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White Paper: Biosafety and Blood Borne Pathogen Safety in The Laboratory

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White Paper: Biosafety and Blood Borne

Pathogen Safety in the Laboratory

Author: Danielle DeLucy

Owner, ASA Training & Quality Consulting, LLC

If you work in a laboratory exposed to viruses or bacteria that are biological hazards and
are searching for a better way to manage your Biosafety program, it is in your best interest
to set up a successful management policy. First and foremost, we need to discuss the
principles of biosafety. The purpose of containment is to protect lab staff from exposure to
hazardous biological agents such as, Bacteria, viruses, fungi, parasites, Recombinant DNA ,
Potentially dangerous cell lines, Transgenic animals or plants, or Biological toxins to name
a few. In addition, it is also important to guard against the release of these biohazard
materials. There are three elements of containment:

 Facility design (Engineering)

 Lab practice and techniques (Administrative)

 Personal Protective Equipment (PPE)

The extent of containment depends on the level of risk and the nature of biological agent.

Primary barriers also help with protecting the staff and the products being tested. Some
safety equipment that is common only used is:

 Biological Safety Cabinet (Laminar Flow Hood)

 protects you and the materials you are working with

 Chemical Fume Hood

 Centrifuge Safety Canister

 Sharps container & Broken glass box


White Paper: Biosafety and Blood Borne
Pathogen Safety in the Laboratory

 Biohazard waste container

 Personal Protective Equipment

 gloves, lab coats, safety glasses, goggles, face shield, gowns, shoe covers

Facility design and construction are also helpful when it comes to protection. Security
measures and physical separation of lab work areas from areas of public access are
important methods of containment. Some examples could be:

 Swipe card access to research hallways

 Key access to research suites

 Break areas outside of research areas

 Availability of decontamination stations

 Handwashing and eye washing facilities

 Emergency eyewash and shower stations in hall

 Autoclave

 UV lights in Biosafety Cabinets

 Separate ventilation systems

Biosafety levels can help laboratories manage how much containment they need to operate.
BSL1-4 represent conditions under which the agent can be safely handled. Combinations of
lab facilities, lab practices, techniques, safety equipment. Biosafety levels are selected
White Paper: Biosafety and Blood Borne
Pathogen Safety in the Laboratory

based on operations performed and routes of transmission of infectious agents and/or


rDNA.

The single most effective measure to control the transmission of Bloodborne Pathogens is
are to employ universal precautions. Treat all human blood and other potentially infectious
materials like they are infectious for Hepatitis B and HIV, guidelines to reduce the risk of
exposure:

 Frequent hand washing

 Scrubbing with soap and warm water for 20 seconds (sing “Happy Birthday”
twice through)

 Consistent use of PPE

 Observance of Universal Precautions

 Vaccination against Hepatitis B virus

Treat all blood and body fluids as infectious. Use gloves for contact with blood, body fluids,
moist mucous membranes, non-intact skin, secretions and contaminated items. Other
Personal Protective Equipment (PPE) should be worn as needed to suit the task you are
performing. Remove PPE when task completed and moving to a clean area. Disinfect
hands after contact with blood/body fluids/tissue, even if gloves are used. Disinfect
equipment/surfaces per policy. By employing these simple methods and practices you can
be sure to contain any routes of transmission and keep the products and employees safe
and healthy.
White Paper: Biosafety and Blood Borne
Pathogen Safety in the Laboratory

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