Ministry of Health &Population Issue No.

:1
Minia Conjoint Laboratory Issue Date:
Title/Number: Issued By:
Personal Protective Equipment SOP Page No.: of

Purpose:
The purpose of this SOP is to establish procedures for proper selection,use
and maintenance of personal protective equipment (PPE).
This SOP describes:
 When PPE is necessary?
 What PPE is necessary?
 How to properly put on (Donning), take off (Doffing), adjust and wear
the PPE?
 The limitations of the PPE?

Scope:
This SOP applies to all laboratory staff who use PPE within Minia
Conjoint Laboratory and is used when determined necessary by risk
assessment to provide personnel protection from potentially infectious
and hazardous materials.

Responsibilities:
1) Laboratory safety responsible ensures that:
a. This SOP is established and implemented effectively
b. PPE is properly selected, stored and used
c. Staff are trained on this procedure and competent prior to PPE use
2) Laboratory worker who use PPE:
a. Follow the procedures outlined in this SOP
b. Properly wear PPE,
c. Attend training sessions on PPE,
d. Care for, clean and maintain PPE, and
e. Inform a supervisor of the need to repair or replace PPE.
3)Laboratory Manager ensures that:
a. PPE is ordered in appropriate styles/sizes.
b. PPE supplies are adequately maintained including replacing worn or
damaged PPE.
c. Periodically reviewing, updating and evaluating the effectiveness of the
PPE SOP.
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 Ministry of Health &Population IssueNo.:1
Minia Conjoint Laboratory Issue Date:
Title/Number: Issued By:
Personal Protective Equipment SOP Page No.: of
Materials:
a) Hand and Arm Protection
-Gloves
-Fluid resistant (latex, nitrile)
- Temperature resistant (high heat)
b) Eye and Face Protection
- Safety goggles
- Face shield
c)Respiratory Protection:
-Surgical masks.
d)Body Protection
- Laboratory coat
Equipment:
None
Records and Forms:
None

Procedures:
A. PPE Requirement/Hazard Assessment
Personal protective equipment is not a substitute for effective
engineering, administrative controls, safe work practices, or other
control measures and should be utilized as a last resort when other
controls are not sufficient to create a safe work area.
General Instructions:
PPE must be used, maintained and stored in accordance with the
manufacturer’s instructions.
PPE must be visually inspected before each use and any damaged
PPE must not be used.
PPE worn within the laboratory should not be worn outside the
Laboratory or other places accessible to the public.
Laboratory Worker should take off any accessories before using
PPE.
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 Ministry of Health &Population IssueNo.:1
Minia Conjoint Laboratory Issue Date:
Title/Number: Issued By:
Personal Protective Equipment SOP Page No.: of

PPE should be placed in an appropriately designated area or

container for storage, washing,decontamination or disposal..
Change PPE as soon as feasible whenever it is compromised, soiled
or torn.
Wear appropriate sizes and keep an adequate supply of PPE
available in the laboratory.
Wash hands whenever PPE is removed.
Do not touch door handles, telephones, computers or other clean
surfaces oritems with gloved hands.
Wear closed-toe shoes and long pants to guard against skin
contamination or chemical exposure.
Do not wear sandals or shorts in the laboratory
Gloves are not 100% leak proof; change gloves periodically and
when soiled and always hands after removing gloves or other PPE.
Gloves will not prevent needle sticks or other puncture injuries.

Donning Gloves:
Wash your hand .
Dry your hand with paper tissue then dispose the tissue.
Check gloves for visible tears before use.
Wear the suitable gloves - Extend to cover wrist of isolation gown
Avoid touching or adjusting another PPE and keep gloved hands
away from face.
If gloves are teared or wetted change it at once.

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 Ministry of Health &Population IssueNo.:1
Minia Conjoint Laboratory IssueDate:
Title/Number: IssuedBy:
Personal Protective Equipment SOP PageNo.: of

Doffing Gloves(Beak Method):

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 Ministry of Health &Population IssueNo.:1
Minia Conjoint Laboratory IssueDate:
Title/Number: IssuedBy:
Personal Protective Equipment SOP PageNo.: of

Chemical Latex/Rubb Butyl Nitrile

er
Aceticacid VG VG VG
Acetone VG VG P
Ammoniumhydroxide VG VG VG
Butylalcohol VG VG VG
Chloroform P P F
Citricacid(10%) VG VG VG
Cyclohexanol F G VG
Ethylalcohol VG VG VG
Ethylether G VG G
Formaldehyde VG VG VG
Glycerin VG VG VG
Hexane P P G
Hydrochloricacid G G G
Hydrogenperoxide(30%) G G G
Lacticacid(85%) VG VG VG
Methylalcohol VG VG VG
Oxalicacid VG VG VG
 Table 1 from the U.S. Department of Energy (Occupational Safety and
Health Technical Reference Manual) rates various gloves protective
qualities against specific chemicals.
 This table will help to select the most appropriate gloves to protect
your Staff. The rating abbreviations are as follows: VG: Very Good; G:
Good; F: Fair; P: Poor (not recommended).

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 Ministry of Health &Population IssueNo.:1
Minia Conjoint Laboratory Issue Date:
Title/Number: Issued By:
Personal Protective Equipment SOP Page No.: of

Face and Eye Protection :

Laboratory worker must use appropriate eye or face protection when exposed to eye
or face hazards:

 Chemical and Biological hazards.

 Dust or mist
 Splashes of liquids
 Extreme Heat and Cold
 Impact or Explosion

Types of Eye and Face Protection:

Safety Goggles

 Safety goggles are tight-fitting eye protection that completely cover the eyes,
eye sockets and the facial area around the eyes and provide protection from
impact, dust,, mists, and splashes.
 Safety goggles can be worn over prescription lenses.
 Safety goggles are available with perforated, port-vented, or non-vented
frames.Safety goggles should be worn when working with liquids that may
splash or if vapor or airborne particulate protection is required.

Face Shields

 Face shields provide general protection to the entire face for a variety of
hazards, such as chemical splash and extreme heat.
 Impact rated face shields can be worn or face shields must be worn over
primary eye protection ( goggles) when there is a potential exposure to flying
fragments or objects, hot sparks from furnace operations, potentialand
extreme temperatures.

Donning goggles and face shields:

 Place over face and eyes and adjust to fit

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 Ministry of Health &Population IssueNo.:1
Minia Conjoint Laboratory Issue Date:
Title/Number: Issued By:
Personal Protective Equipment SOP Page No.: of
Doffing goggles and face shields:
 Outside of goggles or face shield is contaminated!
 To remove, handle by head band or ear pieces
 Place in designated receptacle for reprocessing or in waste
container

Respiratory Protection:
Surgical Mask Donning
Perform hand hygiene
Carefully grab the mask holding the ear loops.
Without touching the outside of the mask, carefully place the mask
on your face.
Remove gloves if used and perform hand hygiene.
Surgical Mask Doffing (Removal)
Perform hand hygiene.
Remove mask by holding the ear loops. The front is contaminated
so remove it slowly and carefully.
Perform hand hygiene.
Body Protection:
Laboratory Coat
Analyst must wear a laboratory coat when inside the lab, and especially when
handling hazardous materials. Lab coats protect clothing and prevent contact
with hazardous materials. Laboratory coats should:
Never be worn(remove) outside of the active laboratory area except
when transporting materials through a hallway to another designated
lab area 7
 Ministry of Health &Population IssueNo.:1
Minia Conjoint Laboratory Issue Date:
Title/Number: Issued By:
Personal Protective Equipment SOP Page No.: of

Fit properly
Be fastened with snaps at all times while in the lab (snaps are
preferred to buttons as they allow for quick removal in the event of
an emergency)
Be cleaned regularly and separately from all other clothing
Fully cover the arms to the wrist – short sleeve lab coats are not
permitted in wet labs
Be tucked into the glove or secured at the wrist to prevent exposing
the skin between the lab coat at the glove as well as to prevent the
sleeve from catching on equipment or coming in contact with other
hazards in the working area

Don(Wear ) PPE in the following order:

1. Lab coat
2. Respiratory protection(surgical mask)(if needed)
3. Face/eye protection(goggles, face mask)—adjust to fit
4. Gloves—extend to cover wrists of lab coat
Doff PPE in the following order:
1. Gloves
 Avoid touching the outside of the gloves with bare hands
 Immediately dispose as biohazardous waste
2. Face/eye protection(goggles, face shield)
3. Lab coat
 Unfastenties/buttons/snaps
 Remove by touching the inside of the lab coat
 Turn inside out
 Fold or bundle and place in designated receptacle for decontamination
or dispose as biohazardous waste
4. Respiratory protection(surgical mask)
 Grasp bottom then top ties or elastics and remove
 Placeindesignatedreceptaclefordecontaminationordisposeas
biohazardous waste
5. Wash hands with soap and water per the Hand Hygiene . 8
 Ministry of Health &Population IssueNo.:1
Minia Conjoint Laboratory Issue Date:
Title/Number: Issued By:
Personal Protective Equipment SOP Page No.: of

References:
- WHO Biosafety Manual.
- OSHA standards & regulations.
- U.S. Department of Energy (Occupational Safety and Health Technical
Reference Manual

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