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    Pipette Notes

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    Abduladzis Manalo
    This document discusses different types of pipettes used in clinical chemistry based on their calibration and use. Pipettes are either calibrated to contain (TC) or deliver (TD) a specific volume. Graduated pipettes have larger tolerances than volumetric pipettes. Volumetric pipettes provide the greatest accuracy when diluting standards. Other pipette types include serologic, Mohr, Ostwald-Folin, and Pasteur pipettes. Proper pipetting techniques include using an aspirator bulb rather than mouth pipetting, measuring at the meniscus for viscous fluids, avoiding contact of the tip with accumulating fluid, and thorough cleaning of glass pipettes.

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    Pipette Notes

    Uploaded by

    Abduladzis Manalo
    50 views2 pages
    This document discusses different types of pipettes used in clinical chemistry based on their calibration and use. Pipettes are either calibrated to contain (TC) or deliver (TD) a specific volume. Graduated pipettes have larger tolerances than volumetric pipettes. Volumetric pipettes provide the greatest accuracy when diluting standards. Other pipette types include serologic, Mohr, Ostwald-Folin, and Pasteur pipettes. Proper pipetting techniques include using an aspirator bulb rather than mouth pipetting, measuring at the meniscus for viscous fluids, avoiding contact of the tip with accumulating fluid, and thorough cleaning of glass pipettes.

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    PIPETTE NOTES

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    pipette notes

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    This document discusses different types of pipettes used in clinical chemistry based on their calibration and use. Pipettes are either calibrated to contain (TC) or deliver (TD) a specific volume. Graduated pipettes have larger tolerances than volumetric pipettes. Volumetric pipettes provide the greatest accuracy when diluting standards. Other pipette types include serologic, Mohr, Ostwald-Folin, and Pasteur pipettes. Proper pipetting techniques include using an aspirator bulb rather than mouth pipetting, measuring at the meniscus for viscous fluids, avoiding contact of the tip with accumulating fluid, and thorough cleaning of glass pipettes.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    50 views2 pages

    Pipette Notes

    Uploaded by

    Abduladzis Manalo
    This document discusses different types of pipettes used in clinical chemistry based on their calibration and use. Pipettes are either calibrated to contain (TC) or deliver (TD) a specific volume. Graduated pipettes have larger tolerances than volumetric pipettes. Volumetric pipettes provide the greatest accuracy when diluting standards. Other pipette types include serologic, Mohr, Ostwald-Folin, and Pasteur pipettes. Proper pipetting techniques include using an aspirator bulb rather than mouth pipetting, measuring at the meniscus for viscous fluids, avoiding contact of the tip with accumulating fluid, and thorough cleaning of glass pipettes.

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    © All Rights Reserved
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    of 2

    COMPILED NOTES IN CLINICAL CHEMISTRY Roderick D.

    Balce, RMT 1

    PIPETTING
    1. Types of Pipette According to Calibration
    a. TC (To contain) Pipette
    ▪ holds a particular volume but does not dispense that exact volume
    ▪ calibrated by introducing the exact weight of mercury required to give the desired volume at a specific
    temperature
    b. TD (To deliver) Pipette
    ▪ dispenses the indicated volume
    ▪ calibrated by weighing the volume of water that will flow from the pipette by gravity
    ▪ rate of delivery must never be hastened by blowing
    ▪ when an etched ring is seen near the mouthpiece, remaining drop at the tip after delivery is blown out to
    receiving vessel
    2. Types of Pipette According to Use
    a. Graduated or Measuring Pipettes
    ▪ deliver an amount of liquid contained between two calibration marks
    ▪ with larger tolerances than volumetric pipettes
    ▪ used when great accuracy is not required or when speed is more important than precision
    o Serologic
    • has calibration marks to the tip
    • the rate of fall of liquid is much faster because of a larger orifice
    • generally a blow-out pipette (has an etched band on the suction piece)
    o Mohr
    • calibrated between two marks; no graduation marks to the tip
    • self-draining but the tip should not be allowed to touch the vessel wall while draining
    o Bacteriologic
    o Ball, Kolmer, and Kahn
    o Micropipettes
    • TC pipettes designed as either Mohr or serologic
    • used when small amount of blood or specimen is needed (<1 ml)

    b. Volumetric Pipette
    ▪ has the greatest degree of accuracy and precision and should be used when diluting standards, calibrators
    or quality control materials
    ▪ TD and self-draining pipette designed to dispense one volume without further subdivisions
    ▪ has a cylindrical bulb midway between the mouthpiece and the tip that decreases surface area/unit volume
    and diminishes error from adherent water film
    c. Ostwald-Folin Pipette
    ▪ used with biologic fluids having a viscosity greater than that of water
    ▪ measures smaller volume (2.0 mL or less)
    ▪ has a large oval bulb near the tip and an etched mark or ring near the mouthpiece (blow out)
    d. Pasteur Pipettes
    ▪ no calibration marks; used to transfer biologic fluids without consideration of a specific volume

    3. Important considerations in manual pipetting


    a. Mouth pipetting is not allowed. Use an aspirator bulb or a pipetting pump.
    b. Use the top of the meniscus when measuring viscous and colored solutions.
    COMPILED NOTES IN CLINICAL CHEMISTRY Roderick D. Balce, RMT 2

    c. The tip of the pipette should not be in contact with the accumulating fluid in the receiving vessel during
    drainage.
    d. Immediately after use, glass pipettes are placed in a cylinder containing water and detergent or a commercial
    cleaning solution (sodium hypochlorite), soaked for 30 min, rinsed with tap water 3 times, followed by 1 rinse
    with deionized or distilled, and dried in a hot oven (<140 °C).
    e. If there is not enough fluid to draw all the way to the final graduation mark, the fluid may be drawn to the
    graduation mark of the volume to be transferred.
    f. An alternative to wiping the side of the pipette after drawing liquid is allowing the outside of the pipette to
    gently touch the inner lip of the original vessel to remove any adherent liquid being careful though to avoid
    touching the tip of the pipette and introducing an air bubble.

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