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    Lab Report

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    NUR AISHAH MOHAMAD
    MS. NOR AZLIZA BINTI ISMAIL DATE OF SUBMISSION: 23 MAY 2022

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    Lab Report

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    NUR AISHAH MOHAMAD
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    MS. NOR AZLIZA BINTI ISMAIL DATE OF SUBMISSION: 23 MAY 2022

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    LAB REPORT

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    MS. NOR AZLIZA BINTI ISMAIL DATE OF SUBMISSION: 23 MAY 2022

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    35 views6 pages

    Lab Report

    Uploaded by

    NUR AISHAH MOHAMAD
    MS. NOR AZLIZA BINTI ISMAIL DATE OF SUBMISSION: 23 MAY 2022

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 6

    FAKULTI SAINS GUNAAN

    UNIVERISITI TEKNOLOGI MARA


    CAWANGAN PERAK KAMPUS TAPAH

    BIO150 – METABOLISM & CELL DIVISION

    SCIENTIFIC LAB REPORT

    NAME: NUR AISHAH BINTI MOHAMAD

    STUDENT ID: 2021608348

    GROUP: AS1202C

    EXPERIMENT TITLE: ENZYME 1: THE FASTEST ENZYME-CATALASE

    LECTURER: MS. NOR AZLIZA BINTI ISMAIL

    DATE OF SUBMISSION:

    ---------------------------------------------------------------------------------------------------------------------------
    Declaration of Academic Honesty

    Academic honesty or academic integrity is a very important virtue that all students should
    uphold at all times.

    I declare that the lab report submitted is not plagiarised and is entirely my own work and that
    no part of it has been copied from any work produced by another person (s)/ source(s) or
    provided by any other student(s).

    I understand that issuing a false declaration can result in severe penalties and I am willing to
    be penalized if any form of copying is found valid.
    Introduction

    Instead of good chemicals, human body cells also produce poisonous chemicals, but
    they won’t damage nor kill the cells. It’s because the cells use enzymes to break down these
    poisonous chemicals into harmless substances. Enzymes are proteins that speed up the
    rate of reaction that would otherwise happen more slowly. The enzyme will not change by
    the reaction. They are big protein molecules that have specialized processes in the cell. In
    the presence of light, hydrogen peroxide decomposes slowly to create oxygen and water.
    This process can be sped up/catalyzed by the enzyme catalase.

    Catalase enzyme breaks down hydrogen peroxide (H2O2) into water and oxygen.

    2H2O2 catalase 2 HO2 + O2

    Hydrogen peroxide is a highly active chemical that is produced continuously in living


    cells as a by-product of chemical processes. It's toxic, and if the cells don't break it down
    right away, it'll destroy them. As a result, the enzyme's relevance is well understood.

    Objective

    1. Identify the factors that influence the reactions of the catalase enzyme.
    2. Analyse the reaction of catalase on different samples.

    Apparatus

    1. 7 test tubes
    2. Test tubes holder
    3. Rubber stopper
    4. Toothpick
    5. Pastel and mortar
    6. Beaker 250 cm3
    7. Thermometer
    8. Bunsen burner

    Material

    1. Hydrogen peroxide
    2. Fresh sample liver
    3. Fresh sample potato
    Procedure

    1. 2cm3 of hydrogen peroxide solutions was poured into a clean test tube. 1 cm cube
    of the liver was cut and dropped into the test tube of hydrogen peroxide. The
    reaction was observed carefully and recorded. The presence of oxygen was
    tested with a splint.
    2. A piece of liver of the same size (1cm cube) was placed in a mortar. The liver
    was ground, and the ground-up liver was transferred into a test tube containing
    fresh hydrogen peroxide. The activity of the ground liver was noted and the
    activity that has been observed for the whole cube of liver prepared earlier was
    compared.
    3. Another piece of liver was put in a beaker of boiling water for three minutes. The
    piece of liver was dropped into fresh hydrogen peroxide. Observation of whether
    the enzyme was still capable of breaking down the hydrogen peroxide was
    recorded.
    4. Another experiment was carried out with another fresh sample of potatoes to find
    out if they contain catalase enzyme.
    5. The result was recorded in Table 1 (APPENDIX 1).
    RESULTS
    APPENDIX I Table 1: Catalase reaction
    Test tube containing Observations Presence of Oxygen

    Cube of liver • More bubbles formation Yes


    • The wooden splinter keeps burning

    Ground liver • Huge amount of bubble formation Yes


    • The wooden splinter keeps burning
    brightly

    Boiled liver • Less to no bubble formation No


    • The wooden splinter didn’t burn

    Cube of potato • More bubbles formation Yes


    • The wooden splinter lights up for a
    few seconds

    Ground potato • Huge bubbles formation Yes


    • The wooden splinter lights up for a
    few seconds
    Boiled potato • Less bubble formation No
    • The wooden splinter didn’t light up

    NAME: NUR AISHAH BINTI MOHAMAD


    STUDENT ID: 2021608348

    VERIFIED BY:
    DISCUSSION

    Based on the data recorded, a larger surface area influences the rate of catalase
    response. The grounded samples are more reactive than the cube-shaped ones. because
    more oxygen is created from the breakdown of hydrogen peroxide, the bigger the surface
    area of the sample, the faster the process. When the samples of potato and liver are cooked
    at high temperatures, there are fewer bubbles formed, and the wooden splinters light up for
    a brief time, indicating that no oxygen is created. This is because enzymes denature at
    extreme temperatures over 37ºC due to the shape of the active site. As the liver works to
    detoxify substances in the body, it possesses more catalase than potatoes. This is shown by
    the time the wooden splinter lights up longer when the sample of the liver was used
    compared to when the sample of potato was used.

    The errors that might have occurred in the experiment are the unnoticeable result
    that had occurred in the experiment. The samples used should be in a bigger amount to get
    more significant results.

    CONCLUSION

    In conclusion, the increase in surface area and the effect of temperature are two
    factors that influence catalase response. The catalase response on the liver sample is more
    reactive than the reaction on the potato sample because the wooden splinter burns longer
    when the liver sample is used versus when the potato sample is used. This also
    demonstrates that the liver contains more catalase than the potato, allowing it to create more
    oxygen. As the result, the catalase reaction on both liver and potato samples is investigated
    and experiment goals have been met.
    REFERENCE

    • Education.com. (2013, November 18). Catalase and hydrogen peroxide experiment:


    Science Project. Science project | Education.com. Retrieved May 23, 2022, from
    https://www.education.com/science-fair/article/activator/
    • MKki2la2, T. A. P. A. H. B. I. O. L. O. G. Y. (2020, October 20). Experiment 1 -
    enzyme I - the fastest enzyme: Catalase. YouTube. Retrieved May 23, 2022, from
    https://www.youtube.com/watch?v=yeYbCMWrEJI
    • Ahmad Mustamin, K. (2017, October 15). Course. Academia.edu. Retrieved May 23,
    2022, from https://www.academia.edu/34867912/Course
    • Buddies, S. (2016, November 10). Exploring enzymes. Scientific American. Retrieved
    June 1, 2022, from https://www.scientificamerican.com/article/exploring-enzymes/

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