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Biotechnology Principles and Processes, Biotechnology and Its Applications-Split

This document contains multiple choice questions about biotechnology principles and processes, including restriction endonucleases. It tests knowledge about the following key points: 1) Restriction endonucleases are molecular scissors that cut DNA at specific sequences. The first one isolated was HindII, which cuts at a 6 base pair sequence. 2) EcoRI is a restriction endonuclease isolated from the bacteria E. coli RY13. It recognizes and cuts a specific palindromic nucleotide sequence. 3) Restriction fragments produced by digestion can be separated by gel electrophoresis, allowing visualization of cut DNA. 4) Stanley Cohen was the first to link antibiotic resistance genes to plasmids, a key step in the

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26 views

Biotechnology Principles and Processes, Biotechnology and Its Applications-Split

This document contains multiple choice questions about biotechnology principles and processes, including restriction endonucleases. It tests knowledge about the following key points: 1) Restriction endonucleases are molecular scissors that cut DNA at specific sequences. The first one isolated was HindII, which cuts at a 6 base pair sequence. 2) EcoRI is a restriction endonuclease isolated from the bacteria E. coli RY13. It recognizes and cuts a specific palindromic nucleotide sequence. 3) Restriction fragments produced by digestion can be separated by gel electrophoresis, allowing visualization of cut DNA. 4) Stanley Cohen was the first to link antibiotic resistance genes to plasmids, a key step in the

Uploaded by

kksah0200
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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Biotechnology Principles and Processes, Biotechnology and its

Applications
PART-1 (SECTION-A) (1) BamH I
1. Which of the following is a biotechnological (2) Sal I
product- (3) Hind II
(1) Vaccine (4) EcoR I
(2) Enzymes 9. Hind II cuts DNA at a specific sequence of
(3) Cheese (1) 5 base pairs
(4) All of these (2) 7 basepairs
2. Making multiple copies of any template DNA in a (3) 6 base pairs
host cell is known as (4) 8 base pairs
(1) Replication 10. In the nomenclature of restriction endonuclease
(2) Transcription enzyme the first letter and the successive two
(3) Reverse transcription letters denote
(4) Cloning (1) Strain, Genus
3. Given below are two statements : one is labelled (2) Genus, strain
as Assertion (A) and the other is labelled as Reason (3) Genus, species
(R) (4) Strain, species
Assertion (A) : EcoRI comes from Escherichia coli 11. EcoR I is isolated from
RY13 (1) E.coli RY11
Reason (R) : In EcoRI the letter 'R' is derived from (2) E.coli RY13
the name of statin (3) E.coli RY10
In the light of the above statements, choose the (4) E.coli RY15
correct answer from the options given below : 12. What is correct about Hind-II
(1) Both (A) and (R) are correct but (R) is not the correct (1) It is the first restriction endonuclease
explanation of (A) (2) Its functioning depends on a specific DNA
(2) (A) is correct but (R) is not correct nucleotide sequence
(3) (A) is not correct but (R) is correct (3) It cuts DNA by identifying a specific sequence of
(4) Both (A) and (R) are correct and (R) is the correct six base pairs
explanation of (A) (4) All of these
4. Stanley Cohen isolated the antibiotic resistance 13. The speci fic sequence recognized by
gene and linked it to a native plasmid of endonuclease is called-
(1) Salmonella typhimurium (1) Parallel sequence
(2) Salmonella pneumoniae (2) Palindromic nucleotide sequence
(3) Saccharomyces cerevisiae (3) Polynucleotide sequence
(4) E. coli (4) None of these
5. Molecular scissors are- 14. The fragments of DNA produced by restriction
(1) Helicases digestion are separated by
(2) Restriction endonucleases (1) Chromatography
(3) Ligases (2) Gel electrophoresis
(4) Topoisomerases (3) Centrifugation
6. Which of the following is a vector (4) None of these
(1) Plasmid 15. Given below are two statements :
(2) Cosmid Statement I : In the year 1963, the three enzymes
(3) Phagemid responsible escherichia coli were isolated.
(4) All of these Statement II : The first restriction endonuclease -
7. The new DNA molecule generated by the linking Hind II Choose the correct answer from the options
of foreign DNA to vector is called given below
(1) Plasmid DNA (1) Both Statement I and Statement II are incorrect
(2) Recombinant DNA (2) Statement I is correct but Statement II is incorrect
(3) Combination DNA (3) Statement I is incorrect but Statement II is correct
(4) Chromosomal DNA (4) Both, Statement I and Statement II are correct
8. The first restriction endonuclease isolated was 16. Under an electric field DNA moves towards :

By – Anmol Sharma Sir 1|P a g e

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