This document provides instructions for using a zinc colorimetric test kit to measure zinc levels in samples. It details the test principle, reagents, procedure, standard values, precautions, and more. The test uses a reagent to create a complex with zinc that is measured spectrophotometrically.
This document provides instructions for using a zinc colorimetric test kit to measure zinc levels in samples. It details the test principle, reagents, procedure, standard values, precautions, and more. The test uses a reagent to create a complex with zinc that is measured spectrophotometrically.
This document provides instructions for using a zinc colorimetric test kit to measure zinc levels in samples. It details the test principle, reagents, procedure, standard values, precautions, and more. The test uses a reagent to create a complex with zinc that is measured spectrophotometrically.
This document provides instructions for using a zinc colorimetric test kit to measure zinc levels in samples. It details the test principle, reagents, procedure, standard values, precautions, and more. The test uses a reagent to create a complex with zinc that is measured spectrophotometrically.
For 50 tests R 1 x 50 ml Zinc Colorimetric test, 5-Br-PAPS method
Liquid Reagent Test principle Calculation Zinc reacts with 2-(5-bromo-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino) -phenol (5-Br-PAPS) to form a red chelate complex in pH = 9.8 . The A Sample Conc. Zinc (g/dl) = X Conc. STD.( g/dl) increase of absorbance measure at 560 nm is proportional to the A STD. concentration of total zinc in the sample. Concentrations in the test X 0.153 g/dl mol/L Reagent R X 6.537 Bicarbonate buffer, pH = 9.8 500 mmol/L Linearity 5-Br-PAPS 0.03 mmol/L Up to 400 g/dl (61.2 mol/L). Sodium citrate 68 mmol/L If the result exceeds 400 g/dl, repeat the test using diluted sample Dimethyl glyoxime 4 mmol/L (1+2) with sodium chloride solution (0.9 %) and multiply the result by 3. Detergent Standard : The concentration as indicated on vial. Precautions 1. Use only disposable plastic containers or iron free tubes and cuvettes. Stability of reagent Avoid any contamination by the use of clean laboratory material. Reagent : Liquid, ready for use. 2. Reagent contains sodium azide. Don’t swallow. Avoid any contact with Stability:Up to expiry date given on the label when stored at skin and mucous membranes. Sodium azide may react with lead and +2 +8°C. copper plumbing to form explosive metal azides. Upon disposal, flush Specimen collection and handling with large amounts of water to prevent azide build up. 1. Non-hemolyzed serum and plasma. Reference range 2. Heparinized plasma could only be used, other anticoagulants should not be used. Free of hemolayz. Serum: g/dl mol/l 3. Blood specimen should be collected in trace element blood collection tube. <4 mth. 65 - 137 10 - 21 4. Remove serum from clot as soon as possible. 4 – 12 mth. 65 - 130 10 - 20 5. Stability in serum: 7 days at 2 – 8 °C. 6. 24/ hr. Urine: Collect in clean, plastic urine container with no metal 1 – 5 yr. 65 - 118 10 - 18 cap or glued insert. Refrigerate after completion of collection. 7. Seminal fluid: Centrifuge the sample at 3000 rpm for 10-15 min. 6 – 9 yr. 78 - 105 12 - 16 Stability of the sample 7 days at 2 – 8 °C. Dilute supernatant men 78 - 98 12 - 15 (1+99) with sodium chloride solution (0.9 %) and multiply the result 10 – 13 yr. by 100. women 78 - 118 12 - 18 Standard men 65 - 118 10 - 18 Zinc STD. Cat. No. 16221 14 – 19 yr. Quality control women 59 - 98 9 - 15 Meditrol N Cat. No. 15171 Adults 46 - 150 7 - 23 Meditrol P Cat. No. 15181 Procedure Urine: ( 300 – 800 ) mg/24hr. Wavelength Hg 546 nm Spectrophotometer 560 nm 2 – 10 mg/dL Cuvette 1 cm light path Seminal fluid 2000 – 10000 g /dL Temperature 20 – 25 °C Measurement Against reagent blank Reaction end point Note: Serum zinc levels are generally 5 -15 % higher than plasma levels Assay due to zinc released from platelets and erythrocytes during clotting. Blank Standard Sample Reference Double dist. water 50 l -- -- 1. Johansen and R.Eliasson. Evaluation of a commercially available kit for colorimetric determination of zinc. International Jornal of andrology, 1987, Standard -- 50 l -- April 10 (2) : 435 - 440. Sample -- -- 50 l 2. Young, DS., Effects of Drugs on Clinical Laboratory Tests, fifth editiopn 2000, AACC Press, Washington, D.C. Reagent 1000 l 1000 l 1000 l Mix, incubate for 8 min. at 20 – 25 °C. Read the absorbance (A). The final colour is stable for at least 30 min.
In vitro diagnostics First edition 2010
Medichem Middle East Aleppo, Syria, E-mail: info@medichem-me.com
