Determine Coffine in Tea

All india senior secondary certificate examination 2022-2023
CHEMISTRY PRACTICAL PROJECT REPORT

Determination Of Caffeine In Tea Samples
Delhi Public School
MR Nagar ,Mathura
Submitted For AISSCE(2022-23)
CHEMISTRY
Submitted By Under the

Aryan Sharma supervision of
Mr.Imendra yadav
2
First of all I would like to thank my principal

Mr.salender sing who give me the golden
opportunity to do this wonderful project , I would
also like to thank my Chemistry Teacher Ms.
Imendra yadav in providing a helping hand in
making my project titled “Determination Of
Caffeine In Tea Samples”.
Disclaimers: The errors idiocies and inconsistencies

remain my own.
Aryan Sharma
(Name and signature of the student)
3
It is hereby certify that, the

original and genuine research
work is carried out to investigate
abut the subject matter and the
related data collection and has
been completed solely and
satisfactorily by this student
regarding the Project titled
“Determination Of Caffeine In Tea
Samples”.
Signature
(S ubject
Teacher)
4
CONTENTS
1. Introduction
2. Theory
3. Uses of Caffeine
4. Effects of
Caffeine
5. Procedure
6. Observations
7. Result
A)
INTRODUCTION
Tea is the most commonly and widely used

soft beverage in the household. It acts as a
stimulant for central nervous system and
skeletal muscles. That is why tea removes
fatigue, tiredness and headache. It also
increases the capacity of thinking. It is also
used for lowering body temperature. The
principal constituent of tea, which is
responsible for all these properties, is the
alkaloid-caffeine. The amount of caffeine in
tea leavers varies from sample to sample.
Originally it was thought that caffeine is

responsible for the taste and flavour of tea.
But pure caffeine has been found to be a
tasteless while substance. Therefore, the
taste and flavour of tea is due to some
other substance present in it. There is a
little doubt that the popularity of the
xanthenes beverages depends on their
stimulant action, although most people
are unaware
of any stimulation. The degree to which an
individual is stimulated by given amount
of caffeine varies from individual to
individual.
For example, some people boast their

ability to drink several cups of coffee in
evening and yet sleep like a long, on the
other hand there are people who are so
sensitive to caffeine that even a single cup
of coffee will cause a response boarding on
the toxic.
The xanthene beverages also create a

medical problem. They are dietary of a
stimulant of the CNS. Often the physicians
face the question whether to deny caffeine-
containing beverages to patients or not. In
fact children are more susceptible than
adults to excitation by xanthenes.
For this reason, tea and coffee should be

excluded from their diet. Even cocoa is of
doubtful value. It has a high tannin content
may be as high as 50 mg per cup.
After all our main stress is on the presence

of caffeine in xanthene beverages and so in
this project we will study and observe the
quantity of caffeine varying in different
samples of tea leaves.
8
Theory
The most important methylated alkaloid

that occurs naturally is caffeine. Its
molecular formula is C 8 H 1 0 N 4 O 2 . Its I U P A C
name is 1, 3, 7-trimethylxanthene and
common name is 1-methylated
thiobromine
.
Purely it is white, crystalline solid in the
form of needles. Its melting point is 1230c.
It is the main active principle component
of tea leaves. It is present in tea leaves up
to 3 % and can be extracted by first boiling
the tea leaves with water which dissolves
many glycoside compounds in addition to
caffeine. The clear solution is then treated
with lead acetate to precipitate the
glycoside compounds in the form of lead
complex. The clear filtrate is then extracted
with extracts caffeine because it is more
soluble in it then water.
10
Uses of
Caffeine :
1.In medicine, it is used to stimulate,
central nervous system and to increase
flow of urine.
2.Because of its stimulating effects,
caffeine has been used to relieve
fatigue. But it is dangerous and one
may collapse if not consumes it under
certain limit.
3.Caffeine is also used in analgesic
tablets, as it is believed to be a pain
reliever. It is also beneficial in
migraines.
11
Effects of Caffeine
1.It is psycho - stimulant.
2.It improves physical and mental ability.
3.Its effect in learning is doubtful but

intellectual performance may improve
where it has been used to reduce
fatigue or boredom.
4.When administered internally, it

stimulates heart and nervous system
and also acts as diuretic. On the
contrary their excessive use is harmful
to digestion and their long use leads to
mental retardation.
12
Procedure
 First of all, 50 grams of tea leaves
were taken as sample and 150 ml of
water was added to it in a beaker.
 Then the beaker was heated up to
extreme boiling.
 The solution was filtered and lead
acetate was added to the filtrater,
leading to the formation of a curdy
brown coloured precipitate.
 We kept on adding lead acetate till no
more precipitate has been formed.
 Again solution was filtered.
 N ow the filtrateso obtained was
heated until it had become 50 ml.
 Then the solution left was allowed to
cool.
 A fter that, 20 ml. of chloroform
was added to it.
 Soon after, two layers appeared in the
separating funnel.
 W e separated the lower
layer.The solution then exposed to
 atmosphere in order to
allow
chloroform to get evaporated.
 The residue left behind was caffeine.
 Then we weighed it and
recorded the observations.
Similar procedure was performed with

different samples of tealeaves and quantity
of caffeine was observed in them
1.Red Label Tea (Brooke Bond)
Weight of china dish 46.60gms

Weight of china dish 47.20gms.
with precipitate
Amount of caffeine 0.60gms
2.Yellow Label Tea (Lipton)
Weight of china dish 46.60gms

with precipitate
Amount of caffeine 0.55gms
3.Green Label Tea (Lipton)

with precipitate
Amount of caffeine 0.45gms.
1. Quantity of caffeine in Red la6el tea is
60mg. /sample of Z0 gm.
2. Quantity of caffeine in yellow

la6el tea is ZZmg./sample of Z0
gm.
3. Quantity of caffeine in green la6el tea is

4Zmg./sample of Z0 gm.
16
Graphically plotting various tea samples in

accordance with the amount of caffeine
present in them we present a stunning find:
0.6
0.5
0.4
0.3
0.2
0.1
0
60 mg 55 mg 45 mg
RED LABEL YELLOW LABEL GREEN

LABEL
Order of quantities of caffeine

in different samples of tealeaves

Determine Coffine in Tea

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All india senior secondary certificate examination 2022-2023

CHEMISTRY PRACTICAL PROJECT REPORT

Submitted By Under the

First of all I would like to thank my principal

Disclaimers: The errors idiocies and inconsistencies

It is hereby certify that, the

Tea is the most commonly and widely used

Originally it was thought that caffeine is

For example, some people boast their

The xanthene beverages also create a

For this reason, tea and coffee should be

After all our main stress is on the presence

The most important methylated alkaloid

2.It improves physical and mental ability.

3.Its effect in learning is doubtful but

4.When administered internally, it

Similar procedure was performed with

Weight of china dish 46.60gms

2.Yellow Label Tea (Lipton)

Weight of china dish 46.60gms

3.Green Label Tea (Lipton)

Weight of china dish 46.60gms.

2. Quantity of caffeine in yellow

3. Quantity of caffeine in green la6el tea is

Graphically plotting various tea samples in

RED LABEL YELLOW LABEL GREEN

Order of quantities of caffeine

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