Contents

Hardware/Operating System Configuration................................... 1

Ⅰ. Software Installation Guide........................................................ 2

Ⅱ. User Guide .................................................................................. 5

i. Using the Function Buttons ................................................... 5

ii. Image Requirements.............................................................. 6

iii. Capture Samples................................................................... 7

iv. Capturing Images .................................................................. 8

v. Setting ..................................................................................... 9

vi. Combining Images .............................................................. 10

Note:
The intention of this guide is to provide quick instructions to some of
the key functions of Motic Images Multi-Focus. If more details are
needed or what you are looking for is not contained in this guide,
please consult the documents that come with your software or visit
our website http://www.motic.com.
 Motic Images Multi-Focus
Motic Images Multi-Focus is multi-focal-plane image combination and
manipulation software.

When using a Motic Images Microscope to observe an object with an unsmooth

surface, the result may be a blurry or partially focused image due to the different
focal planes used for observation. In cases such as this, capture the image from
different focal planes and then, using the Multi-Focus software, combine the
captured images to create one sharp image.

Hardware/Operating System Configuration

1. Minimum hardware configuration：CPU PentiumⅡ200；RAM 64M；Hard

Disk Space 400M；Mouse；CD-ROM Drive.
2. Recommended hardware configuration：CPU PentiumⅣ1500；RAM 256M；
Hard Disk Space 1024M；AGP Device with 32M Display Memory；Mouse；
CD-ROM Drive.
3. Operating System： Windows 98se；Windows ME；Windows NT4.0；
Windows 2000；Windows XP.

Note: A large volume of memory is required when assembling images with high
resolution.

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Ⅰ. Software Installation Guide

Please read this guide carefully before you install the software and follow the
instructions outlined below.

1. Insert the Motic Images Multi-

Focus CD into the CD-ROM drive;
and the Setup Wizard will run
automatically.

2. Select either Chinese (Simplified) or

English as the installation language.
The Installation Wizard will start to
run and display the installation
progress.

3. Prepare for installation as the installer

prompts and click “Next”.

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4. To install Motic Images Multi-
Focus, you must accept all the terms
of Software License Agreement. If you
agree, please click “Yes”; if you do not
agree, please click “No” to quit the
installation.

5. Fill in the user information. Please

enter the User Name and Company
Name in the corresponding bars, and
click “Next”.

6. Select from “Typical”, “Compact” or

“Custom”. Click “Back” to review or
change the settings you have
configured. Click “Next” to continue
with the installation and copy the
program files. The system will then
install the software dog driver.

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7. Select “User-defined” and the
destination path for the installation.
The program will be installed at
C:\ProgramFiles\Motic\MoticImages
Multi-Focus1.0 by default. Click
“Next” to accept the default
destination path or click “Browse” to
change the destination path for the
installation.

8. Copying of program files begins. (If

you wish, you can choose to install
the Adobe reader and DirectX9
before the copying of the program
files starts).

9. Click “Finish” when all the program

files have been copied. It is highly
recommended that you restart the
computer to ensure the software runs
properly.

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Ⅱ. User Guide
Instructions on how to use Motic Images Multi-Focus

i. Using the Function Buttons

1. The “Assembly Immediately” button. Used to

assemble the captured images. For more, see
Combining Images below.

2. The “Open” button. Used to import images.

3. The “Save” button. Used to save the combined

image.

4. The “Album” button. Used to display the album. For

more, see Using the Album below.

5. The “Capture” button. Used to capture images with

the imaging device connected to the user’s system.

6. The “Setting” button. Used to set the system

properties.

7. The “About Motic Images Multi-Focus 1.0” button.

Provides information on the current program version
and other information.

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Using the Album

Click to open the Album.

Left-click on the desired image and

drag it to a blank box to load the
image.

Select “Select All” from the

right-click shortcut menu. Users are
then able to left-click on the images
to drag and load all the selected
images.

ii. Image Requirements

1. A strong 3-dimensional effect and clear arrangement is preferable

for region to be captured.
2. When capturing images, adjust the focus knob until the object
becomes blurry and then gradually adjust the focus knob in the
opposite direction until the first sharp object is obtained. From this
point, begin to capture images until the final focal plane is reached.
3. The Z axis will move from far to near the object until the object
becomes blurry again. While capturing, be sure to adjust the Z axis
in the same direction.

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Note：
a) When the adjustment is made from a sharp region to another sharp region,
the step distance should not be too large. The first sharp region should
gradually become blurry while the next sharp region gradually becomes
focused.
b) When capturing images, if the color or brightness is unacceptable, apply
the White Balance or Auto Expose functions from the image capture tools
to adjust the image.
In order to better capture and combine images, please refer to Capture
samples below.

iii. Capture Samples

+ =

Image 1: Focus Location Image 2: Focus Location

& Sharp Region & Sharp Region

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When the adjustment is made from a sharp region to another sharp region, the
step distance should not be too large. It is best to superimpose the edges of the
sharp regions in order to avoid leaving any blurry regions in between. The
combined image then contains only the sharpest regions of the two images:

iv. Capturing Images

Click the Capture button to activate the Motic Live Imaging module to

capture images. (For detailed operation instruction, please consult the Help that
comes with this module.)The image captured will be saved in the folder set in
the Setting tab, and displayed in the album window on the left of the interface.

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v. Setting

Click the Setting button to display the Setting dialog box:

Images Name Prefix: Here, input the prefix of an image name.

Save To Folder：Here, set the default folder where captured images are to be
saved.
Language Settings：From the drop down list, select the desired language
(Chinese or English). To select another language, a corresponding language
database must be built.

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vi. Combining Images

1. Run Motic Images Multi-

Focus and the interface shown on
the left appears:

2. Click to open the Album.

3. Select all the images to be

combined, and drag them into the
image window on the left.

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4. Select a thumbnail image from the
left Image Preview Window to be
displayed in the main image
window.

5. Click and drag on the image in the

main window and it can be moved
within the main window.

6. Click and the images in the

left Image Preview Window will be

combined.

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 7. Click to save the combined

image.

You have now successfully combined the images and saved the
combined and fully focused image.

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 目 录

硬件配置/操作系统要求 ................................................................... 1

一、软件安装指导 ........................................................................... 2

二、软件使用指导 ........................................................................... 5

（一）、功能按键的使用 ........................................................... 5

（二）、进行合并处理的图片拍照要求 ...................................... 6

（三）、拍照实例 ...................................................................... 7

（四）、图像采集 ...................................................................... 8

（五）、软件设置 ...................................................................... 8

（六）、如何进行合并 ............................................................... 9

注意：
本向导的目的是提供 Motic Images Multi-Focus 软件主要功能的快速指导说
明。如果您需要更详细的说明，或者您需要解答的问题在本向导的涵盖内容之
外，请参考软件的联机帮助或者访问我们的网站 http://www.motic.com。
 Motic Images Multi-Focus
Motic Images Multi-Focus 是一个多焦面图片合并处理工具。
当使用显微镜观察表面不平整的物体，显微镜得到的图片由于观察的焦点不
同 引 起图 片其他部 分模 糊。此 时 先分 别拍摄 物体的各个焦点部分，再通过
Multi-Focus，可以将一组图片合并成一张清晰的图片。
使用 Motic Images Multi-Focus 软件，可以解决拍摄显微图像的诸多难题，
应用广泛，最大限度地扩展数码显微镜的功能。

硬件配置/操作系统要求

1、 硬件最低配置：CPU PentiumⅡ200；内存 64M；硬盘空间 400M；

鼠标；光驱。
2、 硬件推荐配置：CPU PentiumⅣ1500；内存 256M；硬盘空间 1024M；AGP
显示卡 32M 显存；鼠标；光驱。
3、 操作系统要求： Windows 98se；Windows ME；Windows NT4.0；Windows
2000；Windows XP

注意：本软件在进行高分辨率、高精度的图像合并时需要大量内存。

1
一、软件安装指导

请在软件安装前认真阅读安装指导，并在安装过程中严格按照下述步骤执行。

1、 根据需要选择相应的安装语言，中文
（简体）或英文。

2、 将 Motic Images Multi-Focus 1.0 安

装光盘放入您的光驱中，计算机将自动执
行 Motic Images Multi-Focus 的安装程序,
安装程序开始自动进行安装向导，显示安
装向导的安装进程。

3、 请按照安装程序的提示做好准备，然
后单击“下一步”按钮。

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4、 您必须接受《软件使用许可协议》的
各 项 条 款 才 能 够 安 装 Motic Images
Multi-Focus 软件。如果您同意该协议，请
单击“是”按钮继续软件安装；如果您不
同意，请单击“否”按钮，这将终止软件
安装。

5、 填写用户信息。请在安装向导的提示
下如实地填写“用户名”栏和“公司名称”
栏，然后单击“下一步”继续。

6、 用户可从“典型”、
“压缩”、
“自定义”
三种安装类型中选择一种合适的安装方
式。单击“上一步”按钮则回到上一面板
可更改刚才进行的安装设定。如果安装设
置已经确定，请单击“下一步”按钮，开
始安装软件加密狗。之后开始进行程序文
件复制。

3
7、 选择“自定义”，选择安装路径。默认
安装路径是 C:\ProgramFiles \Motic\
Motic Images Multi-Focus 1.0，单击“下
一步”按钮接受默认值，单击“浏览”按
钮更改安装路径。

8、 开始复制文件到您的计算机上。
（在此
步骤之前，您可以根据需要选择是否安装
Adobe 阅读器和 DirectX9）。

9、 文件拷贝完毕，单击“完成”按钮完
成软件安装，为了软件正常运行，您最好
重启计算机。

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二、软件使用指导

下面对如何使用 Motic Images Multi-Focus 进行说明：

（一）、功能按键的使用

1、 立即拼接图片按钮，下文将具体阐述；

2、 打开按钮，导入图像；

3、 存储图像按钮，保存拼接完成的图片；

4、 图片相册按钮，下文将具体阐述；

5、 捕捉器按钮，捕捉图像；

6、 设置按钮，设置系统属性；

7、 关于按钮，包括本软件的版本等信息。

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使用图片相册

单击 打开相册，在相册中选择图片，

按住鼠标左键，拖动图片到空白框中，即
完成载入图片的动作。通过相册，可灵活
的选择希望拼接的部分。

点击鼠标右键，在右键弹出菜单中选择
“全选”，可以选择全部图片，按住鼠标
左键，拖动所有图片到空白框中，即完成
载入图片的动作。

（二）、进行合并处理的图片拍照要求

1、 选择拍照的部位应立体感强，层次分明；
2、 拍照时应先将焦距调至最远，目标模糊，缓慢调整焦距直到目标出现第一个
清晰点，以这个清晰点为基础，开始拍摄图片；
3、 焦距由远至近，直到目标再一次模糊，在拍摄图片的过程中，焦距变动的方
向应保持一致。

注意：
1、 在由一个清晰的区域到另一个清晰区域的调节过程中，每次调节的幅度不要
过大，应该是原来清晰的区域逐渐模糊，而下一个清晰点区域逐渐凸显出来。
2、 在拍摄图片时，若颜色、亮度无法满足要求时，可使用拍图工具中的白平衡、
自动曝光功能来调整图片。

6
为了更好地进行图片拍照、合并操作，请参阅拍照实例：

（三）、拍照实例

+ =

图片一的焦距位置及清晰区域： 图片二的焦距位置及清晰区域：

拍图时由一个清晰的区域到另一个清晰区域的调节过程中，每次调节的幅度不要
过大，最好两张图中清晰区域的边缘重合，以避免留下模糊区域。
合并后的图片包含两张图片的清晰区域：

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（四）、图像采集

点击工具栏中的采集按钮 将启动 Motic 动态成像模块（有关此模块

的使用说明请参照该模块自带的帮助文档）。所采集的图像将保存在“设置”分
页设定的保存文件夹中，并在软件左侧的相册窗口中显示。

（五）、软件设置

点击设置按钮，弹出设置窗口：

图像文件名前缀：输入图像文件名的前缀。
保存文件夹：设置采集图片的默认保存路径。
语言选择：选择需要的软件使用语言。
目前系统支持中文和英文两种语言互换，需要选择其他语言，必须要有相应语言
的数据库的支持。

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（六）、如何进行合并

1、 运行 Motic Images Multi-Focus 软件

界面。

2、 单击相册按钮 ，打开相册。

3、 选择需要合并的图片，将其拖入左边
图片窗口。

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4、 在界面左边的图像预览窗口中选择图
片缩略图，可在图像主窗口中看到所选择
图片。

5、 单击主窗口中的图像，可以在主窗口
中移动图像。

6、 点击 ，左边图像预览窗口中的图

片将进行合并。

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 7、 点击 ，将合并后的图片保存。

完成多焦面图像的合并及保存操作。

11
 Microscopes

Motic China Group Co., Ltd.

© 2000-2007 Motic China Group Co., Ltd.版权所有。Motic是Motic China Group Co., Ltd.的注册商标。
Microsoft,Windows以及Windows徽标是Microsoft Corporation的注册商标。
所有其他产品及品牌均为其持有者的商标或注册商标。

© 2000-2007 Motic China Group Co., Ltd. All rights reserved.

Motic is a registered trademark and service mark of Motic China Group Co., Ltd.
Microsoft, Windows and the Windows logo are registered trademarks of Microsoft Corporation.
All other trademarks are the property of their respective owners.

设计更改：因为技术进步，生产商有权在设计上作出革新，不再另行通知。

Design Change: The manufacturer reserves the right to make changes in instrument design in accordance with scientific and
mechanical progress, without notice and without obligation.

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