Estufa Thermo Scientific - 371
Estufa Thermo Scientific - 371
Estufa Thermo Scientific - 371
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. ▲
Caution All internal adjustments and maintenance must be performed by qualified service personnel. ▲
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
Warning If the incubator is not used in the manner specified in this operating manual, the protection provided
by the equipment design may be impaired. ▲
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and information
on Thermo products will be available at www.thermofisher.com.
✔ Always use the proper protective equipment (clothing, gloves, goggles, etc.)
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-888-213-1790 Toll Free, U.S. and Canada
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take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
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Our Service Support staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo products.
Whatever Thermo products you need or use, we will be happy to discuss your applications. If
you are experiencing technical problems, working together, we will help you locate the problem
and, chances are, correct it yourself...over the telephone without a service call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Warranty Notes
Information You Should Know Before Requesting Warranty Service
• Locate the model and serial numbers. A serial tag is located on the unit itself.
• For equipment service or maintenance, or with technical or special application inquiries, contact Technical
Services at 1-888-213-1790 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local
distributor.
• Chamber Gas Sample Port - Used for sampling chamber CO2 content
using a FYRITE or similar instrument.
Keypad Operation The Model 370 Series incubator has four basic modes which allow
incubator setup: Run, Setpoints, Calibration and System Configuration.
Keypad Operation Scroll for Parameters Arrows: Steps the operator through parameters of
SET, CAL and CONFIG Modes. The right arrow goes to the next
(continued) parameter, the left arrow returns to the previous parameter.
Up Arrow: Increases or toggles the parameter value selected in SET, CAL,
and CONFIG Modes.
Enter: Must press Enter key to save to memory all changed values.
Down Arrow: Decreases or toggles parameter values selected in SET, CAL
and CONFIG Modes.
Silence Key: Press to mute audible alarm. See Section 4 for alarm ringback
times.
Displays Message Center: Displays system status (Mode) at all times. Displays
CLASS 100 or SYSTEM OK during normal operation, or alarm mes-
sages if the system detects an alarm condition (see Section 4, Alarms).
The display message CLASS 100 is a timing mechanism indicating that,
under normal operating conditions with the HEPA filter installed, the air
inside the chamber meets the Class 100 air cleanliness standard for par-
ticulates of 0.5 micron size or larger per cubic foot of air. Further infor-
mation on the Class 100 classification of air quality is available from
Thermo.
Upper and Lower Displays: These 7 segment displays vary, depending on
options present and configuration chosen. The upper display can display
temp or RH, or toggle between them. The bottom display shows CO2
continuously.
Installing the 1. Maintain a minimum three-inch clearance behind the incubator for
electrical and gas hook-ups. In addition, a three-inch ventilation space
Incubator is needed on each side.
2. Locate the unit on a firm level surface capable of supporting the unit’s
weight of 260 lbs. (118kg).
3. Locate the unit away from doors and windows and heating and air
conditioning ducts.
4. Lift the unit only by the sides of the cabinet base. Do not attempt to
lift it by the front and back. This places stress on the outer door
hinges.
Stacking the Warning Install stacked units against a wall or similar structure. ▲
Incubators
Warning With incubators in a stacked configuration, do not leave both
exterior doors open at the same time. ▲
Warning If the units have been in operation, turn them both off and
disconnect the power before beginning any service work. ▲
1. Remove the cover plate securing the door cord from the incubator to
be on top. See Figure 1-4. Disconnect the plug from the connector.
13. Install the cover plate on the other side of the unit.
Preliminary Cleaning 1. Remove vinyl from shelf channels, duct sheets, and air duct, if present.
Installing the Shelves 1. Install the large sheet metal side ducts with the tabs facing into the
center of the chamber with their slots up. There are no right side or
left side ducts, simply turn one of them to fit the opposite side. Tilt
the side ducts as they are placed into the chamber so the tops fit into
the top air duct, then guide them into the vertical position. Figure 1-
10 shows the side duct as it would be oriented for the right side of the
chamber.
Figure 1-10. Shelf Channels and Side Ducts Figure 1-11. Channels and Slots
2. Note that there is no difference in the left and right side shelf channels.
3. Install the shelf channels by placing the channel’s rear slot over the
appropriate rear tab on the side duct. Pull the shelf channel forward
and engage the channel’s front slot into the side duct’s appropriate
forward tab. Refer to Figure 1-11.
4. Figure 1-10 shows one of the channels installed on the right side duct.
Access Port Filter & 1. Locate the opening in the top left corner on the inside of the chamber.
CO2 Sensor Cover Plate Remove the tape covering the opening on the outside of the unit.
2. Locate the stopper with filter in the hardware bag. Lift the metal port
cover and install the assembly in the opening inside the chamber. See
Figure 1-12.
3. Also in the hardware bag is the CO2 sensor cover plate. Install, using
the thumbscrews. Refer to Figure 1-12 for the location.
Installing the Air 1. Remove the filter from the shipping bag.
Sample Filter 2. Install the air sample filter assembly to the black hose barb behind the
top duct.
3. Insert the other end of the filter assembly onto the metal tubing on the
top duct. Refer to Figure 1-13.
Installing the HEPA Caution Be careful when handling the filter. The media can be damaged if
Filter it is mishandled. To avoid damage to the incubator, do not operate the unit
without the HEPA filter in place. ▲
Installing the HEPA 1. Remove the filter from the shipping box.
Filter (continued) 2. Remove the plastic coating from the filter, using caution not to touch
the filter media.
3. Install the filter as shown in Figure 1-13. Refer to Section 5 for HEPA
filter maintenance.
Leveling the Unit Check for level by placing a bubble-style level on one of the shelves. Turn
the hex nut on the leveler counterclockwise to lengthen the leg or
clockwise to shorten it. Level the unit front-to-back and left-to-right.
Connecting the Unit See the serial tag on the side of the unit for electrical specifications or refer
to the electrical schematics at the end of this manual.
to Electrical Power
Caution Serial tag amp rating is based on amperage draw during
sterilization cycle. Normal operating amperage is much less. Ensure that
electrical circuit will handle amp draw of sterilization cycle.
Connect the incubator to a grounded, dedicated circuit. The power cord
connector is the mains disconnect device for the incubator. Position the
incubator so the unit can be easily disconnected. ▲
Plug the provided power cord into the power inlet connector on the back
of the cabinet (Figure 1-14), then into grounded, dedicated, electrical
circuit.
Filling the Humidity Fill the humidity pan with sterile distilled water to within 1/2” of the top.
Place the pan directly in the center of the incubator floor.
Pan
For applications requiring high humidity, the pan should be placed against
the left side wall of the incubator. The side ducts have been modified to
allow the pan to be placed against the wall. Optimum humidity is achieved
by capping the CO2 sample port. This will, however, cause condensation
in the chamber. To enhance RH recovery from door openings, place a
second humidity pan in the right side duct.
Even high purity water can contain bacteria and organic contaminants.
Water should always be sterilized or treated with a decontaminant, safe for
use with stainless steel as well as safe for the product, prior to being
introduced into the humidity pan.
Check the level and change the water frequently to avoid contamination.
Do not allow the water level to fluctuate significantly. “Dry-outs” will have
an adverse effect on the humidity level and CO2 calibration of the T/C
units.
Connecting the CO2 Warning High concentrations of CO2 gas can cause asphyxiation! OSHA
Gas Supply Standards specify that employee exposure to carbon dioxide in any eight-
hour shift of a 40-hour work week shall not exceed the eight-hour time
weighted average of 5000 PPM (0.5% CO2). The short term exposure
limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon dioxide
monitors are recommended for confined areas where concentrations of
carbon dioxide gas can accumulate. ▲
Warning If higher purity CO2 is desired inside the incubator (greater than
99.5% pure), the pressure regulator should be constructed with a stainless
steel diaphragm, along with specifying the purity of the CO2 from the gas
supplier. Follow the manufacturer’s instructions to ensure proper and safe
installation of the pressure regulator on the gas cylinder.
Consult your facility safety officer to ensure that the equipment is installed
in accordance with the codes and regulations that are applicable in your
area. ▲
The CO2 gas supply being connected should be industrial grade 99.5%
pure and should not contain siphon tubes. Install a two-stage pressure
regulator at the cylinder outlet. The high pressure gauge at the tank should
have 0-2000 psig range. The low pressure gauge, at the incubator inlet,
should have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa).
The incubator has serrated fittings on the back of the cabinet to connect
the gas supply. Refer to Figure 1-14. The fitting is labeled CO2 Inlet #1
Tank. Make sure that the connections are secured with clamps. Check all
fittings for leaks.
For units having the CO2 Gas Guard option, refer to Section 6.
Incubator Start-Up With the incubator properly installed and connected to power, the
humidity pan filled, and the unit connected to gas supplies, system
setpoints can be entered. The following setpoints can be entered in Set
mode: Temperature, Overtemperature and CO2. To enter Set mode, press
the Mode key until the Set indicator lights. Press the right and/or left
arrow keys until the proper parameter appears in the message center. See
Chart 1-1 for more detail.
Setting the Operating All Model 370/380 Series incubators have an operating temperature range
Temperature of 10°C to 50°C, depending on ambient temperature. The incubator is
shipped from the factory with a temperature setpoint of 10°C. At this
setting, all heaters are turned off.
2. Press the right arrow until “Temp XX.X” is displayed in the message
center.
3. Press the up/down arrow key until the desired temperature setpoint is
displayed.
5. Press the Mode key until the Run indicator lights for Run mode or
press the right/left arrow keys to go to next/previous parameter.
Setting the Overtemp Caution The independent overtemp system is designed as a safety to
Setpoint protect the incubator only. It is not intended to protect or limit the
maximum temperature of the cell cultures or customer’s equipment inside
the incubator if an overtemp condition occurs. ▲
2. Press the right arrow until Otemp XX.X is displayed in the message
center
3. Press the up or down arrow key until the desired Overtemp setpoint is
displayed
5. Press the Mode key until the Run indicator lights or press the right or
left arrow to go to the next or previous parameter.
Setting the CO2 Setpoint All T/C CO2 cells are calibrated at the factory at 37°C, high humidity, and
10% CO2. Therefore, if a temperature setpoint of 37° C has been entered,
the humidity pan has been filled and the CO2 control is to run between 0-
10% with a T/C CO2 sensor, the CO2 setpoint may be entered
immediately. Otherwise, it is important to allow the unit 12 hours to
stabilize at the temperature setpoint before entering the CO2 setpoint.
All models of the incubator have a CO2 setpoint range of 0.0% to 20.0%.
The incubator is shipped from the factory with a CO2 setpoint of 0.0%.
At this setting, all CO2 control and alarms are turned off. To change the
CO2 setpoint:
2. Press the right arrow until “CO2 XX.X” is displayed in the message
center.
3. Press the up/down arrows until the desired CO2 setpoint is displayed.
5. Press the Mode key until the Run indicator lights to go to Run mode
or press the right/left arrow keys to go to next/previous parameter.
Temperature Before calibration, allow the cabinet temperature to stabilize. Place the
calibrated instrument in the center of the chamber. The instrument
should be in the airflow, not against the shelf.
Thermal Conductivity Models 370 and 371 have a thermal conductivity (T/C) CO2 sensor.
CO2 System Thermal conductivity of the incubator atmosphere is not only effected by
the quantity of CO2 present but also by the air temperature and water
vapor present in the incubator atmosphere. In monitoring the effects of
CO2, air temperature and absolute humidity must be held constant so any
change in thermal conductivity is caused by a change in CO2
concentration.
Start -Up - The CO2 sensor has been calibrated at the factory for 37°C
and elevated humidity. Allow the temperature, humidity, and CO2 levels in
the chamber to stabilize at least 12 hours before checking the CO2
concentration with an independent instrument.
Presently Operating - Make sure the chamber doors are closed. Allow at
least 2 hours after the temperature and CO2 displays reach their setpoints
for chamber atmosphere stabilization.
Thermal Conductivity 1. Make sure the stabilization periods outlined above are followed.
CO2 System (continued) 2. Sample the chamber atmosphere through the sample port with an
independent instrument. Sample the atmosphere at least 3 times to
ensure accuracy of the instrument.
4. Press the right arrow until “CO2 CAL XX.X” is displayed in the
message center.
7. Press the Mode key to return to Run or the right or left arrows to go to
the next/ previous parameter.
Infrared CO2 System Models 380 and 381 have an infrared (IR) CO2 sensor. Infrared CO2
sensors are not effected by chamber atmosphere temperature or humidity.
However, the light detector in the sensor is effected by wide temperature
changes. Therefore, changing temperature setpoints could necessitate a
recalibration of the CO2. Chamber temperature should be allowed to
stabilize before checking CO2 concentrations with an independent
instrument, especially on start-up.
Start-Up- Allow the temperature and the CO2 of the cabinet to stabilize at
least 12 hours before proceeding.
Infrared CO2 System 1. Measure the CO2 concentration in the chamber through the gas sample
port with a Fyrite or other independent instrument. Several readings
(continued) should be taken to ensure accuracy.
3. Press the right arrow until “IR CAL XX.X” appears in the message
center.
Relative Humidity All Model 370/380 Series incubators can be equipped with an optional
direct-readout relative humidity sensor. This is a readout only of the
chamber relative humidity level. It does not provide any control of the
relative humidity in the cabinet.
Start-Up - Allow 12 hours for the relative humidity and temperature in the
chamber to stabilize before proceeding.
3. Press the right arrow key until “RH CAL XX” appears in the message
center.
Relative Humidity If a reliable RH measuring device is not available, the display can be
(continued) calibrated to a typical level;
2. With a full humidity pan and stable temperature, the relative humidity
in the chamber will be 95%.
3. Using Step 3-5 of the relative humidity sensor adjustment, adjust the
display to 95%.
Turn Audible Alarm The audible alarm can be turned on or off. The factory setting is ON.
ON/OFF 1. Press the Mode key until the Config indicator lights.
2. Press the right arrow until AUDIBLE XXX displays in the message
center.
New HEPA Filter When the REPLACE HEPA reminder displays and the visual alarm
flashes, the specified time has elapsed and the HEPA filter should be
replaced. To clear the display and reset the timer after replacing the HEPA
filter with a new one, follow the steps below.
2. Press the right arrow until NEW HEPA displays in the message center.
3. Press Enter to restart the timer and clear the REPLACE HEPA alarm.
Set REPLACE HEPA A HEPA filter replacement timer can be set for a specific amount of time,
from 1 to 12 months of actual unit running time. Time will not accrue
filter reminder when the unit is turned off. The default time is 6 months. When the
allotted time runs out, REPLACE HEPA appears in the display and the
visual alarm flashes. To set the reminder, use the following procedure.
Note After the reminder has been set, check the allotted time remaining by
going to Config Mode, then pressing the right arrow until NEW HEPA
XXX displays. This number is the remaining days before the filter
replacement time specified runs out. For example, if 12 months was chosen
in the REPL HEPA XX message screen, the NEW HEPA number would
be 365 days. ▲
Set Access Code A 3-digit Access Code can be entered to avoid unauthorized personnel
from changing the setpoints, calibration, or configuration. A setting of
000 bypasses the access code. The factory setting is 000.
2. Press the right arrow until ACC CODE XXX displays in the message
center.
Set Low Temp Alarm The low temp alarm limit is the deviation from the temperature setpoint
which causes a low temp alarm. The low temp alarm is variable from 0.5°
Limit (tracking alarm) below setpoint to 5° below setpoint. The factory setting is 1° below
setpoint. A minus sign (-) in the display indicates that the alarm setting is
below the setpoint.
2. Press the right arrow until TEMP LO -X.X displays in the message
center.
Enable Low Temp The low temperature alarm can be programmed to trip the remote alarm
contacts. A setting of ON allows this, an OFF setting blocks the low temp
Alarm to Trip Contacts alarm from tripping the contacts. The factory setting is ON.
5. Press the Mode key to return to Run or the right/left arrow key to go
to next/previous parameter.
Set Low CO2 Alarm The low CO2 alarm limit is the deviation from the CO2 setpoint which
will cause a low CO2 alarm. The setpoint is variable from 0.5% CO2
Limit (tracking alarm) below setpoint to 5.0% CO2 below setpoint. The factory setting is 1.0%
CO2 below setpoint. A minus sign (-) in the display indicates that the
alarm setting is below the setpoint.
2. Press the right arrow until CO2 LO -X.X displays in the message
center.
Set High CO2 Alarm The high CO2 alarm limit is the deviation from the CO2 setpoint which
will cause a high CO2 alarm. The setpoint is variable from 0.5% CO2
Limit (tracking alarm) above setpoint to 5.0% CO2 above setpoint. The factory setting is 1.0%
CO2 above setpoint.
2. Press the right arrow until CO2 HI X.X displays in the message center.
Enable CO2 Alarms to High and Low CO2 alarms can be programmed to trip the remote alarm
Trip Contacts contacts. A setting of ON allows this, a setting of OFF blocks CO2 alarms
from tripping the contacts. The factory setting is ON.
2. Press the right arrow until CO2 RLY XXX displays in the message
center.
Set New Zero Number If a new T/C CO2 sensor is being installed, the two numbers on the
factory installed sticker on the T/C cell must be entered to calibrate CO2
for T/C CO2 Sensors in the unit.
2. Press the right arrow until T/CZ# XXXX displays in the message
center.
3. Press up/down arrow to change the zero number to match the sticker.
Set New Span Number If a new T/C CO2 sensor is being installed, the two numbers on the
for T/C CO2 Sensors factory installed sticker on the T/C cell must be entered to calibrate the
CO2 in the unit.
Note For the technician’s convenience, a label containing the two numbers
on the T/C cell is affixed inside the electronics drawer. ▲
2. Press the right arrow until T/CS# XXXX displays in the message center.
3. Press up/down arrow to change the span number to match the sticker.
Setting a Low RH On units with the RH option installed, a low RH alarm limit may be
entered. The low RH alarm limit is the %RH in the cabinet that causes a
Alarm Limit low RH alarm. The setpoint is variable from setpoint 0 to 90% RH. The
factory setting is 0% RH, which disables the alarm.
Enable RH Alarms to The low RH alarm can be programmed to trip the remote alarm contacts.
Trip Contacts A setting of ON allows this, a setting of OFF blocks the RH alarm from
tripping the contacts. The factory setting is ON.
2. Press the right arrow until RH RLY XXX displays in the message
center.
Enabling Temp/RH to On units equipped with the RH option, the upper seven segment display
on the control panel can be configured to display Temp continuously, RH
be Displayed continuously, or toggle between Temp and RH. If the units does not have
RH, the upper display always displays temperature. If temperature is set to
ON, and the RH is set OFF, temperature displays continuously. If
temperature is set to OFF and RH is set to ON, RH displays
continuously. If both are turned ON, the display toggles between the two.
The factory setting defaults to toggle mode if the RH option is present.
2. Press the right arrow until DISP TMP XXX or DISP RH XXX
displays in the message center.
Select a Primary Tank On units equipped with the Gas Guard option, a primary tank can be
selected. The primary tank will be either Tank 1 or 2. The factory setting
w/ Gas Guard Option is Tank1.
2. Press the right arrow until TNK SEL X displays in the message center.
Disabling the Gas On units equipped with the Gas Guard option, the Gas Guard system may
be turned ON, or OFF if not in use. The factory setting is ON.
Guard System
1. Press the Mode key until the Config indicator lights.
2. Press the right arrow until GAS GRD XX displays in the message
center.
Set an RS485 On units with the RS485 option, direct communication with the Model
1535 alarm system can be established. Each piece of equipment connected
Communications Address to the 1535 must have a unique address. An address of 0-24 can be
entered for the incubator. A setting of 0 is an invalid address that the 1535
ignores. The factory setting for the RS485 address is 0.
When multiple alarm conditions occur, active messages are displayed in the
message center one at a time, updating at 5 second intervals. Pressing
Silence during multiple alarms causes all active alarms to be silenced and to
ring back in 15 minutes.
The TEMP IS LOW alarm is disabled when the Temp set point is 10°C.
The CO2 alarms are disabled when the CO2 set point is 0.0%.
Temp Controller Failure In addition to other safety features designed into Model 370 Series
incubators, a thermostat is also provided to monitor the cabinet’s
TMP CNTR ERR temperature. In the unlikely event of a temperature control failure, the
thermostat will turn off all heaters at a cabinet temperature of 160°C,
±5%. This is intended to be a safety feature to protect the incubator, and is
not intended to protect the cell cultures or the equipment inside the
chamber should a temperature control failure occur. Should such a failure
occur, contact the Technical Services Department or your local distributor.
Sensor Fault Alarms The microprocessor in Model 370 Series incubators continually scans all
available sensors to ensure that they are operating properly. Should an error
be detected, the incubator sounds an alarm and displays the appropriate
message. Contact the Technical Services Department (1-888-213-1790) or
your local distributor.
Your equipment has been thoroughly tested and calibrated before shipment. Regular preventive maintenance is important to
keep your unit functioning properly. The operator should perform routine cleaning and maintenance on a regular basis. For
maximum performance and efficiency, it is recommended the unit be checked and calibrated periodically by a qualified service
technician.
The following is a condensed list of preventive maintenance requirements. See the specified section of the instruction manual for
further details.
We have qualified service technicians, using NIST traceable instruments, available in many areas. For more information on
Preventive Maintenance or Extended Warranties, please contact us at the number listed below.
Cleaning and calibration adjustment intervals are dependent upon use, environmental conditions and accuracy required.
401 Millcreek Road, Box 649 • Marietta, Ohio 45750 USA • 740-373-4763
USA and Canada 888-213-1790 • Telefax: 740-373-4189 • http://www.services.controlenv@thermo.com
Warning If the unit has been in service, turn it off and disconnect the
power cord connector before proceeding with any maintenance. ▲
A HEPA filter replacement kit (see parts list section) should be on-hand
prior to cleaning the incubator interior.
1. Remove the shelves, access port filter assembly, HEPA filter, air sample
filter and tubing, and the left and right duct sheets. Discard the HEPA
filters and the access port filter assembly. See Figure 5-1.
2. Wash the air sample filter tubing, shelves, and ducts with disinfectant,
then rinse with sterile water. Optionally, the shelves and ducts may be
autoclaved.
3. Wash the inner door gasket with disinfectant. This gasket may be
removed to be cleaned, or replaced.
4. Wash the cabinet interior with disinfectant, starting at the top and
working down. Refer to the disinfectant directions for length of time
needed before rinsing. Wash the inner door both inside and out. The
cabinet and door must be rinsed with sterile water until the
disinfectant has been removed. After the cabinet has been rinsed, spray
with 70% alcohol.
5. Install the left and right ducts, inner door gasket, access port filter
assembly, and air sample filter and tubing, spraying each with 70%
alcohol.
Clean Cabinet Clean the incubator exterior with a damp sponge or soft, well-wrung cloth
and mild detergent dissolved in water. Dry with a soft cloth.
Exterior
Clean Glass Doors The chamber glass door and the optional independent inner glass doors
may be cleaned using the same disinfectant as used on the incubator
interior. It is imperative that they be rinsed with sterile distilled water to
remove the disinfectant residue. The doors should then be dried with a soft
cloth.
Some precautions in the cleaning and care of the incubator glass doors:
Moisture leaches alkaline materials (sodium, Na) from the surface of the
glass. Evaporation of the moisture concentrates the alkaline and may
produce a white staining or clouding of the glass surface. Cleaning
chemicals with a PH above 9 and heat (autoclaving) accelerate the
corrosion process. Therefore, it is very important to rinse and dry the glass
doors after cleaning. Autoclaving the glass doors should be avoided.
There is no simple method for repairing corroded glass. In most cases, the
glass must be replaced.
Clean Humidity Pan Clean the humidity pan with soap and water and a general-use laboratory
disinfectant. Rinse with sterile water and spray with 70% alcohol. The pan
may be autoclaved.
6. Rotate the inner door 180° and secure the door hinges
to the unit. Install the two Phillips screws into the
holes between the hinges.
Figure 5-5.
7. Locate the cord cover on the bottom of the outer Knob Orientation
door. See Figure 5-6. Remove
the eight screws securing the
cover, arrange the cord toward
the opposite side and reinstall
the cover.
9. Remove the hinges from the outer door. Carefully pry off the nylon
screw cover from between the hinges. Remove the screw.
10. Install the hinges on the opposite side of the door. Install the screw and
screw cover between the hinges.
11. Install the door handle opposite the hinges. Install screw covers.
12. To secure the outer door to the unit, the Phillips screws on the side of
the unit must be removed and installed on the opposite side.
HEPA Filter Replace the HEPA filter when the REPLACE HEPA reminder is
displayed. The REPLACE HEPA reminder can be set to alarm after a
Maintenance specified time from 1 to 12 months. The reminder default is the factory
recommended setting of 6 months. For details, see Section 3.
Replace Power Fuses To access the only replaceable fuse in the incubator:
1. Turn off the incubator’s power switch and unplug the power cord.
2. Remove the two screws from the top of the control panel. Grasp the
control panel on each side and pull straight outward.
4. Figure 5-8 shows the location of the fuse. See Table 5-1 following for
replacement fuse specifications.
5. Secure the control panel and return the unit to service. If the fuse
blows after restoring power to the incubator, contact the Technical
Services Department.
Table5-1. Fuse Replacement Chart
Fuse Voltage Manufacturer’s P/N Amperage Rating Rupture Speed IEC Letter Code
115V ACC BUSS GMC-1.0A 1.0 Amp Time-Lag T
230VAC ACC BUSS BK-GMC-500mA 0.5 Amp Time-Lag T
Replace Air Sample 1. Connect one end of the air sample filter to the hose barb on the
chamber ceiling.
Filter
2. Connect the other end of the filter to the metal tubing on the HEPA
filter adapter (Figure-5-8).
Electronics Section 1. Turn off the incubator’s power switch and unplug the power cord.
2. Remove the three screws from the back of the cabinet top.
Major Components Refer to Figure 5-9. Some components shown are factory installed options.
Sterilization Cycle
• During the Sterilization Cycle, the incubator updates the temperature to the analog output
board and the 1535, however CO2 will be fixed at setpoint and RH will be fixed at RH
Low Limit plus 1%.
• A HEPA filter replacement kit (see the spare parts list) should be on hand prior to
initiating the Sterilization Cycle.
• Pre-cleaning may be required. In order to avoid odors, stains on the interior, baked-on
material, etc., wipe off all visible signs of spills.
• Odor may occur during the Sterilization Cycle and is considered normal.
• The Steri Cycle is not intended to sterilize other items; instruments, etc., from the lab.
• During the cycle, the unit chamber becomes hot enough to melt samples, instruments,
dishes, etc., left inside the unit. The items listed below also need to be removed.
1) HEPA filter
2) Air sample filter
3) Access port filter assembly
4) Water in the humidity pan
5) Temp/RH recorder probe, if applicable
6) IR sensor, if applicable
Checkpoints
What if? Then
Units are stacked A Sterilization Cycle performed on either unit affects performance on second unit.. Do
not use second unit during Sterilization Cycle. The Sterilization Cycle may be performed
on both units simultaneously, with proper electrical connection.
Unit is not powered off before removing the I/R sensor, if applicable
Damage to the sensor may result
The outer door is open longer than 20 seconds during phases listed above
Cycle is canceled, unit goes to CANCELED COOL PHASE*
The outer door is open during the cool down phase when the temp is 60°C or greater
An outer door alarm occurs
Sterilization Cycle Warning The Sterilization Cycle heats the incubator interior surfaces to
140°C. Contact with any surface inside the outer door during this cycle
may result in burns. ▲
1. Empty the water from the humidity pan and place the pan back into
the incubator. Remove any samples, instruments, dishes, etc. from the
chamber.
2. Press and hold the large green sterilization cycle button on the right
side of the unit (Figure 5-10) for approximately 3 seconds until the
LED lights.
4. Pre Sterilization with T/C CO2 sensor - The display toggles between
“REMOVE HEPAs”, “REMOVE WATER” and “PRESS ENTER”. If
Enter is not pressed within 1 minute, the display returns to “SYSTEM
OK”.
5. Remove the HEPA filter, air sample filter and access port filter
assembly (Figure 5-11). Tubing can remain in the unit during the
sterilization cycle. In addition, remove the temp/RH recorder probe
and IR sensor, if applicable. Discard the filters.
Note To remove the IR sensor, if applicable, first power the unit off. Then
turn the two thumbscrews on the top duct until the cover plate disengages.
Secured on the inside of the plate is a dummy sensor (Figure 5-12). ▲
Grip the IR sensor and carefully pull it downward. Unscrew the cable from
IR sensor (Figure 5-13). Set the sensor aside. See Step 9 for the IR sensor
disinfection procedure. Connect the dummy sensor to the cable. Fit the
installed dummy sensor up into the previous sensor location.
When the power is turned on with the IR sensor removed, the display
toggles between “REMOVE HEPAs”, “REMOVE WATER”, and “PRESS
ENTER”. If “ENTER” is not pressed within 1 minute, the display toggles
between “POWER OFF” and “REPLACE IR”. When the unit is powered
back on with the IR sensor reinstalled, the display returns to “SYSTEM
OK”.
6. After ENTER is pressed, the Heat Phase initiates. The green LED
flashes and the display toggles between “STERILIZING” and “HEAT
PHASE”. During this period, the HEAT light will be on and the
cabinet heats to sterilization temperature.
7. When the air temperature in the unit reaches 140.0°C, the Sterilization
Phase begins and the display changes to “STERILIZING”.
Power the unit off, open the chamber, and remove the IR plate. Pull down
on the dummy, unscrew the cable from the top of the dummy and press
the dummy into the clip on the plate. See Figure 5-12. Screw the cable
onto the top of the IR sensor. The cable is keyed and can only be inserted
one way. Reinstall the sensor by pushing it as far as it will go into the hole.
After the unit is powered back on with the IR sensor reinstalled, the
display toggles between “CYC COMPLETE”, “REPL HEPAs”, and
“PRESS ENTER”.
10. Open the chamber and install a new HEPA filter, air sample filter and
access port filter assembly. Press ENTER.
11. When ENTER is pressed, the LED goes out, the display returns to
“SYSTEM OK”, and the HEPA filter replacement timer is reset.
12. Fill the humidity pan with 3 liters of sterile distilled water within the
recommended purity range (See Section 1).
13. Allow to stabilize for at least 12 hours at the required temperature and
CO2 level. It is recommended that the temperature and CO2
calibrations be verified periodically during the first week of returning
the unit to service.
Caution The high temperature sterilization cycle may cause the T/C CO2
sensor output to change significantly. (This is normal and does not
indicate damage to the sensor.) Therefore it is essential that the CO2
calibration is verified before returning the unit to service. ▲
Note If the Cool Phase is canceled, the display toggles between “CYC
CANCELED” and “COOL PHASE”.
• With T/C CO2 sensor - When the temperature cools down to the
original set operating temperature or 30°C, whichever is higher, the
display toggles between “CYC CANCELED”, “REPL HEPAs”, and
“PRESS ENTER”. The green LED is lighted but no longer flashes.
Remote Alarms A set of relay contacts is provided to monitor alarms through an RJ11
telephone style connector on the back of the cabinet. Refer to Figure 6-5
for the location of the alarm connector. The 12-foot telephone cord (P/N
190388) and RJ11-to-screw terminal conversion box (P/N 190392) are
available through the Technical Services department.
RS485 Interface All incubator models can be purchased with the RS485 communications
(P/N 1900085) option. This option allows the incubator to be directly connected to a
Model 1535 alarm system without the use of a communications module. A
junction box is provided with each RS485 option. Refer to Figure 6-2 for
wiring details. Figure 6-5 shows the location of the RS485 connector on
the back of the incubator cabinet.
Analog Output Boards Warning The electronics section contains hazardous voltages. Only
(P/N 190512, 190543, 190544) qualified personnel should access this area. ▲
The analog output board is an option that allows the incubator to output
analog signals representing the air temperature of the cabinet, CO2
content, and relative humidity, depending upon which systems are in the
incubator. There are three different analog output board options available :
0-1V, 0-5V, or 4-20mA signals. Negative display readings will output 0V.
The outputs do not have isolated grounds. See Table 6-1 for output
specifications of the three boards.
Table 6-1. Analog Output Board Specifications
190512 4-20 mAOutput 190544 0-1VOutput 190543 0-5VOutput
Scaling 4-20 mA Equals Scaling 0-1 V Equals Scaling 0-5V Equals
Temperature 12.5-162.5°C 0.0-150.0°C 0.0-150.0°C
RH 0-100 %RH 0-100 %RH 0-100 %RH
CO2 0.0-100.0 %CO2 0-100.0 %CO2 0-100.0 %CO2
Analog Output Boards For the 0-1V and 0-5V boards, the recording device must supply a load
>/=1000 ohm. For the 4-20mA board, the recorder must supply a load of
(continued) </=100 ohm.
This wire is readily available from other vendors including Alpha P/N
2403 and Deerborn P/N 972203.
1. Turn off the incubator and unplug it from the wall outlet.
2. Remove the screws from the back of the cabinet top to access the
electronics area. Slide the top back and off.
7. Secure with screws removed earlier and return the unit to service.
CO2 Gas Guard Warning CO2 can be lethal in high concentrations. Refer to warnings in
(P/N 1900086) Section 1 of this manual. ▲
Model 370 Series incubators can be equipped with a built-in Gas Guard
system that will operate with a CO2 gas supply. The Gas Guard uses two
pressure switches to continuously monitor the pressures of two
independent CO2 supplies and automatically switches from one supply to
the other when the supply pressure drops below 10 psig (0.690 bar). The
Gas Guard’s design does not facilitate use by multiple incubators.
The CO2 gas supplies must be equipped with two-stage pressure regulators
with gauges. The high pressure gauge at the tank should have a 0-2000
psig range and the low pressure gauge should have a 0-30 psig range. The
gas supply to the incubator must be maintained at 15 psig (1.034 bar), ±5
psig. Gas pressures below 15 psig will cause nuisance alarms to occur on
incubators equipped with the built-in Gas Guard.
Connecting CO2 Gas The CO2 inlets for the Gas Guard are located on the rear of the cabinet.
Using 1/4” ID tubing, connect one of the CO2 supply tanks to the fitting
Supplies
labeled CO2 Inlet #1 Tank. Connect the second CO2 supply tank to the
fitting labeled CO2 Inlet #2 Tank. Install 3/8” hose clamps to secure the
1/4” ID tubing to the fittings on the rear of the drawer. (See Figure 6-5.)
De-activating Gas Guard The built-in Gas Guard is turned ON when shipped from the factory. In
addition, the Tank Sel for the Gas Guard is specified as Tank 1 when
shipped. Refer to Section 3, Configuration, to de-activate the Gas Guard
or change the Tank Sel from #1 to #2. If the Gas Guard system is not
used, the incubator functions normally by supplying CO2 from the supply
tank connected to Inlet #1.
CO2 Gas Guard Operation With the Gas Guard in operation, the incubator uses the gas supplied by
the tank connected to Inlet #1 until the pressure drops below 10 psig
(0.690 bars). At this time, the Gas Guard automatically switches to the
gas supplied through CO2 Inlet #2.
CO2 Gas Guard Operation Audible and visual alarms occur on the control
panel when the gas guard switches from one
(continued)
supply to the other. The audible alarm sounds
until the operator presses the Silence key on
the control panel. The visual alarm in the
Message Center reads Tank 1 Low while the
audible alarm is sounding, but the message is Figure 6-6. Alarm Response
removed when the operator presses the Silence
key. However, the ‘Tank Low’ indicator on the
control panel stays lighted until the condition is
resolved. The unit operates normally.
Caution Both the audible and visual alarms described above do not ring
back after the Silence key is pressed. ▲
If the Gas Guard system does not detect an adequate gas supply at CO2
Inlet #1 or Inlet #2, a visual and audible alarm again occurs on the control
panel. The visual alarm in the Message Center reads Tank 1&2 Low. The
audible alarm continues to ring until the Silence key is pressed. The
audible alarm ring backs every 15 minutes after the alarm is silenced if the
Gas Guard continues to detect that both gas supply pressures are below 10
psig (0.690 bars).
Humidity Readout Model 370/380 Series incubators can be equipped with a humidity sensor
(P/N 1900091) to monitor the relative humidity inside the chamber. The sensor is located
on the chamber ceiling and provides a signal that is displayed in 1%
increments on the control panel. The humidity readout can display
continuously, or toggle with the temperature readout. In addition, a low
alarm limit can be set on the humidity readout to detect when the
humidity pan runs dry. Refer to Section 3, Configuration.
Factors which may affect humidity levels in the chamber are: the water
level in the humidity pan, frequency of door openings, and the fact that
the humidity pan is located on the bottom of the chamber versus on a
shelf.
Temperature
Control . . . . . . . . . .±0.1°C Microprocessor PID Control
Setpoint . . . . . . . . .Digital - Touch pad, 0.1°C
Range . . . . . . . . . . .+5°C above ambient to 50°C
Uniformity . . . . . . .±0.3°C @ +37°C
Tracking alarm . . . .User programmable (low) indicator
Overtemp . . . . . . . .Tracking, user programmable, action, and indicator
Display . . . . . . . . . .Digital, LED, 0.1°C increments
Temperature Safety
Type . . . . . . . . . . . .Extreme temperature safety, action, and indicator
Sensor . . . . . . . . . . .Thermostat, independent of temp control system
Indicator . . . . . . . .Message center, audible and visual alarms
Relative Humidity
Control . . . . . . . . . .Humidity pan - natural vaporization
Humidity w/ pan . .95% RH at 37°C
Display . . . . . . . . . .Optional in 1% increments
Alarm . . . . . . . . . . .Low RH with optional RH monitor
CO2
Control . . . . . . . . . .±0.1% microprocessor PID control
Sensor . . . . . . . . . . .T/C or IR
Readability . . . . . . .0.1%
Range . . . . . . . . . . .0 to 20%
Inlet pressure . . . . . .15 psig (1 bar), ±5 psig (0.3 bar)
Display . . . . . . . . . .Digital LED, 0.1% increments
Shelves
Dimensions . . . . . . .18.5” x 18.5” (47cm x 47cm)
Construction . . . . . .Stainless steel (belt sanded, both sides)
Surface area . . . . . . .2.4 sq. ft. (0.22 sq. m) per shelf
Max. per chamber . .36.0 sq. ft. (3.34 sq. m)
Standard . . . . . . . . .4
Maximum . . . . . . . .15
Construction
Interior volume . . . .6.5 cu. ft. (184 liter)
Interior . . . . . . . . . .Type 304 stainless steel shiny finish
Exterior . . . . . . . . . .18 gauge cold roll steel
Outer door gasket . .Four-sided molded, magnetic Santoprene
Inner door gasket . .Bulb, silicone
Insulation . . . . . . . .Mineral wool
Fittings
Access port . . . . . . .1-1/4 inch (32mm) removable silicone plug
CO2 inlet . . . . . . . .1/4” (6.4mm) barbed
Sample port . . . . . .Front mounted barbed
Electrical
115 Volt models . . .115VAC, 50/60 Hz, 1PH, 9.6 FLA
(Operating range 90-125VAC)
230 Volt models . . .230VAC, 50/60 Hz, 1 PH, 4.4 FLA
(Operating Range 180-250VAC)
Power switch/circuit breaker . . 2 Pole, 12.0 Amp
Accessory outlet . . .Voltage equal to the cabinet input.
75 Watts maximum, 0.5ma leakage current
Alarm contacts . . . .Deviation of temperature, CO2, power,
NO and NC
Dimensions
Exterior . . . . . . . . . .26.1” W x 39.5” H x 25.0” F-B
. . . . . . . . . . . . .(66.3cm x 100.3cm x 63.5cm)
Interior . . . . . . . . . .21.3” W x 26.8” H x 20.0” F-B
. . . . . . . . . . . . .(54.0cm x 68.1cm x 50.8cm)
Weight
Net . . . . . . . . . . . . .260 lbs (118kg)
Net shipping . . . . . .315 lbs (143kg)
Copper Options
Perforated shelf
Customer Installed: P/N 224166 - solid copper
Humidity pan
Customer Installed: P/N 237015 - solid copper
Copper interior components kit
Factory installed: Replace stainless steel duct work, shelves, and
humidity pan with solid copper P/N 1900095
Certifications
Refer to the Declaration of Conformity at the end of this manual
Safety Specifications
Indoor Use Only
Altitude . . . . . . . . . .2,000 meters
Temperature . . . . . .5°C to 40°C
Humidity . . . . . . . .80% RH at or below 31°C, decreasing linearly to
50% RH at 40°C
Mains Supply Fluctuations
Mains supply voltage fluctuations not to exceed ±10% of nominal
voltage
Installation Category II1
Pollution Degree 22
Class of EquipmentI
__________________________________________________________
1 Installation category (overvoltage category) defines the level of transient overvoltage
which the instrument is designed to withstand safely. It depends on the nature of the
electricity supply and its overvoltage protection means. For example, in CAT II which
is the category used for instruments in installations supplied from a supply comparable
to public mains such as hospital and research laboratories and most industrial laborato-
ries, the expected transient overvoltage is 2500V for a 230V supply and 1500V for a
120V supply.
2 Pollution Degree describes the amount of conductive pollution present in the operat-
ing environment. Pollution degree 2 assumes that normally only non-conductive pollu-
tion such as dust occurs with the exception of occasional conductivity caused by con-
densation.
BILL OF MATERIALS
ITEM
NO. PART NO. PART DESCRIPTION QTY
26
25 THIS DOCUMENT CONTAINS PROPRIETARY MODEL/PART NAME: 370 INCUBATOR
19 INFORMATION AND SUCH INFORMATION IS NOT TO
BE DISCLOSED TO OTHERS FOR ANY PURPOSE NOR DWG TITLE: 370 BACK PANEL ASSEMBLY
USED FOR MANUFACTURING PURPOSE WITHOUT
WRITTEN PERMISSION FROM THERMO ELECTRON DWN:DALE CAD: DCB APPD: DATE: 3/26/01 SCALE: 0.375
6 MATERIAL: -
23 9 PAINT: N/A
TOLERANCE UNLESS OTHERWISE SPECIFIED DRAWING NUMBER SIZE
Spare Parts
ELECTRON CORPORATION Section 8
Controlled Environment Equipment ANGLES: DECIMAL: .XX=%%p
Box 649, Marietta, Oh 45750
.xxx=%%p 370-200-5 B
8-5
Spare Parts
Section 8
Steri-Cycle CO2 Incubator
18 BILL OF MATERIALS
ITEM
PART NO. PART DESCRIPTION QTY
NO.
17
1 22049 #6-32 X 3/8 SS PHP SCREW 6
2 22053 #8-32 X 1/2 SS PHP SCREW 8
3 23058 #6 SS EXT TOOTH LOCKWASHER 6
14
4 114011 1-1/4" X 1/4" ID GROMMET 1
5 190294 PLASTIC RECESS 1
10
6 190793 INSERT PANEL 1
9 7 230180 CIRCUIT BREAKER SWITCH 1
11
8 360230 MAGNETIC SWITCH 1
9 610055 #8-32 CLIPNUT 2
5 10 730060 .416 SS WASHER 1
11 840035 3/16" HOSE TO 1/8" MPT ADAPTER 1
12 840065 1/4" HOSE TO 1/8" FPT ADAPTER 1
2
13 180172_MOD PLASTIC BEZEL, MODIFIED 1
6 14 1900312-31-1 CONTROL PANEL FRONT 1
15 1900312-31-3 SAMPLE PORT MOUNT 1
1 3
16 16 1900330-31-1 INSERT SPACER 1
17 360236_NUT SWITCH NUT 1
18 360236_SCSWITCH GREEN PUSHBUTTON SWITCH 1
4
15
7
12
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment
or to the component part beyond the original warranty period. The Technical Services Department must give prior approval for
return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to Thermo postage paid
and replacement parts are shipped FOB destination.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED.
NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo Electron
shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of
products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives.
Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at l-888-213-1790 (USA and Canada) or 1-740-
373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and special applica-
tions. Outside the USA, contact your local distributor for warranty information.
ISO
10-1
10-2
Section 10
Section title
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment
or to the component part beyond the original warranty period. The Technical Services Department must give prior approval for
return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to Thermo postage paid
and replacement parts are shipped FOB destination.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED.
NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be
liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives.
Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at 1-888-213-1790 (USA and Canada) or 1-740-
373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and special application.
Outside the USA, contact your local distributor for warranty information.
ISO
9001
Rev. 2 2/07 REGISTERED
www.thermofisher.com