Chemistry

INDIAN CENTRAL SCHOOL
KUWAIT
CHEMISTRY PROJECT:
DETERMINING THE AMOUNT OF CAFFEINE IN
DIFFERENT SAMPLES OF TEA
Done by: Abdur Rahman

Class: 12 – A
Indian Central School Kuwait, 2023-2024
INDIAN CENTRAL SCHOOL
KUWAIT
BONAFIDE CERTIFICATE
This is certified to be the bonafide work done by Abdur

Rahman, registration number _______________Class 12 in
Chemistry during the year, 2023 - 2024.
Internal examiner Head of the department Principal

Teacher’s Code_________
Submitted for Senior Secondary certificate Examination Practical
held at Indian Central School, Kuwait on
_______/_________/20_______.
EXTERNAL EXAMINER
SCHOOL SEAL TEACHER’S CODE_______

Acknowledgement
I express my sincere thanks to Dr. Ayyub Saheb
Shamshuddin my subject Teacher for his guidance
and invaluable suggestions.
Let me thank my school principal Mrs. Sujata

Sivakrishnan for her mentoring and for providing me
with all the facilities in the school for timely project
completion.
I also place on record my gratitude to my classmates
and my parents for their encouragement and
motivation.
DECLARATION
This is to certify that the project work titled

Determining the amount of caffeine in different
samples of tea submitted for the Senior Secondary
Certificate Practical Examination conducted at
INDIAN CENTRAL SCHOOL, Kuwait is a record of my
original work under the guidance of
Dr. Ayyub Saheb Shamshuddin in the year 2023 - 2024
Index
s.no Topic Page.no
1. Introduction 1
2. Materials required 2
3. Theory 3-4
4. Aim 5
5. Uses of caffeine 6
6. Effects of caffeine 7
7. Procedure 8-9
8. Observation 10-11
9. Result 12-13
10. Bibliography 14
Introduction
Tea is the most commonly and widely used
soft beverage in the household. The principle
constituent of tea, which is responsible for all
these properties is the Alkaloid-caffeine. It
acts as a stimulant for central nervous system
and skeletal muscles. It also increases the
capacity of thinking. The amount of caffeine in
tea leaves varies from sample to sample. It
was assumed that caffeine is responsible for
the taste and flavour of tea. But pure caffeine
has been found to be tasteless while
substance. So regardless of caffeine, the
flavour of tea is because of the presence of
some other substance.
1
Materials required
- Different samples of tea leaves

- Beaker
- Burner
- Filter flask
- Lead Acetate
- Chloroform
- Separating funnel
2
Theory
The most important methylated alkaloid that
occurs naturally is caffeine. Its molecular
formula is C8H10N4O2. Its IUPAC name is
1, 3, 7-trimethylxanthene and common name is
1-methylated Thiobromine.
3
Purely it is white, crystalline solid in the form of
needles. Its melting point is 1230c. It is the main
active principle component of tea leaves. It is
present in tea leaves up to 3%. In addition to that,
some people have the habit to drink cups of tea
everyday but can still sleep for long hours. While on
the other hand there are people a small quantity of
consumption of tea might be the reason to dissipate
their sleep for some particular hours of the day.
Therefore, the degree to which an individual is
stimulated by given amount of caffeine differs from
person to person. Caffeine is a xanthine alkaloid,
xanthine is a naturally occurring compound in the
human body and is also found in plant products such
as tea, coffee, and cocoa beans. In fact children are
more susceptible than adults to excitation by
xanthene. A doubt always remains whether to deny
caffeine containing beverages to patients or not.
The xanthene beverages also create a medical
problem. They are dietary of a stimulant of the CNS.
For this reason, tea and coffee should be excluded
from our diet.
4
Aim:
In this project we will study and observe the quantity
of caffeine varying in different samples of tea leaves.
5
Uses of Caffeine:
1. In medicine, it is used to stimulate,

central nervous system and to increase
flow of urine.
2. Because of its stimulating effects,

caffeine had been used to relieve fatigue,
tiredness and headache.
3. Caffeine is also used in analgesic

tablets, as it is believed to be a pain
reliever. It is also beneficial in migraines.
6
Effects of Caffeine:
1. The effects of caffeine can

include increased breathing and heart rate,
and increased mental alertness and physical
energy.
2. On the contrary their excessive use is

harmful to digestion, their long use leads to
mental retardation and one may collapse if
not consumed it under a certain limit.
7
Procedure:
• Take 50 grams of tea leaves of
(1st sample) in a beaker and add 150 ml of
water.
• Place the beaker on a burner and heat it
unit the water starts to boil.
• The solution is now filtered and lead
acetate is added to the filtrate, leading to
the formation of a curdy brown coloured
precipitate.
• Lead acetate is added continuously into
the solution until no more precipitate was
formed.
• Again the solution will be filtered.
• Now the filtrate so obtained was
heated until it becomes 50 ml.
• Let the remaining solution after heating,
cool.
8
• After it has cooled down, put the
solution in a separating flask and add 20 ml
of chloroform to it.
• Soon after, two layers appeared in the
separating funnel.
• We separate the lower layer.
• The solution is then exposed to
atmosphere in order to allow the chloroform
to get evaporated.
 The residue left behind was caffeine.
• Then we weighed it and record the
observations.
Similar procedure was performed with

different samples of tea leaves and quantity
of caffeine was observed in them.
9
Observation:
1. Red Label Tea (Brooke Bond)
Weight of china dish 46.60gms

With precipitate
Amount of caffeine 0.60gms
2. Yellow Label Tea (Lipton)
weight of china dish 46.60gms

With precipitate
10
3. Green Label Tea (Lipton)

weight of china dish 47.05gms
with precipitate
11
Result:
1. Quantity of caffeine in Red label tea is

60mg/sample of 50 gm.
2. Quantity of caffeine in yellow label tea is

3. Quantity of caffeine in green label tea is

.
12
Graphically plotting various tea samples in
accordance with the amount of caffeine
present in them:
Order of quantities of caffeine in different

samples of tealeaves:
Red label >Yellow Label > Green Label
13
BIBLIOGRAPHY
Links
https://www.scrbid.com
https://www.thechemistryguru.com
https://seminarsonly.com
14

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INDIAN CENTRAL SCHOOL

Done by: Abdur Rahman

This is certified to be the bonafide work done by Abdur

Internal examiner Head of the department Principal

SCHOOL SEAL TEACHER’S CODE_______

Let me thank my school principal Mrs. Sujata

This is to certify that the project work titled

- Different samples of tea leaves

1. In medicine, it is used to stimulate,

2. Because of its stimulating effects,

3. Caffeine is also used in analgesic

1. The effects of caffeine can

2. On the contrary their excessive use is

Similar procedure was performed with

Weight of china dish 46.60gms

Weight of china dish 47.20gms

2. Yellow Label Tea (Lipton)

weight of china dish 46.60gms

Weight of china dish 46.60gms

1. Quantity of caffeine in Red label tea is

2. Quantity of caffeine in yellow label tea is

3. Quantity of caffeine in green label tea is

Order of quantities of caffeine in different

Red label >Yellow Label > Green Label

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