Cambridge International Examinations

Cambridge International General Certificate of Secondary Education

BIOLOGY 0610/53
Paper 5 Practical Test May/June 2017
CONFIDENTIAL INSTRUCTIONS
Great care should be taken to ensure that any confidential information given does not reach the
candidates either directly or indirectly.
* 2 5 9 2 4 3 9 3 8 8 *

If you have any queries regarding these Confidential Instructions, please contact Cambridge stating the
Centre number, the nature of the query and the syllabus number quoted above.

email info@cie.org.uk
phone +44 1223 553554
fax +44 1223 553558

This document consists of 6 printed pages and 2 blank pages.

DC (KN) 129370/3
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READ THESE INSTRUCTIONS FIRST

These Confidential Instructions give details of the apparatus required by each candidate for each
experiment in this paper. A summary of the questions that will be presented to the candidates is
included, where appropriate, to allow the biology teacher to test the apparatus appropriately. Testing
must be done out of sight of all candidates.

No access to the question paper is permitted in advance of the examination.

Centres are reminded that candidates are expected to follow the instructions on the question paper
and record all their results. They will not be penalised if these results are not what they expect.

The Supervisor should make sure the Supervisor’s Report is fully completed and a copy is enclosed
with each packet of scripts.

It is assumed that the ordinary apparatus of a science laboratory will be available, including a supply of
purified water (distilled or deionised).

If arrangements are made for different sessions for different groups of candidates, care must be taken
to ensure that the different groups of candidates are effectively isolated so that no information passes
between them.

All specimens should carry only the code letters and numbers as indicated and their identity
should not be revealed to the candidates.

Supervisors should ensure that all specimens have the correct identity attached to the specimen and
that these are not removed during the examination.

If a candidate breaks any of the apparatus, or loses any of the material supplied, the matter should be
rectified and a note made in the Supervisor’s Report.

Supervisors are advised to remind candidates that all substances in the examination should be treated
with caution. Pipette fillers and eye protection should be used where necessary.

In accordance with COSHH (Control of Substances Hazardous to Health) Regulations, operative in the
UK, a hazard appraisal of the examination has been carried out.

The following codes are used where relevant:

C corrosive MH moderate hazard

HH health hazard T acutely toxic

F flammable O oxidising

N hazardous to the aquatic environment

Centres are reminded that they are not permitted to open the question paper envelopes before the
examination.

If there are any difficulties with any aspect of setting up this practical examination that the Centre is not
able to resolve, it is essential for Centres to contact Cambridge as soon as possible by email to info@
cie.org.uk, by fax to +44 1223 553558 or by phone to +44 1223 553554.

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Question 1

Each candidate should be provided with:

(i) one beaker containing 25 cm3 of solution P, labelled P

(ii) one beaker containing 25 cm3 of solution Q, labelled Q

(iii) one beaker containing 25 cm3 of solution R, labelled R

(iv) 40 cm3 of freshly prepared iodine solution, in a beaker labelled iodine solution

(v) 10 cm3 of 1% starch solution in a beaker labelled starch solution

(vi) two 10 cm3 syringes (without needles)

(vii) one 1 cm3 syringe (without needle)

(viii) three large test-tubes

(ix) one large test-tube rack

(x) container labelled waste

(xi) beaker or wash bottle containing tap water for washing, labelled water for washing

(xii) white tile or white card

(xiii) eye protection

(xiv) plastic gloves

Preparation of solutions

1 dm3 of solutions P, Q and R will be sufficient for a maximum of 10 candidates.

Solution P

Dissolve 2.0 g of ascorbic acid in 700 cm3 of distilled water. Add distilled water to make up to 1 dm3.

Solution Q

Dissolve 1.0 g of ascorbic acid in 700 cm3 of distilled water. Add distilled water to make up to 1 dm3.

Solution R

Dissolve 0.5 g of ascorbic acid in 700 cm3 of distilled water. Add distilled water to make up to 1 dm3.

1% starch solution

Heat 1.0 g of soluble starch in 90 cm3 of distilled water until the liquid clears. Allow the solution to cool
and add distilled water to make up to 100 cm3.

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iodine solution

A commercially prepared iodine solution such as Lugol’s iodine solution may be used.

Alternatively it can be made as follows:

Wear eye protection, disposable gloves and work in a well-ventilated room.

Put 20 g of potassium iodide (KI) into a beaker and moisten the potassium iodide with a few drops of
distilled water.

Add 10 g of iodine (I2) to the moistened potassium iodide. Add a small volume of distilled water and
stir. When no more iodine appears to dissolve add more distilled water and stir. Keep repeating this
procedure until all the iodine has dissolved. Do not exceed the total final volume of 1 dm3.

If necessary add more distilled water to make up to the total final volume of 1 dm3.

The Supervisor (not the Invigilator) is expected to carry out the practical aspects of Question 1 and
record their results in the space provided in the Supervisor’s Report. This must be done during the
examination, using the same apparatus and reagents as the candidates but out of sight of candidates.

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BLANK PAGE

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BLANK PAGE

Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every reasonable
effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the publisher will
be pleased to make amends at the earliest possible opportunity.

To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge International
Examinations Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download at www.cie.org.uk after
the live examination series.

Cambridge International Examinations is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge Local
Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge.

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0610/53

This form should be completed and sent to Cambridge with the scripts.

SUPERVISOR’S REPORT

The Supervisor or Teacher responsible for the subject should provide the following information.

1 Was any difficulty experienced in providing the necessary materials? If so, give brief details.

..........................................................................................................................................................

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2 Give details of any difficulties experienced by particular candidates, giving names and candidate
numbers. Reference should be made to:

(a) difficulties with specimens or materials;

...................................................................................................................................................

(b) accidents to apparatus or materials;

...................................................................................................................................................

(c) assistance provided in the case of colour blindness;

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(d) any other information that is likely to assist the Examiner, especially if this cannot be
discovered from the scripts.

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Other cases of individual hardship, e.g. illness or disability, should be reported direct to Cambridge
on the normal Special Consideration Form as detailed in the Handbook for Centres.

3 During the examination, the Supervisor should, out of sight of the candidates, carry out the
practical aspects of Question 1 using the same apparatus and reagents as the candidates.
Results should be recorded in the space on page 8 (not on a spare question paper). Attach extra
sheets if necessary.

The Invigilator should not carry out Question 1.

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Question 1 results:

4 A plan of work benches, giving details of the candidate numbers of the places occupied by the
candidates for each session, must be enclosed with the scripts.

Declaration (to be signed by the Supervisor)

The preparation of this practical has been carried out so as to maintain fully the security of the
examination.

Signed ...................................................................................

Name (IN BLOCK CAPITALS) ...................................................................................

Centre number ...................................................................................

Centre name .....................................................................................................................................

If scripts are required to be dispatched in more than one packet, it is essential that a copy of the
Supervisor’s Report and the appropriate seating plan(s) are inside each packet.

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