance visualisation cameras,
collimators and illuminators can be added to the microscope at any time.
SAMPLE STAGES
Manual or motorised stages are available. The motorised stage
allows automated XYZ Raman and fluorescence maps to be obtained
and generated through Ramacle. Autofocus and heating/cooling of
samples is also available.
12
SPECIFICATIONS – RMS1000
LASERS Up to 5 integrated narrow-band lasers: 532 nm, 638 nm, 785 nm typically used
Additional lasers from UV to NIR are available. External lasers can be integrated
SPECTRAL RESOLUTION From <0.1 cm-1 (depending on grating, laser and CCD selection)
SPATIAL RESOLUTION XY (lateral), Z (axial) 0.25 µm, <1 µm (depending on laser and microscope objective)
DETECTORS CCD Detector High sensitivity ultra low noise spectroscopy CCDs
Optional Second Detector EMCCD detector, 1600 x 200 pixels, TE-cooled -100oC (fast response time)
Lifetime With picosecond pulsed lasers, TCSPC electronics, fast photon counting detectors
LASER SAFETY Without Laser Enclosure Class 3B (depending on external laser source)
13
EDINBURGH
INSTRUMENTS
2 Bain Square,
Kirkton Campus,
Livingston, EH54 7DQ
United Kingdom
sales@edinst.com
U.S. OFFICE
CONTACT:
ussales@edinst.com
Customer support is
available worldwide
MANUFACTURED
WITH PRIDE IN THE
UNITED KINGDOM
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Stg21 / 10.2020