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Osgu Shs BMLT

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SCHOOL

OF

Health Sciences

(SHS)

Bachelor of Health Science

Medical Lab Technology (MLT)

Duration – 4 Years (8 Semesters)

Maximum Duration – 8 Years

Curriculum Structure
(Academic Session 2020-21 Onwards)
Om Sterling Global University
Bachelor of Sciences Medical Lab Technology

PROGRAM OBJECTIVES

The objective of B.Sc. (Medical Lab Technology) program for students keen to gain expertise in
procedures for diagnostic testing in a laboratory. The course helps them analyze and identify
substances, and microorganisms using common instruments found in a laboratory. Students are
given in-depth knowledge of Chemistry, Human Biology, Organic Chemistry, Haematology,
Bacteriology and Medical Technology and practical sessions in laboratory procedures, analysing
various substances including glucose, enzymes, electrolytes, spectrometer, laboratory
mathematics, and clinical applications.

LEARNING OUTCOMES

 Apply knowledge and technical skills associated with medical laboratory technology for
delivering quality clinical investigations support.
 Perform routine clinical laboratory procedures within acceptable quality control
parameters in hematology, biochemistry, immunohematology and microbiology.
 Demonstrate technical skills, social behavior and professional awareness for functioning
effectively as a laboratory technician.
 Apply problem solving techniques in identification and correction of pre analytical, post
analytical & analytical variables.
 Operate and maintain laboratory equipments utilizing appropriate quality control and
safety procedures.
 Recognize the impact of laboratory tests in a global and environmental context.
 Communicate effectively by oral, written and graphical means.
 Function as a leader / team member in diverse professional and industrial research areas.
 Apply the fundamentals of research process to complete and present research studies that
enrich the field of physical therapy.
 Function in an ethical and professional manner without bias against any ethnicity, race, ,
religion, caste or gender.
 Practice professional and ethical responsibilities with high degree of credibility, integrity
and social concern.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwards Page 2
Om Sterling Global University
Common Instructions and Guideline for all semesters and subjects
1 Question Paper Pattern
(i) Where the End Term Examination is of Maximum 60 Marks
Section A: MCQ/Fill in blanks/Short answer questions (up to 25 words) 5 x2 marks = 10
marks. All five questions are compulsory.
Section B: Analytical/Problem Solving questions (up to 100 words) 4 x 5 marks = 20
marks. Candidate has to answer four questions out of five.
Section C: Descriptive/ Analytical/Problem solving question 2 x 15 marks = 30 marks.
Candidates have to answer two questions out of three.
(ii) Where the End Term Examination is of Maximum 30 Marks
Section A: MCQ/Fill in blanks/Short answer questions (up to 25 words) 5 x1 marks = 5
marks. All five questions are compulsory.
Section B: Analytical/Problem Solving questions (up to 100 words) 4 x 2.5 marks = 10
marks. Candidate has to answer four questions out of five.
Section C: Descriptive/ Analytical/Problem solving question 2 x 7.5 marks = 15 marks.
Candidates have to answer two questions out of three

2. Continuous Assessment: All courses undertaken by students are evaluated during the
semester using internal system of continuous assessment. The students are evaluated on
class /tutorial participation, assignment work, lab work, class tests, mid-term tests, quizzes
and end semester examinations, which contribute to the final grade awarded for the subject.
Students will be notified at the commencement of each courses about the evaluation
methods being used for the courses and weightages given to the different assignments and
evaluated activities. Here marks obtained in the internal assessment and end semester
examination are added together and a 10-point grading system will be used to award the
student with on overall letter grade for the course (subject).
Distribution of Mark

(i) Courses without -Practical Components


(a) Attendance Class participat ion - 05 Marks
(b) Assignment s, Class Tests, Quizzes, Projects, Seminar etc. - 15Marks 

  40 Marks
(c) Midterm Test I - 10 Marks 
(d) Midterm Test II - 10Marks 

(e) End –Term Examination - } 60 Marks


__________________________________________

Total 100 Marks

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwards Page 3
Om Sterling Global University
(ii)Courses with -Practical Components only

Internal -Practical Examination and Continuous Progress- 50


End –Term Examination (-Practical) - 50

Total : 100

Grade distribution in a course/Subject


To assess the performance of a student the grading & grade point system as given below in the
table.

Sr. % of Marks Performance Letter Grade Grade Point


No.

1 ≥ 90% & ≤100% Outstanding O 10

2 ≥ 80% & <90% Excellent A+ 9

3 ≥ 70% & <80% Very Good A 8

4 ≥ 60% & <70% Good B+ 7

5 ≥ 50% & <60% Above B 6


Average

6 ≥ 45% & <50% Average C 5

7 ≥ 40% & <45% Pass D 4

8 < 40% Fail F 0

9 Absent Fail Ab 0

1. A student has to obtain minimum ‘D’ grade in theory & -Practical to pass in a
course/subject and to earn credit for that subject.
2. For award the Grade in a course/subject, marks obtained in Internal Assessment and
External Assessment are added together provided the student has obtained a minimum of
40% marks in Internal Assessment as well as External Assessment separately and
Combined Grade is awarded in the course/subject as per grading system given above. In
case a student fails to secure a minimum of 40% marks in Internal Assessment or
External Assessment or in both Internal Assessment and External Assessment, the
Combined Grade awarded is Grade F (Fail).

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwards Page 4
Om Sterling Global University
3. Student has to reappear in the external examination or internal examination or both of
theory & -Practical, if he/she has been awarded ‘F’ grade or Absent (Ab) in any
course/subject.
4. For non credit course only Satisfactory or Unsatisfactory shall be indicated instead of
letter grade and this will not be counted for computation of SGPA or CGPA.
5. Semester Grade Point Average (SGPA)
SGPA for a semester is calculated as under, after the student has passed in all the
courses/subjects of a semester.
k k
SGPA    Credits of Subject i  Grade Points for Subject i    Credits of Subject i 
i 1 i 1

Where k = No. of Courses/Subjects in Semester.


6. Cumulative Grade Point Average (CGPA)
CGPA is calculated as under, after the student has passed in all the courses/subjects of
all the semesters of the program as per prescribed scheme of studies.
N N
CGPA    Credits of Semester j  SGPA of Semester j    Total Credits of Semester j 
j 1 j 1

Where N = Total Number of Semester in the Program.


7. Conversion of Grade Point Average Into Percentage of Marks
For conversion of Grade point Value of CGPA for all the semester, into percentage of
marks; the CGPA is to be multiplied by 10
(% Marks= 10 X CGPA).
8. Award of Division (Course/Subject to earning of total prescribed credit of all the
semester):
1 7.5 ≤ CGPA I Division with Distinction
2 6 ≤ CGPA < 7.5 I Division
3 5 ≤ CGPA < 6 II Division
4 4.5 ≤ CGPA < 5 Pass
Calculation of SGPA and CGPA:

Example:

Table 2

Courses Credits Letter Grade Credit Grade


Grade Value Value Points

A 3 B+ 7 3x7 21

B 3 A 8 3x8 24

C 3 A+ 9 3x9 27

D 2 A 8 2x8 16

TOTAL 11 88

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwards Page 5
Om Sterling Global University
In this case SGPA= Total Grade Points 88 = 8.0

Credits 11

Suppose the SGPA in two successive semesters are 7.0 and 8.0 with 26 and 24 respective
course credits, then the

CGPA = 7x26+8x24 = 374 = 7.48


26+24 50

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwards Page 6
Om Sterling Global University
B.Sc. Medical Lab Technology

3rd Semester

Sr. Teaching
Course Total Evaluation Scheme Exam.
No Course Title Schedule Credit
Code Hours Hours
. L T P Internal External Total
Pathology-I BHMT-301 4 0 0 4 4 40 60 100
1. 3
Clinical BHMT-302 4 0 0 4 4 40 60 100
2. 3
Hematology-I
Microbiology, BHMT-303 4 0 0 4 4 40 60 100
3. Immunology & 3
Serology-I
Histopathology & BHMT-304 4 0 0 4 4 40 60 100
4. 3
Histotechniques -I
Clinical BHMT-321 0 0 2 2 1 50 50 100
5. Hematology-I- 3
Practical
Microbiology, BHMT-322 0 0 2 2 1 50 50 100
Immunology &
6. 3
Serology – I-
Practical
Histopathology & BHMT-323 0 0 2 2 1 50 50 100

7.
Histotechniques - 3
I- Practical

Comprehensive BHMT-324 0 0 2 2 1 50 50 100


8. Viva 3

Total 16 0 8 24 20 360 440 800

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwards Page 7
Om Sterling Global University
B.Sc. Medical Lab Technology

4th Semester

Teaching Total
Sr. Evaluation Scheme Exam.
Course Title Course Code Schedule Hour Credit
No. Hours
L T P s Internal External Total
Pathology-II BHMT-401 4 0 0 4 4 40 60 100
1. 3
Clinical BHMT-402
2. 4 0 0 4 4 40 60 100 3
Hematology-II
Microbiology, BHMT-403 4 0 0 4 4 40 60 100
3. Immunology & 3
Serology-II
Histopathology & BHMT-404 4 0 0 4 4 40 60 100
4. 3
Histotechniques -II
Clinical BHMT-421
0 0 2 2 1 50 50 100
5. Hematology-II 3
-Practical
Microbiology, BHMT-422 0 0 2 2 1 50 50 100
Immunology &
6. 3
Serology - II -
Practical
Histopathology & BHMT-423 0 0 2 2 1 50 50 100
7. Histotechniques -II 3
-Practical
Comprehensive BHMT-424 0 0 2 2 1 50 50 100
8. 3
Viva
Total 16 0 8 24 20 360 440 800

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwards Page 8
Om Sterling Global University
B.Sc. Medical Lab Technology

5th Semester

Teaching
Sr. Total Evaluation Scheme Exam.
Course Title Course Code Schedule Credit
No. Hours Hours
L T P Internal External Total
Immunohematology BHMT-501 4 0 0 4 4 40 60 100
1. & 3
Blood Banking
Clinical BHMT-502 4 0 0 4 4 40 60 100
2. Enzymology & 3
Automation
Parasitology & BHMT-503 4 0 0 4 4 40 60 100
3. Virology 3

Diagnostic BHMT -504 4 0 0 4 4 40 60 100


4 3
Cytology
Principles of BHMT-505 4 0 0 4 4 40 60 100
Laboratory
5 3
Management &
Medical Ethics
Clinical BHMT-521 0 0 2 2 1 50 50 100
Enzymology &
6 3
Automation -
Practical
Parasitology & BHMT-522 0 0 2 2 1 50 50 100
7 Virology 3
-Practical
Diagnostic BHMT-523 0 0 2 2 1 50 50 100
8. Cytology 3
-Practical
Total 20 0 6 26 23 350 450 800

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwards Page 9
Om Sterling Global University
B.Sc. Medical Lab Technology

6th Semester

Teaching
Sr. Total Evaluation Scheme Exam.
Course Title Course Code Schedule Credit
No. Hours Hours
L T P Internal External Total
Clinical BHMT-601 4 0 0 4 4 40 60 100
1. Endocrinology & 3
Toxicology
Advanced BHMT-602 3 0 0 3 3 40 60 100
2. Diagnostic 3
Techniques
Diagnostic BHMT-603 3 0 0 3 3 40 60 100
3. Molecular 3
Biology
Medical Pathology BHMT-604 3 0 0 3 3 40 60 100
4. 3
Research BHRM-651 3 0 0 3 3 40 60 100
5. Methodology & 3
Biostatistics
Clinical BHMT-621 0 0 2 2 1 50 50 100
Endocrinology &
6. 3
Toxicology -
Practical
Advanced BHMT-622 0 0 2 2 1 50 50 100
Diagnostic
7. 3
Techniques -
Practical
Diagnostic BHMT-623 0 0 2 2 1 50 50 100
8. Molecular 3
Biology -Practical
Clinical Posting BHMT-624 0 0 4 4 2 50 50 100
9. (Orientation) 3

Project Work -I BHMT-625 0 0 4 4 2 50 50 100


10. 3
Total 16 0 14 30 23 450 550 1000

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 10


Om Sterling Global University
B.Sc. Medical Lab Technology

7th Semester

Teaching
Sr. Course Evaluation Scheme
Schedule Total Credit Exam
Course Title
No. Code Hours Hours
L T P Internal External Total
Internship BHMT-721 0 0 24 24 12 300 300 600
1. 3
Project Work-II BHMT-722 0 0 12 12 6 150 150 300
2. 3

Total 0 0 36 36 18 450 450 900

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 11


Om Sterling Global University
For International Students
B.Sc. Medical Lab Technology

8th Semester

Teaching
Sr. Course Total Evaluation Scheme Exam
Course Title Schedule Credit
No. Code Hours Hours
L T P Internal External Total
Special BHMT-821 0 0 24 24 12 300 300 600
Internship
1. with 3
Research
Project
0 0 24 24 12 300 300 600
Total

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 12


Om Sterling Global University
B.Sc. Medical Lab Technology
Third Semester

BHMT-301: Pathology -I
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Introduction & History of pathology, Basic definitions and familiarization with the common
terms used in pathology, Causes and mechanisms of cell injury, reversible and irreversible
injury, Introduction of hyperplasia, hypoplasia, hypertrophy, atrophy, metaplasia, necrosis and
apoptosis

Unit-II
General features of acute and chronic inflammation: Vascular changes, cellular events, Cells
and mediators of inflammation, Phagocytosis and its mechanism

Unit-III
Tissue Renewal and Repair, healing and fibrosis, cirrhosis, introduction of oedema,
hyperaemia, congestion, haemorrhage, haemostasis, thrombosis, embolism, infarction, shock
and hypertension.

Unit-IV
Protein energy malnutrition, deficiency diseases of vitamins and minerals, nutritional excess
and imbalances. Role and effect of metals (Zinc, Iron and Calcium) and their deficiency
diseases, Aetiology and pathophysiology of diabetes, arteriosclerosis, myocardial infarction,
respiratory diseases (COPD), Parkinson disease
Infectious Diseases: pathogenesis & overview of modes of infections, prevention and control
with suitable examples like Typhoid, Dengue
Cancer: Definitions, nomenclature, characteristics of benign and malignant neoplasm,
metastasis, Carcinogens and cancer, concept of oncogenes, tumour suppressor genes, DNA
repair genes and cancers stem cells.

Text & Reference Books:


1.Harshmohan (2017), Textbook of Pathology,7th edition, Jaypee Publications
2.Robbins,(2012), Text book of Pathology, 3rd edition, Elsevier Publication

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 13


Om Sterling Global University
BHMT-302: Clinical Hematology -I
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
.RBCs formation, morphology, cytoskeleton, anisocytosis, poikilocytosis, metabolism and
its classification, Morphological and etiological, pathogenesis, laboratory investigations and
management. Iron deficiency anaemia, metabolism of iron, pathogenesis, laboratory
investigations and management, principle and procedure of special test .Megaloblastic
anaemia, pernicious anaemia, pathogenesis, laboratory investigations

Unit-II
Haemoglobin, its synthesis and types, normal and abnormal hemoglobins, extravasccular and
intravascular hemolysis.Haemolytic anaemia, pathogenesis and laboratory investigations,
principle and procedure of special test, G-6-PD

Unit-III
Leukopoiesis , Stages of Leukocyte Maturation, Features of Cell Identification, leucocytosis
and leucocytopenia , neutrophilia , eosinophilia, basophilia, monocytosis, lymphocytosis,
neutropenia, lymphopenia, causes and significance, toxic granulation, Morphological
alterations in neutrophil, effect of HIV on blood cell parameter

Unit-IV
Overview of hemostasis and coagulation, Stages of platelets development, Primary and
Secondary hemostasis, Role of platelets, Role of coagulation factors, Coagulation inhibitory
system, Fibrinolysis .General blood picture, estimation of iron, TIBC, Transferrin, Ferritin,
Plasma haemoglobin,Vit.B12, Folic acid, FIGLU test, Schiling test, Parietal cell antibodies,G-
6-PD, Osmotic fragility test, Heinz bodies, Perls Prussian staining, Platelet count, Platelet
aggregation test, PT, INR APTT, Mixing experiments in PT and APTT, Thrombin time.

Text & Reference Books:


1.Mukherjee .L.K(2017), Medical Laboratory Technology,Vol.1-3,3rd edition, Tata Mcgraw
Hill

2.Sood Ramnik,(2015), Text book of Medical Laboratory Technology,2nd edition, Jaypee


Publication

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 14


Om Sterling Global University
BHMT-303: Microbiology, Immunology & Serology -I
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit -I
Introduction to bacterial taxonomy, Classification of Bacteria, Morphology based on size,
shape, arrangement, motility, flagella, spores, capsules, cell wall, plasma membrane, pili,
ribosomes. Microscopy: Study of compound microscope – magnification, numerical
aperture, resolution and components of microscope. Dark ground illumination, care of
microscope and common difficulties micrometry. Bright Field Microscope, Dark Field
Microscope, Phase Contrast Microscope, Fluorescence Microscope, Transmission Electron
Microscope, Scanning Electron Microscope

Unit-II
Cell size, shape and arrangement, cell-wall, composition and detailed structure of Gram-
positive and Gram-negative cell walls, Cell Membrane: Structure, function and chemical
composition of bacterial cell membranes. Cytoplasm: Ribosome, mesosomes, inclusion
bodies, nucleoid, chromosome and plasmids, Endospore: Structure, formation

General safety measures used in Microbiology laboratory, Sterilization and disinfection:


Various physical methods of sterilization – heat, UV radiation, ionizing radiation, filtration,
characters affecting sterilization, auto clave control and sterilization indicators.

Biomedical waste management in a Medical Microbiology laboratory: Types of the waste


generated, Segregation, Treatment, Disposal

Antiseptics & Disinfectants: Definition, types and properties, mode of action, use, qualities
of good disinfectants. Chemical disinfectants – phenol and its compounds, alcohol, halogen,
heavy metals and quaternary ammonium compounds, aldehyde, gaseous compound. use and
abuse of disinfectants. precautions while using the disinfectants.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 15


Om Sterling Global University
Unit-III
Antigens and haptens:Properties ,foreignness, molecular size, heterogeneity, B and T cell
epitopes; T dependent and T independent antigens.

Antibodies: Historical perspective of antibody structure; structure, function and properties of


the antibodies; different classes, subclasses and biological activities of antibodies; concepts
of antibody diversity, isotype, allotype, Introduction of hybridoma technology, monoclonal
antibodies, polyclonal antibody.
Mechanism of humoral and cell mediated immune response.
Introduction of Major Histocompatibility Complex, organization of MHC and inheritance in
humans; Antigen presenting cells, antigen processing and presentation Complement system
and complement fixation test.

Unit-IV
Laboratory tests for demonstration of antigen – antibody reaction such as agglutination,
precipitation, ELISA, RIA, Immunofluorescence, Rheumatological diseases, etiology and
pathogenesis and lab investigations

Text & Reference Books:


1.Abbas AK, Lichtman AH, Pillai S. (2007). Cellular and Molecular Immunology. 6th
edition Saunders Publication, Philadelphia.

2.Delves P, Martin S, Burton D, Roitt IM. (2006). Roitt’s Essential Immunology.11th


edition Wiley- Blackwell Scientific Publication, Oxford.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 16


Om Sterling Global University
BHMT-304: Histopathology & Histotechniques-I
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Introduction of histopathology, cytology & histotechniques, laboratory organization, care &
maintenance of equipments used in histotechnology lab ,Safety measures in histotechnology
lab Reception, Recording, Labelling and transportation of tissue specimens, Basic concepts
of fixation and various types of fixative used in histopathology and cytopathology

Unit-II
Tissue and its types, Location and function, Grossing of tissues, whole mount, sections,
smears, tissue processing and its steps, manual and automated method, components &
principle of automatic tissue processor .Decalcification, decalcification methods, types of
decalcifying fluid, Processing of bones and teeth, Embedding media, its type and properties

Unit-III
Microtome, its type and working, various type of microtome, Microtome knives, its type
and knife sharpening, Section cutting, fault and remedies, Section adhesive Cryostat, frozen
sections of fresh, fixed and unfixed tissue, freeze drying, rapid frozen sections and staining
for emergency diagnosis

Unit-IV
Dye chemistry, Stains and dyes, natural dye, acidic dye, basic dye, neutral dyes, fluorescence
dye, mordant, accelerators, accentuators, metachromasia, metachromatic dyes
Progressive, regressive, vital, supravital staining, types of hematoxylin, Haematoxylin and
eosin staining, use of control sections in tissue staining, mounting and mounting media,
advantages & disadvantages, refractive index
Text & Reference Books:
1.Bancroft's Theory and Practice of Histological Techniques, 7th Edition, Elsevier
Publications
2.Harshmohan (2017), Textbook of Pathology,7th edition, Jaypee Publications

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 17


Om Sterling Global University
BHMT-321: Clinical Hematology -I -Practical

Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1. Determination of haemoglobin by various methods.


2. Determination of Total RBC count.
3. Determination of PCV
4. Determination of red cell indices
5. Demonstration of hypochromic microcytic slide.
6. General blood picture
7. Determination of G-6-PD
8. Differential Leucocute Count.
9. Absolute leucocyte count
10. Demonstration of toxic granulation of neutrophil
11. To perform PT and Calculate INR
12. To perform APTT
13. To perform sickling test
14. Determination of Plasma Hemoglobin
15.To perform reticulocyte count

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 18


Om Sterling Global University
BHMT-322: Microbiology, Immunology & Serology-I -Practical

Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1. Demonstration of Microscope and its parts


2. Demonstration of glassware used in microbiology.
3. Demonstration of autoclave and sterilization of glass wares.
4. Demonstration of Hot air oven and sterilization of glass wares.
5. To perform Gram staining
6. To perform Acid fast staining (Zeihl Neelsen staining)
7. To perform Indian ink staining
8. To perform Hanging drop method
9. Demonstration of capsule
10. Staining of bacterial spores
11. To demonstrate agglutination reaction.
12. To perform RA test
13. To perform WIDAL test
14. To perform RPR test.
15. To perform CRP test.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 19


Om Sterling Global University
BHMT-323: Histopathology & Histotechniques -I -Practical
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1. Demonstration of glass wares and equipment used in histopathology lab.


2. To prepare alcohol of different concentration.
3. To prepare formalin from stock solution.
4. To sharp knife by honing and stropping.
5. Grossing of tissue
6. To perform tissue processing by manual method.
7. To perform section cutting of paraffin embedded tissue.
8. To fix the smear on glass slide.
9. To perform hematoxylin and eosin staining.
10. Mounting and preservation of slide.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 20


Om Sterling Global University
BHMT-324: Comprehensive Viva
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

Comprehensive oral examination of theory subjects of third semester.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 21


Om Sterling Global University
B.Sc. Medical Lab Technology

Fourth Semester

BHMT-401: Pathology -II


Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Hemoglobin, structure ,function and types , Hemoglobinometry , Haemoglobin estimation by
various methods, advantages and disadvantages, physiological and pathological variations on
blood parameters, Hemocytometry, visual and electronic method, neubauer counting chamber.

Unit-II
RBC count, WBC count, Platelets count, absolute eosinophil count, principle, procedure,
calculation , significance, precautions involved during counting, absolute count of various
WBCs. Physiological and pathological changes in values Erythrocyte sedimentation rate,
manual and automated method, factor affecting ESR, packed cell volume, red cell indices
(MCV, MCH, MCHC), Physiological and pathological variations in value
Complete blood count, determination by automated method and significance of each
parameter, Reticulocyte count, routine examination of CSF, semen, sputum and stool.

Unit-III
Mechanism of coagulation, coagulation factors, Bleeding time, clotting time, platelet count,
protamine sulphate test, clot retraction test

Unit-IV
Introduction to immuno hematology and blood banking technology, antigen, antibody,
complements,ABO & Rh blood group system, method of determination, other blood group
system, Donor selection, blood collection, anticoagulants, additive systems, blood bags, its
labelling , storage and transportation
Text & Reference Books:
1. Godkar.B. Praful,(2016) Textbook of MLT,3rd edition,Bhalani Publications

2. Singh Tejinder,(2014),Atlas & Textbook of Haematology,3rd edition, Avichal Publications


3. Ochei J & Kolhatkar A(2000),Medical Laboratory Science: Theory & Practice, 3rd
edition,Mcgraw Hill Education

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 22


Om Sterling Global University
BHMT-402: Clinical Hematology -II
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Aplastic anaemia, Anaemia of chronic disorders, Sideroblastic anaemia, Haemolytic Anaemia,
etiology, pathogenesis, clinical features, laboratory investigations, Bone marrow examination,
composition & functions, aspiration techniques, processing and staining

Unit-II
Hemoglobinopathies, qualitative and quantitative Sickle cell anaemia, sickle cell trait,etiology,
pathogenesis, clinical features, and laboratory investigations, Disease management and prognosis,
Sickling test Thalassaemia, classification, etiology, pathogenesis, clinical features, laboratory
investigations, haemoglobin electrophoresis

Unit-III
Leukemia and its classification, WHO and FAB classification, AML, ALL, CML, CLL, its
etiology, clinical features, laboratory investigations Cytochemistry involved in diagnosis of
various types of leukemia.

Unit-IV
Qualitative and quantitative disorders of platelets, hypercoaguable test, Disorders of secondary
hemostasis, hemophilia and its lab diagnosis, Von-Willebrand disease, Disseminated intravascular
coagulation, thrombosis, Disorder of fibrinogen, test for bleeding & coagulation disorders,
correction studies for factor deficiency, quantitative factor assay

Text & Reference Books:


1. Wintrobe’s Clinical Hematology,(2014),13th edition, Lippincott Williams & Wilkins
2. De Gruchy’s Clinical Hematology in Medical Practice,(2012),Sixth edition, Wiley
Publications
3. Dacie & Lewis -Practical hematology, (2011),11th edition, Elsevier Publication.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 23


Om Sterling Global University
BHMT-403: Microbiology, Immunology & Serology -II
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)
4L+0T+0P End Term Exam.: 3 Hours
Unit-I
Lab organization, management, recording of results and quality control in Medical Microbiology
Lab. Safety measures in Microbiology Laboratory, Occurrence of lab infections, route of
infections in laboratory, safety measures precaution in use of pathogens in teaching.
Host pathogen interaction: Definitions - Infection, Invasion, Pathogen, Pathogenicity, Virulence,
Toxigenicity, Carriers and their types, Opportunistic infections, Nosocomial infections.
Transmission of infection

Unit-II
Principle, working, use, care & maintenance of Laminar air flow, Centrifuge, Autoclave, hot air
Oven, Incubator, Colony Counter, Muffle Furnace, Mac-intos Field-jar etc. Sterility testing of I/v
fluids, Collection, transportation and processing of I/v fluids for bacterial contamination,
Recording the result and interpretation
Hospital acquired infection, Specimen collection from patients, clinics and hospitals, Specimen
collection for epidemiological investigations, role of microbiology laboratory in control of
Nosocomial infection Antimicrobial agents and Antibiotics: Introduction, mechanism of action,
classification and uses, Antibiotic susceptibility testing in bacteriology, Culture medium used for
Antibiotic susceptibility testing, Preparation and standardization of inoculums, Control bacterial
strains, Description, morphology, cultural characteristics, pathogenecity, cultural characteristics,
clinical features and lab diagnosis of Staphylococcus, Streptococcus, Pneumococcus, Neisseria,
Bordetella

Unit-III
Western blotting, Immunodiffusion, Immunoelectrophoresis, Hypersensitivity and its types
Introduction to Allergy and its laboratory test
Introduction of transplant immunology, graft rejection, tissue typing for kidney and bone marrow
transplant, Laboratory test for transplant.
Unit-IV
Autoimmune disorders, pathogenesis, organ specific and systemic autoimmune disorders and its
markers such parietal cell antibody, anti sperm antibody, lupus anticoagulants, anti mitochondrial
antibody, ANA, DNA, HLA-B27, ASMA, anti CCP .
Immunological disorders: primary and secondary immunodeficiency, SCID, AIDS, Tumour,
types of tumours, Various Tumour Markers, their significance and method of estimation.
Text & Reference Books:
1.Abbas AK, Lichtman AH, Pillai S. (2007). Cellular and Molecular Immunology. 6th edition
Saunders Publication, Philadelphia.
2.Ananthanarayan R. and Paniker C.K.J. (2009) Textbook of Microbiology. 8th edition,
University Press Publication

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 24


Om Sterling Global University
BHMT-404: Histopathology & Histotechniques-II
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Staining of carbohydrates: preparation of Schiff reagent, PAS staining, Alcian blue, staining of
glycogen, Amyloid, other staining method Connective tissue & its staining: Trichrome staining,
verhoeff stain, Weigert Resorcin stain, Gordon’s and Sweet stain, Gomori’s method, von
Geison stain, PTAH stain

Unit-II
Demonstration of minerals and pigments in tissue sample, Demonstration and identification of
lipids, Demonstration of enzymes, diagnostic application and the demonstration of phosphatases,
dehydrogenases, oxidases and peroxidases, Demonstration of microorganism on tissue
specimens, Bacteria, AFB, Actinomyces, spirochetes, fungi

Unit-III
Demonstration of nucleic acids, Processing and staining of bone marrow sample. Fixation,
Processing and section cutting of bones, eye ball, Techniques in neuropathology: Neurons
staining, Myelin, Neuropathology lab specimen handling

Unit-IV
Demonstration of sex chromatin, Museum techniques Electron microscopy: Principle and
working, fixation, processing and staining of tissue Fluorescence Microscope: Principle and
working

Text & Reference Books:


1.Bancroft's Theory and Practice of Histological Techniques, 7th Edition, Elsevier Publications
2. Harshmohan (2017), Textbook of Pathology,7th edition, Jaypee Publications
3. Godkar.B. Praful,(2016) Textbook of MLT,3rd edition,Bhalani Publications
4. C F A Culling,(1974),Handbook of Histopathological and Histochemical Techniques:
Including Museum Techniques,3rd edition, Butterworths Publisher

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 25


Om Sterling Global University
BHMT-421: Clinical Hematology- II -Practical

Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1. Staining of bone marrow


2. To perform sickling test.
3. To determine fetal haemoglobin
4. To perform Heinz bodies
5. Demonstration of leukemic slides
6. To perform LAP scoring
7. To determine total platelet count
8. To perform PT
9. To perform APTT
10. To perform thrombin time.
11. To perform D-dimer test.
12. To determine fibrinogen conc.
13. General blood Picture
14. To demonstrate malarial slide
15. Haemoglobin electrophoresis

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 26


Om Sterling Global University
BHMT-422: Microbiology, Immunology & Serology -II -Practical
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1.Demonstration of Autoclave and sterilization of media


2. Demonstration of Laminar air flow and media preparation
3. Preparation of culture plates
4. Demonstration of Centrifuge.
5. Demonstration of hot air Oven and sterilization of glassware’s
6. Demonstration of Incubator and preservation of cultures
7. Preparation of media
8. Antibiotic sensitivity test.
9. Microscopic examination of urine
10. Examination of urine
11. Examination of sputum
12. To perform HIV Tridot test.
13. To perform radial immuno diffusion test.
14. To perform immuno precipitation method.
15. To perform HBs Ag rapid test.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 27


Om Sterling Global University
BHMT-423: Histopathology & Histotechniques-II -Practical
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

List of Experiments:

1. Grossing of tissue
2. To perform tissue processing by manual method.
3. To perform section cutting of paraffin embedded tissue.
4. To fix the smear on glass slide.
5. To perform hematoxylin and eosin staining.
6. To perform PAS staining.
7. To perform AFB staining

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 28


Om Sterling Global University
BHMT-424: Comprehensive Viva
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

Oral Examination of all theory subjects of fourth semester.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 29


Om Sterling Global University
B.Sc. Medical Lab Technology

Fifth Semester

BHMT-501: Immunohematology & Blood Banking


Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Basic Principles of Blood Banking, Antigen, Antibody, naturally occurring antibody,
Complement, ABO & Rh blood group system, Methods of blood group determination,
Forward and Reverse grouping, Slide & Tube method, Gel method.

Unit-II
Other blood group system such as Lewis, MNS, Kell Duffy etc. Anticoagulants and
preservative used in blood bank, Donor selection criteria, Blood collection and processing

Unit-III
Transfusion transmissible infectious disease screen, Coomb’test, Cross matching,
Compatibility testing, Antibody Screening & Identification, Grading of Reaction/Agglutination

Unit-IV
Blood components and its preparation, preservation, storage and transportation Indications for
different blood component transfusion, Blood transfusion reaction and its type, HDN
Introduction of stem cell banking and bone marrow transplantation.
Text & Reference Books:
1. Godkar.B. Praful,(2016) Textbook of MLT,3rd edition,Bhalani Publications
2. Ochei J & Kolhatkar A(2000),Medical Laboratory Science: Theory & Practice, 3rd
edition,Mcgraw Hill Education
3. Mukherjee .L.K(2017), Medical Laboratory Technology,Vol.1-3, 3rd edition, Tata Mcgraw
Hill

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 30


Om Sterling Global University
BHMT-502: Clinical Enzymology & Automation
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Coenzyme Classification, various types and function, structure of NAD+, NADP+, FAD and FMN,
PPP. Units for measuring enzyme activity, factors affecting enzyme level in serum/ plasma.
Clinical assay & its type, kinetic assay and end point assay for the enzymes

Unit-II
Enzyme kinetics, the Michaelis-Menten equation and its physiological significances, Enzyme
Inhibition, types of inhibitors of enzyme

Unit-III
Isoenzymes, their tissue distribution and clinical significance: ALT, AST, ALP, GGT, CPK, CK-
MB, LDH,Troponin, Myoglobin, Amylase, Lipase, ACP,

Unit-IV
Basic Concepts of Automation, principle, working and maintenance of various clinical chemistry
analyzers, point of care testing, Hospital Laboratory Management Learning Outcome: Students
would be able to understand contemporary methods and -Practical approaches that are used in the
clinical laboratories for the investigation of the diseased state as well as application of automation
in laboratory.

Text & Reference Books:


1. D M Vasudevan, (2011),Text book of Medical Biochemistry,6th edition Jaypee Publishers
2. M N Chatterjea & Rana Shinde,(2012),Text book of Medical Biochemistry,8th edition,Jayppe
Publications
3. Singh & Sahni,(2008),Introductory -Practical Biochemistry,2nd edition, Alpha science
4. Lehninger,(2013),Principles of Biochemistry,6th edition, W H Freeman

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 31


Om Sterling Global University
BHMT-503: Parasitology & Virology
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I

Diagnostic methods in Parasitology: Introduction, Examination of stool, urine, blood, Culture


methods, Immunological diagnosis and serology

Unit-II
Nature and Properties of Viruses Introduction: Discovery of viruses, nature and definition of
viruses, general properties, concept of viroids, virusoids, satellite viruses and Prions. Structure of
Viruses: Capsid symmetry, enveloped and non-enveloped viruses Isolation, purification and
cultivation of viruses Viral taxonomy: Classification and nomenclature of different groups of
viruses, Modes of viral transmission: Persistent, non-persistent, vertical and horizontal Viral
multiplication and replication strategies: Interaction of viruses with cellular receptors and entry of
viruses. Assembly, maturation and release of virions.

Unit-III
Poxviruses, Herpes viruses, hepaptitis viruses, retroviruses-HIV, Picorna viruses, rhabdoviruses,
orthomyxoviruses and paramyxo viruses, TORCH profile, Symptoms, mode of transmission,
prophylaxis and control of Polio, Herpes, Hepatitis, Rabies, Dengue, HIV, Influenza with brief
description of swine flu, Ebola, Chikungunya, Japanese Encephalitis

Unit-IV
Introduction to oncogenic viruses, Types of oncogenic DNA and RNA viruses, concepts of
oncogenes and proto-oncogenes, prevention & control of viral diseases, antiviral compounds and
their mode of action, interferon and their mode of action, General principles of viral vaccination
Text & Reference Books:

1. Ananthanarayan R. and Paniker C.K.J. (2009) Textbook of Microbiology. 8th edition,


University Press Publication
2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013)
3. Adelberg’s Medical Microbiology. 26th edition. McGraw Hill Publication

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 32


Om Sterling Global University
BHMT-504: Diagnostic Cytology
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Cell basic structure and function, cell organelles, cell cycle, Benign and Malignant tumors,
Instruments used in cytology, preparation of buffers, stains microscopy: Light, compound, phase
contrast, fluorescence .

Unit-II
Instruments and equipments used in cytology Fixation and Fixatives used in cytology, Adhesive
and mounting media, Cell block and cytospin technique, Staining such as PAP, Diff-quick, MGG,
H&E, Shorr staining, significance of PAPHPV, Destaining and restaining of slides, Cover
slipping .

Unit-III
Aspiration and exfoliative cytology, Patient preparation, Sample collection, Fixation, Processing
and Staining FNAC, collection, processing of sample and staining, on site quick staining
procedure .

Unit-IV
Pap staining, Progressive & Regressive, Hormonal cytology in different age groups, Collection
and processing of sputum, BAL, CSF, Pleural, peritoneal and pericardial fluid, Gynaecologic
sample.

Text & Reference Books:


1.Bibbo, (1997),Comprehensive Cytopathology,2nd edition, Saunders Publishers
2. Koss’s Diagnostic Cytology,Vol.1 & 2,(2006),5th edition, Lippincott

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 33


Om Sterling Global University
BHMT-505: Principles of Laboratory Management & Medical
Ethics
Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Ethical Principles and standards for a clinical laboratory professional duty to the patient, duty to
colleagues and other professionals, Good Laboratory Practice (GLP) ,Introduction to Basics of
GLP and Accreditation, Aims of GLP and Accreditation, Advantages of Accreditation, Brief
knowledge about National and International Agencies for clinical laboratory accreditation

Unit-II
Awareness/Safety in a clinical laboratory, General safety precautions. HIV: pre- and post-
exposure guidelines, Hepatitis B & C: pre- and post-exposure guidelines, Drug Resistant
Tuberculosis Patient management for clinical samples collection, transportation and preservation,
Sample accountability, Purpose of accountability, Methods of accountability

Unit-III
Sample analysis: Introduction, factors affecting sample analysis, reporting results, basic format of
a test report, reported reference range, clinical alerts, abnormal results, results from referral
laboratories, release of examination results, alteration in reports

Unit-IV
Quality Management system: Introduction, Quality assurance, Quality control system, Internal
and External quality control, quality control chart Biomedical Introduction and importance of
calibration and Validation of Clinical Laboratory instrument Ethics in Medical laboratory
Practice, Ethics in relation to Pre-Examination procedures, Examination procedures, reporting of
results, preserving medical records Procurement of equipment and Inventory Control

Text & Reference Books:


1.Teitz,(2007),Fundamentals of Clinical Chemistry,6th edition, Elsevier Publications
2. Bishop(2013),Clinical Chemistry,7th edition, Wiley Publications
3. Henry's Clinical Diagnosis and Management by Laboratory Methods,(2011),22nd edition,
Elsevier

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 34


Om Sterling Global University
BHMT-521: Clinical Enzymology & Automation -Practical

Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1. To perform enzyme estimation of LFT


2. To perform enzyme estimation of Cardiac profile
3. Determination of Troponin I
4. To perform enzyme estimation of Pancreatic disorder
5. To perform estimation of ACP.
6. Antenatal profile
7. Estimation of bicarbonate
8. Arterial blood gas analysis
9. Determination of Calcium
10. Creatinine and urea clearance test

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 35


Om Sterling Global University
BHMT-522: Parasitology & Virology -Practical
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1.Leishman staining for malarial parasites


2. Demonstration of permanent slide of Trichuris, Ascaris and Hookworm
3. Saline wet mount for observing ova and eggs of parasites.
4. Iodine wet mount for observing ova and eggs of parasites.
5. Concentration of stool samples by floatation method
6. Zinc sulphate conc. Method for stool sample
7. Demonstration of various parasites by permanent slides.
8. Concentration of stool sample by sedimentation method
9. Serological diagnosis of Leishmania
10. Aldehyde Chopra test for Kala Azar

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 36


Om Sterling Global University
BHMT-523: Diagnostic Cytology -Practical
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

List of Experiments:

1. Preparation of various cytological fixatives


2. Preparation of various stains used in cytology
3. Preparation of smear
4. To perform PAP staining
5. To perform Giemsa staining on fluid sample
6. To prepare cell suspension
7. Processing of various fluid samples

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 37


Om Sterling Global University
Sixth Semester

BHMT-601: Clinical Endocrinology &Toxicology


Credit: 04 Max. Marks: 100 (Internal: 40, External: 60)

4L+0T+0P End Term Exam.: 3 Hours

Unit-I
Hormones, Classification of hormones, organs of endocrine system their secretion and
function, regulation of hormone secretion, Mechanism of action

Unit-II
Thyroid function test: Thyroid hormones, biological function, hypothyroidism,
hyperthyroidism, Determination of T3, T4, TSH, FT3, FT4, TBG, Disorder associated with
thyroid dysfunction.

Unit-III
Infertility profile: LH, FSH, TSH, Estrogen, Progesterone, Total Testosterone, Free
testosterone, DHEA-S, 17- Ketosteroids, Prolactin, their estimation and clinical significance,
reference range, hypo and hyper secretion, Triple Test

Unit-IV
Growth hormone, ACTH, Aldosterone, Cortisol their estimation and clinical significance,
reference range, hypo and hyper secretion

Text & Reference Books:


1. Teitz,(2007),Fundamentals of Clinical Chemistry,6th edition,Elsevier Publications
2. Bishop(2013),Clinical Chemistry,7th edition, Wiley Publications
3. Henry's Clinical Diagnosis and Management by Laboratory Methods,(2011),22nd
edition,Elsevier
4. D M Vasudevan, (2011),Text book of Medical Biochemistry,6th edition Jaypee Publishers

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 38


Om Sterling Global University
BHMT-602: Advanced Diagnostic Techniques
Credit: 03 Max. Marks: 100 (Internal: 40, External: 60)

3L+0T+0P End Term Exam.: 3 Hours

Unit-I
Chromatography, its principle, types and applications. Course Chromatography, Thin layer
chromatography, HPLC, Gas liquid chromatography, Ion exchange chromatography and their
application in diagnosis.

Unit-II
Basic Principle of electrophoresis, Course electrophoresis, Gel electrophoresis, PAGE, SDS-
PAGE, Agarose gel electrophoresis, buffer systems in electrophoresis. Electrophoresis of proteins
and nucleic acids, haemoglobin, immunoglobulin’s, isoenzymes Applications of electrophoresis
in clinical diagnosis.

Unit-III
Centrifugation, fixed angle and swinging bucket rotors , RCF and sedimentation coefficient,
differential centrifugation, density gradient centrifugation and Ultra centrifugation.

Unit-IV
Radioisotopes, Radioactivity, instruments for radioactivity measurement, applications of
radioisotopes in clinical biochemistry

Text & Reference Books:


1. Teitz,(2007),Fundamentals of Clinical Chemistry,6th edition, Elsevier Publications
2. Henry's Clinical Diagnosis and Management by Laboratory Methods,(2011),22nd
edition,Elsevier

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 39


Om Sterling Global University
BHMT-603: Diagnostic Molecular Biology
Credit: 03 Max. Marks: 100 (Internal: 40, External: 60)

3L+0T+0P End Term Exam.: 3 Hours

Unit-I

Basic transcription apparatus, Initiation, elongation and termination of transcription, Eukaryotic


Transcription of mRNA, tRNA and rRNA, types of RNA polymerases, transcription factors
Introduction of translation Unit-III Nucleic acid amplification testing, PCR, Principle, Types,
applications, Thermal cycle, RTPCR, reverse transcriptase PCR, Nested PCR

Unit-II
Blotting techniques, southern blotting and Western blotting Introduction to chromosomes, its
structure and disorder, Karyotyping, Chromosomal studies in hematological disorders (PBLC and
Bone marrow), FISH

Unit-III
Radioisotopes and its application in measurement of blood volume, determination of red cell
volume and plasma volume, red cell life span, platelet life span, radiation hazards and its
prevention disposal of radioactive material Introduction and applications of Flow cytometry, Stem
cell banking, Prenatal Diagnosis.

Unit-IV
Nucleic acid amplification testing, PCR, Principle, Types, applications, Thermal cycle, RTPCR,
reverse transcriptase PCR, Nested PCR

Text & Reference Books:


1. Teitz,(2007),Fundamentals of Clinical Chemistry,6th edition, Elsevier Publications
2. Henry's Clinical Diagnosis and Management by Laboratory Methods,(2011),22nd edition,
Elsevier
3. Singh & Sahni, (2008),Introductory -Practical Biochemistry,2nd edition, Alpha science
4. Lehninger, (2013),Principles of Biochemistry,6th edition, W H Freeman

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 40


Om Sterling Global University
BHMT-604: Medical Pathology
Credit: 03 Max. Marks: 100 (Internal: 40, External: 60)

3L+0T+0P End Term Exam.: 3 Hours

Unit-I
An Overview of Quality Control: Introduction, Random selection audit, Pathology QC, New
methods, Quality of performance of diagnostic pathologists

Unit-II
Frozen Section and Tissue Processing Techniques: Frozen and related sections, Frozen
sections and their uses, Freeze drying and freeze substitution, Frozen section substitution,
Preparation of frozen sections,Staining of fat

Unit-III
Application of Microwave Technology to Pathology: Introduction, Microwave staining,
Microwave processing, Microwave fixation, Microwave antigen retrieval

Unit-IV
Museum Techniques: Preparation of specimen, Storage of specimens, Mounting of museum
specimen

Text & Reference Books:


1.Textbook of Pathology by Shinde,9th edition, Wiley Publication

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 41


Om Sterling Global University
BHRM-651: Research Methodology & Biostatistics
Credit: 03 Max. Marks: 100 (Internal: 40, External: 60)

3L+0T+0P End Term Exam.: 3 Hours

Unit-I
Introduction to research and literature review : Definition of research, Purposes of research,
Need of research, Contents of research, What are concepts of research (Objectives and
Motivation), Types of research, research approaches, Research and Scientific method, Research
Flow, Process of research, Criteria of good research, problems encountered by researchers in
India, defining a research problem, components of a research problem, selecting the problem,
techniques involved in defining a problem, Problem statement, Research terminology,
Formulation, Setting Objectives, hypothesis

Unit-II
Research design: principles of research design, need and features of a good research design,
different research designs, Research strategies, Basic and applied research, Quantitative research
Sampling: Sampling frame, sampling design
Methods of data collection Classification of data collection methods, methods of data collection,
Validity and reliability of measuring instruments

Unit-III
Qualitative research different methods overview
Data Analysis Condensing data, analysis of data through descriptive and inferential statistics
Construction of research proposal, Mechanics of reports writing.

Unit-IV
Basics of Biostatistics: Introduction of Biostatistics, Sampling, Statistical significance,
Correlation Sample size determination. Statistics - presentation including classification and
diagrammatic representation –frequency distribution. Measures of central tendency; measures of
dispersion .Non Parametric Test

Text & Reference Books:


1.Mausner & Bahn: Epidemiology-An Introductory text, 2nd Ed., W. B. Saunders Co.

2. Richard F. Morton & J. Richard Hebd: A study guide to Epidemiology and Biostatistics, 2nd

Ed., University Park Press, Baltimore.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 42


Om Sterling Global University
BHMT-621: Clinical Endocrinology & Toxicology -Practical

Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1. To determine T3 conc. in serum sample.


2. To determine T4 conc. in serum sample.
3. To determine TSH conc. in serum sample.
4. To determine LH conc. in serum sample.
5. To determine FSH conc. in serum sample.
6. To determine Prolactin conc. in serum sample.
7. To determine TSH conc. in serum sample.
8. To perform TRIPLE test.
9. Demonstration of male and female infertility test.
10. Beta HCG

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 43


Om Sterling Global University
BHMT-622: Advanced Diagnostic Techniques -Practical
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

1.To perform separation of amino acids by Course chromatography


2. To perform separation of amino acids by thin layer chromatography
3. To perform separation of DNA by Agarose gel electrophoresis.
4. Separation of protein by PAGE
5. Separation of protein by Course electrophoresis
6. Separation of hemoglobin

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 44


Om Sterling Global University
BHMT-623: Diagnostic Molecular Biology -Practical
Credit: 01 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+2P End Term Exam.: 3 Hours

List of Experiments:

1. Isolation of DNA
2. Separation of DNA by Agarose gel electrophoresis
3. Demonstration of thermal cycler and PCR.
4. HIV test by Western Blotting
5. To perform karyotyping
6. Demonstration of PCR HLA B-27
7. Demonstration of PCR HIV
8. Demonstration of PCR MTB

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 45


Om Sterling Global University
BHMT-624: Clinical Posting (Orientation)

Credit: 02 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+4P End Term Exam.: 3 Hours

Student will deputed to various labs of Pathology department where they will observe following:

1.Clinical sample collection e.g. Blood, Urine, Stool, Saliva, Sputum .


2. Sample accountability- Labeling of sample, Making entries in Laboratory records.
3. Reporting results- Basic format of a test report, Release of examination results, Alteration in
reports. ‘
4. Quality Management system- Quality assurance, Internal and External quality control, Quality
improvement.
5. Biomedical waste management in a clinical laboratory - Disposal of used samples, reagents
and other biomedical waste.
6.Calibration and Validation of Clinical Laboratory instruments.
7.Pre-Examination procedures, Examination procedures, Reporting of results, Preserving
medical records, Access to medical laboratory records

Evaluation
Attendance and quality of participation ,Submission of Case reports(Log Book) in different areas

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 46


Om Sterling Global University
BHMT-625: Project Work -I

Credit: 02 Max. Marks: 100 (Internal: 50, External: 50)

0L+0T+4P End Term Exam.: 3 Hours

Objective:

The purpose of the project is to provide the student with training in academic research and
acquisition of (-Practical) skills, including the design of a research project, planning of
experiments, dealing with (-Practical) problems, recording of, presenting and analyzing data.

Scope:

During this semester student has to prepare synopsis and make presentation in front of experts.

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 47


Om Sterling Global University
B.Sc. Medical Lab Technology

Seventh Semester

BHMT-721: Internship
Credit: 12 Max. Marks: 600 (Internal: 300, External: 300)

0L+0T+24P End Term Exam.: 3 Hours

The internship time period provides the students the opportunity to continue to develop
confidence and increased skill in diagnosis and management. Students will demonstrate
competence in beginning, intermediate, and advanced procedures in above areas. Students will
participate in advanced and specialized treatment procedures. The student will complete the
clinical training by practicing all the skills learned in classroom and clinical instruction. The
students are expected to work for minimum 6 hours per day and this may be more depending on
the need and the healthcare setting.

Students shall be deputed to various labs of Pathology department wherein they shall undergo -
Practical training of handling patients, collection and processing of blood, urine, sputum stool
and body fluids samples. Identification of patient’s particulars based on CR number, Lab
Number and transfer of samples from collection centres to different labs. Process of performing
various tests in different labs.

Evaluation
Attendance and quality of participation ,Submission of Case reports(Log Book) in different areas

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 48


Om Sterling Global University
BHMT-722: Project Work -II

Credit: 06 Max. Marks: 300 (Internal: 150, External: 150)

0L+0T+12P End Term Exam.: 3 Hours

The research project is to be carried out over a period of approximately 6 months and will be
carried out in the hospitals, subject to approval by all concerned. Each student will select
research project with their respective supervisors. The projects will be selected such that a
student can reasonably be expected to make an original contribution to the chosen area of
research within the time period allotted &submit the final report and make presentation in front
of the experts.

Assessment is done on basis of work done ,presentation and viva.

Format of Dissertation

1. Abstract

2. Introduction

3. Aim & Objectives

4. Review of Literature

5. Materials & Methodology

6. Results

7. Discussion

8. Conclusion

9. Bibliography

10. Appendices

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 49


Om Sterling Global University
For International Students
B.Sc. Medical Lab Technology

8th Semester

BHMT-821: Special Internship with Research Project

Credit: 12 Max. Marks: 600 (Internal: 300, External: 300)

0L+0T+24P End Term Exam.: 3 Hours

The internship time period provides the students the opportunity to continue to develop
confidence and increased skill in diagnosis and management. Students will demonstrate
competence in beginning, intermediate, and advanced procedures in above areas. Students will
participate in advanced and specialized treatment procedures. The student will complete the
clinical training by practicing all the skills learned in classroom and clinical instruction. The
students are expected to work for minimum 6 hours per day and this may be more depending on
the need and the healthcare setting.

Students shall be deputed to various labs of Pathology department wherein they shall undergo -
Practical training of handling patients, collection and processing of blood, urine, sputum stool
and body fluids samples. Identification of patient’s particulars based on CR number, Lab
Number and transfer of samples from collection centres to different labs. Process of performing
various tests in different labs.

The research project is to be carried out over a period of approximately 1 year and will be carried
out in the hospitals, subject to approval by all concerned. Each student will select research
project with their respective supervisors. The projects will be selected such that a student can
reasonably be expected to make an original contribution to the chosen area of research within the
time period allotted. Assessment is done on basis of work done ,presentation and viva.

Evaluation
Attendance and quality of participation , Submission of Case reports in different areas

Scheme & Syllabus of B.Sc. MLT From Session 2020-21 onwardsPage 50

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