Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Scribd Logo
Upload

Welcome to Scribd!

  • 12 views

    Microbiology Practical Ospe

    Uploaded by

    It's AJ
    The document provides information about a microbiology practical exam, including questions on identifying specimens under gram stain, biochemical tests, culture media, and microscopic examination. Sample questions are provided on slide identification, organism identification based on test results, identifying media and how it is prepared and sterilized, identifying and describing specimens, and interpreting results of various microbiology tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Microbiology Practical Ospe

    Uploaded by

    It's AJ
    12 views87 pages
    The document provides information about a microbiology practical exam, including questions on identifying specimens under gram stain, biochemical tests, culture media, and microscopic examination. Sample questions are provided on slide identification, organism identification based on test results, identifying media and how it is prepared and sterilized, identifying and describing specimens, and interpreting results of various microbiology tests.

    Original Description:

    Information regarding microbiology OSPE under SSUHS

    Original Title

    Microbiology practical ospe

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document provides information about a microbiology practical exam, including questions on identifying specimens under gram stain, biochemical tests, culture media, and microscopic examination. Sample questions are provided on slide identification, organism identification based on test results, identifying media and how it is prepared and sterilized, identifying and describing specimens, and interpreting results of various microbiology tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    12 views87 pages

    Microbiology Practical Ospe

    Uploaded by

    It's AJ
    The document provides information about a microbiology practical exam, including questions on identifying specimens under gram stain, biochemical tests, culture media, and microscopic examination. Sample questions are provided on slide identification, organism identification based on test results, identifying media and how it is prepared and sterilized, identifying and describing specimens, and interpreting results of various microbiology tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 87

    Microbiology

    Practcal exam
    Spottg
    Questot 1:
    I. Identfy the slide
    II. Primary stain/counter stain/decolouriser/example of such
    organism

    1. Gram stain:
    • Gram positve cocci/bacilli/bacilli with spores
    • Gram negatve bacilii/coccobacilli/cocci
    • Yeast: Gram positve yeast cells (Gram satain)

    2. Ziehl-Neelsen stain: Acid fast bacilli


    Gram positve cocci arratged it clusters
    Gram positve cocci arratged it pairs atd short chaits
    Gram positve yeast cells seet
    Gram positve spore bearitg bacilli
    Gram positve bacilli
    Gram tegatve bacilli
    Gram tegatve coccobacilli
    Gram tegatve cocci
    Acid fast bacilli seet
    Questot 2
    1. Identfy the biochemical test
    2. One organism giving positve test/indicator used
    Itdole ritg is formed

    Indole ring is formed Positve & negatve


    Urea is hydrolysed
    Citrate is utlised
    Triple sugar irot agar test: H2S productot

    K/A, H2S positve


    Glucose fermettatot test with productot
    of acid otly
    Sugar fermettatot test
    Sugar fermettatot test
    Sugar fermettatot test
    • Identfy the medium: Glucose/Sucrose/maltose/mannitol/lactose
    fermentaton test with producton of acid only/acid and gas

    • Indicator used

    • Percentage of indicator

    • Name of the tube inside

    • One organism giving such results


    Questot 3
    • Idettfy the medium
    • How it is sterilized/use/type of media
    Nutrient agar Blood agar

    MacConkey agar Chocolate agar


    C/A, N/A,M/A, B/A
    Lowetsteit-Jetset medium: how it is
    sterilised, use, type: selectve media
    Robertsot’s cooked meat medium: use,
    compositot
    Questot 4
    • Idettfy the specimet
    • Use/how sterilised etc (accorditg to specimet)
    Idettfy, use, how sterilised
    Idettfy, use, sterilisatot
    Idettfy, use, type of media, sterilisatot, tame of other
    tratsport media
    Idettfy, sterilisatot, use

    Loefers serum slope

    SDA medium
    Test tube, swab stck, CB medium, LSS, SDA medium
    LSS & CB medium
    Swab stck & test tube
    Idettfy
    Idettfy, use
    Identfy, use, sterilisaton
    Questot 5
    • Identfy the specimen
    • Intermediate host/Defnitve host or portal of entry or any queston
    related to disease/life cycle
    A.
    B.
    C.
    D.
    Viva table
    • Biochemicals
    • Culture media
    • Specimens
    • BMW bags
    BMW bags: idettfy, what type of waste disposed,
    type of treatmett to waste disposed
    • Yeast: white to cream coloured smoth surface

    • Moulds:
    – colour of the surface,
    – surface: aerial mycelium/gratular,
    – reverse: colour/aty pigmettatot
    Moulds
    SDA medium
    Moulds
    Yeasts
    Yeasts
    Glucose broth & bile broth: blood culture
    Idettfy, uses, attcoagulatt used, collectot
    of blood specimet
    Vials
    LF/NLF
    LF/NLF
    Alpha & beta hemolysis
    Beta, alpha and no hemolysis
    McIttosh Filde’s jar: use
    Catdle jar: use
    Itoculattg loop, how will you sterilise?
    Practcal questot 1. Gram stait
    • Culture plate
    • Biochemicals
    Set for E. coli
    Set for Klebsiella pteumotiae (ote urease
    extra)
    Set for Bacillus subtlis
    Set for Staphylococcus aureus
    Staphylococcus aureus: B/A (beta
    hemolysis)
    Bacillus subtlis: N/A
    E. Coli: M/A
    K. pteumotiae: M/A
    P. aerugitosa: NA/MA

    Nutrient agar
    P. aerugitosa: NA/MA

    MacConkey agar
    2 No practcal: AFB stait
    • Draw diagram whatever you have seen under microscope:
    • If positve: grading
    • If no AFB seen: negatve
    3 No practcal questot
    • Draw diagram of egg seen under 10X, 40X
    Egg of Trichuris
    Ascaris
    Eggs of Ascaris
    Ascaris: Utfertlised eggs
    Hookworm
    Widal test reck
    • Viva
    Rapid kit test
    • For Malarial parasite
    • RPR
    Thank you

    You might also like