Name: Rounak Barnwal Class: Xii A Roll No: SESSION:2022-2023

TOPIC: TO STUDY THE PRESENCE OF OXALATE ION
IN GUAVA FRUIT AT DIFFERENT STAGES OF
RIPENING
NAME: ROUNAK BARNWAL

CLASS: XII A
ROLL NO:
SESSION:2022-2023
CERTIFICATE
This is to verify that ROUNAK
BARNWAL a student of class XII A
has successfully completed the
Chemistry Investigatory Project on
the topic “TO STUDY THE
PRESENCE OF OXALATE ION IN
GUAVA FRUIT AT DIFFERENT
STAGES IF RIPENING” under the
guidance of Mr. Subhadip Sil
(chemistry teacher) during the year
2022-2023 in fulfillment of chemistry
practical examination conducted by
AISSCE, NEW DELHI.
INITIAL OF EXTERNAL EXAMINER INITIAL OF EXTERNAL EXAMINER

ACKNOWLEDGEMENT
I would like to express my special gratidude to my teacher
Mr. Subhadip Sil sir as well as our principal who gave me
the opportunity to do the project on the topic “TO STUDY
THE PRESENCE OF OXALATE ION IN GUAVA FRUIT AT
DIFFERENT STAGES IF RIPENING” .
This helped me to do some research and I came to know
about so many things.
Secondly, I would like to thank my family and my friends
who helped me to complete the project within the limited
time frame. Above all I would like to thank god for his
blessings and love.
INDEX
1) EXPERIMENT :- “TO STUDY THE
PRESENCE OF OXALATE ION IN GUAVA FRUIT
AT DIFFERENT STAGES IF RIPENING”
AIM
 APPARATUS
 MATERIAL REQUIRED
 THEORY
 PROCEDURE
 OBSERVATION
 CALCULATION
 RESULT
2) BIBLIOGRAPHY
AIM:- To study the presence of oxalate
ions in guava fruit at different stages of
ripening.
APPARATUS:- 100 ml measuring flask ,
beaker , burette, pipette titration flask ,
funnel , pestle, mortar , weight box ,and
filter paper.
MATeRIAlS ReqUIRed:- (i) Guava fruit at
different stages of ripening. (ii) 0.001(M)
KMnO4 solution . (iii) Conc. H2SO4
THeORY:- Guava fruit contains oxalate ions
in addition to vitamin C oxalate ions are
extracted by boiling the fruit pulp with dil.
H2SO4 . The oxalte ions are volumetrically
by titrating the solution against a standard .
PROcedURe:-
1) Weigh 50.0gm of fresh guava fruit and
grind it to a pine pulp in a mortar with
a pestle.
2) Transfer the crushed pulp to 250 ml of
beaker and boil with dil. H2SO4 .
3) Cool and filter the contents in a 100 ml
measuring flask, take the volume
exactly 100 ml by adding distilled
water.
4) Pipette out 20 ml of guava solution in
a titration flask and add 20 ml of dil.
H2SO4 to it . Heat the mixture to 60o C
- 70o C down the 0.001(M) KMnO4
solution from the burette to fall into
the titration flask, little at a time with
constant shaking till the endpoint is
reached. The end point is the
appearance of permanent pink colour.
5) Repeat the experiment to take a set of
atleast 3 concordant reading.
6) Repeat the experiment by taking 50gm
of guava fruit at different stages of
ripening.
7) Read the observation.
ObSeRvATIOn:-
Weight of guava fruit each time =50.0gm
Volume of guava fruit taken each
time=20.0ml
Molarity of KMnO4 solution=0.001(M)
Type of guava Initial Reading Final Reading Volume of Concordant
fruit KMnO4 value of KMnO4
(i) Fresh fruit 150 ml 18 ml 132 ml 135.05 ml
(ii) one day old 150 ml 13 ml 137 ml 135.05 ml
fruit
(iii) two day old 150 ml 13 ml 137 ml 135.05 ml
fruit
(iv)three day 150 ml 10.8 ml 139.2 ml 135.05 ml
old fruit
cAlcUlATIOnS
Step 1 :-
To calculate the molarity of oxalate ions.
Let the volume of KMnO4 solution used =V1
Applying molarity equation
M1V1(KMnO4)/M2V2(guava fruit)=2/5
M2=5(M1V1)/2(V2)=(5*0.001*V1)/(2*20)=V1/8000
mol/litre
I. One day old fruit = M2 = 5(M1V1)/2(V2)
= 137/8000 mol/litre = 0.017 mol/litre.
II. Two day old fruit = M2 = 5(M1V1)/2(V2)
III. Three day old fruit = M2 = 5(M1V1)/2(V2)
= 139.2/8000 mol/litre = 0.0175 mol/litre.
IV. Fresh Guava fruit = M2 = 5(M1V1)/2(V2)
Step 2 :-
To calculate the strength of oxalate ions
in fresh guava fruit = Molarity * molar
mass of oxalate ions
 Fresh Guava fruit= Molarity * molar
mass of oxalate ions=132*88/8000
=1.452 gm/lt
 One day old fruit One day old fruit=
Molarity * molar mass of oxalate
ions=137*88/8000
=1.507 gm/lt
 Two day old fruit= Molarity * molar
mass of oxalate ions=137*88/8000
=1.507 gm/lt
 Three day old fruit= Molarity * molar
mass of oxalate ions=139.2*88/8000
=1.5312 gm/lt
RESULT
TYPES MOLARITY(M) STRENGTH
OF
GUAVA
FRUIT
One 137/8000 137*88/8000

day mol/litre = =1.507 gm/lt
old 0.017
fruit mol/litre
Two 137/8000 137*88/8000
old 0.017
fruit mol/litre
Three 139.2/8000 139.2*88/8000
old 0.0175
fruit mol/litre
Fresh 132/8000 132*88/8000
guava mol/litre = =1.452 gm/lt
fruit 0.0165
mol/litre
bIblIOgRAPHY
 COLLECTED INFORMATION FROM THE
INTERNET.
 www.google.com
 HELP FROM TEACHER AND LIBRARY

Name: Rounak Barnwal Class: Xii A Roll No: SESSION:2022-2023

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Name: Rounak Barnwal Class: Xii A Roll No: SESSION:2022-2023

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TOPIC: TO STUDY THE PRESENCE OF OXALATE ION

IN GUAVA FRUIT AT DIFFERENT STAGES OF

NAME: ROUNAK BARNWAL

INITIAL OF EXTERNAL EXAMINER INITIAL OF EXTERNAL EXAMINER

One 137/8000 137*88/8000

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