Triladyl® & Biladyl®

Media for Bovine Semen Freezing

:::::::::::: Proven Standard for Bovine Semen Production.
Based on TRIS Buffer. Requires fresh egg yolk to add.

Your benefits
• Elaborate formula, based on TRIS

• Since over 30 years internationally established

• Very constant, optimal freezing results at a wide range of dilution ratios

• CSS certified version without antibiotics available

(Triladyl CSS® and Biladyl®)

• Produced under Minitube GMP production standard

Triladyl®, 250 g with antibiotics GTLS according

to the EC Directive 88/407 REF. : 13500/0250

Triladyl® CSS, 200 g without antibiotics, according

to CSS requirements REF. : 13500/1200

Biladyl®, fraction A, 49 g REF. : 13500/0004

Biladyl®, fraction B, 250 g REF. : 13500/0006

Composition ::::::::::::
Antibiotic Supplement GTLS, for 2-step extender,
according to CSS protocol REF. : 13500/0005 Triladyl®, Triladyl® CSS and Biladyl® contain TRIS, citric
acid, sugar, buffers, glycerol, purest water and antibiotics
Antibiotic Supplement GTLS, for 1-step extender, according to the EU Directive 88/407 (Tylosin, Gentamicin,
Subject to modifications, errors excepted. Illustrations similar. 08/2012

according to CSS protocol REF. : 13504/9000 Spectinomycin, Lincomycin). Triladyl® CSS contains no
antibiotics.

w w w. m i n i t u b e . d e
:::::::::::: Industry standard Selected scientific publications ::::::::::::

Since Triladyl® and Biladyl® have been introduced to the market, they Christensen P., Boelling D., Pedersen K.M., Korsgaard I.R., Jensen.
have established themselves as the classic extenders in bovine semen (2005): Relationship between sperm viability as determined by flow
production. Triladyl® and Biladyl® are also used successfully with cytometry and nonreturn rate of dairy bulls. J. Androl., Jan-Feb; 26
ejaculates of several other species, e.g. ovine, caprine as well as with (1): 98-106
many exotic species.
Dumont P. (2002): Adjusting the number of spermatozoa in mini-
straws by determination of the operative volume of bovine
semen. Theriogenology, Apr 1; 57(6): 1743-54
:::::::::::: Efficient production protocols
Herold, F.C., Gerber, D., Aurich, J.E. (2002): Influence of homolgous
For the preparation of Triladyl® and Biladyl® distilled water and seminal plasma on bovine epididymal semen frozen with Triladyl® or
250g fresh egg yolk are added to the concentrate. The ejaculates can AndroMed®. Wiener Tierärztliche Monatsschrift, 90, 8–61.
be diluted with a large range of variation in dilution ratio, without
influencing the fertility results. Triladyl® and Biladyl® are being used Nabiev, D., Gilles, M., Schneider, H., Mahabir, E., Koll, H., Schellander,
successfully with low concentration dilution (below 15 million sperm H. & K. (2003): AndroMed® versus Triladyl® - influence on functional
cells per dose), as well as for bovine ejaculates with low density. in vitro fertility parameters and IVP of frozen thawed bovine semen.
Vet. Med. Austria 90, Suppl. 1.

Herold, F.C., Gerber, D., de Haas, K., Noethling, J.O., Cooper, D., Theu-
nisen, W., Spillings, B. (2003): Comparison of three different media for
:::::::::::: Production standard and QC freezing epididymal sperm from African buffalo (Syncerus caffer)
and influence of equilibration time on the post-thaw sperm quality.
Raw materials are produced according to GMP and DIN ISO 9001 norms, Theriogenology, 59, 393.
and are certified after Ph Eur, BP or USP standards. They are tested
according to international valid quality guidelines concerning the Herold, F.C., Aurich, J.E., Gerber, D. (2003): Epididymal sperm from Af-
testing of pharmaceutical substances and meet these requirements. rican buffalo (syncerus caffer) can be frozen successfully with Andro-
Each single component of each extender batch is tested chemically, Med® and with Triladyl® but the addition of bovine seminal plasma is
physically and spermatologically under specialized veterinarian detrimental. Therioenology, in press
supervision for their adequacy for the semen conservation. Analysis
certificates and testing protocols constitute full traceability. Szasz F., Gabor G., Solti L. (2000): Comperative study of different me-
Mixing and bottling of the extender is performed under GMP thods for dog semen cryopreservation and testing under clinical
conditions in controlled atmosphere and documented with weighing conditions. Acta Vet Hung, 48(3): 325-33
records. The complete mixture is tested again chemically and physically
for spermatologic adequacy as well as under practical conditions for Garde J.J., Soler A.J., Cassinello J., Crespo C., Malo A.F., Espeso G., Go-
the suitability for semen conservation under specialized veterinarian mendio M., Roldan E.R. (2003): Sperm cryopreservation in three spe-
supervision, and by independent organizations. cies of endangered gazelles (Gazella cuvieri, G. dama mhorr, and G.
dorcas neglecta). Biol reprod., Aug; 69(2): 602-11. Epub 2003 Apr
16

Janett F., Keo S., Bollwein H., Hässig M., Thun R. (2004): Comparison
of AndroMed®, Bioxcell and Triladyl® extender for cryopreservation of
:::::::::::: Certificates bull semen. Conference on Physiology and Pathology of Reproduc-
A general quality certificate as well as a batch certificate are available tion; Zürich, February 2005
upon request.
Janett F., Fuschini E., Keo S., Thun R. (2005): Comparison of Andro-
Med® and TRIS-egg yolk extender for cryopreservation of buck se-
men. ESDAR Conference, Murcia, Sept. 2005

Complete publication list available on request.

w w w. m i n i t u b e . d e