Arpit Done

CERTIFICATE
This is to certify that ARPIT KUMAR GUPTA,

student of class 12th science, has successfully
completed research given below in the project under
the heading “STUDY OF QUANTITY OF CASEIN IN DIFFERENT
SAMPLE OF MILK” during session 2024-2025 under the
guidance of VARSHA TRIPATHI.
---------------------- -----------------------
VARSHA TRIPATHI ARPIT KUMAR GUPTA

ACKNOWLEDGEMENT
I would like to express my immense gratitude to
my chemistry teacher VARSHA TRIPATHI, for
the help and guidance she provided for completing
the investigatory project.
I also thank my parents who gave their ideas and

inputs in making this project. Most of all I thank
our school management, for providing us the
facilities and opportunity to do this project.
Lastly, I would like to thank my schoolmates who

have rendered and done this project along with me.
Their support made this project fruitful.
- ARPIT KUMAR GUPTA

INDEX
 AIM OF EXPERIMENT
 OBJECTIVE
 INTRODUCTION
 APPLICATIONS
 THEORY
 APPARATUS REQUIRED
 CHEMICAL REQUIRED
 PROCEDURE
 OBSERVATION
 RESULT
 PRECAUTION
 BIBLIOGRAPHY
AIM
TO STUDY THE QUANTITY

OF CASEIN IN DIFFERENT
SAMPLE OF MILK.
OBJECTIVE
To study the quantity present in different samples

of milk.
INTRODUCTION
Milk is a complete diet as it contains proteins,

carbohydrates, fats, minerals, vitamins and water.
The average composition of milk from different
source is given below:
SOURCE WATER MINERALS FATS CARBO PROTIENS

OF MILK (%) (%) (%) HYDRATEDS (%)
(%)
COW 87.1 0.7 3.9 4.9 3.4

HUMAN 87.4 0.2 4.0 4.9 1.4
GOAT 87 0.7 4.2 4.8 3.3
SHEEP 82.6 0.9 6.5 4.5 5.5
Casein is the most predominant phosphorote is

found in milk and cheese. When coagulate with
rennet, casein is sometimes called paracasein.
British terminology, on the other hand, uses the
term caseinogens for the uncoagulated protein and
casein for coagulated protein. As it exists in milk, it
is a salt of calcium.
Casein is not coagulated by heat. It is precipitated

by acids and by rennet enzymes, a proteolytic
enzyme typically obtained from the stomachs of
calves. The enzyme trypsin can hydrolyze off a
phosphate containing peptone.
 Casein consists of a fairly high number of praline

peptides, which do not interact. There are also no
disulphide bridges. As a result, it has relatively
little secondary structure or tertiary structure.
Because of this, it cannot denature. It is relatively
hydrophobic, making it poorly soluble in water. It
is found in milk as a suspension of particles called
casein micelles which show some resemblance
with surfactant-type micelle in a sense that the
hydrophilic parts reside at the surface. The caseins
in the micelles are held together by calcium ions
and hydrophobic interactions. These micelles have
negative charge and on adding acid to milk the
negative charges are neutralized.
Ca2+ -
Caesinate + 2CH3COOH(aq) Casein+(CH3COO)2Ca(aq)
 The isoelectric point of casein is 4.7. The

purified protein is water insoluble. While it is also
insoluble in neutral salt solutions, it is readily
dispersible in dilute alkalis and in salt solutions
such as sodium oxalate and sodium acetate.
APPLICATIONS
 In addition to being consumed in milk, casein
in used in the manufacture of adhesives, binders,
protective coatings, plastics (such as for knife
handles and knitting needles), fabrics, food
additives and many other products. It is commonly
used by bodybuilders as a slow-digestive source of
amino acids as opposed to the fast-digesting whey
protein, and also as an extremely high source of
glutamine (post workout). Another reason it is
used in bodybuilding, is because of its anti-
catabolic effect, meaning that casein consumption
inhibits protein breakdown in the body. Casein is
frequently found in otherwise nondairy cheese
substitutes to improve consistency especially when
melted.
THEORY
 Milk contains 3 to 4% casein suspended in
water in the Colloidal form. It is precipitated in a
weakly acidic medium.
APPARATUS-REQUIRED
 Funnel
 Funnel stand
 Glass rod
 Filter paper
 Weight box,
 Test tubes,
 Pestle
 Mortar.
Chemicals-Required
 Different samples of milk.
 Saturated ammonium sulphate solution.
 1 % acetic acid solution.
Procedure
 Wash the beaker (250 ml) with the distilled water
and dry it.
 Take 20 ml of buffalo’s milk in 250 ml beaker and
find its weight.
 Add 20 ml saturated solution of ammonium
sulphate slowly with stirring. Fat and casein will
separate out as precipitate.
 Filter the above solution and transfer the
precipitate in another beaker.
 Treat the above precipitate with 30 ml distilled
water. Casein dissolves forming milky solution
whereas fat remains as such.
 Warm the above contents of the beaker to 40 -
45°C on a low flame. Now, add 1% acetic acid
solution drop wise with stirring when casein gets
precipitated.
 Filter the precipitated casein and wash with
distilled water and dry it.
 Find the weight of dry precipitate.
 Repeat the whole experiment with cow’s milk,
goat’s milk and sheep’s milk.
OBSERVATIONS
 Volume of milk taken in each case = 20 ml
 Weight of milk taken = W₁ g
 Weight of Casein isolated = W₂ g
 Percentage of casein = Weight of Casein x 100
Weight of milk
S. TYPE OF VOLUM WEIGH WEIGH PERCENTAG

NO MILK E OF T OF T OF E OF CASIEN
. MILK MILK CASIEN
TAKEN (W1g) (W2g)
(ml)
1. Buffalo’ 20 ml 23.09 0.632 2.73%
s milk
2. Cow’s 20 ml 35.66 0.55 1.64%
milk
3. Goat’s 20 ml 23.09 0.77 3.67%
milk
Result
 Different Samples of milk contains different
percentage of casein.
 Highest percentage of casein is present in
Goat’s milk.
PRECAUTIONS
 Handle apparatus and chemicals carefully.
 Add ammonium sulphate solution very slowly.
 Stir milk while adding chemicals.
 Do not disturb milk after adding ammonium
sulphate solution and wait some time for fat and
casein to precipitate out.
 Take the amount readings carefully with digital
weighing machine only.
BIBLIOGRAPHY
 Class 12th Chemistry NCERT book.
 Pradeep’s Chemistry class 12th.
 Class 12th chemistry comprehensive practical lab

manual.

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CERTIFICATE

This is to certify that ARPIT KUMAR GUPTA,

VARSHA TRIPATHI ARPIT KUMAR GUPTA

I also thank my parents who gave their ideas and

Lastly, I would like to thank my schoolmates who

- ARPIT KUMAR GUPTA

TO STUDY THE QUANTITY

To study the quantity present in different samples

Milk is a complete diet as it contains proteins,

SOURCE WATER MINERALS FATS CARBO PROTIENS

COW 87.1 0.7 3.9 4.9 3.4

Casein is the most predominant phosphorote is

Casein is not coagulated by heat. It is precipitated

 Casein consists of a fairly high number of praline

 The isoelectric point of casein is 4.7. The

S. TYPE OF VOLUM WEIGH WEIGH PERCENTAG

 Pradeep’s Chemistry class 12th.

 Class 12th chemistry comprehensive practical lab

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