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1 Yeast cells make the enzyme sucrase. Sucrase catalyses the breakdown of sucrose to glucose
and fructose.

Some students made an extract of sucrase from yeast cells.

They investigated the activity of the sucrase extract at different pH values. They determined the
rate of reaction at each pH.

They then calculated the rate of each reaction as a percentage of the fastest reaction, to give the
percentage activity of sucrase.

The results of this investigation are shown in the graph.

Describe and explain the effect of pH on the activity of sucrase shown in the graph.

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[Total: 6]

2 Yeast cells make the enzyme sucrase. Sucrase catalyses the breakdown of sucrose to glucose
and fructose.

Enzymes are made of protein.

Explain how the shape of a sucrase molecule is related to its function.

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[Total: 2]
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3 The photograph shows a fruit attached to the branch of an orange tree, Citrus sinensi.

Biotechnologists use enzymes to extract juice from fruit such as oranges.

Define the term enzyme.

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[Total: 2]
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4 Alcohol dehydrogenase is an enzyme that breaks down alcohol in the body.

The graph shows the activity of alcohol dehydrogenase at different pH values.

Suggest one other factor that could affect the activity of the enzyme alcohol dehydrogenase.

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[Total: 1]
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5 Alcohol dehydrogenase is an enzyme that breaks down alcohol in the body.

The graph shows the activity of alcohol dehydrogenase at different pH values.

State the pH value with the highest enzyme activity in the graph.

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[Total: 1]
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6 Alcohol dehydrogenase is an enzyme that breaks down alcohol in the body.

The graph shows the activity of alcohol dehydrogenase at different pH values.

State the pH value with the lowest enzyme activity in the graph.

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[Total: 1]

7 Enzymes are biological catalysts.

Define the term catalyst.

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[Total: 2]
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8 Pectinase is an enzyme that is used in the production of apple juice.

A student investigated how pH affected the volume of apple juice produced when using pectinase.

The student chopped an apple into small pieces.

The pieces of apple were put into solutions with different pH values.

Pectinase was added to each solution.

After two hours the mixture was filtered and the volume of apple juice obtained was recorded.

The table shows the results.

volume of apple
pH 3
juice obtained / cm

1.0 23.2

2.0 24.2

3.0 23.5

4.0 25.7

5.0 27.6

6.0 27.4

7.0 24.0

8.0 22.0

State one factor, other than pH, that would affect the activity of pectinase.

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[Total: 1]
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9 Pectinase is an enzyme.

The box on the left shows the beginning of a sentence.

The boxes on the right show some endings of sentences.

Draw three lines from the word ‘Enzymes’ to make three correct sentences.

are living organisms.

are proteins.

can only be used once.

Enzymes

have a complementary shape to their substrate.

increase the rate of chemical reactions.

in the stomach are most active at pH 8.

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[Total: 3]

10 Amino acids are transported in plants.

State the name of the group of molecules that are made of proteins and act as catalysts.

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[Total: 1]