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    007 - Asma Saparas (SNF Lab 11) - BLAST

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    asad shafiq
    The document analyzes the first 5 alignments of the human insulin nucleotide sequence from NCBI against other sequences using BLAST. It calculates the number of identities, mismatches and gaps for each alignment. The percentages of identities range from 100% for the first alignment down to 85% for the fifth alignment, indicating close matches between the query sequence and subjects.

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    007 - Asma Saparas (SNF Lab 11) - BLAST

    Uploaded by

    asad shafiq
    35 views6 pages
    The document analyzes the first 5 alignments of the human insulin nucleotide sequence from NCBI against other sequences using BLAST. It calculates the number of identities, mismatches and gaps for each alignment. The percentages of identities range from 100% for the first alignment down to 85% for the fifth alignment, indicating close matches between the query sequence and subjects.

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    Bioinfomatics The use and importance

    Original Title

    007_Asma Saparas (SNF lab 11)_BLAST

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    The document analyzes the first 5 alignments of the human insulin nucleotide sequence from NCBI against other sequences using BLAST. It calculates the number of identities, mismatches and gaps for each alignment. The percentages of identities range from 100% for the first alignment down to 85% for the fifth alignment, indicating close matches between the query sequence and subjects.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    35 views6 pages

    007 - Asma Saparas (SNF Lab 11) - BLAST

    Uploaded by

    asad shafiq
    The document analyzes the first 5 alignments of the human insulin nucleotide sequence from NCBI against other sequences using BLAST. It calculates the number of identities, mismatches and gaps for each alignment. The percentages of identities range from 100% for the first alignment down to 85% for the fifth alignment, indicating close matches between the query sequence and subjects.

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    © All Rights Reserved
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    Asma Saparas (SP19-BSI-007)

    LAB ACTIVITY 11

    Q1: Copy the nucleotide FASTA sequence of the human insulin from this
    link:

    https://www.ncbi.nlm.nih.gov/nuccore/BC005255.1?report=fasta

     Use BLAST to view its alignments.


     Attach the screenshots of its first 5 alignments.
     Count and calculate their total number of identities (matches), mis-
    matches and gaps.
    Asma Saparas (SP19-BSI-007)

    ALIGNMENT 1

    Total no of identities
    = 495 /495

    (100%)
    Gaps = 0

    Mismatches = 0

    Gaps +Mismatches = 0

    Total number – the total


    number of gaps & mis-
    matches

    495-0= 495

    Percentage=495/495x100

    =100%
    Asma Saparas (SP19-BSI-007)

    ALIGNMENT 2

    Total no of identities

    = 467 /469 (99%)


    Gaps = 0

    Mismatches = 2

    Gaps +Mismatches = 2

    Total number – the total


    number of gaps & mis-
    matches

    469-2= 467

    Percentage=467/469x100

    =99%

    Mismatches
    Asma Saparas (SP19-BSI-007)

    ALIGNMENT 3

    Total no of identities

    = 231/240 (96%)
    Gaps = 0

    Mismatches = 9

    Gaps +Mismatches = 9

    Total number – the total


    number of gaps & mis-
    matches

    240-9=231

    Percentage=231/240x100

    =96%
    Asma Saparas (SP19-BSI-007)

    ALIGNMENT 4

    Total no of identities

    = 341/397 (86%)
    Gaps = 10

    Mismatches = 46

    Gaps +Mismatches = 56

    Total number – the total


    number of gaps & mis-
    matches

    397-56=341

    Percentage=341/397x100

    =86%
    Asma Saparas (SP19-BSI-007)

    ALIGNMENT 5

    Total no of identities

    = 367/431 (85%)
    Gaps = 10

    Mismatches = 54

    Gaps +Mismatches = 64

    Total number – the total


    number of gaps & mis-
    matches

    431-64= 367

    Percentage=367 /431x100

    =85%

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