G 3 Amount of Casein in Milk Chemistry Project

2023 - 2024
Chemistry Investigatory Project
NAME:
Class XII
Roll No. -
2014-2015
Objective –
To study the quantity of Casein present

in different samples of milk.
Introduction –
Milk is a complete diet as it contains proteins,
carbohydrates, fats, minerals, vitamins and water. The
average composition of milk from different sources is given
below:
SOURCE OF WATER MINERALS PROTEINS FATS CARBO-HYDRATES

MILK
(%) (%) (%) (%) (%)
Cow 87.1 0.7 3.4 3.9 4.9
Human 87.4 0.2 1.4 4.0 4.9
Goat 87 0.7 3.3 4.2 4.8
Sheep 82.6 0.9 5.5 6.5 4.5
Casein is the most predominant phosphoprote is found

in milk an cheese. When coagulated with rennet, casein is
sometimes called Paracasein. British terminology, on the
other hand, uses the term caseinogen for the uncoagulated
protein and casein for coagulated protein. As it exists in milk,
it is a salt of calcium.
Casein is not coagulated by heat. It is precipitated by
acids and by rennet enzymes, a proteolytic enzyme typically
obtained from the stomachs of calves. The enzyme trypsin
can hydrolyze off a phosphate-containing peptone.
Casein consists of a fairly high number of praline
peptides, which do not interact. There are also no disulphide
bridges. As a result, it has relatively little secondary structure
or tertiary structure. Because of this, it cannot denature. It is
relatively hydrophobic, making it poorly soluble in water. It is
found in milk as a suspension of particles called casein
micelles which show some resemblance with surfactant-type
micellae in a sense that the hydrophilic parts reside at the
surface. The caseins in the micelles are held together by
calcium ions and hydrophobic interactions. These micelles
have negative charge and on adding acid to milk the negative
charges are neutralized.
Ca2+ - Caesinate + 2CH3COOH(aq)  Casein+(CH3COO)2Ca(aq)
The isoelectric point of casein is 4.7. The purified protein

is water insoluble. While it is also insoluble in neutral salt
solutions, it is readily dispersible in dilute alkalis and in salt
solutions such as sodium oxalate and sodium acetate.
Applications:
In addition to being consumed in milk, casein in used in

the manufacture of adhesives, binders, protective coatings,
plastics (such as for knife handles and knitting needles),
fabrics, food additives and many other products. It is
commonly used by bodybuilders as a slow-digestive source of
amino acids as opposed to the fast-digesting whey protein,
and also as an extremely high source of glutamine (post
workout). Another reason it is used in bodybuilding, is
because of its anti-catabolic effect, meaning that casein
consumption inhibits protein breakdown in the body. Casein
is frequently found in otherwise nondairy cheese substitutes
to improve consistency especially when melted.
Aim –
To study quantity of casein in different samples of milk.
Theory –
Milk contains 3 to 4% casein suspended in water in the
colloidal form. It is precipitated in a weakly acidic medium.
Apparatus Required –
Funnel, funnel stand , glass rod , filter paper, weight box ,
test tubes, pestle and mortar.
Chemicals Required –
(i) Different samples of milk.
(ii) Saturated ammonium sulphate solution.
(iii) 1 % acetic acid solution.

Procedure –
1. Wash the beaker (250 ml) with the distilled water and dry it.
2. Take 20 ml of buffalo’s milk in 250 ml beaker and find its weight.
3. Add 20 ml saturated solution of ammonium sulphate slowly with

stirring. Fat and casein will separate out as precipitate.
4. Filter the above solution and transfer the precipitate in another

beaker.
5. Treat the above precipitate with 30 ml distilled water. Casein

dissolves forming milky solution whereas fat remains as such.
6. Warm the above contents of the beaker to 40 - 45°C on a

low flame. Now, add 1% acetic acid solution drop wise with
stirring when casein gets precipitated.
7. Filter the precipitated casein and wash with distilled water

and dry it.
8. Find the weight of dry precipitate.
9. Repeat the whole experiment with cow’s milk, goat’s milk

and sheep’s milk.
Observations –
Volume of milk taken in each case = 20 ml
Weight of milk taken = W₁ g
Weight of Casein isolated = W₂ g
Percentage of casein = Weight of Casein x 100

Weight of milk
S.no. Type of milk Volume of Weight Weight of Percentage of

milk taken of milk Casein casein
(ml) (W₁ g) (W₂ g)
1. Buffalo’s 20 23.09 0.632 2.73%

milk
2. Cow’s milk 20 35.66 0.55 1.64%
3. Goat’s milk 20 23.09 0.77 3.67%

Result –
Different Samples of milk contains different percentage

of casein.
Highest percentage of casein is present in Goat’s milk.
Precautions –
1. Handle apparatus and chemicals carefully.
2. Add ammonium sulphate solution very slowly.
3. Stir milk while adding chemicals.
4. Do not disturb milk after adding ammonium
sulphate solution and wait some time for fat and
casein to precipitate out.
5. Take the amount readings carefully with digital
weighing machine only.
Bibliography –
www.wikipedia.com
www.encyclopedia.com
www.caesine-pro.com
www.sciencejournals.com
www.icar.nic.in
www.zetascience.com

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2023 - 2024

Chemistry Investigatory Project

To study the quantity of Casein present

SOURCE OF WATER MINERALS PROTEINS FATS CARBO-HYDRATES

Cow 87.1 0.7 3.4 3.9 4.9

Human 87.4 0.2 1.4 4.0 4.9

Goat 87 0.7 3.3 4.2 4.8

Sheep 82.6 0.9 5.5 6.5 4.5

Casein is the most predominant phosphoprote is found

Ca2+ - Caesinate + 2CH3COOH(aq)  Casein+(CH3COO)2Ca(aq)

The isoelectric point of casein is 4.7. The purified protein

In addition to being consumed in milk, casein in used in

(ii) Saturated ammonium sulphate solution.

(iii) 1 % acetic acid solution.

2. Take 20 ml of buffalo’s milk in 250 ml beaker and find its weight.

3. Add 20 ml saturated solution of ammonium sulphate slowly with

4. Filter the above solution and transfer the precipitate in another

5. Treat the above precipitate with 30 ml distilled water. Casein

6. Warm the above contents of the beaker to 40 - 45°C on a

7. Filter the precipitated casein and wash with distilled water

8. Find the weight of dry precipitate.

9. Repeat the whole experiment with cow’s milk, goat’s milk

Weight of milk taken = W₁ g

Weight of Casein isolated = W₂ g

Percentage of casein = Weight of Casein x 100

S.no. Type of milk Volume of Weight Weight of Percentage of

1. Buffalo’s 20 23.09 0.632 2.73%

2. Cow’s milk 20 35.66 0.55 1.64%

3. Goat’s milk 20 23.09 0.77 3.67%

Different Samples of milk contains different percentage

Highest percentage of casein is present in Goat’s milk.

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