Sample Question Paper Class-Xii Bio-Technology (045) SESSION 2019-20
Sample Question Paper Class-Xii Bio-Technology (045) SESSION 2019-20
Sample Question Paper Class-Xii Bio-Technology (045) SESSION 2019-20
CLASS- XII
BIO-TECHNOLOGY (045)
SESSION 2019-20
SECTION A
1 Name any two scientists involved in designing the first recombinant DNA 1
molecule.
2 Write any two properties which can be improved through protein engineering 1
4 Which vector was used in the first cloning experiment involving mammalian cell? 1
OR
How does a modification enzyme protect its own DNA from digestion?
6. An enriched medium containing salts, glucose, proteins and vitamins was made 1
and a commercially available animal cell line was introduced. However, the cells
began dying. What could be the reason behind it?
OR
What is A in the flow chart?
8. Crystallisation is not required due to the advent of which of the following new 1
technique.
a) X-ray crystallography
b) NMR
c) Sanger’s method of protein sequencing
d) Edman’s method of protein sequencing
10. The single letter codes for Tyrosine and Asparagine are 1
a) N and Y
b) A and T
c) T and A
d) Y and N
11. The disease due to the deficiency of an enzyme Adenosine Deaminase (ADA) is 1
a) SCID
b) Thallasemia
c) Haemophilia
d) Mad cow disease
number begins to increase exponentially and a plateau phase, in which the
growth rate slows or stops due to depletion of growth factors and nutrients.
(ii). There is no increase in the cell concentration in the lag phase due to the 1
following reasons:
a) Exhaustion of the medium.
b) Space constraint
c) Both “a” and “b”
d) Acclimatization to the new environment.
(iii). In which phase of growth is the specific growth rate of animal cell calculated? 1
a) Log phase
b) Lag phase
c) Stationary phase
d) Decline phase.
(iv) A student adds antibiotic to the animal cell culture medium and still obtains the 1
same growth curve .The probable explanation for it will be:
a) Antibiotics add growth factors and hormones in the medium
b) Antibiotics provide serum for the growth of animal cells.
c) Antibiotics enhance the nutrient content of the medium.
d) Antibiotics don’t have any affect on animal cells
SECTION B
13 Differentiate between synthetic and complex medium used for microbial culture. 2
15 On the basis of the table given below, state your observations pertaining to the 2
organisation features of the organism.
19 In a variant of chymotrypsin, Asp 102 is replaced by Glu 102. Do you expect the 2
enzyme to retain activity? Schematically indicate the role of amino acid residues
participating in catalysis.
OR
Thalassemic patients produce excess alpha or beta subunits of haemoglobin
leading to impaired oxygen-binding capacity by their erythrocytes. How can it be
determined as to which subunit is produced in excess?
SECTION C
20 Recombinant insulin is produced at 100 mg/l by E. coli at a cell concentration of 1 3
g/l. Calculate the volume of reactor (size of the fermentor) needed to produce 1
kilogram of insulin in the following conditions:
(a) When the cell concentration is 1 g/l and insulin production is 100 mg /l.
(b) When the cell concentration is 50 g/l and insulin production is 100 mg /l.
(c) When the cell concentration is 50 g/l and insulin production is 500 mg /l
21 Schematically explain the formation of recombinant plasmid. 3
OR
Students of Class XII visited Microbial Type Culture Collection, Chandigarh and
observed microbial cultures of Providencia stuartii, Streptomyces albus and
Haemophilus aegyptus. Name the restriction enzymes obtained from them and
also specify their restriction sites.
25 How does the metagenomics approach help to identify novel genes present in 3
the environment? Explain the process.
OR
What is a pilot plant? Why is it necessary to validate a process in a pilot plant
before commercial scale production in a bioprocess industry?
26 Given below are few transgenic crops approved by US food and drug 3
administration along with the improved character. Name the genes A to F
introduced for the improved character.
SECTION D
27 Mutation is an alteration in any of the base of a DNA sequence sometimes 5
leading to a defective protein or prematurely terminated non-functional protein. It
can be spontaneous or induced. Diagrammatically explain how mutation can be
induced in a gene.
OR
A bacteriophage is known to infect E.coli with pili. How can it be modified to
serve as a suitable vector?
5
What are the major advantages of developing vectors based on such
bacteriophages?
29 Which two main methodologies are used for genome sequencing? Explain. 5
OR
Explain the diseases caused by single gene mutations following mendelian
inheritance specifying the genomic location, inheritance pattern and mutation.
Name any two diseases showing gene polymorphism with complex inheritance.
30 Diagrammatically show the cultivation of adult stem cells from bone marrow and 5
their differentiation into specialized cells. Name two scientists who established
the field of stem cell research. For which medical conditions, stem cells can be
used (Write any two)?