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Narrative Report

The document describes a student's experience in a bacterium culture laboratory session. The activities included culturing bacteria and fungi as well as analyzing bacteria under a microscope. Bacteria were cultured using a streak plate method and grew in distinct colonies on agar plates. Fungi were cultured by placing tissue onto agar and grew as a powdery mat. Gram staining and microscopy were used to view and identify bacteria. Though challenging, the student was able to successfully complete the assigned laboratory activities and learned about culture techniques.

Uploaded by

Albert Bagasala Asido
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© © All Rights Reserved
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Download as DOCX, PDF, TXT or read online on Scribd
237 views

Narrative Report

The document describes a student's experience in a bacterium culture laboratory session. The activities included culturing bacteria and fungi as well as analyzing bacteria under a microscope. Bacteria were cultured using a streak plate method and grew in distinct colonies on agar plates. Fungi were cultured by placing tissue onto agar and grew as a powdery mat. Gram staining and microscopy were used to view and identify bacteria. Though challenging, the student was able to successfully complete the assigned laboratory activities and learned about culture techniques.

Uploaded by

Albert Bagasala Asido
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Republic of the Philippines

PARTIDO STATE UNIVERSITY


Camarines Sur

NARRATIVE REPORT

Name: ASIDO, ALBERT B. Date: February 19, 2024


Course/ Year&Sec: BSSE 3A

"Bacterium Culture Session"

Activities:

● To culture Bacteria
● To culture Fungi
● Bacteria Analysis under a Microscope

I. Introduction

Bacteria are an essential part of the ecosystem. Have an important role in food
production. However, they can also be harmful, causing damage and disease.
Microbiological culture, or microbial culture, is a method of multiplying microbial
organisms by letting them reproduce in predetermined culture medium under controlled
laboratory conditions. Microbial cultures are foundational and basic diagnostic methods
used as research tools in molecular biology.

On that day, February 19 in the present year. We are assigned to conduct a


laboratory activity lead by the laboratory personnel.I was quiet feeling kind of nervous
that day on what activity that we're going to do and I was thinking that I haven't done it
for almost a long period of time, laboraty experiments but for the sake of new learning
and experience, I had the vision to just enjoy what will happen and what will be done
for the rest of the day. As an starting point, laboratory equipments are clearly
introduced, their respective names and how to handle/use it for the laboratory purposes.
I am already familiar with the other laboratory equipments nevertheless, some
equipments introduced are quiet not faniliar for me. Gladly I have given a chance and to
be one of the students that been tought about supplementary equipment .

1
The following activities are brightly been discussed the step by step process by
laboratory personnel assocaitive with care. The following activity is individually
perfomed resulting to different outcome.Here's what I've got.

II. Activities

CULTURING BACTERIA

We used the commonly approach which is the three quadrant streak method, which
consists of the following steps:

1. Inoculate the first quadrant of an agar plate.


2. Streak the bacteria in quadrant two, overlapping quadrant one for two to three
streaks.
3. Streak the bacteria in quadrant three, overlapping quadrant two for two to three
streaks.
4. And lastly, incubate the plate.

Result after a day:

We can see that bacteria grow in colonies—groups of cells—on agar plates. A


single bacterium or a small number of bacteria give rise to each colony. Masses of cells
can be seen, but individual cells are too tiny to be seen. Different forms, edges,
altitudes, and colors are possible for colonies.

2
CULTURING FUNGI

Procedure:
● Plate fungal tissue onto agar.
● Take a fruiting body of P. ostreatus and tear it down the middle.
● Sterilize your forceps with some ethanol and flame over alcohol lamp flame.
● After forceps have cooled, take part of the trama tissue from the fruiting body.
Avoid the surface areas of the fungi, as these are most likely to have bacteria
and other nasties on them that can contaminate your culture.
● Place fruiting body down, and with you free hand slightly (not all the way) remove
the lid of the petri plate, being careful to avoid touching the edges. Be sure to
keep your face distant from the plate as not to breathe any contaminants onto the
agar.
● Gently place the tissue in the middle of the plate. Replace cover.
● We are advised to see the result after 5 days.

All over the agar plate, we can see that fungi us fully develop as a powdery mats

In conclusion,bacteria readily flourish on nutrient-rich culture conditions, in contrast


to fungus. Numerous bacterial and fungal species create colonies with phenotypically
different appearances. The colonies differ in terms of size, form, texture, color, and
margins. To study colony morphology, bacteria and fungi should be grown on agar in
Petri dishes with all the necessary nutrients and conditions.The main difference
between fungal and bacterial colonies is that fungal colonies are visible masses of
fungus that arise from a single spore or mycelial fragment, whereas bacterial colonies
are identifiable masses of bacterial cells developing from single bacterial cells.

3
BACTERIA ANALYSIS

The last activity is the analyzing a bacteria under a microscope. We followed certain
process inorder to get a better output when it comes to determining it under the
microscope. It started by putting a drop of water in a test plate, followed by getting a
small dot of sample agar and mix it into the water until fully dissolve and lastly heat until
dry.Next step is Gram straining process.Staining helps to highlight the bacteria's
characteristics and provides contrast against the background.

Gram staining process includes:

● Applying a primary stain (crystal violet,1minute).


● Adding a mordant (Gram's iodine,1 minute).
● Rapid decolorization with ethanol, acetone or a mixture of both. (10 secs)
● Counterstaining with safranin. (30 secs)

Lastly, analyzing the bacteria using a microscope. Here are the steps.

● Begin by setting up the microscope on a clean, sturdy surface.


● Put the sample test plate
● Mounting the Slide.
● Lower the Objective Lens.
● Focus on the Sample.
● Increase Magnification.
● Observe and Identify

It was a little bit hard to get the final output under the microscope . Maybe I wasn't
not good in using it.Nevertheless, luckily and glady found it at last.

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