Automated Immunoassay System

HISCL-5000

Speed and Reliability

Matters

www.sysmex-ap.com
 HISCL-5000

Real Continuous System

Rapid Analysis
• High processing capacity with 200 tests per hour
• Time to First Result: 17 Minutes
Equipped with innovative measurement technology
that enables fast turnaround time of 17 minutes for all
parameters, laboratories can now be assured of meeting
their objectives of speed and throughput. Faster reporting
of results enable timely patient diagnosis and treatment.

Low Sample Volume

Required sample volume of 10 – 30µL for all parameters

Sample volume from pediatric and geriatric patients are no

longer a concern. HISCL-5000 can analyse all parameters
with small sample volumes, requiring only 10 – 30µL using
a dedicated disposable tip. It offers maximum assurance
for the patient and clinician.

HISCL-5000: Excellence Meets Innovation

A solution that answers your needs and enhances your
laboratory productivity.

HISCL-5000 is designed to provide additional value to

laboratories in terms of clinical requirements, operational
needs, and managerial demands. HISCL-5000 offers
you a smart laboratory system with a pipeline of unique
biomarkers to ensure your laboratory is prepared for new
Customised Configuration allows Flexibility and Integrated Solutions
clinical solutions.
Highly efficient workflow automation can be customised for the fastest Turnaround Time (TAT) and
utmost productivity.

Fast and flexible sample processing is achievable through optimised use of analyser capacity and
intelligent sample routing.

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 HISCL-5000

Excellent Usability

Continuous Loading System Membrane Filter Eliminates Carry-over

Twenty-four sets of assay reagents can be set up at A membrane is used to clean the nozzle for each sample
the same time. Two sets of common washing/cleaning dispensing, assuring a highly-reliable system to eliminate
reagents can be simultaneously loaded. There is an carry-over.
automatic switching feature that allows these reagents to
be replenished without interruption of sample analysis. Chemiluminescent Substrate CDP-Star
The reagent bottle cap has automatic opening and closing A measurement system with high sensitivity is achieved
mechanism that ensures stability of reagents. by adoption of Chemiluminescent substrate CDP-Star
that demonstrates high luminescence intensity through
User Friendly Interface its dephosphorylation by alkaline phosphatase. Strong
The 21-inch large colour touch screen display features an luminescence from CDP-Star is efficiently detected using
intuitive user interface design with large icons. Software an optical filter. The high sensitivity and wide measurement
functions support day-to-day operations with immediate range is key to low sample volume application.
access to information such as reagent levels and testing
progress status. Comparison of Detection Limit between Various
Chemiluminescent Substrates for Immunoassays
Effective Reagent Management System
Reagent information management using RFID (Radio
Frequency Identification) is adopted. Accurate data such
as expiry date and remaining tests will be provided.

Refrigerated Reagent Storage

The reagent setting part has a function to keep reagent
cool for 24 hours thus ensuring reagent stability. The
instrument wake-up function offers a hassle-free daily
startup at the scheduled time.

STAT Analysis Available

The STAT position located in the middle of the rack supply
allows urgent samples to be processed.

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 HISCL-5000

High Sensitivity
HISCL Hepatitis B Virus Surface Antigen (HBsAg) Assay Analytical Flow
The HISCL HBsAg is a 2-step competitive assay based on Chemiluminescence Enzyme Immunoassay (CLEIA) method
using CDP-Star chemiluminescent substrate.

Improved Reaction Efficiency at 42 ºC

Setting the entire reaction temperature at 42˚C increases the antigen-antibody encounters, therefore it increases the
efficiency of assay.

Reaction Flow (HBsAg Measurement)

Antigen-antibody Reaction Speed Up by Liquid Phase - Liquid Phase Reaction

R1: Biotinylated anti-HBs R2: Streptavidin-coupled The liquid phase - liquid phase reaction system is adopted for primary reaction. This increases the frequency of
monoclonal antibody (mouse) magnetic particle antigen-antibody reaction.
HBsAg in a sample Quick reaction in the liquid phase Trap to the magnetic particle
Avidin-Biotin reaction
Antigen

Antibody

Primary Reaction
Magnetic particle

Washing buffer (4 times wash) R3: ALP labeled anti-HBs

monoclonal antibody
(mouse)
MAGNET

Increased Specificity Through Background Reduction

Background influence is reduced by the improved efficiency of separation of reactants from unreacted materials
(B/F separation). Each wash cycle is performed 4 times.

Primary Bound/Free (B/F) Separation Secondary Reaction

MAGNET

MAGNET

MAGNET
Washing buffer (4 times wash) R4: Buffer solution

R5: Chemiluminescence
substrate CDP-Star

Magnetic Collection Washing Dispersion Magnetic Collection

Secondary B/F Separation

Luminescence intensity measurement

Measurement result output Wide-range Detection with an Optical Filter

Strong luminescence from CDP-Star is efficiently detected by using an optical filter. Highly sensitive and wide-
range measurement is therefore possible.

Enzymatic Reaction (Chemiluminescence)

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 Specifications Note

Measurement Principle Chemilumniescence Enzyme Immunoassay (CLEIA)

Number of Parameters Onboard Up to 24 parameters
Processing Capacity 200 tests per hour
Reaction Time Approximately 17 minutes (time from sample aspiration to measurement
result display)
Sample Capacity Up to 100 samples
Number of STAT Positions 1 sample
Sample Volume 10-30 μL
Sample Container Test-tube (Open) Diameter (Outside diameter): 13-16 mm
Height: 75-100 mm
Dead volume: The depth of the serum or plasma
component from the surface of liquid is 5 mm or more
Sample cup 4 mL conical cup
Dead volume: 0.15 mL
Reagent Management Function Assay reagents, Luminescence substrate set: RFID
Line washer, cleaning fluid, probe washer: barcode
Sampling Monitoring System Sample aspiration and sample abnormality monitoring via pressure sensor
Data Storage 100,000 sample results (maximum)
Quality Management L-J (Chart) or X-bar (Chart)
External Output RS232C, LAN
Power Supply Specifications Measurement part: AC 200-240 V
Compressor: AC 100 V Rated
Frequency: 50 Hz / 60 Hz
Printing System External printer
Accessory Handheld barcode reader
Options Drainage tank, indicator light, conveyance connection kit

Data
Main Unit Dimensions (W x D x H) Approximately 1,725 x 840 x 1,300 mm
(including the sampler) Weight Approximately 490 kg
Compressor Dimensions (W x D x H) Approximately 340 x 500 x 390 mm
Weight Approximately 28 kg

Caresphere XQC* -Successor of SNCS Online QC

Caresphere XQC Online QC is an inter-laboratory comparison tool that offers the possibility to compare internal QC results
of Sysmex analysers against the affiliated or global peer group of the same analyser model using the same lot number of
Sysmex QC material and measurement method. Caresphere XQC is a web-based system that is easy and convenient to use.
This system is unique because it integrates daily internal QC and interlaboratory comparison for statistical analysis and the
laboratory can view the processed statistical data through secure online access to Caresphere XQC.

* Caresphere XQC. The content of support services varies by region. A separate (paid) agreement is required to use this service.

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 Design and specifications may be subject to change due to further product development.

Sysmex Asia Pacific Pte Ltd Sysmex New Zealand Ltd Sysmex Vietnam Co., Ltd
Tel +65 6221-3629 Fax +65 6221-3687 Tel +64 (9) 630-3554 Tel +84 (028) 3997 9400 Fax +84 (028) 3997 9405
www.sysmex-ap.com www.sysmex.co.nz www.sysmex.com.vn

Sysmex India Pvt. Ltd Sysmex (Thailand) Co., Ltd Sysmex Vietnam Co., Ltd (Hanoi Branch)
Tel +91 (22) 6112-6666 Tel +66 (2) 032-2536 Fax +66 (2) 116-5396 Tel +84 (024) 3776-7020 Fax +84 (024) 3776 7022
www.sysmex.co.in www.sysmex.co.th www.sysmex.com.vn

PT Sysmex Indonesia Sysmex Philippines Inc. Sysmex Australia Pty Ltd

Tel +62 (21) 3002-6688 Fax +62 (21) 3002-6699 Tel +63 (2) 621-2460 Fax +63 (2) 621-2432 Tel +61 (2) 9016-3040
www.sysmex.co.id www.sysmex.com.ph www.sysmex.com.au

Sysmex (Malaysia) Sdn Bhd

Tel +60 (3) 5637-1788 Fax +60 (3) 5637-1688
www.sysmex.com.my
SAP/IMMUNO/HISCL-5000/04032021/V4.0