CS 400 Service Manual New

Specification Model: CS-400
Product composition：The analytical part (host), operation part (computer system), the
result
output part (printer), accessories and consumables.
Product applicable scope: used for quantitative analysis of serum, plasma, urine,
cerebrospinal fluid and other clinical chemical constituents of sample.
1-1-2 The Front of Analyzer
① ②
① Machine model logo
②Upper cover
③Left front door
④ Right front door
③ ④
1-1-3 Front Open Picture of Analyzer
① SIP (reference), injection pump
② DIL (Diluted Release) injection pump
③ IS (internal standard), injection pump
④ CS-alkaline cleaning liquid box
⑤ S injection pump
⑥ R2 injection Pump
⑦ R1 injection pump
①②③④ ⑤ ⑥⑦
1
1-1-4 The back of analyzer
① Power entrance
② RS232 interface
③ left back cover board
④ cooling fan
⑤ light liquid outlet
⑥ right back cover board
⑦ purified water entrance
⑧ concentrated liquid waste outlet

① ② ③ ④ ⑤⑥ ⑦ ⑧⑨
⑨ concentrated waste liquid level sensor interface
1-1-5 The Top of Analyzer

① ② ③ ④ ⑤⑥ ⑦ ⑧
① sampling mechanism
② reaction cup cleaning mechanism
③ reaction disk mechanism
④ reaction bath liquid detection

⑤ R1 stirring mechanism
⑥ R1 reagent adding mechanism
⑦ probe cleaning reagent groove
⑧ R1 reagent disk
⑨ sample disk rotating indicator
⑩ sample disk inner refrigerated cover
⑾ sample disk
⑿ R2 reagent adding mechanism ⑨ ⑩ ⑾ ⑿⒀ ⒁
⒀ R2 stirring mechanism
⒁ R2 reagent disk
2
1-1-6 The Right of Analyzer
① Analytical unit switches
(not including refrigeration power supply)
② refrigeration power supply indicator（green）
③ main power supply（breaker）
④power indicator（red）
①② ③ ④
3
1.2 Analytical Unit Composition
CS-400 auto-chemistry analyzer working speed means the one at which it reaches
constant speed 400 tests / hour of single / double-reagent item, whose working period is 9
seconds. Instrument overall structure adopts the "4 -disk + three-probe + two-stirring rod",
specifically, a sample disk, one reaction disk, two reagent disks, 2 reagent probes for
adding R1 and R2 respectively, a sample probe for sampling, 2 stirring rods for mixing
R1,R2 respectively. "Grating + diode array" approach is adopted in optical measurement
mechanism for real-time optical collection of reaction cup. The rinsing mechanism 7-stop
11-step automatically rinsing the reaction cup is carried out in test process.
1-2-1 The Structure of Analyzer
Sample disk Barcode window

1-2-2 Sample Unit
115 sample positions，including：
Routine sample position： 50
STAT sample position ： 20
Standard solution position: 34

QC position： 8
Cleaning liquid position： 3
Barcode window： 1
4
1-2-3 Reagent Unit
Reagent position：45×2
R1disk：only for R1and R4
R2disk：only for R2 and R3
Position 45 of two reagent disk:
Only for phosphor-free cleaning liquid
Reagent bottle volume：70mland 20ml
1-2-4 Reaction Unit

Reaction cup Reaction disk
Reaction cup：120，optical path: 6mm
20×6 sets hard optical plastic
cup
Incubation bath
Digital liquid sensor
7-stop 11-step rinsing of colorimetric cup
5
Stirring mechanism probe mechanism
1-2-5 Probe and Stirring Unit
Probe mechanism：3
1sample probe, 2 reagent probes
High-precision digital liquid detector
Stirring mechanism：2
High-speed hollow cup motor
Surface high-intensity Teflon coating
1-2-6 Precise Distributing Pump （Injection Pump）
① ② ③
① ISE unit：3（SIP，DIL，IS）
② Colorimetric unit：3 （sample，reagent R2，reagent R1）
③ Colorimetric cup original rinsing liquid box
6
① ②
1-2-7 Control unit 1
① circuit panel boxes: 6 panels
Order: from left to right
Reaction disk control panel
Sample disk control panel
Reagent R1 control panel
Reagent R2 control panel
ISE control panel（Optional）
Main control panel
②Switching Power Supply Box：
Order: from left to right
＋12V，－12V（simulation）
＋12V（lamp）
③ ④ ⑤ ⑥ ⑦ ⑧ ⑨
＋5V（digital circuit）
＋24V（motor, valve）
③ Halogen Lamp Power Interface
④ Circuit panel box power supply interface
⑤ 24VPower Control Interface
⑥ halogen lamp power supply control interface
⑦ switching power supply (except +12 V (lamp))
⑧ communication control interface
⑨ fan interface
7
① ②
① Connection adaption and the status indicating board
② AC electrical driver panel
Semiconductor refrigeration systems:：
Control panel (with status indication)
+5 V panel power
＋13.5V Power Supply
cooler
Cooler (with 4 fans）
Cooler display:
E1 refrigeration temperature, E2 machine internal

temperature C1－C4 4 Cooler current
AC circuit breaker: 5
Fan (4A)
4 communication pump (6A)
2 Heater (10A)
4 Switching Power Supply (10A)

Refrigeration system (10A)
Isolating transformers
Arrester panel
8
1.3 Function Overview
Main work flow：
1. All mechanical moving parts unit initialization.。
2. 4 times water blank measure is implemented after the fifth time rinsing of 7 times
automatically rinsing of reaction cup.
3. Sample assimilates quantitive sample when it descents to sample disk after the
sample disk rotates to designated sampling position.
4. After 7-stop 11-step cleansing, reaction cup stops at the sampling position, and
sample probe rotates to reaction disk and descends to reaction cup to discharge
it, and sampling finished.
5. RI reagent probe descents to R1disk to assimilate quantitive reagent when the

reagent disk rotates to designated position.
6. R1 reagent probe rotates to reaction disk and discharges reagent R1 when the
reaction cup finishing sampling rotates to R1sampling position.
7. Reaction cup finishing sampling R1 is stirred immediately when it rotates to

R1stirring position.
8. sample ＋R1 reagent are reacting or temperatured.
9. If it is double item test, R2 reagent disk rotates to the designated R2 reagent

position and R2 reagent probe descends to R2 reagent disk to assimilate
quantitive reagent after a set period (one minute plus 30 seconds).
10. The R2 reagent probe discharges the R2 into reaction cup when it rotates to
reaction disk after reaction cup rotates to R2 sampling position.
11. Finishing sampling R2 reagent, reaction cup is stirred after its one circle (2
patches) rotation.
12. Reaction cup carries out the collection of absorbance data when it passes the
optical unit in every period.
13. The process of sampling R3,R4 reagent and sampling R1, R2 reagent.（the
same system for R1、R4，the same system for R2、R3）
14. R3 sampling position is used frequently instead of R2 sampling position for

double reagent item test so as to prolong the temperaturing time of sample and
R1 reaction liquid for 5 minutes. 10 minutes elapses in the process from
sampling to adding R4.
15. The reaction cup finishing reaction is rinsed automatically when passing the
rinsing unit, and 15minutes elapses since sampling to rinsing.
9
Table 3-1-1 Main Function of Each Unit
Name main function
Sample probe unit Execute sample assimilation and discharge of all biochemical
items and ISE items
Sample disk unit Total 115 sample positions for carrying all test samples,
standard solution and QC liquid.
R1probe unit Execute assimilation and discharge of all biochemical items

R1, R2 reagent.
R2 probe unit Execute assimilation and discharge of all biochemical items

R1, R2 reagent.
Reagent R1 disk total 115 reagent positions for carrying R1, R4 test reagent
unit and detergent
Reagent R2 disk total 45 reagent positions for carrying R1, R3 test reagent and
unit detergent
Reaction disk unit Total 120 reaction cups used as container of reaction and
colorimetry test.
Reagent R1 stirring Stirring when reagent R1, R4are added into reaction cup.
unit
Reagent R2 stirring Stirring when reagent R2、R3 are added into reaction cup.
unit
Optical system Measure 12 wavelength absorbance by grating system

groupware
Auto-rinsing unit Rinse reaction cup automatically by 7-stop 11-step
ISE unit（optional） Carry out ISE measurement (K、Na、Cl）
barcode Total 3 for scanning reagent bottles in the two reagent disk
and sample cups in sample disk.
10
Chapter 2 Instrument Installation
2.1 Installation Space Requirement：
To make sure the space of maintenance, operation and repair, please follow the instruction
as below:
● Space between left (right) side of analyzer and the wall should ≥50cm
● Space between rear board of analyzer and the wall should ≥50cm
● Space in front of analyzer should≥100cm
● Make sure there is enough space for waste device and purified water
equipment.
2.2 Power supply requirement：
● Power supply: ~220V, 50Hz
● Power: 2000 VA
● Circuit breaker: 250V, 20A

Instrument is equipped with a three core electrical wire, red wire is live line, blue wire is zero line,
and yellow green wire is ground lead as figure 1 shows.
Figure 1
A well grounded power supply socket is a must. (Socket at least with one 20 A and three 5A).
Large electrical appliance such as air condition, refrigerator, even cannot use the same
electrical wire as analyzer.
! Warning:
△
¾ Incorrect earthing may cause electric shock or instrument damage.

¾ Input voltage should conform to requirement. 6KVA-line UPS power supply is advised.
11
2.3 Environment requirement
● Working environment: 15℃～32℃

● Relative humidity: 40％～85％
● Atmospheric pressure: 76kPa～106kPa
● Environment should be with no dust, mechanical vibration, and noise source and
power interference
● Do not put the analyzer in the vicinity of brush motor, flicker fluorescent tube and
other constant on-off electrical equipment.
Hard and flat enough the ground should be to stand the instrument.
● Avoid direct sunlight, do not put the analyzer in front of heat source and wind source
Keep good ventilation of the instrument.
! warning：
△
¾ Normal running and accuracy of result can not be guaranteed if instrument works beyond
the requirements mentioned above. Please use air conditioner if the temperature or
humidity can not meet the requirement above.
¾ The heat generated in the work process by the instrument will be emitted the rear of the
instrument, so good ventilation should be kept well and ventilation equipment can be
adopted if necessary, but direct air current is avoided, or inaccuracy of instrument test may
be caused.
4.2 2.4 Purified water equipment Requirement：

① water should be obtained from tap water pipe
② water conductivity should within 1uS/cm
③ water supply volume should reach 40L/h or more
④ The hydraulic pressure should within 49-343 Kpa
2.5 Instrument Installation Flow：
¾ Make sure the installation place, space, electrical environment, installation room
temperature and purified water equipment can conform to requirements
¾ Make sure instrument installation tools needed are complete and reagent and QC liquid are
enough.
¾ Please check the prepared items according to packing list when open the package; please
write them down on the check report if any missing.
12
¾ Place instrument in applicable position, and mount with computer host, display and printer.
¾ Connect water supply and waste liquid outlet equipment.
¾ Adjust instrument level, and check whether the injection pump wires are loose or not after
open the left and right cover board of instrument.
¾ Infuse CS-alkaline detergent into instrument rinsing box, and infuse CS-anti-bacterial
detergent into the 45th position of R1, R2 reagent disks.
¾ Replenish cooling system water tank with purified water.
（a）Switch off the main power
（b）Demount instrument left front cover board as figure 2 shows:
（c）Unplug the cork of the two hoses connecting to cooling system water tank as figure 3
shows：
High water level hose
Low water level hose
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（d）Infuse purified water into low water level hose till the purified water flows out of the
high level hose.
（e）Switch on the main power. After several minutes, continue to infuse purified water
into low water level hose till the purified water flows out of the high level hose again,
requiring 3L water.
（f）Replug the rubber cork and mount the left front cover board of the instrument.
¾ Check whether power supply and data wires are connected.

¾ Mount reagent probe, sample probe, reaction cup.
¾ Check the up and down flexibility of reagent probe and sample probe.
¾ Get through pure water machine, computer host and display and analytical unit power
supply, and enter CS auto-chemistry analyzer systematic application software. Initial user
name: 001, initial password: 001.
¾ After enter software, follow the steps below in “Maintenance” interface.
（a）Injection pump exhaust
Execute injection pump exhaust to expel air in pipeline.
（b）Cleaning liquid pipeline exhaust
Executing irrigation cleaning liquid pipeline exhaust is infusing cleaning liquid into
pipeline to expel air in pipeline.
（c）Reagent probe horizontal check
Make sure reagent probe is right above reaction cup, rinsing groove and reagent bottle.
（d）sample probe horizontal check
Place a standard cup at position C8 in the sample disk outer track, middle track and
inner track respectively, and make sure the sample probe is above reaction cup, rinsing
groove, standard cup by implementing sample probe horizontal check.
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（e）Stirring rod horizontal check
In order to make sure the stirring rod is above the reaction cup, rinsing groove.
（f）Mechanical movement check
Execute 20 times mechanical movement checks to make sure whether the washing
block of rinsing mechanism nozzle abrases the reaction cup or not and each
mechanism runs normally or not.
（g）Rinse reaction cup+ ISE
Select rinsing reaction cup in “Maintenance” interface, and execute rinsing reaction cup
+ ISE if ISE equipment is collocated.
（h）Light quantity check
Light quantity result should be attached to installation check report with its value no
more than 18000.
（i）Cup blank test
No. 1 cup blank value should be within 18000, and 2-120 reaction cup check value
should be within 18000 ±800.
2.6 Clinical item test
Edit chemical parameters; register reagent info.; testing rate assay ALT, point assay,
two-point rate BUN; calculate the difference of parameter and the result of test should be
attached to installation check report.
2.7 Train Medical Personnel。
2.8 Fill the Installation Check Report Detailedly.
15
Chapter 3 Performance and Test Flow
3.1 Main Performance Index
3.1.1 Instrument standard specification
Performance Index standard specification
Grating rear spectrophotometry system,

Simultaneous photometric processing of 12
Wavelength wavelengths within the range：340 、380 、405 、
range
450 、480 、505 、546 、570 、600 、660 、
700 、750nm
Wavelength
±2nm
precision
characteristics Reaction
37℃±0.1℃
temperature
Simultaneously testing 88 colorimetric items and 3

Test item
ISE items at most
Measuring
Rate assay ,end-point assay, 2-point assay.
method
Measuring Constant speed, 400 tests/ hour ( 640 tests/ hour

speed with ISE)
Total 115 positions(routine sample: 50, calibrator:

Sample disk
Sample system 34, STAT: 20, QC liquid: 8, cleaning liquid: 3) in
and position
inner, middle, outer circle.
Sample Serum, plasma, Urine, cerebrospinal fluid, ascites

type and other body fluids
16
Sample
2～35ul，0.1μl Incremental
volume
Test tube Φ10mm×75mm 、 Φ10mm×100mm 、
Sample cup Φ13mm×75mm、Φ13mm×100mm(±1 mm)

specification
Standard cup Φ14mm×37mm(±1 mm)
Remaining
sample More than 100μl
volume
Appropriative probe ， with liquid detector,

Sample
bump-proof function and probe clot detection
probe
function.
Sample
Inner, outer wall rinsing
probe rinsing
Sample liquid Digital liquid detecting, integration with sample

level sensor probe
Type: code 128 、 code 39 、 code 93 、 12of5 、

UPC/EAN
Size：width: 8～12mm，valid length within 40mm,

start blank and finish blank within 3mm when
cutting.
Barcode
info. Sticking requirement: the lower edge of barcode
should be within 15 mm ～20mm away from the
test tube bottom to make sure right reading
barcode, and make sure the barcode is aligned
with sample position gap when putting the test
rack.
R1 and R2 reagent disks with two refrigerator,

Reagent system Reagent disk, adopting a semiconductor refrigerating, 45 reagent
reagent positions for each reagent disk (the 45th fixed for
position CS- anti-bacterial phosphor-free cleaning
liquid.
Reagent
20～350ul，1μl (incremental)
volume
Reagent
bottle 20mL 、70mL
specification
Reagent
remaining Reagent liquid volume: more than 3 ml
volume
17
Reagent R1、R2: using special probe respectively with liquid
probe level detector and bump-proof function.
Reagent
Inner and outer wall rinsing
probe rinsing
Reagent
storage All reagent should be stored at 5℃～15℃
temperature
Reagent liquid Digital liquid level detecting, integration with

level sensor reagent probe
type：code 128
size: Size：width: 8～12mm，valid length within

40mm, start blank and finish blank within 3mm
when cutting.
Barcode info.
Sticking requirement: the lower edge of barcode
should be within 15 mm ～20mm away from the
test tube bottom to make sure right reading
barcode, and make sure the barcode is aligned
with sample position gap when putting the test
rack.
Reaction system Reaction cup

Discrete
mode
Reaction cup
6mm
optical path
Reaction cup
6 sets, 20 for each，total 120.
quantity
15 minutes at most, 3、4、5、10 and 15 minutes

Reaction time
available）
Reaction liquid 150～450ul

volume
Light source 20W/12V Long-life quartz halogen
Absorbance
0～3.3ABS
range
QC QC interval, monthly QC
Automatical Automatically rinsing reaction cup, reagent probe,

rinsing sample probe, stirring rod.
18
Stirring
Separately stirring after adding reagent
system
TCP/IP network interface, standard RS-232 and

interface
USB 2.0 interface.
Stylus printer, supporting the user-defined mode for

Printer
Data system report sheet
Connecting
LIS/HIS LIS/HIS system available
system
weight Approximate 300Kg
Dimensions 1060 mm×790 mm×1150mm(length×width×height)

Instrument system
Power（VA） 2000VA
Water
25L/h
consumption
Power supply 220V/230V，50Hz/60Hz，2000VA
Using System storage temperature:0℃ ～ 40℃ ，

environment volatility <±2℃/H；storage humidity:30%RH～
Installation
requirement 80%RH ， non-condensing ； at working,
temperature:15℃ ～ 30℃ ，
volatility<±2℃/H ；
at working, relative humidity:35%RH ～
80%RH ， non-condensing ； not higher than
2000 meters above sea level。
3.1.2 Testing speed
Note：Due to the different specific conditions, sometimes equipment processing capacity will be
lower than 400 tests / hour.
Test conditions Degree of reduced ability to process (estimated)
Retest after sample

133tests/h（all tests after redilution ）
prediluted
200 tests/h at least（reaction cup、sample probe）

Use avoiding cross
contamination function
200～400tests/h （reagent probe）
R1 and R4 items or R2 and

R3 items are used 200tests/h at least
simultaneously in testing.
19
3.2.2 Test Flow Instruction
3.2.2.1 Periodic movement sequence of sample probe
a. Internal and external wall cleaning

b. Rotate to above sample disk and assimilate 2ul air.
c. Descend till the sample probe point into liquid level more than 2mm
d. Assimilate quantitive volume + push back redundant sample
e. Rise from sample test tube and rotate to above reaction disk
f. Descend into reaction cup to discharge quantitive volume of sample
g.Rise and rotate to above rinsing bath
→ (next periodic movement sequence).
3.2.2.2 R1、R4 reagent probe periodic movement sequence :
a. Internal and external walls rinsing
b. Rotate to above reagent disk and assimilate 5 ul air
c. Descend till the reagent probe point into liquid level more than 2mm
d. Assimilate quantitive volume + redundant volume of R1 and R4
e. Rise from sample test tube and rotate to above reaction disk
f. Discharge quantitive volume of R1 and R4
g. Rotate to above rinsing bath
→ (next periodic movement sequence)。
3.2.2.3 R2、R3 reagent probe periodic movement sequence:
The same to R1
3.2.2.4 Stirring rod periodic movement sequence：
a. Rotate to above reaction disk
b．Descend into reaction cup
c．Mix reaction liquid
d．Rise from reaction cup and rotate to rinsing bath
e．Descend into rinsing bath

f．Stirring rod rinsing
g．Rise from rinsing bath
20
3.2.2.5 Movement and time sequence of reaction disk
A track includes total 120 reaction cups in reaction disk, and rotates in a fixed way when
testing. The reaction cup always rotates and stops 3 times counterclockwise, total 22＋37
＋2=61 （rotation and stop sequence 22－37－2－）patches, in every working period, 9
seconds elapsed. 122 patches are passed in two working periods within 18 seconds.
3-2 Position of reaction disk and probe
Rinsing mechanism Reset point Position No. of reaction cuvette.
Photoelectric
detection
Sop and wipe 62,R1 stirring p
63,R4 stirring po
Sample probe
R1, R4 Probe
60 R1 Pipetting
61 R4 Pipetting
Reference position
number
Reagent probe 2.3 Stirring rod
Outer circle figure: No. of reaction；inner circle figure: No. of mechanism position;
Sample position: No. 1 position; reagent 1,4 probes: No.60,61 position; stirring at
NO.62,63 position; reagent 2,3 probes: No. 31 position, stirring: No. 33 position; reaction
cup rinsing mechanism: No. 101、103、105、107、109、117、11 position.
Reaction cup stops at No. 101 position after reaction disk resetting. The sequence of
reaction cup rinsing and sampling:
1→62→3→64→5→66→7→68→ …… →59→120→（9 minutes，60times）
61→2→63→4→65→6→67→8→ …… →119→60（9 minutes，60times）
21
3.2.3 Reaction Cup Rinsing Movement Sequence
3-3 Reaction cup rinsing probe position
Rotati
Rotationa 测4 4 次杯空白
times （1 次停止，
cell blank
l l
Rotational direction 次通过）
measurement.
direction
1 stop, 3 pass.
f
Above figure shows that seven steps are needed when rinsing reaction cuvette. (four times
cell blank test is added) , therefore, to finish rinsing one reaction cuvette, 11 steps are
needed：
3.2.4 Optical measurement movement sequence
Photometry in the entire process is adopted. In 15-minute reaction time, the continuous
determination of the absorbance of reaction solution is carried out. Reaction disk rotates 1
plus 2 pitches, about 18 seconds, absorbance values are measured out when the 120
reaction cups passing optical axis of the photometer one by one.
Each reaction Cup in 3-minute reaction time was measured 10 times (10 photometric
points), 4-minute reaction time was measured 13 times (13 photometric points), 5-minute
reaction time was measured 16 times (16 photometric points), 10-minute reaction time was
measured 33 times (33 photometric points), 15-minute reaction time was measured 49
times (49 photometric points).
22
3-4 Optical system
Figure 1-13 Photometer
Light starting from the light source was focused by the lens, and passed the reaction cup
first, and then was disparted by concave grating. After spectrophotometry, each
wavelength were received by 12 fixed photoelectric sensor simultaneously, and were
amplified 12 amplifier, after Log transformation to derive the rate of change of absorbance
or absorbance. When dual-wavelength testing is used, the concentration value is
calculated by the difference of the main and sub-wavelength absorbance or that of
absorbance change rate, and therefore dual-wavelength testing can not only compensate
the blood lipid, hemolytic, jaundice sample test, but also compensate on the result
impacted by voltage changes, so that measurement is more accurate, more stable.
23
Chapter 4. Module Introduction
4.3 Sample / reagent probe unit
4.3.1 Function introduction
Sample/reagent probe unit includes sample probe, No. 1 sample probe(R1) and No.2
sample probe (R2), which are called 3-probe component.
Sample probe can realize the assimilation from the sample test tube and sampling into
reaction cup. No. 1 sample probe(R1) and No.2 sample probe (R2) can realize the
assimilation from the reagent bottle and adding reagent into reaction cup.
In addition, main function of 3-probe component: liquid level detecting and bump-proof in
movement process, sample probe block detecting function.
Other subsidiary function includes mechanical limit, power-down self-locking function.
3-probe component working position：
1. sample probe component ： rinsing bath→sample disk assimilating

position→reaction disk/ISE sampling position；
2. reagent probe component ： rinsing bath→reagent disk assimilating

position→reaction disk/ISE adding position
Probe drive mechanism plays a key role of reagents and samples adding. The way of
probe are only up-down and circular moving, so two step motors are necessary to drive.
24
4.3.2 Composition and configuration
Figure 4-1 probe components configuration
Probe rotating arm

Probe rotating gear
belt
Probe rotating step

motor
Probe up-down
weight block
Probe up-down light
sensor block tablet
Probe up-down
guide slider
Probe up-down step
motor
Probe up-down
gear belt
Reagent 1, reagent 2 and sample probe drive mechanism in addition to different probe
turning angles, namely the corner mechanical limit block tablets different, the other
structures are identical.
4-2 Probe body and rotating body
Rotating fixed light Rotating motor

sensor position mounting hole
Guide
movements
long hole
Up-down
motor
mounting
Lower rotating axle hole
center
25
Probe rotating gear
Probe rotating
knight head
lower probe rotating

axle
Rotating probe drive ratio 12:34, using 0.9° stepper motor and 8 segment controller.
Control accuracy can achieve 0.0398°.
Up-down main gear driver diameter is 19.1mm, the 60mm for the perimeter, also using 0.9°
stepper motor and 8 segment controller. Up-down control accuracy can achieve
0.01875mm.
4.4 Rotating mechanism unit
4.4.1 Function Introduction
The main function of rotating mechanism is bearing of the sample warehouse, reagent
warehouse and reaction disk, and drive it to rotate, so that sample, reagent carried in
reaction cup rotate to the designated location to finish sampling, mixing and other work.
Reagent disk 1, reagent disk 2, the sample disk and reaction disk mechanisms are classified
as turntable mechanism. Meeting the requirements of functionality and performance
simultaneously, in order to improve the craftwork of product, the four disks are designed as the
same frame structure.
4.4.2 Rotating mechanism configuration
1, the sample turntable: The sample storehouse, disk rotating bracket, step motor-driven
components
2, reagent turntable: The reagents storehouse, disk rotating bracket, step motor-driven
components
3, the reaction disk turntable: reaction plate, the reaction cup, incubation bath, disk rotating
bracket, step motor-driven components
26
Figure 4-3 Disk rotating bracket
Stirring mechanism
rotating motor
Mandrel Storehouse fixing

t
S/Driving seat
Zero light sensor
Reaction disk motor

Storehouse cover light sensor seat
Code disk light sensor The foot of bracket
Reagent disc 1 and disc 2 are same the structure , and the sample disk drive structure and
the drive transmission ratio are the same with reagent disc.
Figure 4-4 sample/reagent disk motor driver configuration
Driven gear
Drive gear belt
Code disk and light Driving step motor

sensor
System transmission ratio is 10:1.Because of using 0.9 °step motor with 8 segment driver
circuit, the wheel rotation accuracy can achieve 0.01125 °.
27
Figure 4-5 Sample/reagent disk assembly configuration
Leader of disk cover touch

switch
Connecting loop of Connecting loop reagent box

disk cover rack or sample tube disk rack
Figure 4-6 sample/reagent disk transmission code disk configuration
The only difference of drive structure of reagent and sample disk is the code disk.
Disk cover switch

light sensor
45 teeth of reagent
code disk
Original point block

and light sensor
Sample code disk

50 teeth of outer
track ,inner track
40 teeth
Original point light

sensor
28
4.5 Cooling mechanism
4.5.1 Function Introduction
R1 and R2 with two refrigerated reagent disk, adopting semiconductor refrigeration, the
temperature maintains at 6 degrees -10 degrees, 45 reagent positions for each reagent
disk respectively(the 45th fixed position for placing phosphor-free CS-anti-bacterial
cleaning liquid in each disk)
4.5.2 cooling system configuration and installation
Figure 4-7 reagent cooling storehouse reagent box rack assembly configuration
Equipped with
temperature-keeping reagent
cooling storehouse
Cooling water circulation

outlet
Reagent box
Barcode reader
scanning window
29
Figure 4-8 reaction disk assembly configuration
Gear drive is adopted to reaction disk due to the relatively high positioning precision.
Fixing pin
Colorimetric cup
rotating rack
Driving gear
Driven gear
motor vibrator
Figure 4-9 reaction cup installation disk assembly configuration
120 colorimetric cup

120 teeth of code disk in
colorimetric cup position
Locking screw of
colorimetric cup rack
30
Figure 4-10 Incubation bath components configuration
R1 stirring rod
rinsing bath
Liquid level sensor R1 probe rinsing

rack bath
Optical
window
Spilling outlet of
Circular constant incubation bath
temperature water
outlet Circular constant
temperature water
inlet
Figure 4-11 reaction disk with incubation bath components assembly configuration
Phosphor-free
Incubation bath detergent inlet
Figure 4-12 reaction disk and optical system components assembly configuration
Optical system
31
4.6 Stirring unit
4-6-1 function introduction
Stir and mix reagent after adding it
Reagent 1, reagent 2 agencies are identical in addition to the mixing angle of rotation,
namely the code disk is different.
4-6-2 Stirring components configuration and installation
Figure 4-13 Stirring components configuration
Mechanism rotation
mechanical limit
Rotation code disk

and light sensor
Stirring mechanism
up-down motor
Stirring mechanism
up-down slider
Rotating mechanism of stirring and rotating arm adopts the direct drive way of
step motor output axle, using 8 segment drive circuit of 0.9 ° motor, and the control
accuracy can achieve 0.1125 °. The largest angle is limited by the open angle of
rotatation code disk mechanical limit. And positioning is determined separately by
the left and right light sensors.
32
Figure 4-14 stirring up-down driver configuration
Stirring mechanism
up-down light Stirring mechanism
sensor up-down motor
Up-down slider axle
Curve axle and curve handle drive is adopted by stirring up-down mechanism .
Figure 4-15 stirring up-down slider drive configuration
Up-down linear
sliding axle
Up-down
guide axle
Up-down
slider
Because of mixing body movements are used by way of the crankshaft crank,
so the movements of the positioning accuracy at different locations different.
Landing and taking-off between the location of the speed of the highest, lowest
accuracy, and precision at both ends of the highest, the lowest speed.
Electrical axis from the axis of the slider bearings the size of 16.5mm, crank in
horizontal position, the landing position accuracy of 0.032mm.
Due to curve axle and curve handle drive is adopted by stirring up-down
mechanism, the up-down positioning precision are different at different position.
The speed is the highest and precision is lowest at the middle, however the
precision is highest and speed is lowest at the two ends.
The size of Motor axis from the axis of the slider bearings is 16.5mm, when
curve handle locates horizontally; the up-down position precision is 0.032mm.
33
Figure 4-16 stirring mechanism up and down positions
Up-down
slider
Up-down
slider
4.7 Colorimetric cup rinsing mechanism
4.8 Figure 4-17 Colorimetric cup rinsing mechanism
Rinsing probe Rinsing probe of

rack of colorimetric cup
colorimetric cup
Up-down
step motor Up-down slider
Only one-dimensional movement available to colorimetric cup rinsing

mechanism, and driving mechanism adopts the up-down driving mechanism of
stirring rod.
34
4.9 Rack
Rack includes the main rack, electrical, gas liquid valve and water tanks and
other racks.
Figure 4-18 Main rack configuration
Supporting board of upper Standing Main worktable Cable leading

cover supporting board board groove
Isolating
standing
board
Adjusting foot
All-directional
caster
Figure 4-19 electrical rack configuration
Water-proof cover Electrical control Cooling controller

board box
Isolated
transformer
Power switch
rack
Electric rack is mainly consisted of control panel box, power rack and
waterproof board and such parts.
35
Figure 4-20 gas、 liquid valve rack configuration
Gear pump pressure gauge

Waste liquid separating tank
Water inlet
Waste liquid outlet

Vacuum tank
Vacuum pressure switch
Vacuum pump
Figure 4-21 Valve rack, gear pump rack and the main rack assembly.
Gas liquid valve main rack
Gear pump rack
36
figure 4-22 Water-in tank unit configuration
Heating water tank
Pressure gauge
Vacuum exhausting
tank
Magnetic pump
4.10 Optical system
CS-400 adopts advanced flat field grating photometer. Concave holographic grating
photometer is today's domestic and foreign advanced optical system with simple
structure, high optical efficiency, and signal to noise ratio and measurement speed
of machine have greatly been improved, compact photometer dimension, stable
performance, long life, and such highlighting advantage, achieving the requirements
such as multi-wavelength, multi-item simultaneously testing at high speed,
multi-wavelength (12) simultaneously collecting signals, a relatively large aperture,
good imaging quality and post-spectrophotometry.
Figure 4-23 Optical assembly
Water-cooled
light source
Bunching lens set Diaphragm
Photoelectirc
cable array Flat field grating
Log applifier
37
Chapter 5. Instrument liquid and Gas Line.
5.1 Main function of liquid line
The CS400 liquid line system can be divided into five parts: water inlet tank,
refrigeration, 37 degrees centigrade temperature, colorimetric cup cleaning, the
inner and outer arms and stirring rod cleaning.
1. CS400 liquid line system includes sampling subsystem and cleaning

subsystem.
2. Sampling subsystem uses three probes plus two stirring rods and three
injectors. The sampling injector uses 100uL, and the two reagent
injectors use 500uL.
3. The inner and outer wall cleaning of three probes and two stirring rods
uses barotropic driving.
4. Cleaning bath: five cleaning bathes plus waste liquor in the reagent
storehouse, together with the flooding waste liquor in the reaction disk
are seven kinds of routine waste liquor.
5. The reaction cup cleaning uses the way named 7-stop 11-step.
6. Water supply: uses the special outboard water-supply equipment and
the special water-supplying machine.
7. Waste liquor: use plastic hollow main pipe whose inside diameter is 20
to 30 mm, and the wall thickness is 3 to 5 mm. The installation of the
main waste liquor pipe is height limited, and it requires the height is
helpful to the waste liquor entering the low concentration liquid buffer
vessel by its self-weight. As to the high concentration waste liquid, it is
providing liquid level sensor interface and outboard high concentration
waste liquid barrel.
8. Source of power: the power of cleaning comes from the magnetic
pump..
9. The cleaning of reaction cup should use two kinds of detergent..
10. The vacuum degree for vacuum pump assimilating is between -28k and
-35kPa.
11. The inner and outer wall cleaning of three probes uses independent
solenoid valves while the two stirring rods use one solenoid valve
together.
38
5.2 Liquid Line Principle
Figure 5-1 CS－400 liquid line sketch map
39
Figure 5-2 liquid line of water inlet tank
40
1, when the low water level float detects out the signal, open the inlet valves
SV8, and water tank begins to be infused water until the high water level float
detects out signal, then turn off SV8 to stop the water. Influent flow is as follows:
Alarm of low water influent water Alarm of high water level Stop influent
level check check water
2, when the low water level float did not detect out signal, the water tank heater
began to work, and temperature control started. Magnetic pump began to work
simultaneously.
3, Output water pressure of water tank is controlled by the magnetic pump and fixed
damper regulator. Magnetic pump head is 8 / 11 m. Control water pressure is around
0.8kgf/cm2.
4, The outlet of tank water leads directly to the cleaning and incubation bath; the
other output water through gear pump supercharger to the pressure 1kgf/cm2 to 3
line probe mechanisms to rinse inner wall and pipeline, with exhausting device of
probe liquid line.
5, SV13 valve remains closed status so as not to affect water pressure during the
normal use of instrument. Only in the implementation of the maintenance of water
tanks, open SV13 valve, at the same time, time switch of SV8 inlet valve to empty
impurities in a water tank.
6, when abnormality occurs to the high and low water level floats, possibly water
tank remains the status of inputting water. When the water tank is full of water, SV8
not shut down, tank outflow water spills out from the overflow pipe into the waste
liquid barrel through waste liquid pipe.
41
Figure 5-3 Semiconductor cooling refrigeration liquid line
1.In the system, two reagent disks as well as standard and QC sample need to be
cooled in the environment from four to ten degrees centigrade. Water is used to
exchange temperature and transfer heat.
2. the system also adopts the semiconductor refrigeration mode which is better for
environmental protection and convenient for maintenance than the traditional.
3.each refrigeration unit is made of four groups of same semiconductor refrigeration

module string by parallel connection. Each module is constituted by one piece of
Peltier (semiconductor refrigeration), one water cooling block and one radiator.
4.the refrigeration power of each module is about 80W, the total 320W. Radiation
adopts pyrotub wind-cooled heat pipe to radiate. The single power is 150W.
5.the refrigeration warehouse shell with two reagent disks adopts stainless steel
structure, and the cold water flows into it from the bottom and flows out from the top
42
through circumvolution circulation, thus the warehouse interlayer is full of cold water
and its temperature is even.
6.the refrigeration volume of sample disk is small and the refrigeration area is
formed by aluminum cylinder, so the aim of refrigeration can be achieved by
circulating stainless steel tube. The whole circulation process is finished by magnetic
pump with a head 2.7m.
7. sluice water tank can check temperature and water level. The scope of
temperature is from 5 to 15 degrees centigrade above zero.
8.the refrigeration water tank is complete closed.
When adding water, it should be added from the inlet tube at the bottom by filler.
About 1.5 litter is added into water tank or flowing-out water from the top of water
tank observed (the two water-changing nozzle should be above the water tank,
water-changing tube is mainly used to exhaust). Here, turn on the main power
supply and the refrigeration system starts to work. Then the water level will drop,
water should be added continuously until the exhausting tube reflows out water.
When working stably, there is no change with the water level of exhausting tube, and
the two water-changing tube plugs should be installed. If there is no leakage, the
system water changing has been finished.
When spouting the water, please turn off the main power supply, pull out the two
water-changing tube plugs and contain water with container. The total water volume
is approximately four litters.
Use the fresh water to do the refrigeration circulation, check it annually.
43
Figure 5-4 constant temperature system liquid line
This system offers precise constant 37 degrees water to the incubation bath of reaction disk,
and cools the high temperature light source simultaneously. This system consists of magnetic
pumps, inlet valves, release valves, liquid level detector and temperature controller which
consists of the heater temperature
1, Open the inlet valve SV10 and turn off outlet valves SV16 to infuse water into
incubation bath, simultaneously with reagent R1 and R2 probes adding
phosphor-free anti-bacterial rinsing liquid to the incubation bath, liquid level detector
determining whether to stop water.
2, Turn off outlet valve and inlet valve, and start the water circulation magnetic
pump and temperature controller. In order to improve the adjusting performance of
the PID temperature controller in high temperature environment, the system is
added the cooling device through the water tank to get a small amount of
temperature cooled.
3, Turn off magnetic pump and temperature controller, open the drain valve SV16,
time to turn off the drain valve when the incubation bath is draining.
44
Figure 5-5 probe internal and external walls rinsing and sample probe block check liquid line.
1, Opening the valves SV1 and SV41 simultaneously can get the inner wall of
sample probe rinsed; open valve SV2 or SV3 to carry out reagent probe R1 or R2
internal wall cleaning. Probe position should be at the top of the corresponding
cleaning trough when cleaning so that waste liquid can get out of the instrument.
2、Open the valve SV4、SV5、SV9 or SV6 to rinse the external walls of sample,
reagent R1, R2 or 2 stirring rods. Each stirring rod has one corresponding valve
respectively. Fixing pressure adjusting piston is adopted to every external wall
rinsing pipeline to avoid rinsing water spilling out of rinsing bath.
3, valve SV1 pressure testing and the valve SV41 constitutes series structure, not
only completing the sample probe cleaning of the inner wall, but also completing the
block detection of probe, which does not affect the accuracy of assimilating and
discharging liquid volume of a small amount of sample.
4. The block detection must be implemented when probe is cleaned. Turn off valve
SV1 after the cleaning, Detect pressure, and probe is completely blocked or partially
blocked if the pressure value change is the same or smaller than the range value.
And previous added sample should be deserted and alarm is issued. Turn off SV1
and SV41 when probe assimilates and discharges sample.
45
Figure 5-6 Colorimetric cup rinsing liquid line
In order to achieve the cleaning effectively, colorimetric cup rinsing adopts warm
water. In order to improve cleaning speed, colorimetric cup adopts hydraulic valve
switch and vacuum liquid exhaust.
1, warm water provided by heating tank of inlet water is about 35 ℃. Water

pressure is produced by magnetic pump from the water tank and adjusted by
voltage regulator to the stable pressure, about 0.6kgf/cm2.
2, vacuum pump, vacuum tank and pressure detector composes vacuum source
with pressure value about -0.8kgf/cm2. Vacuum tank has some fluid, gas
separating role in order to guarantee the safety of vacuum pump besides having
stable vacuum pressure.
46
3, There is a device on the vacuum tank to eliminate air bubbles with liquid, gas
separating bottle.
4, vacuum liquid discharging, having concentrated and diluted liquid passages,

consists of the valves SV21, SV30, SV31 and concentrated, diluted liquid
separating bottles. Concentrated liquid means reaction liquid, including relatively
concentrated sample and reagent of patients, requiring separate collection.
5, when the colorimetric cup cleaning mechanism descends, turn off valves SV30
and SV31 first, and then open the valve SV21. It tarts assimilating sample under the
vacuum pressure, and the liquid of colorimetric cup will be discharged after a short
period of time when the rinsing mechanism arrives at the bottom of Colorimetric
Cup.
6, cleaning mechanism begins to add cleaning solution and ionized water, turn off
valve SV21 after the addition is completed, and then open valves SV30 and SV31.
At this time, the waste liquid discharged into isolating bottle outflows by its gravity.
7, cleaning liquid and ionized water adding is completed by the five valves SV42,
SV43, SV14, SV15 and SV11 which are timed. Because the vacuum liquid
discharging begins to work simultaneously when adding liquid to discharge
redundant liquid, the liquid will not spill outside colorimetric cup.
8, SV12 and SV14 valves are responsible for adding cleaning liquid. Before adding
or after the previous adding, open the valve SV12, but SV14 valve is at COM and
NO conduction status (power off status). Because there is a one-way valve in the
3-way top pipeline, cleaning fluid flows into the middle pipeline of the SV12 and
SV14 valves, and time valve SV12, cleaning liquid will remain in pipeline. Open
SV14 valve when adding, cleaning liquid will be added into colorimetric cup through
single way valve under the pressure. About 70ul cleaning liquid is consumed every
time.
47
Figure 5-7 ISE liquid line
48
49
Chapter 6 Instrument Hardware Circuit
ISE A/D collection
module
ISE valve sets
ISE
ISE pump sets
A/D convertion and
collection
module
A/D
Photoelectricity conversion and
amplification
（including the control of AC

and pressure temperature
SOPC main control board
R2 adding mechanism
module
R2 reagent disk
R2
PC
monitoring）
R1 adding mechanism
module
R1 reagent disk
R1
Hardware configuration
Figure 6-1 Hardware main frame
Sample
module
Sampling mechanism
Sample disk
Reation
module
stirring mechanism
Rinsing mechanism
Reaction disk
6.1
6.2 Security Note:
At working, touching hardware panel with hand or any other objects is forbidden.
In order to dismount panels, operation is only allowed when cut off power (220V, AC).
6.3 Electrical panel list
Electrical panel No. and function list
Figure 6-1 CS－400Circuit board list
PCB Name Description Electrical

panel No.
1、carrying on the communications between upper
and lower machine, and that with cooling board.
Main controller 2、Solenoid valve control：SV8、SV10、SV13、SV16、
SV41
board
3、Monitoring of high and low temperature water tanks
4 、 Monitoring of incubation bath and vacuum tank

positions,
5、AD board data processing
6、Solid-state relay board control
1, communications with the main control board 2, two

stirring mechanisms control
Reaction disk 3, the reaction disk rotation mechanism control
4, rinsing mechanism control

Circuit board
5、Solenoid valve：SV6、SV11、SV12、SV14、SV15、
SV21、SV30、SV31、SV42、SV43
6、Alkaline cleaning liquid level monitoring
1, communication with the main control board

2, reagent 1 probe mechanism control
Reagent 1 disk 3, reagent 1 disk control
4, solenoid valve control: SV2, SV5

Circuit board
50
5, buzzer control
Reagent 2 disk 1, communication with the main control board
2, reagent 2 probe mechanism control

Circuit board
3, reagent 2 disk control
Sample disk 1, communication with the main control board
2, sample probe mechanism control

Circuit board
3, sample disk control
5, the sample disk rotating lamp control
ISE 1, communication with the main control board
2, ISE pump motor control (internal standard, dilution,

Circuit board reference)
3, solenoid control: MAGNET1, MAGNET2
4, solenoid valve control: ISV1, ISV2, ISV3, ISV4,

ISV5, ISV6, ISV7, ISV8, ISV11, ISV12, ISV13
5, ISE preamp board data collection
1,200 W Heater Control
2,450 W Heater Control

Solid relay board
3, gear pump control
4, vacuum pump control
5, magnetic pump control
6, water circulation pump
7, halogen control
AD board 12 AD-wavelength data collection
Cooling board 1, semiconductor refrigeration control and temperature

display
51
2, semiconductor current monitoring and current value
display
3, fan control system
Circuit connecting 1, circuit board and the external circuit or device

connection
Board
2, part of the status indication (Indicator)
Level detecting 1, sample probe liquid level detection

board 2, reagent 1,2 liquid level detection
3, incubation bath liquid level detection
4, alkaline cleaning liquid level detection
5, vacuum liquid level detection
ISE Preamp K, Na, Cl electrode three-way preamp

board
Mother board 1, providing reaction disk board, sample disk board,

sample disk, reagent disk, reagent 1,2 disk boards,
main control board, ISE board with connection and
power supply.
2、communications among circuit panels
52
6.4 Instrument electrical principle wiring
Figure 6-4-1 Control wiring of communication system
53
Figure 6-4-2 Power switch wiring
Figure 6-4-3 Cooling board wiring
54
Figure 6-4-4 Main control board wiring
55
Figure 6-4-5 Solid relay panel wiring
Solid relay
board
12V switch
NES-35-12
Figure 6-4-6 Circuit panel control wiring of reagent R1 disk
56
Figure 6-4-7 Circuit panel control wiring of reagent R2 disk
57
Figure 6-4-8 Circuit panel control wiring of reaction disk
58
Figure 6-4-9 Circuit panel control wiring of sample disk
59
Figure 6-4-10 ISE panel control wiring
6.5 Circuit panel dismounting
When dismounting, pull out the connector on circuit panel first, and then loosen the set
screws to take out the circuit panel from circuit box.
60
6.6 Circuit function
6.6.1 Control configuration

CS-400 auto-chemistry analyzer structure consists of analysis part (host), operation part
(computer) and the result output part (printer).
Analysis part (host) mainly consists of the temperature control system, the reaction
system (including the ISE module), optical detection system, sample and reagent
processing system, mixing system, liquid line system and the reaction cup cleansing
mechanism.
Overall function of instrument control structure hardware system:
Achieve serial communication with the PC and the completion of command,

response and data transceipt；
control data acquisition of optical system;
control the movement and status signal collection of the movement

implementation mechanism;
as well as temperature control signal collection;
control ISE electrode data collection;
Control the cooling system.
6.6.2 Main control board function

Main function of main control board ：
communicate through the serial port with the PC to realize transmission

of data, instruction and warning information
communicate through the motherboard with the reaction disk board, the
sample disk board, reagent 1 disk board , reagent 2 disk board, ISE board, cooling
board, AD board to transmit data and instruction;
monitoring water tank water level and temperature
61
6.6.3 Reaction disk/ sample disk / reagent 1 disk / reagent 2 disk/ ISE board function
The main function of circuit boards of three disks are to receive the main control
board's instruction to complete the reaction disk, the sample disk, reagent 1 disk, reagent
2 disk, ISE circuit board work, the specific functions as follows:
Each CPU communicates with the main control board to receive instruction;
Each CPU outputs control order of each executive unit;
to receive the sensor signals and other status of implementation unit;
sent to the main control board If alarm occurs,
6.6.4 AD collecting panel function

AD collecting consists of two parts: AD data preamp board and AD data collecting
board
１、AD data preamp board：
Preamp board circuit realizes the photoelectric conversion function of discrete

photodiode array whose 12 pixels convert the multiwavelength homochromous light
signal after transmitting through reaction cup into electric signal, converted by
preamp circuit and sending the converted voltage signal to AD collecting board to be
processed.
２、AD data collecting board：
AD data collecting board circuit realizes signal adjustment of photoelectric signal

output by preamp board and AD collecting function. Photodiode array whose 12
pixels convert the multiwavelength homochromous light signal after transmitting
through reaction cup into electric signal, and the 12 photoelectric colorimetric
signal output by photoelectric check board is filtrated and amplified by AD collecting
board, sent to the input terminal of AD convertor by multi-choosing switch, and at
the same time, receive the control signal of main control board to sample one by one
from the processed 12 photoelectric signals, and send the AD value of reflecting
light intensity to the main control board to process. Besides, AD data collecting
board is responsible for the power supply of preamp board.
6.6.5 Cooling board function

Cooling system consists of three parts: semiconductor cooling model, radiator, and fan.
The main function of cooling board is to control semiconductor refrigeration module,

keeping the water of cold water tank at 6 degrees -10 degrees, to meet the needs of
reagent refrigerating warehouse refrigeration function, maintaining the temperature of
reagent refrigerated storehouse at the regulated range.
Main function:
control module semiconductor refrigeration

control cooling system fan
62
6.6.6 Power supply system
List of power supply used by circuit panel：
ＮＥＴ－５０Ｂ ±１２Ｖ
Series NE series small-size switching power supply
type NET-50B
Output voltage DC 5V,0.6-5A;12V，0.2-2.5A;-12V,0.1-0.7A
Output wattage 50W
Output set 3sets
Temperature -20～+60℃
range
Input voltage 85-264VAC/120-370VDC
size 129*98*38mm
Warranty period 2years
manufacturing Guangzhou
location
ＮＥＳ－１５－１２１２Ｖ
type NES-15-12
Output voltage DC 12V,0-1.3A
Output wattage 15W
Output set 1set
Temperature range -20～+60℃
Input voltage 85-264VAC(120-370VDC)
size 79*51*28mm
Warranty 2 years
period
manufacturing location Guangzhou
63
ＮＥＳ－１５－５５Ｖ
type NES-15-5
Output voltage DC 5V,0-3A
Output wattage 15W
Output set 1set
size 79*51*28mm
Warranty 2 years
period

ＳＰ－５００－２４２４Ｖ
Series PFC series switching power supply
type SP-500-24
Output voltage DC 24V,0～20A
Output wattage 500W
Output set 1set
Temperature range 0～+40℃
Input voltage 88～264VAC
size 170*120*93mm
Warranty 2 years
period
ＮＥＳ－３５－１２１２Ｖ
type NES-35-12
Output voltage DC 12V,0-3A
Output wattage 35W
Output set 1set
size 99*97*36mm
Warranty 2 years
period

64
Chapter 7 Maintenance and Overhaul
In order to ensure reliable system performance, excellent working status and span,
please conduct system operation and regular maintenance strictly in accordance
with the requirements in the repair manual. Learning maintenance and overhaul of
this chapter is also very important and in-depth study will enable the instrument to
achieve the best running status and exert the best performance.
Warning：
Do not carry out maintenance this chapter doesn’t mention.
Otherwise, it could lead to system damage and personal injury.
Do not touch any other parts except user self-operation and
maintenance which are clear recorded.
Unauthorized repair of the system may lead to system damage
and personal injury, and commitment term of the repair contract is
no longer valid.
Upon completion of maintenance work, make sure the system is
working normally.
Do not splash water, reagent and other liquid onto the system's
mechanical or electrical parts.
Biological contamination danger ：
In the process of maintenance work, be sure to wear gloves, put
on work clothes to prevent them from being infected and, if
necessary, wear protective glasses.
65
7.1Maintenace preparation
Tools, intensified cleaning liquid and alcohol maybe used in the process of working.
7.1.1 Tools
1. One set of hexagon wrench

2. Cruciform Screwdriver (large, medium and small)
3. Injection needle hose
4. Small tweezers
5. Clean gauze
7.1.2 Intensified cleaning liquid

1. Acid cleaning agent, 0.1mol / L hydrochloric acid
2. Alkaline cleaning agent, 0.5% (V / V) sodium hypochlorite
Warning：
Intensified acidic and alkaline cleaning liquid mixed generate
poisonous gas. Do not mix the intensified acidic and intensified
alkaline cleaning liquid.
Caution：
Following cleaning liquid designated by Dirui Co., Ltd.：
Intensified acidic cleaning liquid: 0.1mol / l hydrochloric acid;
Intensified alkaline cleaning liquid: 0.5% (V / V) sodium
hypochlorite.
Please use the intensified cleaning liquid designated by Dirui. If

outside of designated types of intensified cleaning liquid are used, it
may not be able to receive appropriate the results of the analysis.
Dirui recommends the use of alternating acidic and alkaline

cleaning liquid, for example, use intensified acidic cleaning fluid
after power on, then use of intensified alkaline cleaning liquid next
time after power on.
66
7.2 Daily maintenance item
7.2.1 Check injection pump
The purpose of checking the injection pump is check whether the leakage exists.
1, Make sure that Power of analysis part has been switched off.
2 open the front door and it is shown as Figure 6-1
If with ISE system, the left are the three ISE unit injection pumps, the middle are
injection pumps of sample, reagent 2, reagent 1 respectively.
Figure 6-1
1. To observe whether the injection pump is leaking,
If so, check the leakage causes, and check the pipeline and connector timely.
7.2.2 Check/rinse sample, reagent probe
1、In online status, click “Instrument resetting” in “Maintenance”, and instrument

executes resetting.
2、When cleaning ample and reagent probes, carefully observe whether the
outflow of sample probe internal wall is continuous, whether the direction of flow is
consistent with the sample probe and the outflow of external wall is continuous, ,
and whether water volume is normal.
If not normal, clean sample probe
If still not normal, check the corresponding liquid line channel; check whether
water supply of water tank and water pressure is normal.
67
7.2.3 Rinsing stirring rod
executes resetting.
2, when cleaning, carefully observe whether the stirring rod works normally, If not
normal, check the corresponding liquid line channel, check whether water
supply of water tank and water pressure is normal.
7.2.4 Rinse rinsing mechanism

executes resetting.
2、When rinsing, carefully observe rinsing probe working and whether probe
infusing is normal and assimilating is completely.
If infusing abnormal, check pressure value of water infusing pressure gauge
If assimilating abnormal, check pressure value of assimilating vacuum
7.2.5 Check waste connection and discharging
Biological contamination danger:
During waste water operation, please put on gloves, put

on work clothes and if necessary, wear protective glasses.
Check whether liquid waste disposal system is normal every day, and maintain
waste liquid pipe is not bent and discharges smoothly and high and low
concentration waste liquid are disposed properly (refer to local standards of dispose
waste liquid).
Caution:
Make sure liquid flow catheter was not bent and flows smoothly.
Otherwise, it may result in that poor waste water spills from the cover
panel of analysis part, even the serious damage of analysis part.
7.2.6 Rinse instrument surface

Rinse instrument surface daily to keep the clearness of instrument surface.
7.2.7 Check printer and printing paper
Check printer power supply indicator, preparation indicator and printing paper daily.
68
7.3 weekly maintenance items
7.3.1 Rinse sample probe/reagent 1/ reagent 2 probe
Warning:
Please be careful to avoid hands from being scratched
In operation, please put on gloves, work cloths, and put on protective

glasses for the best.
Do not dispose the gauze used to clean sample probe at your own will,
please follow the relevant provisions for proper disposal.
1 Make sure the analysis part power supply is switched off.。

2 Lift the rotating arms of sample and reagent probes by hands to the top
position, and rotate them to the top of sample or reagent storehouse for
convenient operation.
3 Caution:
When cleaning, do not touch directly the sample
surface to prevent probe scratch; avoid too much hand
force to prevent deformation of the sample probe.
69
Note:
Acidic and alkaline cleaning liquid can be used
alternatively, for instance, acidic cleaning liquid is used
at previous time maintenance, use alkaline cleaning
liquid at this time maintenance.
Wipe the external walls of sample and reagent probes lightly with cotton
stick moisturized with alcohol, especially the point of probe, until no
impurities left at all.
4 Wipe sample and reagent probes with the gauze dipped with deionized
water
5 After cleaning, lift the rotating arms of sample and reagent probes to the top
position, and rotate the rotating arm of sample probe to locate the sample
probe above the rinsing bath of sample and reagent probes.
Caution:
After cleaning the surface of a sample probe, please
make sure sample probe must be rotated to the top of
sample probe rinsing bath.
6 Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement “instrument
resetting", the system will automatically reset the sample and reagent
probes and rinse them with deionized water.
7.3.2 Rinse stirring rod

70
1 Make sure the analysis part power supply is switched off.。
2 Lift the stirring rod by hands to the its top position, and rotate its rotating
arm to the above a position for convenient operation.
3 Caution:
When cleaning, do not touch the sample surface directly to
prevent probe scratch; avoid too much hand force to prevent
deformation of the sample probe.
Note:
Acidic and alkaline cleaning liquid can be used alternatively,
for instance, acidic cleaning liquid is used at previous time
maintenance, use alkaline cleaning liquid at this time
maintenance.
Wipe the surface of stirring rod lightly with cotton stick moisturized with
alcohol, especially the point of probe, until no impurities left at all.
4 Wipe stirring rod with the gauze dipped with deionized water
5 After cleaning, lift the rotating arms stirring rod to the top position, and
rotate the rotating arm of stirring rod to locate the stirring rod to the top of
the rinsing bath of stirring rod.
71
7.3.3 Rinse sample/reagent barcode window
Caution:
Do not gaze scanning laser light, or it may cause eyes injury
1 Make sure the analysis part power is switched off.

2 Remove the reagent and sample disk covers, and then remove the sample
and reagent disks.
3 Wipe the scanning glass window lightly with gauze dipped with deionized
water.
4 Remount the sample and reagent disks and cover them.
5 Switch on the analysis part and wait 30 seconds, the system will reset
automatically.
7.3.4 Rinse sample storehouse
Warning:
Please be careful to avoid being scratched by sample probe.

2 Remove the sample disk（figure）
3 Rinse sample disk with water and wipe it with gauze
4 Wipe sample storehouse internally with clear gauze. If necessary, wipe it
with gauze dipped with a little pure water or disinfector.
5 Mount sample disk, tighten disk fixing screws clockwise and cover it.
72
7.3.5 Rinse reagent refrigeration storehouse
Warning:
Please be careful to avoid being scratched by
sample probe.
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.

2 Remove reagent disk cover and reagent disk
3 Rinse sample disk with water and wipe it with gauze
4 Wipe sample storehouse internally with clear gauze. If necessary, wipe it
with gauze dipped with a little pure water or disinfector.
5 Mount sample disk, tighten disk fixing screws clockwise and cover it.
7.3.6 Rinse reaction cup

The contamination of sample probe, reagent probe, stirring rod and reaction cup will
affect the accuracy of measurement. The reaction cup is required intensive cleaning.
1 Place 70 ml detergent (1mol of hydrochloric acid or 0.5% NaOH solution,

use the two types of solution alternatively, one week one solution) at the
45th detergent position of reagent disk.
2 Click “Maintenance” functional key to enter “System maintenance” menu,
and select “Rinse reaction cup” to execute.
73
7.4 Monthly maintenance item
6.4.1 Rinse sample, reagent 1, reagent 2 probe rinsing bath.
Warning:
sample probe.
2 Lift the rotating arm of sample probe by hands to the its top position, and
rotate its rotating arm to keep sample probe away from rinsing bath for
3 Clean the inside and appearance of sample probe rinsing bath with clean
cotton stick.
4 After cleaning, lift the rotating arm of sample probe to the top position, and
rotate the rotating arm of stirring rod to locate the sample probe to the top
of the rinsing bath of sample probe.
Caution：
After the work of sample probe surface rinsing, please
make sure to rotate the sample probe to the top of
sample probe rinsing bath.
74
7.4.2 Rinse stirring rod rinsing bath
Warning:
sample probe.

2 Lift the stirring arm to the top position by hand to one side of rinsing bath.
3 Wipe stirring rod with clean soft gauze.
7.5 Every 4－6 months maintenance item

7.5.1 Dust-proof disposal of cooling unit
2 Open right back cover board
3 Remove the three screws of cooling unit and remove the cooling unit.
4 Remove the dustproof from the cooling unit and rinse it with water and dry
it.
（figure）
5 Remount dustproof net cleaned already.
6 Mount back cover board
7.6 Every half a year maintenance item

7.6.1 Check light source lamp
Lamp light source of optical system will gradually be aged in use, and will cause an
increase in noise measurements. If the cup blank and light source intensity
attenuation is out of range or the working time of light source lamp accumulates over
2000 hours, the light source lamp should be checked.
75
Caution：
Please use consumables recommended by Dirui company, using other
consumables may cause system performance degradation.
Do not touch the light source lamp shell surface and lens in front of the
light source lamp by hand, because it may change the characteristics
of the light source. If you accidentally make noodle stained with filth,
absorbent cotton dipped by absolute alcohol can be used to clean it.
1 Turn off the system main power, so that the light source box and light
source lamp will be cooled for at least 15 minutes.
Warning:
High-temperature light source lamp and light box will
cause burn. Operation is carried out only after the light
source and light source lamp are cooled.
2 Loosen the fixing screws of rinsing mechanism; remove the rinsing

mechanism of reaction cup. Loosen the setscrews of reaction disk and
remove the reaction disk. Place the reaction cup at dry and clean position.
Loosen the two fixing wire connecting poles of halogen and remove
down-lead.
76
3 Loosen the two screws fixing light source seat to remove halogen lamp.
4 Mount a new halogen lamp according to the above opposite steps; pay
attention to tighten the screws. The cooling rubber hose in the lamp room
can not be twisted and down-lead can not be loosed or cocked.
5 Remount the reaction disk, the reaction cup and rinsing mechanism;
switch on the power supply of analysis part. After standby mode,
single-click “Next” in “System maintenance” window; infuse purified water
into reaction groove. After instrument standby mode, execute light quantity
check function. Check the back of halogen lamp if the light quantity
conforms to the requirement to start test.
7.6.2 Check all air filter net

After long-term use, the ventilation performance of air filter, which is not good,
needs to be checked.
7.6.3 Check and clean container and floater switches.

7.6.4 Check water liquid pipe
After long-term use, if any unsmooth discharging occurs, the waste liquid pipe of
designated specification can be used to check former waste liquid pipe.
7.7 Every 1 year maintenance item

7.7.1 Check water of cooling system
7.8 Irregular check item
7.8.1 Rinse sample probe and reagent probe
If the water flow is not normal when cleaning sample probe, sample probe and reagent
probe may have been blocked and cleaning is needed to sample probe.
77
Warning:
sample probe.

2 Remove sample disk cover and then sample disk
3 Lift the rotating arm of sample probe by hands to its top position, and rotate
its rotating arm to keep sample probe away from rinsing bath for
4 Hold shell claw of probe rotating arm with fingers and lift to remove.
5 Loosen pipeline interface
78
6
warning：
Carefully place dismounted sample probe and prevent it
scratching human body and sample probe damage.
Note：
Take out sample probe from the rotating arm and be careful
to operate to avoid the damage of probe point caused by
touching rotating arm.
Note：
Sample probe is precisely processed to ensure the sample adding
precision. If the probe point is damaged or bent, checking sample
probe is a must, or no guarantee can be made for test precision. Please
refer to "Error! Reference source not found. Error! Reference source
not found." for specific check of sample probe.
7.8.2 Clean sample probe/reagent probe
Warning：
Do not dispose the gauze used to clean sample probe at
your own will, please follow the relevant provisions for
proper disposal.
1 Put a stainless steel wire through the sample probe point to clean the
impurity in the probe.
Caution：
probe is a must, or no guarantee can be made for test precision.
Please refer to "Error! Reference source not found. Error! Reference
source not found." for specific check of sample probe.
79
7.8.3 Install sample/reagent probe
Warning：

glasses for the best
Dismounting sequence is opposite to that of sample probe/reagent probe.
Caution：
probe is a must, or no guarantee can be made for test precision.
Please refer to "Error! Reference source not found. Error! Reference
source not found." for specific check of sample probe.
7.8.4 Rinse sample probe/reagent
When it is found that the water level of rinsing bath is too high when rinsing sample
probe because of no discharging available, which may be caused by the blocked
leaking hole. Cleaning sample rinsing bath is necessary.
Warning：

its rotating arm to keep sample probe away from rinsing bath for
3 Infuse about 1ml alkaline detergent of 0.5% (V / V) sodium hypochlorite or

84 disinfectant into rinsing bath for 10 minutes.
4 Switch on the power supply of analysis part
80
its rotating arm to keep sample probe above the rinsing bath of sample
probe.
Caution：
Please rotate the sample probe to the top of sample probe
rinsing bath after clean rinsing bath of sample probe.
6 Select and execute “Instrument resetting” after enter “Maintenance-routine

maintenance”, and the system will reset sample probe and sample probe
and rinsing bath will be rinsed with deionized water automatically. Observe
the outflow of sample probe rinsing bath.
7.8.5 Rinse stirring rod
If the stirring rod is damaged, please check stirring rod in accordance with following
steps strictly.
Warning：
Any touch is forbidden except at the knurling of it only by hand when
checking, and prevent any scratch on the flat part of stirring part.
Please deal with removed stirring rod properly.
Caution：

2 Prepare a new stirring rod and wipe the flat part of it with gauze or cotton
stick dipped with cleaning liquid, and then wipe it with gauze dipped with
deionized water.
3 Lift the rotating arm of stirring rod by hands to its top position, and rotate its
rotating arm for convenient operation.
81
4
Caution：
When pulling out stirring rod, make sure the direction of
force is vertical to that of axis of rotating arm. Lateral force
may damage the axis or stirring rod.
Remove stirring rod after loosen the two fixing screws.
5 Prepare a new stirring rod, and wipe the front of the stirring rod with gauze
dipped with 2% CS-antibacterial cleaning liquid.
6 When mounting new stirring rod, insert stirring rod till the bottom of motor
axis and tighten it with M2 screw.
Caution：
When inserting stirring rod, make sure the direction of force is
vertical to that of axis of rotating arm. Lateral force may
damage the axis or stirring rod.
Pushing the stirring rod completely
7 After stirring rod check, visually check whether stirring rod and its rotating
arm are vertical with each other.
If not vertical, return to step 5 and remount the stirring rod.
If vertical, continue to next.
8 Lift the rotating arm of stirring rod by hands to its top position, and rotate its
rotating arm to the top of its rinsing bath.
Caution：
Please make sure to rotate stirring rod to its rinsing bath top
after mount it.
resetting", the system will automatically reset the sample probe and rinse it
with deionized water. Observe the outflow of sample probe.
82
7.8.6 Check reaction cup
Warning：
Place each probe and pole into proper position for convenient.
Please deal with removed reaction cup properly which is broken.
Caution：
1 Switch off instrument

2 Put on protective gloves to remove fixing screws.
83
3 Rotate reaction disk by hand and remove the reaction cup sequently. Take out
reaction cup while rotating it.
4 Rinse the new reaction cup dipped in with water; rinse inside and outside of
reaction and no scratch is allowed.
5 Rotate reaction disk by hands, and mount new reaction cup on reaction disk
and check the six sets reaction cups simultaneously.
6 Mount reaction disk with the opposite steps and make sure the fixing screw of
reaction disk is tight.
7 Switch on the power of analysis part

Select and execute “cup blank test” after click “System maintenance”, and
observe execution result and reaction status.
84
Chapter 8 Assay Method
CS-400 adopting three analytical methods as follows:
point assay
two-point assay
rate assay
8.1 Analysis principle
The assay mode of Auto-Chemistry Analyzer is based on the Beer-Lambert law that the
material selective absorption light
The main principle is: When monochromatic light with specific wavelength passes
through the cuvette with sample, the monochromatic light absorbency and sample liquid
concentration are varies directly as the distance which is passed through sample liquid by
light:
I0
A = lg （1/T ）= lg （）= ε b c
It
A －Absorbency of the light when passing through liquid
T －Transmitted intensity and incident intensity ratio: transmittance It/I0；
I0 － Incident intensity
It － Transmitted intensity
ε － Molar absorption coefficient of solution（ml×mmol 1×cm 1）

－－
；
c － Mol concentration of the solution（mmol/ml）

；
b － Solution layer thickness（cm）

；
Solution layer thickness (b): Optical path, which is fixed by instrument. Molar absorption
coefficient (ε) is the correlation coefficient of the wavelength, solution and solution
85
temperature. Linear relationship is displayed between solution thickness and absorbency
when in stable temperature and single wavelength（ε value is given on the reagent bottle by
factory）
If the sample liquid adequate distribution, interaction between liquid and incidence
monochromatic light only happens during absorbing process. No fluorescence, disperse and
photochemical appear. No interaction between substances in the solution while absorbing
process. The absorbency possess conducts nature, and this condition conforms to the
Beer-Lambert law
8.2 Assay mode variety
Cell
Method Photometry point Formula Remark
blank
L– 0– 0– 0 B1 + B 2 + B 3 AL + AL −1
1-point
Assay 1＜L≤49 3 2
L– M– 0- 0 B1 + B 2 + B 3 ( AM + AM −1 ) − k ( AL + AL −1 )
2-point
assay 3 2
1＜L＜M≤49
AM + AM −1 AL + AL −1 测光点 L、
2-point B1 + B 2 + B 3 −
2 2 M 间的时
Rate Assay L– M– 0– 0 3
t 间（分）
1＜L＜M≤49
L– M – 0 - 0
Rate A B1 + B 2 + B 3
1＜L＜M≤49 △A（M-L）
Assay 3
L +2＜M
Fist half
1-point & L– 0 – 0 – 0 B1 + B 2 + B 3 AL + AL −1
Rate Assay 3 2
1 ＜ M ＜ N≤L ＜ P ＜
Q≤49
86
Second half
M–N–P–Q
B1 + B 2 + B 3
1 ＜ M ＜ N≤L ＜ P ＜ △（AQ- P）-k△（AN -M）
3
Q≤49
M+2＜N ，P+2＜Q
Fist half
B1 + B 2 + B 3 AL + AL −1
L– 0 – 0 – 0
3 2
1＜L≤M＜N≤49
3-point dual
item
Second half
( AN + AN −1 ) − k ( AM + AM −1 )
B1 + B 2 + B 3
M–N–0–0 2
3
1＜L≤M＜N≤49
Fist half
L– M – 0 - 0 B1 + B 2 + B 3
△A（M-L）
3≤L＜M＜N＜P≤49 3
L +2＜M
Rate B
Assay △ An 、 p （ diffe
(mode 1 ) Second half wavelength
N–P–0–0 B1 + B 2 + B 3 from the first Half）
Two conditions
3≤L＜M＜N＜P≤49 3
△An、p–k△AL、m（same
N+2＜P wavelength as the second
half）
Fist half
L– M – 0 – 0
Rate B B1 + B 2 + B 3
Assay 3≤L＜M＜N＜P＜Q △A（M-L）
(mode 2 ) 3
＜R≤49
L +2＜M
Second half
N – P –Q – R
B1 + B 2 + B 3
3≤L＜M＜N＜P＜Q △A(R-Q)–k△A(P-N)
3
＜R≤49
N+2＜P ，Q+2＜R
87
L,m,n,p,q,r : Photometric points
Rn ：Volume of nth reagent ,n=1 to 4

B1、B 2、B 3 ：Stopped cell blank
(B1,B2,B3 )/3 ：Passed cell blanks
Ax ：Absorbance at photometric point x
△A(m-L) ：Change in absorbance per minute between photometric

points L and M
k ：Liquid volume correction factor

a
S + ∑ Rj
j =1
k= b
S + ∑ Ri
i =1
S ：Sample volume
Rj 、Ri a: No. of reagents with correction (at Al measurement)
b: No. of reagents without correction(at Am measurement)
Note 1: The 5 th Photometric point won’t be Stirred after adding reagent 2. Stirring
when the reaction disk pauses after rotates one circle plus 2 pitches
Note 2: liquid in the reaction cuvette should be more than or equal to 150 ul, less
than or equal to 450ul.
Note 3: Do input 0 if the photometric point is not used.
8.3 Endpoint assay
Endpoint assay means the reaction takes a period time. Due to reaction balance constants
are big, all substrates (tested) are transformed into product when reaction reaches balance,
and no increase (decrease) of reaction solution absorbance will occur, and the degree of
absorbance increase (decrease) and the concentration of tested substance is directly
proportional. This assay is call “endpoint assay” or balance assay more accurately, which is
。
the ideal assay mode.
The endpoint assay is not sensitive to small changes (such as enzyme amount, pH,
temperature, etc.) as long as this change does not affect the balance in certain time.
88
figure 8-3 endpoint assay reaction curve
89
Example 1：TBIL－Total bilirubin reagent kit
wavelength Main 550nm，sub 660nm Absorbance 0～2A；

range
test mode endpoint assay Optical path 10mm
reagent 1：250uL sample 10uL
Single reagent A:B＝50:1 Mixing
reagent storage
temperature 37℃ incubation 10min
reaction 10min “0” 550nm，blank pipe
sensitivity 5mA equals to 1umol/L Linearity 300umol/L（18mg/dL）
range
calibrator 89.6umol/L，0.425A Unit 1umol/L＝0.0585mg/dL
conversion
Reference Adult；5.1～19umol/L（0.3～1.1mg/dL）
value Baby：20～200umol/L（1.2～12mg/dL）
Example 2、UA（uric acid）－Uric acid liquid reagent kit
wavelength Main 520nm （ 500,550 Absorbance 0～2A

optional） range
Test mode Endpoint assay Optical path 10mm
Reagent 1：200uL；2：50uL sample 4uL
Single 4 reagent 1and 1reagent 2 Mixing 2～8℃5days stable
reagent storage
temperature 37℃（30℃, 25℃） incubation 5min（6min , 8min ）
reaction 5min “0” 520nm，blank pipe
sensitivity 0.42mA equals to1umol/L Linearity 1.5mmol/L（25mg/dL）
range
calibration 0.72mmol/L，0.302A Unit 1mmol/L＝16.8mg/dL
conversion
Reference child：0.12～0.33mmol/L（2.0～5.5mg/dL）；
value Male：0.21～0.43mmol/L（3.5～7.2mg/dL）；
female：0.15～0.36mmol/L（2.5～6.0mg/dL）；
urine ：14.9～44.6mmol/L（250～750mg/dL）；
90
8.4 2-point assay
2-point assay (fixed time assay) is also called first class dynamics assay, which means the
reaction speed is in direct proportion to the simple power of substrate concentration in
specified time, namely v=k[S]. Due to the reduction of substrate, the whole reaction speed
is decreasing gradually, which reflects the decrease (increase) of absorbance and
decrease of speed. Because reaction time to reach balance is very long, , it can be
monitored at any time theoretically, but because of the complexity of serum ingredient and
much reaction, therefore, it takes a certain period of time to enter in stable reaction phase.
Figure 8-2 2-point assay reaction curve
Absorbance
Reaction limit level
Absorbance
Cell blank
Time
Time
2-point assay 2-point rate assay
8.5 Rate assay
Rate assay, also known as zero-class dynamics assay, refers to the reaction rate is directly
proportional to the zero power of substrate concentration, which has nothing to do with the
substrate concentration. Hence, the reactants can generate a certain product at constant
speed throughout the reaction process, resulting in even decrease or increase of
absorbance of measured solution at a wavelength, and the decrease or increase speed (△
A / min) is directly proportional to the activity or concentration of the tested substance
(catalytic material). Dynamics assay is also called as the continuous monitoring assay,
mainly used for the measurement of enzyme activity.
In fact, because substrate concentration can not be big enough, with the reaction
proceeding, the reaction is no longer zero class when substrate is consumed to a certain
extent, Therefore, zero-class dynamics assay is targeted at a specific period in terms of
time; Because reaction time to reach balance is very long, , it can be monitored at any time
theoretically, but because of the complexity of serum ingredient and much reaction,
therefore, it takes a certain period of time to enter in stable reaction phase. So all reagent
manufactures have strict requirements to the two periods
Dynamics assay is based on the changes between specified photometric points to obtain
the absorbance concentration or activity value.
Metering point in accordance with the input form, dynamics method can be divided into
single-band and dual-band dynamics assay.
91
Figure 8-3 Rate assay reaction curve
Absor
Cell blank
Time
Example 3：ALT/GPT － Alanine aminotransferase （IFCC）
wavelength Main 340nm Absorbance 0～2A

range
Test mode Rate assay Optical path 10mm
Reagent 1：200uL；2：50uL sample 15uL
Single 4 reagent 1and 1reagent 2 Mixing 2～8℃5days stable
reagent storage
temperature 37℃（30℃, 25℃） incubation 5min
reaction 60s delay，measure 60－ “0” 340nm，blank pipe
120s
sensitivity 0.30mA/min equals to Linearity 450U/L（7.5ukat/L）
1.0U/L range
calibration Unit 1U/L＝16.67×10-3ukat/L
conversion
Reference 37℃：Male：<40U/L（<0.67ukat/L）；Female：<31U/L（<0.52ukat/L）
value
Calculating method：
A/min * TV *1000
ALT（U/L）＝
6.22 * SV * P
TV＝The total reaction volume (mL）
SV＝sample volume（mL）
P＝optical path of colorimetric cup（cm）
6.22＝NADH position mmol extinction coefficient at 340nm （334nm：6.18，365nm：3.40）
92
8.6 Principle of electrolyte measurement
Principle
Internal standard solution is firstly added in diluting trough by instrument, and assimilates it
and discharges it into the Na, K, and Cl electrode solution line through the SIP injection
pump to measure its electrode potential which is relative to reference electrode potential.
At this time, SIP injection pump first assimilates reference electrode solution and
discharges it into the reference electrode solution line, and then switch to liquid line to
assimilate the internal standard. And redundant internal solution is assimilated by vacuum
nozzle to vacate diluting trough.
Diluent will be mixed after the sample probe assimilated sample and discharged it into
diluting trough. Diluted sample is as same as the internal standard solution and will be
taken out by SIP syringe pump and the internal standard solution is add into diluting trough
to rinse it. Used for cleaning, the internal standard solution will be pumped through the SIP
pump. After vacate diluting trough, assimilate the internal standard solution for the next
round of testing.
93
94
Assimilate internal solution to
rinse diluent bath
Measure sample concentration
Assimilate diluted sample from
diluent bath
Diluting sample
Infuse sample Discharge internal standard
solution
Measure and calibrate the concentration of
internal standard solution
Figure 8-4 ISE Work flow
Assimilate internal standard

solution from diluent bath
Infuse internal standard solution Assimilate reference liquid
into diluent bath
8.6.1 8.6.1 Principle of generating electrode potential
Electrode potential is obtained by Nernst's formula.
RT
E = E 0 + 2.303 × × l og(ai )
nF ………………………………………………………（1）
ai = f × Ci ……………………………………………………………………………（2）
E0 : The standard potential of the measured system
R: gas constant (8.314510 J × mol-1 × K-1)
T: absolute temperature (t ℃ +273.15) (K)
F: Faraday constant (9.6485309 × 104 C × mol-1)
: Ion (i) activity
f: activity coefficient
Ci: concentration
n: a given ion (i) the charge number (Cation is positive, anion is negative)
8.6.2 Test method
Working curve preparation, the internal standard solution concentration measurement,

concentration calculation, and result modification are explained as follow.
8.6.3.1 Working curve preparation
Measure low concentration slope of liquid (S1) and high concentration slope liquid (S2),
and determine slope value (sensitivity) of K, Na, Cl the electrode.
E ( H ) − E ( L)
SL =
C(H )
log
C ( L) …………………………………………………… (3)
8.6.3.2 The measurement of internal liquid concentration
E ( IS ) − E ( L )
C ( IS ) = C ( L) × 10 SL
…………………………………………… (4)
C (IS): the concentration of internal standard solution
E (IS): the potential of internal standard solution
95
8.6.3.3 concentration calculation
The calculation of routine sample, STAT sample, and concentration of quality control liquid
is based on the concentration of internal standard solution. Internal standard solution is
different with the different sample.
E ( S ) − E ( IS )
C ( S ) = C ( IS ) × 10 SL
………………………………………… (5)
C(S)：Sample concentration
E(S): Sample potential
8.6.3.4 result modification
Test calibrator (calibrator S3)of serum category after calibration to calculate its
concentration, and the difference between the tested concentration and input value is used
as compensation value to increase or decrease sample quantitative value.
C (VALUE ) = C (C ) − C ( X ) ………………………………………… (6)
C (VALUE): compensation value (compensation value)
C (C): Concentration input value of the serum calibrator
C (X): Concentration tested value of the serum calibrator
C ' ( S ) = IF {C ( S ) + C (VALUE )}
.. ... ... ... ... ... ... ... ... ... ... ... ... ... (7)
C '(S): modified sample concentration
IF: Instrument constant (usually 1.0)
8.6.3.5 Standard specification of electrolyte
Item specification
Sample 15ul
volume
Diluent volume 450ul
Processing 80sample/h（only measuring electrolyte）
ability
Measuring Na + 20 ~ 200mmol / L (when only serum )
range 10 ~ 400mmol / L (when measuring urine)
K + 1.0 ~ 15.0mmol / L (when only serum)
1 ~ 200mmol / L (when measuring urine)
Cl-20 ~ 200mmol / L (when only serum)
10 ~ 400mmol / L (when measuring urine)
Reagent Internal standard solution 1050ul / samples (only for

consumption continuous determination of
volume electrolyte )
Diluent 450 ul / sample
Reference Electrode Solution 130 ul / sample
Note:
Internal standard solution will be added if there is no electrolyte analysis for more than 10
minutes in order to activate the electrode. 96
Chapter 9 Malfunction Analysis
9.1 Stirring mechanism 1
Alarm Detailed
Description Solution
Code description
¾ In the CS-400 upper instrument software, enter

into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1：stirring mechanism stops
Solution:
1, Check stirring mechanism up-down movements,

mechanical repair is required if resistance is big.
R1stirring
mechanism 2, check whether both ends of connector of the motor
R1stirring fails to are connected well.
1-1 mechanism reach the
abnormal top of 3, check whether the conductivity of motor lead wire
rinsing bath is good.
side.
4, check the motor drive module of circuit board is
working normally
Malfunction 2：stirring mechanism can reach the top,

but can not check the zero position.
solution：Manually make stirring mechanism

repeatedly rise to peak, and observe ud_st1 adapter
indicator status.
1、sparkling of ud_st1 indicator means the wiring of

light sensor and adaptor is normal.
97
(1) Check whether the conductivity from adapter to
reaction disk circuit board is good and both ends of
connector are connected well.
(2) Check input part circuit of reaction disk circuit

board light sensor signal.
2、no sparkling of ud_st1 indicator, check ud_st1
（1）check lead
Check whether the conductivity from adapter J273 to

light sensor lead P273-P403）7、8、9 is good and both
ends of connector are good.
（2）check light sensor signal
check voltage between P273 plug pin 7,9, if the

voltage is not 5v, see (4); if voltage is 5v, check the
potential of 8-pin socket of the P273. The potential is
high when rose to peak, otherwise the potential is low.
If the potential is normal, check the adapter board; not
normal, check the light sensor
（3）check voltage between P285 plug pin 4，6, if the

voltage is not 5v, check adapter board; if voltage is 5v,
check conductivity from adapter board J285 to
reaction disk lead（P285-P075）is good and both ends
of connector are connected well.
Malfunction 3：Stirring mechanism can reach the zero

position.
solution：Mechanical repair
R1stirring
mechanism
R1 Stirring fails to
1-2 mechanism reach the ¾ R1stirring mechanism fails to reach the top，the
abnormal top of solution is the same as 1-1
reaction
side.

R1tirring into the "system maintenance" interface after
mechanism on-line, implement "mechanical movement
R1 Stirring check", and observe the stirring rod running
can leave
1-3 mechanism status
the top
abnormal Malfunction 1：stirring mechanism stops
when it
descents solution：
98
2, check whether both ends of connector of the motor

are connected well.
3, check whether the conductivity of motor lead wire

is good.

working normally
Malfunction 2：stirring mechanism can reach the top

but not leave
Solution ：the same to 1－1 Malfunction 2

status
Malfunction 1：：stirring mechanism stops
Solution：


R1stirring are connected well.
mechanism
fails to
is good.
R1 Stirring reach the
1-4 mechanism rinsing bath 4, check the motor drive module of circuit board is
abnormal when it working normally
moves to
rinsing 5、Check whether the it is at top position, check
bath. whether up-down zero light sensor works normally at
top position.
Malfunction 2： stirring mechanism can sway, but can

not reach rinsing bath.
Solution：
1、check whether the installation of left and right limit

light sensors is correct.
2、in boot-strap status, manually sway stirring

mechanism and observe the right_st1 indicator status
99
of right limit light sensor.
(1）sparkling of ud_st1 indicator means the light

sensor, lead and adaptor are normal.
1）check lead

reaction disk circuit board lead （P285-P075）is good
and both ends of connector are good.
2）Check input part circuit of reaction disk circuit board

light sensor signal.
(2）if no sparkling of right_st1,check right_st1
1）check lead
Check whether the conductivity from adapter J273

to light sensor lead （P285-P075）pin 4、5、6 is good
and both ends of connector are connected well.
2）check light sensor signal
check voltage between P273 plug pin 4，6, if the

voltage is not 5v, see（3）; if voltage is 5v, check the
potential of socket pin 5 of the P273. The potential is
low when sway to rinsing bath, otherwise the potential
is high. If the potential is normal, indicator remains,
check the adapter board; not normal, check the light
sensor
3）check voltage between P285 plug pin 4，6, if the

check conductivity from adapter board J285 to
100
status
Solution：


are connected well.

is good.

working normally
5、Check whether the it is at top position, check

R1stirring whether up-down zero light sensor works normally at
mechanism top position.
fails to
R1 Stirring reach the Malfunction 2： Stirring mechanism can realize its
1-5 mechanism reaction sway movement, but can not reach the reaction cup.
abnormal cup when it
moves to Solution：
reaction
cup. 1、check whether the installation of left and right limit
2、in boot-strap status,，manually sway stirring

mechanism，observe the left_st1 indicator status of
right limit light sensor.
（1）sparkling of left_st1 indicator means the light

1）check lead

reaction disk circuit board lead （P285-P075）is good
and both ends of connector are good.
2）Check input part circuit of reaction disk circuit

(2）if no sparkling of left_st1,check left_st1
1）Check whether the ud_st1 indicator has

malfunction
101
2）check lead
Check whether the conductivity from adapter

J273 to light sensor lead （P285-P075）pin 1、2、
3 is good and both ends of connector are
connected well.

voltage is not 5v, see（3）; if voltage is 5v, check
the potential of socket pin 2 of the P273. The
potential is low when sway to reaction cup,
otherwise the potential is high. If the potential is
normal, indicator remains, check the adapter
board; not normal, check the light sensor.
4）check voltage between P285 plug pin 4，6, if

the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J285 to reaction disk lead（P285-P075）
is good and both ends of connector are
connected well.
R1 Stirring When
1-6 mechanism resetting,
abnormal R1 Stirring
mechanism The solution is the same as 1-4
failed to
reach
rinsing
bath.
¾ Enter into system maintenance window and

When execute “Mechanical movement check”. Observe
resetting, the running status of stirring rod.
R1 Stirring Malfunction 1：stirring mechanism stops
R1 Stirring
mechanism
1-7 mechanism The solution is the same as 1-4
failed to
abnormal
leave
Malfunction 2： Stirring mechanism can realize its
rinsing
sway movement to rinsing bath, but can not leave it.
bath.
The solution is the same as 1-4
R1 Stirring
mechanism
R1 Stirring
failed to ¾ If stirring mechanism failed to reach the top when
1-8 mechanism
reach the rotating，the solution is the same as 1-1
abnormal
top when
rotating.
102
9.2 Stirring mechanism
Alarm Descriptio Detailed

Solution
Code n description

status
Solution:

2, check whether both ends of connector of the

motor are connected well.

is good.
¾ 4, check the motor drive module of circuit

R2 Stirring board is working normally
R2 Stirring
mechanism can Malfunction 2：stirring mechanism can reach the top,
mechanis
2-1 not reach the top but can not check the zero position.
m
at rinsing bath
abnormal solution：Manually make stirring mechanism
side.
repeatedly rise to peak, and observe ud_ st2 adapter
indicator status.
1、sparkling of ud_ st2 indicator means the wiring of

(1) Check whether the conductivity from adapter to

reaction disk circuit board is good and both ends of
(2) Check input part circuit of reaction disk

circuit board light sensor signal
2、no sparkling of ud_ st2indicator, check ud_ st2
（1）check lead

light sensor lead P272-P402）7、8、9 is good and
both ends of connector are good.
103
check voltage between P272 plug pin 7,9, if the

high when rose to peak, otherwise the potential is
low. If the potential is normal, check the adapter
board; not normal, check the light sensor

voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board J285 to
Malfunction 3：Stirring mechanism can reach the

zero position.
R2 Stirring
R2 Stirring
mechanism can
mechanis ¾ R2 stirring mechanism fails to reach the top，the
2-2 not reach the top
m
at reaction cup solution is the same as 2-1
abnormal
side.

status
solution：

R2 Stirring
R2 Stirring
mechanism can 2, check whether both ends of connector of the
mechanis
2-3 not leave the top motor are connected well.
m
when
abnormal
descending. 3, check whether the conductivity of motor lead wire
is good.

working normally
Malfunction 2：stirring mechanism can reach the top

but not leave
Solution ：the same to 2－1 Malfunction 2
104
status
Malfunction 1：：stirring mechanism stops
Solution：



is good.

working normally

whether up-down zero light sensor works normally at
top position.
R2 Stirring
R2 Stirring mechanism can
mechanis not reach the Malfunction 2： stirring mechanism can sway, but
2-4 can not reach rinsing bath.
m when moving to
abnormal rinsing bath
side. Solution：

2、in boot-strap status, manually sway stirring

mechanism and observe the right_ st2indicator
status of right limit light sensor.
(1）sparkling of ud_ st2 indicator means the light

1）check lead

reaction disk circuit board lead （P285-P075）is
good and both ends of connector are good.

(2）if no sparkling of right_ st2,check right_ st2
1）check lead
105
to light sensor lead（P272-P402）pin 4、5、6 is good
and both ends of connector are connected well.

low when sway to rinsing bath, otherwise the
potential is high. If the potential is normal, indicator
remains, check the adapter board; not normal, check
the light sensor

connected well.

status
Solution：

R2 Stirring 2, check whether both ends of connector of the
mechanism can motor are connected well.
R2 Stirring
not reach the
mechanis 3, check whether the conductivity of motor lead wire
2-5 reaction cup
m is good.
when moving to
abnormal
reaction cup
side. 4, check the motor drive module of circuit board is
working normally

whether up-down zero light sensor works normally at
top position.
Malfunction 2： Stirring mechanism can realize its
sway movement, but can not reach the reaction cup.
Solution：

106
2、in boot-strap status,，manually sway stirring
mechanism，observe the left_ st2indicator status of
right limit light sensor.
（1）sparkling of left_ st2 indicator means the light

1）check lead

reaction disk circuit board lead （P285-P075）is

(2）if no sparkling of left_ st2,check left_ st2
1）Check whether the ud_ st2 indicator has

malfunction
2）check lead
Check whether the conductivity from adapter

J273 to light sensor lead （P285-P075）pin 1、
2、3 is good and both ends of connector are
connected well.
check voltage between P272 plug pin 1，3, if

the voltage is not 5v, see（3）; if voltage is 5v,
check the potential of socket pin 2 of the P272.
The potential is low when sway to reaction cup,
otherwise the potential is high. If the potential
is normal, indicator remains, check the adapter
4）check voltage between P285 plug pin 4，6,

if the voltage is not 5v, check adapter board; if
connected well.
R2 Stirring
R2 Stirring
mechanism can
mechanis
2-6 not reach The solution is the same as 2-4
m
rinsing bath
abnormal
when resetting.
107
execute “Mechanical movement check”.
Observe the running status of stirring rod.
R2 Stirring Malfunction 1：stirring mechanism stops
R2 Stirring
mechanism can
mechanis The solution is the same as 2-4
2-7 not leave rinsing
m
bath when
abnormal Malfunction 2： Stirring mechanism can realize its
resetting.
sway movement to rinsing bath, but can not leave it.
The solution is the same as 2-4
R2 Stirring
R2 Stirring
mechanis ¾ If stirring mechanism failed to reach the top
2-8 mechanism fails
m when rotating，the solution is the same as 2-1
to rotate at top.
abnormal
108
9.3 Rinsing mechanism of reaction cup

Solution
Code n description

Solution:
1, Check stirring mechanism sway movements,



is good.

working normally
Malfunction 2：stirring mechanism can reach the

top, but can not check the zero position.
Rinsing
mechanis Rinsing solution：Manually make stirring mechanism
m of mechanism of repeatedly rise to peak, and observe ud_wash
3-1 adapter indicator status.
reaction reaction cup fails
cup to reach the top
abnormal 1、sparkling of ud_wash indicator means the wiring
of light sensor and adaptor is normal.
(1) check lead

to light sensor lead（P285-P075） is good and both
ends of connector are connected well.
(2) Check input part circuit of reaction disk circuit

(2）if no sparkling of left_ wash, check left_ wash
1）check lead

voltage is not 5v, see（3）; if voltage is 5v, check
the potential of socket pin 2 of the P273. The
potential is low when sway to reaction cup,
otherwise the potential is high. If the potential is
109
normal, indicator remains, check the adapter
（3）check voltage between P285 plug pin 4，

6, if the voltage is not 5v, check adapter board;
if voltage is 5v, check conductivity from adapter
connected well.
Malfunction 3：Stirring mechanism can reach the

zero position.

Solution:

Rinsing mechanical repair is required if resistance is big.
mechanism of
Rinsing 2, check whether both ends of connector of the
reaction cup fails
3-2 mechanis motor are connected well.
to leave the top
m of when 3, check whether the conductivity of motor lead wire
reaction descending. is good.
cup
abnormal 4, check the motor drive module of circuit board is
working normally
Malfunction 2：Rinsing mechanism of reaction cup
fails to leave the top
solution：the same to that of malfunction 2 in 3-1
110
9.4 Reaction disk

Solution
Code n description
Malfunction：The quantity counted by reaction cup

light sensor does not conform to real quantity of cup
rotated.
solution：Check whether the signal status of counting

Reaction disk light sensor is normal.
Reaction
fails to rotate to
4-1 disk
the designated （1）check whether the conductivity from counting
abnormal
position. light sensor 1 to lead wire is good.
（2）check whether the counting light sensor 1 is ok.
（3）Check input part circuit of reaction disk circuit

Reaction disk Malfunction：Reaction disk fails to stop at the

Reaction
fails to stop at position matching with light sensor.
disk
the designated
abnormal
position solution：the same as 4－1
Malfunction：Reaction disk fails to stop at the zero

position after resetting or the light sensor of zero
position did not check resetting point.
solution：Check whether the signal status of counting

Reaction disk light sensor is normal.
Reaction
fails to stop at
4-3 disk （1）check whether the conductivity from zero light
the zero position
abnormal sensor to lead wire is good and the both ends of
when resetting.
（2）check whether the zero light sensor 1 is ok
（3）Check input part circuit of reaction disk circuit

Liquid level of
cleaning
alkaline
4－6 liquid level Add cleaning liquid into cleaning liquid kit.
cleaning liquid
low
kit low.
111
9.5 Sample probe
Alarm Detailed
Code description

Observe the running status of probe arm.
Malfunction 1：probe arm stops
Solution：
1, move probe mechanism, and mechanical repair is

required if resistance is big.


is good.
4, check the circuit board of sample is working

Sample normally
probe fails to
reach the top
Sample probe
5-1 (except
abnormal. Malfunction 2：Probe arm can rise to zero position.
reaction cup
side) after it
Solution：Manually make sample probe mechanism
rose.
repeatedly rise to peak, and observe ud_s adapter
indicator status.
1、sparkling of ud_s indicator means the wiring of

light sensor and adaptor are normal.
（1）check lead

sample circuit board lead P282-P037 is good and
(2) check sample circuit board
2、no sparkling of ud_s indicator, check ud_s
（1）check lead

circuit board lead （P281-P411） is good and both
112
check voltage between P281plug pin 4，6, if the

high when rose to peak, otherwise the potential is

Malfunction 3：probe arm mechanism can not rise

to the zero position.

Solution：
1, manually move probe mechanism, and mechanical

repair is required if resistance is big.
Sample 2, check whether the flexible cable of liquid level
probe fails to detecting board is connected well.
Sample probe leave the
5-5
abnormal. top(sample 3, check whether both ends of connector of the motor
side) when it are connected well.
descending.
is good.
4,check sample circuit board

5 check whether liquid level detector has
malfunction
Malfunction 2 probe arm leaves zero position

repeatedly rise to peak, and observe ud_s adapter
indicator status.
113
1、sparkling of ud_s indicator means the wiring of
light sensor , lead and adaptor is normal.
（1）check lead

2. if no sparkling of ud_s indicator, check ud_s
（1）check lead


high when light sensor is sheltered, otherwise the
potential is low. If the potential is normal, check the
adapter board; not normal, check the light sensor

6, if the voltage is not 5v, check adapter board;
if voltage is 5v, check conductivity from adapter
connected well.
Sample
probe fails to
Sample probe leave the top
5-6 The solution is as same as 5-5
abnormal. (reaction cup
side) when it
descending.
Malfunction：Sample probe stays in a valid status of

Sample touching or liquid level detecting.
probe stays
in a valid Solution：Sample probe stops .Observe the indicator
Sample probe status when raising probe(Analog touch) manually
5-7 status of
abnormal. and repeatedly and touch probe point (Analog liquid
touching or
liquid level level detection)repeatedly.
detecting.
Initial status：surface_s、touch_s : the touch when it
is bright; touch_s : put out；liquid level detection：
114
surface_s put out.
1、Indicator sparkles normally after repetition of its

movement.
（1）check lead

2、Indicator not sparkle
（1）check whether the flexible cable of liquid

level detecting board is connected well.
（2）check whether the signal lead of liquid level

detection is connected well and its
conductivity is good.
（3）check whether the connector of liquid level

detection is connected well
（4）test the potential of P281 pin 8,9.
P281-8surface is high level, and on-board

indicator goes out when detecting liquid level.
P281-8surface is low level, and on-board

indicator goes out when not detecting liquid
level.
P281-9touch is high level, and on-board

indicator goes out and light sensor is sheltered if
there is any touch.
P281-9touch is low level, and on-board indicator

goes out and light sensor is not sheltered if
there is no touch.
Check adapter if the pin potential of P281 的 8

（surface），9（touch）is normal.
（5）check foot 8（surface），9（touch）of P281

are short circuit with food 7（+5v）, which
makes potential high.
（6）check whether liquid detection board works

well, if not, and please continue to check wire
line board.
Unplug the flexible cables of J6, J7 on liquid

level detection board, measure the feet J6,
115
J7-level status from right to left (J7: 1 empty,
2touch, 3surface) (J6: 1 earth, 2 empty, 3 +5 v,
4 blank)
When detecting the liquid level, J7-3surface is

high level, and on-board indicator is bight;
When not detecting the liquid level,

J7-3surface is low level, and on-board indicator
goes out;
When there is touch, the light sensor is

sheltered, and J7-2touch is high level;
When there is no touch, the light sensor is not

sheltered, and J7-2touch is low level.

Solution：



is good.
Sample 4, check the circuit board of sample is working
probe fails to normally
Sample probe reaction cup
5-8
abnormal. when it 5、check the zero light sensor of up-down mechanism
rotates to is working normally
reaction cup.
Malfunction 2：Rotating arm stops at reaction cup
position.

repeatedly rise to peak, and observe swing_s
adapter indicator status.
1、sparkling of swing_s indicator means the wiring of

（1）check lead

116
2、if no sparkling of swing_s indicator, check

swing_s
（1）check lead

light sensor lead （P281-P411）1、2、3 is good and

high when it stops to the top of reaction cup,
otherwise the potential is low. If the potential is
normal, check the adapter board; not normal, check
the light sensor

6, if the voltage is 5v, check adapter board; if
voltage is not 5v, check conductivity from
adapter board J282 to sample circuit board lead
（P282-P037）is good and both ends of
connector are connected well. If all are right,
check sample circuit board.
Malfunction 3：sample probe can not reach reaction

cup

Observe the running status of sample probe
arm.
Sample Malfunction 1：rotating arm stops
probe fails to
leave solution：
reaction cup
Sample probe
5-9 when it as 5-8
abnormal.
moves from
reaction cup Malfunction 2：rotating arm leaves reaction cup
to other
positions. Malfunction：
as 5-8
117
Sample
probe
Sample probe ¾ Sample probe fails to reach the top and the
5-12 deviates from
abnormal. solution is as 5-1
top when it
rotates.
Malfunction：Probe arm fails to descend to sample

liquid level.
solution：

Sample fails 2, check whether the flexible cable of liquid level

Sample probe to reach detecting board is connected well.
5-14
abnormal. sample
liquid level.
4 check whether the conductivity of motor lead wire

is good.
5, check liquid level detection
6, check the circuit board of sample is working

normally
9.6 Sample disk
Alarm Detailed
Code description
Malfunction 1：sample disk stops
solution：
1, rotate reagent disk, and mechanical repair is required

if resistance is big.
Sample disk
fails to stop 2, check whether both ends of connector of the motor
Sample disk at the are connected well.
6-2
abnormal designated
position of 3, check whether the conductivity of motor lead wire is
outer track. good.
4, check the motor driving unit of sample disk circuit

board is working normally
Malfunction 2：
When sample disk rotates to the designated position,
118
sample disk fails to stop at the designated position or
the light sensor detects it after it reaches the designated
position.
solution：
Manually rotate reagent disk and observe adapter cnt_s

indicator status.
（1）sparkling of cnt_s indicator means the light sensor,

lead and adaptor are normal.
1）check lead

reaction disk circuit board lead is good and both ends
of connector are good.
2）Check input part circuit of reagent disk circuit board

2、if no sparkling of cnt_s, check cnt_s
1）check lead

light sensor lead （P277-P408）is good and both ends
check voltage between P278 plug pin 7，9, if the voltage

is not 5v, see（3）; if voltage is 5v, check the potential of
socket pin 8 of the P278. The potential is high when
rotating to the zero position, otherwise the potential is
low. If the potential is normal, check the adapter board;
not normal, check the light sensor

check conductivity from adapter board J282 to reaction
disk lead（P282-P048）is good and both ends of
connector are connected well. If all are right, check
sample disk circuit board.
Malfunction 1：sample disk stops

Sample disk
solution：
Sample disk fails to find
6-7
abnormal the zero 1, rotate reagent disk, and mechanical repair is required
position. if resistance is big.
119
are connected well.
3, check whether the conductivity of motor lead wire is

good.
4, check the motor driving unit of sample disk circuit

board is working normally
Malfunction 2：
When sample disk rotates to the designated position,

sample disk fails to stop at the designated position or
the light sensor detects it after it reaches the designated
position.
solution：
Manually rotate reagent disk and observe adapter

zero_sdisk indicator status.
（1）sparkling of zero_sdisk indicator means the light

1）check lead

reaction disk circuit board lead is good and both ends of
connector are good.
2）Check input part circuit of reagent disk circuit board

2、if no sparkling of zero_sdisk, check zero_sdisk
1）check lead

light sensor lead （P277-P408）is good and both ends
check voltage between P278 plug pin 1，3, if the voltage

is not 5v, see（3）; if voltage is 5v, check the potential of
socket pin 2 of the P278. The potential is high when
rotating to the zero position, otherwise the potential is
low. If the potential is normal, check the adapter board;
not normal, check the light sensor

reaction disk lead（P282-P048）is good and both
ends of connector are connected well. If all are
120
right, check sample disk circuit board
Malfunction：sample fails to find sample barcode

reader.
Solution:
1, make sure that the barcode device is connected.
2 Switch R1 at the line board side and sample serial

Sample It fails to find data cable, if the bar code reader can not be found,
barcode sample please check the sample disk serial circuit; if it can be
6-10
device barcode found, which means that there is no problem with the
abnormal reader. sample disk and data cable and barcode reader may
exist problem.
3, check the bar code data cable, if the bar code

reader can not be found , which means data cable is
damaged; If can be found , which means barcode
reader is damaged and check the barcode reader.
121
9.7 Sample injection pump
Alarm Detailed
Code description
Malfunction 1：the pump stops or can not reach

the top
solution：
Observe the sample pump running status:
1, When working, the sample pump is not running:
(1) Check whether both ends of connector of the

(2) Check whether the conductivity of motor lead

wire is good.
(3) check the motor drive circuit of circuit board
2、When working, the sample pump is running:

Sample
Sample
injection
7-1 injection pump
pump fails to
abnormal
reach the top (1) check whether light sensor is good
(2) Check whether the conductivity of light sensor

from adapter, reaction disk circuit board lead is
(3) check light sensor signal

voltage is not 5v, see（3）; If voltage is 5v, check the
high when the pump reaches the zero position
(discharging liquid), otherwise the potential is low. If
the potential is normal, check the adapter board; not
(4）check voltage between P282 plug pin 4，6, if

connected well. If all are right, check input part
122
circuit of sample disk circuit board
Malfunction 1：the pump stops or fails to leave the

top
Observe the sample pump running status:
1, When working, the motor is not running:


wire is good.
(3) check the motor drive circuit of circuit board
2、When working, the motor is running:
(1) check whether light sensor is good

Sample
Sample from adapter, reaction disk circuit board lead is
injection
7-2 injection pump good and both ends of connector are good.
pump fails to
abnormal
leave the top (3) check light sensor signal
3. check light sensor signal

voltage is not 5v, see（3）; If voltage is 5v, check the
high when the pump reaches the zero position
4. check voltage between P282 plug pin 4，6, if

connected well. If all are right, check input part
circuit of sample disk circuit board
123
9.8 R1 reagent probe
Alarm Detailed
Code description

check", and observe the reagent probe running
status
solution：
1, pull probe mechanism by hand, mechanical repair

is required if resistance is big.


is good.
4, check the motor drive module of R1 circuit board

is working normally
R1 reagent
probe fails Malfunction 2：probe arm can reach the light sensor,
R1 reagent but light sensor can not detect the signal
to reach
8-1 probe
the top
abnormal solution：Manually make R1 probe mechanism
when it
rises repeatedly rise to top, and observe ud_r1 adapter
indicator status.
1、sparkling of ud_r1 indicator means the wiring of

light sensor, lead and adaptor is normal.
(1) check lead

reagent 1 circuit board is good and both ends of
(2) Check light sensor input circuit of R1 circuit board
2、if no sparkling of ud_r1,check ud_r1
1）check lead

to light sensor lead （P282-P048）is good and both
124

potential of socket pin 5 of the P280. The potential
is high when light sensor is sheltered, otherwise the

reagent 1 circuit board lead（P283-P047）is good and
both ends of connector are connected well. If all are
right, check reagent 1 light sensor position of circuit
board.
Malfunction：Reagent probe collides with the wall or

bottom of battle when it running.
solution：
Observe the reagent probe running status:
Reagent probe collides with the wall or bottom of

battle when it running
R1 reagent Probe （1）the position of reagent probe and bottle need

8－2 probe detects level to be adjusted if the probe collides with bottle wall
abnormal collision when running.
（2）If reagent probe plunges into the bottom of

battle when it is running, check whether there is
reagent in the bottle. If there is no reagent, adding
reagent to test is needed. It there is reagent,
check the circuit board of liquid level detection
and test whether the sensitivity of reagent probe is
normal.

on-line, implement "Resetting", and observe the
R1 reagent reagent probe running status
R1 reagent probe fails to Malfunction 1：probe arm stops
8-3 probe leave the top
abnormal when it solution：
descends
125

is good.

is working normally
Malfunction 2：probe arm can reach the light sensor,

but light sensor can not detect the signal
solution：Manually make R1 probe mechanism

repeatedly rise to top, and observe ud_r1 adapter
indicator status.
1、sparkling of ud_r1 indicator means the light

(1) check lead

reagent 1circuit board （P283-P047）is good and
both ends of connector are connected well.
2、if no sparkling of ud_r1,check ud_r1
1）check lead

to light sensor lead （P282-P048）is good and both


board J283 to reagent 1 circuit board lead
check reagent 1 light sensor position of circuit
board
126
Malfunction： stays the valid status of collision and
liquid level detection
solution：reagent probe arm stops, and pull probe by

hand repeatedly（Analog touch），and repeatedly
touch probe top（Analog liquid level detection），and
observe adapter indicator status.
Initial status：surface_r1、touch_r1: the touch when

it is bright; touch_r1 : put out；liquid level detection：
surface_r1: put out.

movement.
（1）check lead

(2) check sample circuit board or reagent 1

R1 reagent 2、Indicator not sparkle
probe stays
R1 reagent the valid （1）check whether the flexible cable of liquid
8-4 probe status of level detecting board is connected well.
abnormal collision and
liquid level （2）check whether the signal lead（P280-P410）
detection of liquid level detection is connected well and
its conductivity is good.

（4）test the potential of P280 的 8（surface），

9（touch）


level.

there is any touch.

there is no touch.
127


line board.

4 blank)

high level, and on-board indicator is bight;

goes out;



status
R1 reagent Malfunction 1：probe arm stops
probe can
not find the solution：
reaction cup
R1 reagent when it 1, move rotating arm mechanism by hand,
probe rotates to mechanical repair is required if resistance is big.
8-5
abnormal reaction cup. 2, check whether both ends of connector of the
( reagent motor are connected well.
probe can
not reach 3, check whether the conductivity of motor lead wire
zero position) is good.
4, check the motor drive circuit of reagent disk

circuit board is working normally
5, check whether up-down zero light sensor is
working normally.
128

repeatedly rise to the top of reaction cup, and
observe ud_r1 adapter indicator status.
1、sparkling of ud_r1 indicator means the light

(1) check lead

reagent 1 circuit board （P283-P047）is good and
(2) Check R1 circuit board
2、if no sparkling of swing_r1 indicator, check

swing_r1
（1）check lead

light sensor lead （P280-P410）1、2、3 is good and

the light sensor

voltage is 5v, check adapter board; if voltage is not
reagent 1 circuit board lead（P283-P047）is good
and both ends of connector are connected well. If all
are right, check sample circuit board
Malfunction 3：R1 reagent probe fails to leave

reaction cup
solution：mechanical repair
R1 reagent ¾ Enter into the "system maintenance" interface

R1 reagent probe fails to after on-line, implement "mechanical movement
8-6 probe leave check", and observe the R1 running status
abnormal reaction cup Malfunction 1：reagent probe can not sway
when it
129
rotates from solution：As 8-5
reaction cup
Malfunction 2：rotating arm can leave reaction
cup, but light sensor can not detect the signal
solution：As 8-5
R1 reagent
R1 reagent probe
8-7 probe deflects ¾ Probe fails to reach the top and solution is the
abnormal when same as 8-1
rotating
Malfunction：Reagent probe does not detect when

reagent bottle has liquid.
solution：
Liquid level is
R1 reagent not 1、check whether the wiring conductivity of liquid level
8－8 probe detected at detection board and reagent circuit board is good,
abnormal R1 reagent and both ends of connector is connected well.
position
2、check the circuit board of liquid level detection and
test whether the sensitivity of reagent probe is
normal
3、check input circuit of reagent circuit board signal
Malfunction：R1 reagent probe fails to reach liquid

level when it descends
solution
1, move probe mechanism by hand, mechanical

R1 reagent
R1 reagent probe fails to 2, check whether the flexible cable of liquid level
8-9 probe reach liquid detecting board is connected well.
abnormal level when it
descends 3. check whether both ends of connector of the
4. check whether the conductivity of motor lead

wire is good.
5, check the liquid level detection

6. check reagent 1 circuit board
130
9.9 R2 reagent probe
Alarm Detailed
Code description

status
solution：



is good.

is working normally
R1 reagent Malfunction 2：probe arm can reach the light sensor,
R2 reagent but light sensor can not detect the signal
probe fails to
9-1 probe
reach the top
abnormal solution：Manually make R2 probe mechanism
when it rises
repeatedly rise to top, and observe ud_r2 adapter
indicator status.
1、sparkling of ud_r2 indicator means the wiring of

light sensor, lead and adaptor is normal.
(1) check lead

2、if no sparkling of ud_ r2,check ud_ r2
1）check lead

to light sensor lead is good and both ends of
131


board J284 to reagent 2 circuit board lead
check reagent 2 light sensor position of circuit
board.
Malfunction：Reagent probe collides with the wall or

bottom of battle when it running.
solution：
Observe the reagent probe running status:
Reagent probe collides with the wall or bottom of

battle when it running
R2 reagent Probe （1）the position of reagent probe and bottle need

9－2 probe detects level to be adjusted if the probe collides with bottle wall
abnormal collision when running.
（2）If reagent probe plunges into the bottom of

battle when it is running, check whether there is
reagent in the bottle. If there is no reagent, adding
reagent to test is needed. It there is reagent,
check the circuit board of liquid level detection
and test whether the sensitivity of reagent probe is
normal.

on-line, implement "Resetting", and observe the
R2 fails to reagent probe running status
R2 reagent leave the Malfunction 1：probe arm stops
9-3 probe top when
abnormal descendin solution：
g
132

is good.

is working normally

repeatedly rise to top, and observe ud_ r2 adapter
indicator status.
1、sparkling of ud_ r2 indicator means the light

(1) check lead

(2) Check light sensor input circuit of R1 circuit

board
2、if no sparkling of ud_ r2,check ud_ r2
1）check lead

to light sensor lead （P280-P410） is good and


board J284 to reagent 2 circuit board lead is
good and both ends of connector are connected
well. If all are right, check reagent 2 light sensor
position of circuit board
133
Malfunction： stays the valid status of collision and
liquid level detection
solution：reagent probe arm stops, and pull probe by

hand repeatedly（Analog touch），and repeatedly
touch probe top（Analog liquid level detection），and
observe adapter indicator status.
Initial status：surface_r2、touch_r2: the touch when

it is bright; touch_ r2: put out；liquid level detection：
surface_ r2: put out.

movement.
（1）check lead

reagent 2 circuit board lead is good and both ends of
connector are good.
(2) check sample circuit board or reagent 2

R2 reagent 2、Indicator not sparkles
probe stays
R2 reagent the valid （1）check whether the flexible cable of liquid
9-4 probe status of level detecting board is connected well.
abnormal collision and
liquid level （2）check whether the signal lead of liquid level
detection detection is connected well and its
conductivity is good.

（4）test the potential of P279 8（surface），9

（touch）


level.

there is any touch.

there is no touch.
134


line board.

4 blank)

high level, and on-board indicator is bright;

goes out;



status
R2 reagent Malfunction 1：probe arm stops
probe can
not find the solution：
reaction cup
R2 reagent when it 1, move rotating arm mechanism by hand,
probe rotates to mechanical repair is required if resistance is big.
9-5
abnormal reaction cup. 2, check whether both ends of connector of the
( reagent motor are connected well.
probe can
not reach 3, check whether the conductivity of motor lead wire
zero position) is good.
4, check the motor drive circuit of reagent disk

circuit board is working normally
5, check whether up-down zero light sensor is
working normally.
135

repeatedly rise to the top of reaction cup, and
observe ud_ r2 adapter indicator status.
1、sparkling of ud_ r2 indicator means the light

(1) check lead

(2) Check R1 circuit board
2、if no sparkling of swing_ r2 indicator, check

swing_ r2
（1）check lead

light sensor leads 1、2、3 is good and both ends of
connector are good.

the light sensor

voltage is 5v, check adapter board; if voltage is not
5v, check conductivity from adapter board P284 to
sample circuit board
Malfunction 3：R2 reagent probe fails to leave

reaction cup
solution：mechanical repair
R2 reagent
R21 reagent ¾ Enter into the "system maintenance" interface
probe fails to
9-6 probe after on-line, implement "mechanical movement
leave
abnormal check", and observe the reagent probe running
reaction cup
status
when it
136
rotates from Malfunction 1：reagent probe can not sway
reaction cup
solution：As 8-5
Malfunction 2：rotating arm can leave reaction

cup, but light sensor can not detect the signal
solution：As 8-5
R2 reagent
R2 reagent
probe ¾ Probe fails to reach the top and solution is the
9-7 probe
deflects same as 8-1
abnormal
when rotating
Malfunction：Reagent probe does not detect when

reagent bottle has liquid.
solution：
Liquid level is
R2 reagent not detected 1、check whether the wiring conductivity of liquid level
9－8 probe at R2 detection board and reagent circuit board is good,
abnormal reagent and both ends of connector is connected well.
position
2、check the circuit board of liquid level detection and
test whether the sensitivity of reagent probe is
normal
3、check input circuit of reagent circuit board signal
Malfunction：R2 reagent probe fails to reach liquid

level when it descends
solution
1, move probe mechanism by hand, mechanical

R2 reagent
R1 reagent probe fails to 2, check whether the flexible cable of liquid level
9-9 probe reach liquid detecting board is connected well.
abnormal level when it
descends 3. check whether both ends of connector of the
4. check whether the conductivity of motor lead

wire is good.
5, check the liquid level detection

6. check reagent 2 circuit board
137
9.10 R1 reagent disk
Alarm Detailed
Code description
10-1 No No No
Malfunction 1：reagent disk stops
solution：
1, rotate probe mechanism by hand, mechanical



is good.

is working normally
Malfunction 2：
When R1 reagent disk rotates to the designated

position, R1 reagent disk fails to stop at the
R1 reagent
designated position or the light sensor detects it after
disk fails to
R1 reagent it reaches the designated position.
10-2 stop the
disk abnormal
designated solution：
position
cnt_r1 indicator status.
（1）sparkling of cnt_r1 indicator means the light

1）check lead

connector are good.
2）Check input part circuit of reagent disk circuit

2、if no sparkling of cnt_r1,check cnt_r1
1）check lead
138
to light sensor lead is good and both ends of

is high when rotating to the zero position, otherwise
the potential is low. If the potential is normal, check
the adapter board; not normal, check the light sensor

reagent 1 lead（P283-P047）is good and both ends of
reagent 1 circuit board.
¾ Enter into maintenance window and execute

“Mechanical movement check”, and observe its
running status.
solution：
1, rotate reagent disk by hand, and mechanical repair



R1 reagent is good.
R1 reagent disk fails to
10-3
disk abnormal find the zero 4, check the circuit board of reagent 1
position.。
Malfunction 2：
When reagent disk rotates but can not reaches the

designated position.
solution：

zero_r1_disk indicator status.
（1）sparkling zero_r1_disk indicator means the light

139
1）check lead

connector are good.
2）Check it circuit board of reagent 1
2、if no sparkling of zero_r1_disk, check zero_r1_disk
1）check lead



board P283 to reagent 1 lead（P282-P048）is
well. If all are right, check reagent 1 circuit
board
Malfunction：It fails to find R1 reagent disk barcode

reader.
Solution:
2、Check whether the barcode reader is damaged

R1 reagent It fails to find
disk barcode R1 reagent Plug R2 data cable in R1 barcode reader and
10-4 don’t touch circuit board side. Please reset
device disk barcode
abnormal reader. barcode if R1 doesn’t work. If it still doesn’t
work, check barcode reader. Barcode reader
has no problem if R1 works normally, and
problem may occurs on data cable or circuit
board.
3、Check data cable：
Plug R2 serial cable into R1 board. Normal

working of barcode means R1 cable is
damaged and check data cable; check reagent
1 circuit board if barcode can not working
normally.
140
9.11 R2 reagent disk
Alarm Detailed
Code description
solution：
1, rotate probe mechanism by hand, mechanical



is good.

is working normally
Malfunction 2：
When R2 reagent disk rotates to the designated

R2 reagent
position, R2 reagent disk fails to stop at the
disk fails to
R2 reagent designated position or the light sensor detects it after
11-2 stop the
disk abnormal it reaches the designated position.
designated
position solution：

cnt_R2 indicator status.
（1）sparkling of cnt_R2 indicator means the light

sensor, lead and adapter are normal.
1）check lead

connector are good.
2）Check input part circuit of reagent disk circuit

2、if no sparkling of cnt_R2,check cnt_R2
141
1）check lead



reagent 2 lead （P283-P047）is good and both ends
of connector are connected well. If all are right, check
reagent 2 circuit board.
¾ Enter into maintenance window and execute

“Mechanical movement check”, and observe its
running status.
solution：
1, rotate reagent disk by hand, and mechanical repair


R2 reagent
R2 reagent disk fails to 3, check whether the conductivity of motor lead wire
11-3 is good.
disk abnormal find the zero
position.
4, check the circuit board of reagent 2
Malfunction 2：
When reagent disk rotates but can not reach the

designated position.
solution：
142
zero_R2_disk indicator status.
（1）sparkling zero_R2_disk indicator means the light

1）check lead

connector are good.
2）Check it circuit board of reagent 2
2、if no sparkling of zero_R2_disk, check

zero_R2_disk
1）check lead

light sensor lead is good and both ends of connector
are connected well.


reagent 2 lead is good and both ends of connector
are connected well. If all are right, check reagent 2
circuit board
Malfunction：It fails to find R2 reagent disk barcode

reader.
Solution:

It fails to find
R2 reagent R2 reagent 2、Check whether the barcode reader is damaged
11-4
disk abnormal disk barcode
reader. Plug R2 data cable in R1 barcode reader and
don’t touch circuit board side. Please reset
barcode if R2 doesn’t work. If it still doesn’t
work, check barcode reader. Barcode reader
has no problem if R2 works normally, and
problem may occurs on data cable or circuit
board.
3、Check data cable：
Plug R1 serial cable into R2 board. Normal
working of barcode means R2 cable is
damaged and check data cable; check reagent
2 circuit board if barcode can not working
normally.
107
143
9.12 R1 injection pump
Alarm Detailed
Code description
Malfunction：R1injection pump stops or fails to reach

the top
solution：
observe R1 pump running status
1、when working, R1 pump is not running.
(1) check whether both ends of connector of the

(2) check whether the conductivity of motor lead

wire is good.
R1injection R1injection (3)check motor drive circuit of circuit board
14-1 pump pump fails to
abnormal reach the top 2、when working, R1 pump is running：
(1) check light sensor

to adapter and R1 reagent disk circuit board is
well.
（3) check light sensor signal

is high when reaching to the zero
position(discharging liquid), otherwise the potential is
144
（4）check voltage between P283 plug pin 4，6, if

board P283 to R1 reagent circuit board lead is
well. If all are right, check the reagent input
circuit of R1 reagent circuit board.
Malfunction：R1injection pump stops or fails to leave

the top
solution：


wire is good.
(3)check motor drive circuit of circuit board
2、when working, motor works normally and pump

can leave light sensor：
R1injection R1injection (1) check light sensor
14-2 pump pump fails to
abnormal leave the top (2) Check whether the conductivity of light sensor
to adapter and R1 reagent disk circuit board is
well.

is high when reaching to the zero
position(discharging liquid), otherwise the potential is

5v, check conductivity from adapter board P282 to R1
reagent circuit board lead is good and both ends of
the reagent input circuit of R1 reagent circuit board.
145
9.13 R2 injection pump
Alarm Detailed
Code description
Malfunction：R2 injection pump stops or fails to

leave the top
solution：


wire is good.

R2 injection R2 injection (1) check light sensor

15-1 pump pump fails to (2) Check whether the conductivity of light sensor
abnormal reach the top to adapter and R2 reagent disk circuit board is
well.

is high when rotating to the zero position(discharging
liquid), otherwise the potential is low. If the potential
is normal, check the adapter board; not normal,
check the light sensor

5v, check conductivity from adapter board P282 to R1
reagent circuit board lead （P282-P037） is good and
both ends of connector are connected well. If all are
right, check the reagent input circuit of R2 reagent
circuit board.
146
Malfunction：R2 injection pump stops or fails to
leave the top
solution：


wire is good.


R2 injection R2 injection
15-2 pump pump fails to (2) Check whether the conductivity of light sensor
abnormal leave the top to adapter and R2 reagent disk circuit board is
well.

is high when reaching to the zero position
（4）check voltage between P282 plug pin 4，6, if

board P282 to R1 reagent circuit board lead
（P282-P037） is good and both ends of
check the reagent input circuit of R2 reagent
circuit board.
147
9.14 Reaction bath
Alarm Detailed
Code description
(1)Make sure the cooling fan is working normally
(2) check whether temperature sensor is working

Water normally
Water
temperature
temperature
20-1 of reaction (3) check whether the interface of temperature
of reaction
bath is above sensor on main board
bath abnormal
45 degrees
(4) replace control board or temperature sensor
Water (1) Make sure whether the room temperature is within

temperature 15-32 degrees.
of reaction
bath is over (1)Make sure the cooling fan is working normally
Water the rang
temperature （37±0.5
20-2 degrees）
of reaction (3) Make sure whether the water is circulated in
bath abnormal reaction bath
Note: check
only when
(4) replace control board or temperature sensor
operating
148
9.15 ISE
ISE is ISE is over

over measuring
measurin lower limit, and （1）Please check whether the sample
0-31 Note g lower volume, reagent volume is adequate,
limit there is no and whether the position is correct
result of
current QC
ISE is over
ISE is measuring
over lower limit, and （1）Please check whether the sample
0-32 Note measurin volume, reagent volume is adequate,
g lower there is no and whether the position is correct
limit result of
current QC
ISE calibration
ISE is is over
over measuring （1）Please check whether the sample
0-33 Note measurin lower limit, and volume, reagent volume is adequate,
g lower and whether the position is correct
limit there is no
result of
current QC
ISE calibration
ISE is is over
limit there is no
result of
current QC
ISE sample
ISE is is over
limit。 there is no
result of
current QC
149
Electrolyte
system stops
working due to
Electrolyt the issued （1）Enter into system maintenance,
e alarm. Note:
and execute "Resetting”, and then
19-1 Note the status of
function execute “Mechanical movement
stops adding sample
check”
means to
restart.
remaining
volume of
（1）add ISE reference liquid
ISE reference
referenc liquid is （2）Enter into system maintenance,
37-1 Note e liquid is inadequate
and execute "ISE reference liquid
inadequ (less then the
reagent pipeline rinsing”
ate volume user
designed) （3）Execute ISE calibration
remaining
volume of
ISE （1）add ISE internal standard liquid
internal
internal standard liquid （2）Enter into system maintenance,
standard is inadequate
38-1 Note and execute "ISE internal standard
liquid is (less then the
liquid reagent pipeline rinsing”
inadequ volume user
ate designed) （3）Execute ISE calibration
remaining
volume of
diluent （1）add ISE diluent
reference
ISE （2）Enter into system maintenance,
liquid is
39-1 Note diluent is and execute "ISE diluent reagent
inadequate
diluent pipeline rinsing”
(less then the
volume user （3）Execute ISE calibration
designed)
In the (1) Enter into system maintenance,

5-measuring and execute "ISE check."
ISE point
60-1 Note LEVEL
error potential of (2) please refer to " electrolyte device
internal maintenance " in the "user manual" for
standard the detailed
solution, the
150
average value
(EAV)of three
potentials is
over the
following
range
（internal
standard
solution）Na：
-90.0mv≤EAV
≤-10mv
In the
5-measuring
point
potential of
internal
standard
solution, the (1) Enter into system maintenance,
average value and execute "ISE check."
ISE
60-2 Note LEVEL (EAV)of three
error potentials is (2) please refer to " electrolyte device
over the maintenance " in the "user manual" for
following the detailed
range
（internal
standard
solution）K：
-90.0mv≤EAV
≤-10mv
In the
5-measuring
point
potential of
internal (1) Enter into system maintenance,
standard and execute "ISE check."
ISE solution, the
60-3 Note LEVEL average value
error (2) please refer to " electrolyte device
(EAV)of three maintenance " in the "user manual" for
potentials is the detailed
over the
following
range
（internal
standard
151
solution）Cl：
80.0mv≤EAV≤
160mv
In the
5-measuring
point
potential of
internal
standard (1) Enter into system maintenance,
solution, the and execute "ISE check."
ISE difference（FI）
61-1 Note Noise of the
error maximum and (2) please refer to " electrolyte device
minimum is maintenance " in the "user manual" for
within the detailed
following
range
Na：
0.7mv≤|FIV（2）
-FIV（4）|
In the
5-measuring
point
potential of
internal
standard
solution, the (1) Enter into system maintenance,
difference and execute "ISE check."
ISE （FIV） of the
61-2 Note Noise maximum and
error minimum is (2) please refer to " electrolyte device
within maintenance " in the "user manual" for
following the detailed
range (internal
standard,
sample)
K：
1.0mv≤|FIV（2）
-FIV（4）|
In the
(1) Enter into system maintenance,
5-measuring
ISE and execute "ISE check."
point
61-3 Note Noise
error potential of
(2) please refer to " electrolyte device
internal
maintenance " in the "user manual" for
standard
152
solution, the the detailed
difference
（FIV） of the
maximum and
minimum is
within
following
range (internal
standard,
sample)
Cl：0.8mv≤|FIV
（2）-FIV（4）
The slope
value of
calibration
result is within
following
range or the
impact of
ISE electrode is
and execute "ISE check."
Preparati low
62-1 Note on (cross-contami
abnorma nation rate (A) (2) please refer to " electrolyte device
l is as following maintenance " in the "user manual" for
the detailed
Na：（1）
32.0mv≤SLOP
E≤37mv or
68.1mv≤SLOP
E
The slope
value of
calibration
result is within (1) Enter into system maintenance,
ISE following and execute "ISE check."
Preparati range or the
62-2 Note on impact of
abnorma electrode is (2) please refer to " electrolyte device
l low maintenance " in the "user manual" for
(cross-contami the detailed
nation rate (A)
is as following
K：（1）
32.0mv≤SLOP
E≤37mv
or68.1mv≤SL
OPE
153
The slope
value of
calibration
result is within
following (1) Enter into system maintenance,
range or the and execute "ISE check."
impact of
ISE
electrode is
Preparati
low (2) please refer to " electrolyte device
62-3 Note on
(cross-contami maintenance " in the "user manual" for
abnorma
nation rate (A) the detailed
l
is as following
Cl：（1）
-30mv≤SLOPE
≤-25mv
or-68.1mv≥SL
OPE
（1）The slope
value of
calibration
result is within
following
range or the (1) Enter into system maintenance,
impact of and execute "ISE check."
electrode is
low
ISE (cross-contami (2) please refer to " electrolyte device
SLOPE nation rate (A) maintenance " in the "user manual" for
63-1 Note
abnorma is as following: the detailed
l
Na：
（1）SLOPE
＜32.0mv (3) Please re-execute ISE calibration.
（2）current IS
calibration
result is not
updated
（1）The slope (1) Enter into system maintenance,

ISE
value of and execute "ISE check."
SLOPE
63-2 Note calibration
abnorma
result is within
l (2) please refer to " electrolyte device
following
range or the maintenance " in the "user manual" for
154
impact of the detailed
electrode is
low
(cross-contami (3) Please re-execute ISE calibration.
nation rate (A)
is as
following:K：
（1）SLOPE＜
32mv
（2）current IS
calibration
result is not
updated
（1）The slope
value of
calibration
result is within
following (1) Enter into system maintenance,
range or the and execute "ISE check."
impact of
electrode is
ISE low (2) please refer to " electrolyte device
SLOPE (cross-contami maintenance " in the "user manual" for
63-3 Note
abnorma nation rate (A) the detailed
l is as
following:Cl：
（1）SLOPE＞ (3) Please re-execute ISE calibration.
-25.0mv
（2）current IS
calibration
result is not
updated
（1）the
concentration
of internal (1) Enter into system maintenance,
liquid（C（IS）） and execute "ISE check."
ISE the is within
concentr following
ation of (2) please refer to " electrolyte device
range :Na：C maintenance " in the "user manual" for
64-1 Note internal
（IS）＜ the detailed
liquid
abnorma 120.0mmol/l or
l 190.0mmol/＜
C（IS）。 (3) Please re-execute ISE calibration.
（2）current IS
calibration
result is not
155
updated
（1）the
concentration
of internal
liquid（C（IS）） (1) Enter into system maintenance,
is within and execute "ISE check."
following
ISE the
concentr range .K：C
ation of （IS）＜ (2) please refer to " electrolyte device
64-2 Note internal 3.0mmol/l or maintenance " in the "user manual" for
liquid 8.0mmol/＜C the detailed
abnorma （IS）。
l (3) Please re-execute ISE calibration.
（2）current IS
calibration
result is not
updated
（1）the
concentration
of internal
liquid（C（IS））
and execute "ISE check."
is within
ISE the following
concentr range Cl：C
ation of (2) please refer to " electrolyte device
（IS）＜ maintenance " in the "user manual" for
64-3 Note internal
liquid 80.0mmol/l 或 the detailed
abnorma 140.0mmol/＜
l C（IS）。
(3) Please re-execute ISE calibration.
（2）current IS
calibration
result is not
updated
Because of the
implementatio
n of the ISE
maintenance
(ISE cleaning,
complete Executive ISE calibration
cleaning), it is
necessary to
implement ISE
calibration
156
Alarm Detailed
Code description
Malfunction 1： pump stops or fails to reach the top
solution：
observe pump running status
1、when working, injection pump is not running.


wire is good.

18-1
2、when working, injection pump is running：
SIP injection SIP injection (1) check light sensor

pump pump fails to (2) Check whether the conductivity of light sensor
abnormal reach the top to adapter and ISE circuit board is good and both
check voltage between P901A plug pin 1，3

(measured when plug it into plug seat), if the voltage
is 5v, check potential (measured when plug it into
plug seat)of P901A plug pin 2，3; The potential is high
when reaching to the zero position(discharging liquid),
normal, Check whether the conductivity of light
sensor to adapter and ISE circuit board is good; if not
normal, replace the light sensor
If the voltage is not 5v, check voltage between

P190 plug pin 1，3; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.
157
Alarm Detailed
Code description
Malfunction 1： injection pump stops or fails to

leave the top
solution：


wire is good.

18-2 SIP injection SIP injection
pump pump fails to (2) Check whether the conductivity of light sensor
abnormal leave the top to adapter and ISE circuit board is good and

when reaching to the zero position(discharging
is normal, Check whether the conductivity of light

P190 plug pin 1，3; if voltage is 5v, replace light
18-3 DIL injection DIL injection

pump pump fails to
abnormal reach the top reach the top
158
Alarm Detailed
Code description
solution：


wire is good.

to adapter and ISE circuit board is good and both

plug seat)of P903Aplug pin 2，3; The potential is high
when reaching to the zero position(discharging liquid),

P190 plug pin 7,9; if voltage is 5v, replace light

18-4 DIL injection DIL injection leave the top
pump pump fails to
solution：
abnormal leave the top
159


wire is good.

to adapter and ISE circuit board is good and

when reaching to the zero position (discharging

160
Alarm Detailed
Code description

reach the top
IS injection IS injection solution：

pump pump fails to
abnormal reach the top


wire is good.

to adapter and ISE circuit board is good and both

liquid), otherwise the potential is low. If the potential is

161
Alarm
Code Detailed
description

leave the top
solution：
18-6 IS injection IS injection observe pump running status

pump pump fails to


wire is good.

to adapter and ISE circuit board is good and


162
Alarm
Code Detailed
description
Malfunction 1： Electromagnet stops or fails to

reach the bottom
16-1 solution：
observe Electromagnet running status

The nozzle of
SIP nozzle
electrolyte
fails to reach 1、when working, Electromagnet is not running.
SIP
the bottom
abnormal
Electromagnet are connected well.
(2) Check whether the conductivity of

Electromagnet lead wire is good.
(3)check Electromagnet drive circuit of circuit board
2、when working, Electromagnet is running：
（1）check whether the block separates the light

sensor when the electromagnet has descended.
If not separated, adjust block till separate the
light sensor.

ISE circuit board is good and both ends of
connector are connected well. 。
check voltage (measured when plug it into plug seat)

between P904 plug pin 4，6, if the voltage is 5v, check
voltage (measured when plug it into plug seat)of P904
plug pin 5，6; The potential is high when solenoid
valve is located low, otherwise the potential is low. If
the potential is normal, Check whether the
conductivity of light sensor ISE circuit board is good; if
not normal, replace the light sensor

163
Alarm Detailed
Code description

leave the top
solution：

pump pump fails to 1、when working, Electromagnet is not running.

（1）check whether the block left the light sensor

when the electromagnet has risen. If not left, adjust
block till left the light sensor.

check light sensor signal

between P904 plug pin 4，6,
if the voltage is 5v, check voltage (measured when

plug it into plug seat)of P904 plug pin 5，6; The
potential is high when solenoid valve is located low,
normal, Check whether the conductivity of light sensor
ISE circuit board is good; if good, check light sensor
input circuit of ISE circuit board;. If the potential is not
If the voltage is not 5v, Check whether the

conductivity of light sensor ISE circuit board is
good; if good, check light sensor input circuit of
ISE circuit board
164
Alarm
Code Detailed
description

17-1 reach the bottom
solution：
The nozzle of SIP nozzle observe Electromagnet running status

electrolyte SIP fails to reach
abnormal the bottom
1、when working, Electromagnet is not running.


（1）check whether the block separates the light

sensor when the electromagnet has descended.
If not separated, adjust block till separate the
light sensor.

connector are connected well. 。

between P904 plug pin 1，3, if the voltage is 5v, check
voltage (measured when plug it into plug seat)of P904
plug pin 2，3; The potential is high when solenoid
valve is located low, otherwise the potential is low. If
the potential is normal, Check whether the
conductivity of light sensor ISE circuit board is good; if
not normal, replace the light sensor

165
leave the top
solution：

pump pump fails to 1、when working, Electromagnet is not running.

（1）check whether the block left the light sensor

when the electromagnet has risen. If not left, adjust
block till left the light sensor.

check light sensor signal

between P904 plug pin1，3,
if the voltage is 5v, check voltage (measured when

plug it into plug seat)of P904 plug pin2，3; The
potential is high when solenoid valve is located low,
normal, Check whether the conductivity of light sensor
ISE circuit board is good; if good, check light sensor
input circuit of ISE circuit board;. If the potential is not

166
9.16 Resetting and others
Alarm
Code Detailed
description
Sending time
Time
synchronizati Check the communications of control board and
143-1 synchronizat
on order sub-boards respectively.
ion failure
failure
Water tank
Water Check whether the water supply machine, solenoid
liquid
adding valve, pipeline and filter are working normally, and
143-2 system
overtime check whether the water supply pipe has air, and
malfunction,
error check whether the floater is normal, and check
water
whether relevant electrical units of floater and control
overtime
board are normal.
error
AD board
detects
Use control panel monitoring software to monitor the
malfunction
AD board fault information to see if there is AD communication
143-3 when
reset failure error. Repair AD board if there is AD communication
resetting, AD
error to debug.
board reset
failure。
Reaction
disk detects
malfunction
Reaction when Lower machine monitoring software monitors error
143-4 disk reset resetting, information, and analyze the reaction disk
failure board-related failure to eliminate the error
Reaction
disk reset
failure。
Sample disk
detects
Sample disk malfunction Use lower machine monitoring software to monitor
143-5 reset when error information, and refer to the related information
failure resetting, of sample disk board to debug.
sample disk
fails to reset。
167
R1 disk
detects
malfunction Use lower machine monitoring software to monitor
R1 disk
143-6 when error information, and refer to the related error
reset failure
resetting, R1 information of R1 board to debug.
disk fails to
reset
R2 disk
detects
malfunction Use lower machine monitoring software to monitor
R2 disk
143-7 when error information, and refer to the related error
reset failure
resetting, R2 information of R2 board to debug.
disk fails to
reset
Check whether the indication of reaction bath liquid

Water Water
level detector is right; check whether there is
dischargin dischargin
remaining water in reaction bath, and check reaction
143-8 g failure of g failure of
bath water outlet pipeline if there is remaining water
reaction reaction
in it; check whether relevant electrical units of liquid
bath bath
level detection and control board are normal.
R1 and R2
Connect the main control board debugging program
reagent
to electrify the machine; observe machine electrifying
probes fails
Adding flow; observe whether the 6 times the normal
to add
detergent cleansing movement of detergents reagent 1 reagent
143-9 detergent
overtime 2 is finished in the phase of adding detergents.
completely
error Corresponding control board of reagent probe should
in the
be repaired if the reagent probe did not achieve
specified
normal movement.
time
R1 fails to
R1liquid
detect liquid
level
143-10 level when Refer to liquid detection error analysis to debug
detection
adding
failure
detergent
R2 fails to
R2 liquid
detect liquid
level
143-11 level when Refer to liquid detection error analysis to debug
detection
adding
failure
detergent
168
Liquid level Check the liquid level of reaction bath, and check
Liquid level
detection whether the liquid level detector of reaction bath
detection
143-12 failure of works normally; check whether relevant electrical
failure of
reaction units of liquid level detection and control board are
reaction bath
bath normal.
Check whether the liquid level detector of reaction

bath is clean, whether there is water in reaction bath,
Reaction Reaction
whether water level reaches the position where the
bath liquid bath liquid
143-13 detector can detect, and check liquid level detector in
level level
reaction bath is working normally; check whether
malfunction malfunction
relevant electrical units of liquid level detection and
control board are normal.
Bar code Bar code

Use lower machine monitoring software to monitor
scanning scanning
143-14 error information, and refer to the related error
overtime overtime
information of reagent and sample disks to debug.
error error
Execute the main board debugging program to

monitor, and re-execute pipeline air exhausting and
Pipeline Pipeline observe whether air exhausting is carried out by
exhaust exhaust reagent and sample injection pumps and the
143-15
overtime overtime implementation is complete. Use lower machine
error error monitoring software to monitor error information, and
refer to the related error information of reagent and
sample disks to debug.
Reaction Reaction Use lower machine monitoring software to monitor

143-16 disk start disk start error information, and refer to the related error
failure failure information of reaction disk to debug.
Reaction Reaction Use lower machine monitoring software to monitor

143-17 disk stop disk stop error information, and refer to the related error
Maintenance operation of the sample probe. Check

Sample Sample whether the pressure sensor and solenoid valve of
143-18 probe probe sample probe pipeline are normal; check whether
blocked blocked relevant electrical units of pressure detection and
Previous Previous
sample sample
adding adding
information of sample disk to debug.
failure failure
Previous Previous
reagent 1 reagent 1
adding adding
information of reagent 1 to debug.
failure failure
169
Previous Previous
reagent 2 reagent 2
adding adding
failure failure
Previous Previous
reagent 3 reagent 3
adding adding
failure failure
Previous Previous Use lower machine monitoring software to monitor

143-23 reagent 4 reagent 4 error information, and refer to the related error
adding adding information of reagent 1 to debug.
failure failure
143-24 stirring 1 stirring 1 error information, and refer to the related error



waste liquid bottle and control board are normal.
Check whether waste liquid bottle is full; check
143-28 Waste liquid Waste liquid
whether the sensor in waste liquid bottle is normal;
bottle full bottle full
check whether relevant electrical units of the floater in
Switch
malfunction, Check whether the floaters of high and low liquid level
high-level and the signal cable connecting floater to main
143-29 Floater floater control board are normal; check whether relevant
switch error detects the electrical units of floater detection and control board
signal, but are normal.
low-level
floater fails.
Reagent Reagent
level level Use lower machine monitoring software to monitor
143-30 scanning scanning error information, and refer to the related error
overtime overtime information of reagent board to debug.
error error
170
Check whether the vacuum pump and vacuum pump
Vacuum
Vacuum switch are normal; check whether relevant electrical
Pump
143-31 Pump units of vacuum pump switch and control board are
negative
Failure normal.
pressure low
Sample Sample
barcode barcode Use lower machine monitoring software to monitor
143-32 scanning scanning error information, and refer to the related error
overtime in overtime in information of sample board to debug.
testing testing
ISE detects
Observe whether there is ISE alarm information;
malfunction
ISE reset Use lower machine monitoring software to monitor
143-33 in resetting;
failure error information, and refer to the related error
resetting
information of ISE board to debug.
failed.
ISE check
ISE check Observe whether there is ISE alarm information; Use
maintenanc
maintenance lower machine monitoring software to monitor error
143-34 e
movement information, and refer to the related error information
movement
overtime of ISE board to debug.
overtime
ISE pipeline
ISE pipeline rinsing is Observe whether there is ISE alarm information; Use
143-35 rinsing over lower machine monitoring software to monitor error
overtime specified information, and refer to the related error information
time of ISE board to debug.
ISE
ISE malfunction Observe whether there is ISE alarm information; Use
143-36 malfunction when testing, lower machine monitoring software to monitor error
in testing follow-up ISE information, and refer to the related error information
stops of ISE board to debug.
Check gear pump and pressure sensor of sample

pipeline are normal. Check whether the read value of
pressure sensor which is running is more than 2500
Gear pump Gear pump
143-37 by using main control board debugging software;
failure pressure low
check whether relevant electrical units of pressure
sensor and control board are normal.
Sending
reagent
Sending
mapping
reagent
information
143-42 mapping Refer to instrument module error analysis
failure,
information
adding
failure
reagent may
fail.
171
When the
water tank
temperature
is over 36.5
degrees, add
cold water
into the tank
observe whether the display of temperature is normal
into to cool
cooling and room temperature is high; check the water supply
down.
143-43 water pipeline of water tank is normal; check whether
Temperature
overtime relevant electrical units of temperature sensor and
could not
drop to 35.5
degrees in
one minute,
which means
cold water
temperature
is too high.

Analyzer Malfunction monitor whether the communications of main board
143-44 module among and sub-boards are normal; check whether relevant
malfunction modules electrical units of sub-boards communications and
Check whether light source, water quality of

Continuous
Continuous incubation bath, reaction cup and counting light
emergence
143-45 emergence sensor of reaction disk are normal; test data
of 5 dirty
of dirty cups collecting board lines by using the testing program of
cups
data collecting board.
AD data AD data Execute the main board debugging program to

malfunction mixed monitor whether the communications of main control
143-46
board and AD board is normal, and analyze error;
check whether counting light sensor is normal.
IES ISE Use lower machine monitoring software to monitor

malfunction measuring error information, and refer to the related error
143-47 in testing internal information of ISE board to debug.
standard
liquid failure

Version Version
monitor whether the communications is normal, and
number number
143-48 analyze error; check whether relevant electrical units
reading reading
of sub-boards communications and control board are
overtime overtime
normal.
172
9.17 Cooling system
Alarm
Code Detailed
description
(1)check whether reagent disk cover is covered,

whether the ambient temperature is in line with
environmental requirements of instrument.
Cooling system Cooling time (2) observe the temperature displayed on digital
144-1
abnormal abnormal pipe of cooling system and Peltier current value are
normal.
If abnormal: Please check Peltier and cooling

circuit board
（1）observe whether current value displayed on

Cooling digital pipe of cooling system is normal.
Cooling system
144-2 current
abnormal （2）Make sure which current is abnormal, and cope
abnormal
with it after check the corresponding abnormal Peltier
and circuit board.
（1）observe which chip is abnormal

Cooling system Cooling chip
144-3
abnormal abnormal
（2）cope with the corresponding abnormal chip
（1）check the communications wiring of cooling

board and main board
Cooling Cooling
144-5 communication status （2）check the communications interface circuit of
abnormal abnormal
cooling board
（3）check the communications interface circuit of

main board
The 1st line The 1st line Check the 1st line cooling Peltier and cooling chip
145-1 cooling chip cooling
malfunction current <5A
The 2nd line The 2nd Check the 2nd line cooling Peltier and cooling chip
145-2 cooling chip line cooling
The 3rd line The 3rd line Check the 3rd line cooling Peltier and cooling chip
The 4th line The 4th line Check the 4th line cooling Peltier and cooling chip
173
9.18 AD collector
Alarm Detailed
Code description
The 1st line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-1 1st line AD
collector is
over normal
range
The 2nd line The Check the AD collection board and preamp board
146-2 2nd line AD
collector is
over normal
range
The 3rd line The Check the AD collection board and preamp board
146-3 3rd line AD
collector is
over normal
range
The 4th line The Check the AD collection board and preamp board
146-4 4th line AD
collector is
over normal
range
The fifth line The Check the AD collection board and preamp board
146-5 fifth line AD
collector is
over normal
range
The sixth line The Check the AD collection board and preamp board
146-6 sixth line AD
collector is
over normal
range
174
The seventh The Check the AD collection board and preamp board
line AD measuring
collector value of the
146-7 malfunction seventh line
AD collector
is over
normal range
The eighth The Check the AD collection board and preamp board
line AD measuring
collector value of the
146-8 malfunction eighth line
AD collector
is over
normal range
The ninth line The Check the AD collection board and preamp board
146-9 ninth line AD
collector is
over normal
range
The tenth The Check the AD collection board and preamp board
line AD measuring
146-10 collector value of the
malfunction tenth line
AD collector
is over
normal range
146-11 11th line AD
collector is
over normal
range
146-12 12th line AD
collector is
over normal
range
175

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Specification Model: CS-400

① Machine model logo

③Left front door

④ Right front door

1-1-3 Front Open Picture of Analyzer

① SIP (reference), injection pump

② DIL (Diluted Release) injection pump

③ IS (internal standard), injection pump

④ CS-alkaline cleaning liquid box

③ left back cover board

⑤ light liquid outlet

⑥ right back cover board

⑦ purified water entrance

⑧ concentrated liquid waste outlet

1-1-5 The Top of Analyzer

② reaction cup cleaning mechanism

③ reaction disk mechanism

④ reaction bath liquid detection

⑥ R1 reagent adding mechanism

⑦ probe cleaning reagent groove

⑨ sample disk rotating indicator

⑩ sample disk inner refrigerated cover

⑿ R2 reagent adding mechanism ⑨ ⑩ ⑾ ⑿⒀ ⒁

① Analytical unit switches

(not including refrigeration power supply)

② refrigeration power supply indicator（green）

③ main power supply（breaker）

1-2-1 The Structure of Analyzer

Sample disk Barcode window

115 sample positions，including：

Routine sample position： 50

STAT sample position ： 20

Standard solution position: 34

Cleaning liquid position： 3

R1disk：only for R1and R4

R2disk：only for R2 and R3

Position 45 of two reagent disk:

Only for phosphor-free cleaning liquid

Reagent bottle volume：70mland 20ml

1-2-4 Reaction Unit

Reaction cup：120，optical path: 6mm

20×6 sets hard optical plastic

Digital liquid sensor

7-stop 11-step rinsing of colorimetric cup

1sample probe, 2 reagent probes

High-precision digital liquid detector

High-speed hollow cup motor

Surface high-intensity Teflon coating

1-2-6 Precise Distributing Pump （Injection Pump）

② Colorimetric unit：3 （sample，reagent R2，reagent R1）

③ Colorimetric cup original rinsing liquid box

1-2-7 Control unit 1

① circuit panel boxes: 6 panels

Order: from left to right

Reaction disk control panel

Sample disk control panel

Reagent R1 control panel

Reagent R2 control panel