S3/Bio/AE/P.

Wong Shiu Chi Secondary School

2021/2022 Annual Examination
IS III (Biology)
Class: 3 A / B / C / D Date : 27-6-2022 (Mon.)AM2
Class No: ______ Time allowed : 1 hour
No. of pages : 12 (8+4)
Full marks : 100 marks (3 marks will be awarded to effective communication)
Instructions: (1) Answer ALL questions.
(2) Write ALL your answers on the answer sheets provided only.
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Section A : Fill in the blanks. (30 marks)
1. In the production of biofuels, sugars are fermented by enzymes to produce _ (a)__, which can
be used as a fuel for vehicles.
2. In clothing industry, enzymes are used to break down __(b)__ fibres of jeans so that the jeans
become less stiff.
3. Each enzyme has an _ (c)__ pH at which the rate of reaction is the highest.
4. Phagocytosis means ‘cell eating’. The cell membrane extends out to form foot-like extensions
called (d) .
5. When there is a difference in the concentration of particles between two regions, (e) __
exists.
6. In concentrated sucrose solution, the coloured cytoplasm of the epidermal cells of red onion
shrink and the cells become _(f)__ .
7. The _ (g)__ the distance between two regions of different concentrations, the higher is the
rate of diffusion.
8. _(h)__ span the entire phospholipid bilayer and form pores through water and water-soluble
molecules can pass the membrane.
9. Diagrammatically, a phospholipid molecules can be represented a hydrophilic ‘head’ with two
_ (i)__ ‘tails’
10. For prokaryotic cells like bacterial cell, they have some extensions called (j) for movement.
11. Adipose tissues are found around the internal organs and act as a (k) for protection.
12. Each amino acid molecule has a central carbon atom, an amino group (-NH2), a __(l) group
(-COOH) and a specific side chain.
13. A _ (m) _ molecule is formed by the condensation of one glycerol molecule and three fatty
acids molecules.
14. The heart, blood and blood vessels form the __(n)__ and removing substances to and from
cells.
15. A leaf is an organ composed of epidermal tissue, mesophyll tissue and _(o)__ tissue.
 S3/Bio/AE/P.2

Section B : Multiple Choices (15 marks)

1. The graph below shows the enzyme activity of two different enzymes P and Q over a range
of pH values.

enzyme P
enzyme Q
Enzyme activity

0 2 4 6 8 10 12 14
pH

Which of the following is a correct interpretation of the graph?

A. Enzyme P is less active than enzyme Q.
B. Enzyme Q works best in acidic medium.
C. Enzyme Q is more active than enzyme P in alkaline medium.
D. Enzyme P is found in the human stomach.

2. Which of the following statements about enzymes is correct?

A. A denatured enzyme can resume its activity under optimum conditions.
B. The optimum temperature of most enzymes is 37 oC.
C. The activation energy of an enzyme-catalysed reaction is lower than that of the
uncatalysed reaction.
D. Enzymes are made up of carbohydrates and proteins.

3. A liver extract is added to hydrogen peroxide solution. The gas produced from the mixture
is tested with a glowing splint. What is the result?
A. The glowing splint goes out.
B. A ‘pop’ sound is heard.
C. The glowing splint relights.
D. There is no change in the glowing splint.

4. Which of the following is/are the advantage(s) of using an electron microscope over a light
microscope to observe single celled organisms?
(1) Both living and dead organisms can be observed with an electron microscope.
(2) The structure of the organisms can be shown in greater detail and resolution.
(3) Preparation of specimen for observation with an electron microscope is easier than
that with a light microscope.
A.(1) only B. (2) only
C. (1) and (2) only D. (2) and (3) only
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5. The graph below shows the energy levels of the reactants and product of a chemical
reaction in the absence and presence of its enzymes.

(i)
(iv)
Energy level

reactants (ii)

(iii)
product

Progress of reaction

Which arrow represents the decrease in activation energy between the uncatalysed and
catalysed reactions?
A. (i) B. (ii)
C. (iii) D. (iv)

6. The following shows the different levels of organisation in organisms:

(1) cells (2) organs (3) systems
(4) tissues (5) organelles

Which of the following shows the correct order of the levels from simple to complex in
organisms?
A.(1), (4), (2), (3), (5) B. (3), (2), (4), (1), (5)
C. (5), (1), (4), (2), (3) D. (5), (2), (1), (4), (3)

7. What is the main function of methylene blue solution in the preparation of a temporary
mount of ox corneal cells?
A. to preserve the cells permanently
B. to preserve the colours of the cells
C. to kill the cells
D. to make some transparent structures easier to be observed

8. Which of the following statements about the modern cell theory is/are correct?
(1) Cells are specialised for different functions.
(2) All organisms are made up of one or more cells.
(3) The cell is the basic unit of life.
A. (1) only B. (1) and (3) only
C. (2) and (3) only D. (1), (2) and (3)

9. Which of the following structures is present in all living human cells?

A. cell wall B. nucleus
C. large vacuole D. cell membrane
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10. In an experiment, a student accidentally added additional 20 mL of substrate into the

reactants containing enzymes. He found that the rate of reaction has changed. What is the
possible change and explanation for the change?
A. The rate of reaction decreased because the enzyme-to-substrate ratio decreased.
B. The rate of reaction decreased because the reactants were diluted by the extra substrate
solution.
C. The rate of reaction increased because the substrates collide more frequently with the
enzymes.
D. The rate of reaction increased because there are more active sites to bind with more
substrates.

11. A student proposed that eating apples can speed up the recovery from a flu. What prediction
could be made based on this?
A. Apple juice can stop the multiplication of viruses in our body.
B. Eating apples can prevent people from sneezing.
C. People who eat an apple every day are less likely to catch a cold than people who do not
eat apples.
D. People who eat an apple every day recover from flu in shorter time than people who do
not eat apples.

12. Which of the following statements about minerals is incorrect?

A. Sodium is needed in plants for forming chlorophyll.
B. Calcium is needed in humans for muscle contraction.
C. Minerals are inorganic substances.
D. Nitrogen is needed in humans for muscles contraction.

13. Which of the following statements about sugars is correct?

A. Maltose is insoluble in water.
B. Fructose is reducing sugar.
C. Galactose can be hydrolysed.
D. Sucrose is the building block of starch.

14. Which of the following combinations of compounds are made up of the same elements?
A. glycerol and lactose
B. starch and protein
C. amino acid and nucleic acid
D. fatty acid and phospholipid

15. Which of the following process does NOT involve enzymes?

A. Sucrose dissolves in water.
B. Starch converts into maltose.
C. A protein stain dissolves in water.
D. Glucose converts into maltose.
 S3/Bio/AE/P.5

Section C : Complete tables (10 marks)

1. Metabolism (7 marks)

Anabolism (a)
Definition (b) Breaking-down reactions in which
complex molecules are broken down
into simpler molecules.
Energy change (c) (d)
Example Synthesis of glucose from
carbon dioxide and water during (e)
photosynthesis

2. Functions of water in different situations. (3 marks)

Situation Function of water

Complex food molecules are broken down during digestion. As a reactant in hydrolysis
Plant cells become turgid when they are full of water. (f)
(g) As a cooling agent
Blood carries nutrients, oxygen and metabolic waste around (h)
the body.

Section D: Short Questions. (18+2 marks)

1. Why is a leaf regarded as an organ but not a tissue?

2. State the function of condenser in the microscope.

3. Give two advantages of using a light microscope over an electron microscope to observe cells.

4. Give two functions of placing a cover slip over the slide?

5. Explain why red blood cells swell and burst when placed in distilled water.

6. Explain why proteases are used in making baby food.

7. What are the functions of nucleic acids?

8. Write a word equation to show the breakdown of sucrose into its monosaccharides.
Name the monosaccharides formed.

9. What is the importance of diffusion to organisms?

 S3/Bio/AE/P.6

Section E : Structural Questions. (24+1 marks)

1. The diagram below shows the structure of an animal cell.

A
C

(a) What is organelle D?

Explain why these organelles are abundant in liver cells. (3 marks)
(b) The length of organelle D in the diagram is 10 mm. However, the actual length of organelle
D is only 5 μm.
Calculate the magnification of this diagram. Show your working. (2 marks)

2. A student observed that plants grow well in sunny areas and grow poorly in shady areas.
He made a hypothesis and then carried out an experiment to test whether light is required for
plants to grow well.

(a) State the hypothesis made by the student. (1 mark)

(b)* Suggest how the experiment could be carried out. (3+1 marks)
(c) State one control variable in this experiment. (1 mark)
 S3/Bio/AE/P.7

3. An experiment was set up to investigate the effect of temperature on enzyme activity.

1cm3 of amylase solution and 4 cm3 volume of starch solution in separate test tubes were put
into water bath of different temperature. Allow the test tubes to stand in the water baths for five
minutes. For each beaker, take out the test tube that contains starch solution and pour into the
test tube that contains amylase and mix well. Timed how long it took for all the starch to be
broken down at different temperatures. The rate of reaction at each temperature was then
calculated.

Temperature (ºC) 4 20 40 100

Reaction rate
2 9 18 0
(arbitrary units)

(a) What is the independent variable in this experiment? (1 mark)

(b) Why is it necessary to keep the amylase solution and starch solution in their corresponding
water baths for five minutes before mixing them together? (2 marks)

(c) Briefly describe how you would determine all the starch has been broken down by amylase
in this experiment. (2 marks)

(d) According to the results, describe and explain the effect of temperature on amylase activity.
(4 marks)
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4. The schematic diagrams below represent different types of organic molecules and how they
can be joined to form more complex molecules.

condensation

different types of molecules P polypeptide

condensation

molecules Q polysaccharide

(a) Identify molecules P. (1 mark)

(b) Explain how the nature of molecule P and the way they are joined together lead to the
numerous functions of proteins in organisms. (2 marks)
(c) State one function of proteins in organisms. (1 mark)
(d) The type of polysaccharide formed from molecules Q shown above is a structural
component of plant cell walls. Name this polysaccharide. (1 mark)

***End of Question Paper***

 S3/Bio/AE/P.9

S3 A / B / C / D No. _____ Mark : ________/ 100

Section A : Fill in the blanks (2 mark @; 30 marks)
1(a) ethanol 9(i) hydrophobic

2(b) cellulose 10(j) flagella

3(c) optimum 11(k) shock-absorber

4(d) pseudopodia 12(l) carboxyl

5(e) concentration gradient 13(m) triglyceride

6(f) plasmolysed 14(n) circulatory

7(g) shorter 15(o) vascular

8(h) Channel protein

Section B. Multiple Choice (1 mark@;15 marks)

Choose the best option and put a tick () in the correct box provided.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
A    
B  
C      
D   

Section C : Complete table (10 marks)

a catabolism f Providing support to young seedlings and
non-woody plans.
(1) (1)

b Building-up reactions in which g We sweat a lot on hot days.

complex molecules are synthesized
from simpler molecules. (1)
(2)

c Energy is absorbed h As a transport medium.

(1) (1)

d Energy is released

(1)

e Breakdown of glucose into carbon

dioxide and water during respiration.
(2)
 S3/Bio/AE/P.10

Section D : Short Questions (18 + 2 marks)

1. A leaf is composed of several types of tissues, such as the epidermal tissue, vascular bundles
and mesophyll tissue.

2. Function of condenser in the microscope: : It concentrates the light reflected from the mirror on

the specimen / slide.

3.  Light microscopes can be used to examine living cells and their life processes.
 It is easier to use a light microscope than an electron microscope.
The images produced by a light microscope are in colour (since white light is usually used
as the illumination source), whereas electron microscopes do not produce images in colour

4.  To preven the objective from touching the specimen.

 To prevent the specimen from drying up.

To flatten the specimen.

5. The water potential of distilled water is higher than that of the red blood cell (i.e. distilled
water is hypotonic to the cells)
Water enters the red blood cells by osmosis, causing them to swell and finally bursts.

6. Proteases pre-digest some of the proteins in the food so that they can be more easily digested
and absorbed by babies.

7. Functions of nucleic acid : It play important roles in heredity and protein synthesis.

8.
Sucrose / invertase
sucrose + water glucose + fructose

9. Importance of diffusion:  It is important for the exchange of substances between cells and
their environment.
 It is responsible for the distribution of substances within the cytoplasm.

Section D effective communication : _____ / 2 marks

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Section E : (24 + 1 marks)

1.(a) Organelle D : mitochondrion

It is the site of cellular respiration to release energy. (1)

Liver cells are metabolically actve and need a lot of energy. (1)

(b) The magnification of the diagram is 2000. (1)

10mm = 10000 µm

Magnification = 10000 µm ÷ 5 µm = 2000 (1)

2. (a) Hypothesis : Light is necessary for the growth of plants. (1)

(b)* Place 3 seedlings into a conical flask containing nutrients solutions. (1)

Place the conical flask under bright light for a week. (1)

Set up a control by placing the same set-up in darkness. (1)

effective communication : _____ / 1 mark

(c) Control variable : species of plant / (1)

age of the plant /
volume of nutrient solution
concentration of nutrient solution /
temperature
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3. (a) Independent variable : temperature (1)

(b) To ensure that both solutions reach the required temperature before any reaction start. (2)

(c) At two minutes intervals, withdraw a drop of mixture to an iodine drop on the spot plate with
a clean dropper. (1)
Note the disappearance of blue-black colour in the spot plate and record the time taken for
the disappearance. (1)

(d) At low temperature, enzymes are inactive. (1)

As temperature rises, the substrate and enzyme molecules have more kinetic energy and they

move faster. (1)

They collide more often with one another and so enzyme-substrate complexes are more

likely to be formed. Rate of reaction increase. (1)

When the temperature increases beyond the optimum temperature, enzyme molecules vibrate

too vigorously that its shape starts to change, and so do the shapes of the active sites.

The enzymes are denatured. The rate of reaction decreases /stops. (1)

4.(a) Molecule Q : amino acid (1)

(b) Organisms use 20 types of amino acids / molecule P to make proteins.

They may join in different combinations to form polypeptide. (1)

The varying sequences and lengths of amino acids in polypeptide gives rise to protein

molecules of different shapes which serve different functions. (1)

(c) Function of protein in organisms : Enzymes / antibodies / hormones / component of

cytoplasm and membrane / source of energy (1)

(d) The polysaccharide is cellulose. (1)

******End of Answer Sheet*****