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    Chromatography Lab

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    ashleygaboy86
    This document provides the procedure and background for a paper chromatography lab to separate and identify plant pigments like chlorophyll, carotene, and anthocyanin found in leaves. Students will extract and chromatograph pigments from mayana and dahon kamote leaves using acetone as the solvent to separate the mixtures based on differences in solubility and mobility. The results will be analyzed to determine the makeup of pigments in each plant sample.

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    Chromatography Lab

    Uploaded by

    ashleygaboy86
    50 views3 pages
    This document provides the procedure and background for a paper chromatography lab to separate and identify plant pigments like chlorophyll, carotene, and anthocyanin found in leaves. Students will extract and chromatograph pigments from mayana and dahon kamote leaves using acetone as the solvent to separate the mixtures based on differences in solubility and mobility. The results will be analyzed to determine the makeup of pigments in each plant sample.

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    Chromatography-Lab

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    This document provides the procedure and background for a paper chromatography lab to separate and identify plant pigments like chlorophyll, carotene, and anthocyanin found in leaves. Students will extract and chromatograph pigments from mayana and dahon kamote leaves using acetone as the solvent to separate the mixtures based on differences in solubility and mobility. The results will be analyzed to determine the makeup of pigments in each plant sample.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    50 views3 pages

    Chromatography Lab

    Uploaded by

    ashleygaboy86
    This document provides the procedure and background for a paper chromatography lab to separate and identify plant pigments like chlorophyll, carotene, and anthocyanin found in leaves. Students will extract and chromatograph pigments from mayana and dahon kamote leaves using acetone as the solvent to separate the mixtures based on differences in solubility and mobility. The results will be analyzed to determine the makeup of pigments in each plant sample.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    COLEGIO DE SAN JOSE, JARO, ILOILO CITY, INC.

    E. Lopez St., Brgy. Our Lady of Fatima, Jaro, Iloilo City


    Contact Numbers: (033) 329-1595 or 329-4151  Fax Number: (033) 329-6629
    Member: Daughters of Charity-St. Louise de Marillac Educational System

    INTEGRATED BASIC EDUCATION DEPARTMENT

    FIRST QUARTER GENERAL CHEMISTRY 1


    LABORATORY WORKHEET 2
    School Year 2023-2024

    Name: ________________________________________Grade 12 STEM-Sec.: ___________


    Teacher: ______________________________________ Date: _______________________

    I. Title: Plant Pigment Chromatography


    II. Objectives:
    At the end of the activity, you will be to:
    1. Follow directions in order to properly complete a paper chromatography.
    2. Analyze the results of a chromatography experiment in order to determine the makeup of plant
    pigment
    3. Appreciate the importance of paper chromatography

    III. Background Information:


    Paper chromatography is a technique used to separate a mixture into its component molecules. The
    molecules migrate, or move up the paper, at different rates because of differences in solubility, mass,
    and bonding.

    The following pigments can be found in a spinach leaf (Malabar spinach):


    Chlorophyll a  bright green Carotene  orange Anthocyanin- red, purple
    Chlorophyll b dull (khaki) green Xanthophyll  yellow

    IV. Materials:
    1. Laboratory Gown & gloves 7. Laboratory Journal
    2. Mayana Leaves & Dahon Kamote (plenty) 8. Scissor
    3. 250 mL beaker 9. Dropper (suspension medicine)
    4. Acetone (nail-polish remover) 10. Broomstick / big paper clip(1pc)
    5. Pencil (sharpened) 11. Adhesive tape (masking tape)
    6. Ruler 12. Mortar and pestle
    13. Rugs (cleaning materials)
    14. Hand towel

    V. Procedure:
    1. Use mortar and pestle to grind up roughly equal samples of each of the different plant leaves.
    Label beaker for each type of plant.
    2. Extract leaves purely without putting any liquids in ut.
    3. Cut each chromatography paper in half (length-wise) to make approximately 2 centimeters (cm)
    wide by 7.5 cm long strips. You will need 2 chromatography strips.
    4. Use a pencil to lightly label which leaf extract will be spotted on each paper strip.
    Tip: do not use a pen for writing on the strips: the ink will run when the solvent passes through the
    strips.
    5. Draw a pencil line 1 cm from the edge of each strip of paper to be the origin of the baseline
    6. Take a dropper and fill it with one of the plant leaf extracts.
    7. Spot the sample in the middle of the origin line. Let the sample air dry. Then apply another layer of
    the sample on the same spot. Ideally, you want to repeat this step 5–10 times to increase the
    concentration of your sample on the paper strip.
    8. Repeat step 7 with the other plant leaf extracts you want to test. Use a new paper strip for each
    one.
    9. Fill the test tube to a depth of 1 cm with the acetone (nail-polish remover).
    10. Clip two of the prepared chromatography strips to a broomstick. Make sure the two strips do not
    touch each other or the test tube and that their bottoms are aligned. Rest the broomstick on top of
    the beaker so that the strips hang straight into the test tube.
    11. If necessary, add more of the acetone. The goal is to have the end of the chromatography strip
    just touching the surface of the solvent.
    (Write in the Laboratory Journal)

    Group # _____________ Date: ___________

    Paper Chromatography

    I. Result (Illustration with label)


    II. Discussion of the Result
    Guide Questions:
    1. Why are two solvents used in the process?
    2. Justify the separation of the bands
    3. Which pigment is the MOST soluble in the solvent? Explain your answer.
    4. Which pigment is LEAST soluble in the solvent? Explain your answer.
    5. Which pigment band is the widest?
    6. Which pigment band is the most intense (darkest)?

    III. Conclusions
    IV. Recommendations

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