Department of Microbiology Sem Course Type Course Code Course Title Credits Contact Hours/week CIA Ext Total

DEPARTMENT OF MICROBIOLOGY CLASS: II B.Sc.
Biotechnology
Course Course Contact
Sem Course Title Credits CIA Ext Total
Type Code Hours/week
IV Allied Ancillary 1 2 40
20U4RAP1 60 100
Practical II
Nature of Course
Knowledge and skill
 Employability oriented
Entrepreneurship oriented
Skill oriented
Course Objectives:
1. To familiarize with milk and water quality techniques
2. To know the isolation and identification of nitrogen fixing bacteria from root nodules
3. To explain the methods of enumeration of microbes
4. To identify the bacterial and fungal plant pathogens
5. To explore wine production
Course Learning Outcomes:

On successful completion of the programme, the students will be able to
1. Demonstrate the milk and water quality techniques
2. Isolate and identify nitrogen fixing bacteria from root nodules
3. Experiment with microbial ecology and its interaction
4. Isolate and identify bacteria, fungi and algae
5. Determine the role of bacteria in environment and industrial processes
S.No. Experiments
1. Methylene Blue Reductase Test
2. Resazurin dye reduction test
3. Isolation of Nitrogen fixing bacteria from root nodules of legumes
4. Enumeration of bacteria from soil
5. Enumeration of fungi from soil
Examination of plant diseases – Bacterial diseases of Plants – bacterial blight of rice and citrus
6.
canker
7. Examination of plant diseases –Fungal diseases of Plants –leaf spot of rice and rust of sorghum
8. MPN test
9. Antiobiosis – Plate method using soil
10. Production of wine (demo)
The Academic Council, The Madura College (Autonomous): 17th June 2021 Corrected Copy Page | 1901
Books for Study
1. Aneja, K.R. (2005). Experiments in Microbiology, Plant pathology and Biotechnology. 4th Edition,
New Age International Publishers, Chennai.
2. James G Cappuccino and Natalie Sherman. (2004). Microbiology: A Laboratory Manual. 6th Edition,
Pearson Education, USA.
Books for Reference

1. Ashok, R. (2000). Antimicrobials in Laboratory Medicine, B.I. Churchill Livingstone, New Delhi.
2. Collee, J.G., Fraser, A.G., Marmion B.P. and Simmons. A. (2007). Mackie and McCartney Practical
Medical Microbiology. Elsiever, New York.
3. Ranjan Kumar De, (2007). Diagnostic Microbiology (For DMLT Students). Jaypee Brothers
publishing, New Delhi.
4. Gunasekaran, P. (2008). Laboratory Manual in Microbiology. New Age International (P) Ltd., New
Delhi.
Web Resources
1. https://www.biocourseware.com/iphone/ghistory/index.htm
2. https://www.microbiologynutsandbolts.co.uk/normal-flora.html
3. https://www.microbiologyinfo.com/category/basic-microbiology
Pedagogy
Chalk and talk, PPT, Group discussion, Seminar, Screening of educational videos and quiz
Rationale for nature of the course
Practical skills on various microbiological techniques form the basis of biotechnological experiments.
Isolation, screening, identification and characterization of various types of microorganisms and its
significance in clinical, environmental, industrial processes paved the way for novel biotechnological
inventions. Understanding the nature, growth requirements and characteristics of microorganisms helps
to explore its economic importance in the field of biotechnology.
Activities having direct bearing on skill development/ employability/entrepreneurship

 Providing practical insight on quality of milk and water.
 Characterization of microbes present in environmental samples and exploring its ecological
consequences.
Pedagogy
Demonstration and practical session.
Course Learning Outcomes (CLO)
On completion of this course the students will be able to
Course Learning Outcomes Knowledge

CLOs
Level
On completion of this course the students will be able to
CLO-1 Demonstrate the milk and water quality techniques. Up to K2
CLO-2 Isolate and identify nitrogen fixing bacteria from root nodules. Up to K3
CLO-3 Experiment with microbial ecology and its interaction Up to K3
CLO-4 Isolate and identify bacteria, fungi and algae Up to K1
CLO-5 Determine the role of bacteria in environment and industrial processes. Up to K3
K1 –Remembering and recalling facts with specific answers

K2 – Basic understanding of facts and stating main ideas with general answers
K3 – Application oriented – Solving Problems
K4 – Examining, analyzing, presentation and make interferences with evidences
Mapping of Course Learning Outcome with Programme Specific Outcome
PSO1 PSO2 PSO3 PSO4 PSO5

CLO1 1 2 2 3 2
CLO2 2 2 3 2 2
CLO3 2 3 2 3 2
CLO4 3 3 2 3 2
CLO5 2 3 2 3 2
Advance application–3 Intermediate level –2 Basic level –1
Mapping of course outcome with Programme outcome
PO1 PO2 PO3 PO4 PO5

CLO1 3 2 3 2 3
CLO2 2 3 2 2 2
CLO3 2 2 3 2 3
CLO4 3 3 2 2 2
CLO5 2 2 3 3 3
Advance application – 3, Intermediate level – 2, Basic level – 1.
LESSON PLAN
Experiment
Description Staff Hours Mode
Number
Methylene Blue Reductase Test Practical
1 Add Sample and Dye 3 demo and
Incubate and Observe results learning
Resazurin dye reduction test

Practical
2 Add Sample and Dye
demo
Incubate and Observe results 3
learning
Isolation of Nitrogen fixing bacteria from root
nodules of legumes
3 Group lab
Isolate bacteria from root nodules. 3
work
Identify morphology.
Enumeration of bacteria from soil Joint
4 Isolate soil bacteria productive
3
Enumerate and Identify morphology. activity
Enumeration of fungi from soil

Lecturing
5 Isolate soil fungi
and
Enumerate and Identify morphology. 3
discussion
Examination of plant diseases – Bacterial diseases of

6 Group lab
Plants – bacterial blight of rice and citrus canker
3 work
Examination of plant diseases –Fungal diseases of

7 Group lab
Plants –leaf spot of rice and rust of sorghum
3 work
MPN test.
Prepare media and Sterilize Group lab
8
Inoculate and Incubate 3 work
Observe the results
Group lab
9 Antiobiosis – Plate method using soil 3
work
Group lab
10 Production of wine (demo) 3
work
Total 30 Hrs
Course designers
1. Mr. P. Sasikumar

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DEPARTMENT OF MICROBIOLOGY CLASS: II B.Sc.

Course Learning Outcomes:

Books for Reference

Rationale for nature of the course

Activities having direct bearing on skill development/ employability/entrepreneurship

Demonstration and practical session.

Course Learning Outcomes Knowledge

K1 –Remembering and recalling facts with specific answers

Mapping of Course Learning Outcome with Programme Specific Outcome

PSO1 PSO2 PSO3 PSO4 PSO5

Advance application–3 Intermediate level –2 Basic level –1

Mapping of course outcome with Programme outcome

PO1 PO2 PO3 PO4 PO5

Advance application – 3, Intermediate level – 2, Basic level – 1.

Resazurin dye reduction test

Enumeration of fungi from soil

Examination of plant diseases – Bacterial diseases of

Examination of plant diseases –Fungal diseases of

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