Datasheet 239702
Datasheet 239702
Datasheet 239702
Overview
The bile acid assay is based on an enzymatic cycling method in the presence of NADH and a
chromophore. The reduction of the chromophore produces a stable colorimetric product the
absorbance of which can be followed kinetically at 405 nm on a multi-well spectrophotometer.
This absorbance is directly proportional to the amount of total bile acids in the sample.
Notes This product is manufactured by BioVision, an Abcam company and was previously called K209
Total Bile Acids (TBA) Assay Kit (Colorimetric). K209-100 is the same size as the 100 test size
of ab239702.
Properties
1
Components 100 tests
NADH 1 vial
Relevance Bile Acids (BA) make 67% of the total composition of Bile. They are 24-carbon steroids
generated during cholesterol metabolism. They form conjugates with either glycine or taurine to
form bile salts. Five of the BAs account for more than 99% of the total population found in
biofluids. The average composition in healthy individuals includes conjugates of cholic,
chenodeoxycholic, deoxycholic and lithocholic acids. Bile acids are critical due to their ability to
solubilize lipids by forming micelles with cholesterol, and fatty acids. Their synthesis is not only
critical for the removal of cholesterol from the body abut they are also needed for proper uptake of
dietary lipids into the small intestine.
Images
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30 µL of each undiluted sample was assayed following the kit
protocol. Bile Acids concentrations are: Serum (in µM): A: 3.56 ±
0.41, B: 2.41 ± 0.27, C: 1.63 ± 0.17, D: 1.34 ± 0.25, Urine: 0.16 ±
0.02 µM/mM Creatinine.
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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