SAFETY DATA SHEET

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1. Identification of the substance/mixture and of the company/undertaking

Identification of the substance/preparation

Product code 15596018

Product name TRIZOL REAGENT

Company/Undertaking Identification

Life Technologies INVITROGEN CORPORATION

5791 VAN ALLEN WAY 5250 MAINWAY DRIVE
PO BOX 6482 BURLINGTON, ONT
CARLSBAD, CA 92008 CANADA L7L 6A4
+1 760 603 7200 800/263-6236

24 hour Emergency Response (Transport): 866-536-0631

301-431-8585
Outside of the U.S. +1-301-431-8585

For research use only. Not intended for human or animal diagnostic or therapeutic uses.

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Product code 15596018 Product name TRIZOL REAGENT

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 2. Hazards identification

GHS - Classification

Signal Word
DANGER

Health Hazard
Acute oral toxicity Category 3
Acute dermal toxicity Category 3
Acute Inhalation Toxicity - Dusts and Mists Category 2
Skin Corrosion/Irritation Category 1 B
Serious Eye Damage/Eye Irritation Category 1
Specific target organ systemic toxicity (repeated exposure) Category 2

Mutagenicity Mutagenic Category 2

Physical Hazards
not hazardous

Hazard statements
H330 - Fatal if inhaled
H301 - Toxic if swallowed
H311 - Toxic in contact with skin
H314 - Causes severe skin burns and eye damage
H341 - Suspected of causing genetic defects
H373 - May cause damage to organs through prolonged or repeated exposure
H412 - Harmful to aquatic life with long lasting effects

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Product code 15596018 Product name TRIZOL REAGENT

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Precautionary statements
P301 + P330 + P331 - IF SWALLOWED: rinse mouth. Do NOT induce vomiting
P301 + P310 - IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician
P304 + P340 - IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if
present and easy to do. Continue rinsing
P303 + P361 + P353 - IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with
water/shower
P309 + P311 - IF exposed or if you feel unwell: Call a POISON CENTER or doctor/physician
P403 + P233 - Store in a well-ventilated place. Keep container tightly closed
P280 - Wear protective gloves/protective clothing/eye protection/face protection
P284 - Wear respiratory protection
P271 - Use only outdoors or in a well-ventilated area
P260 - Do not breathe dust/fume/gas/mist/vapors/spray
P264 - Wash hands thoroughly after handling
P273 - Avoid release to the environment
EUH032 - Contact with acids liberates very toxic gas

Principle Routes of Exposure/

Potential Health effects
Eyes Causes burns. Risk of serious damage to eyes. Corrosive to the eyes and may
cause severe damage including blindness.
Skin Toxic in contact with skin. Causes burns. Components of the product may be
absorbed into the body through the skin. Irritating to skin and mucous membranes.
Possible risks of irreversible effects.
Inhalation Toxic by inhalation. Causes burns.
Ingestion Toxic if swallowed. Ingestion causes burns of the upper digestive and respiratory
tracts. Ingestion may cause gastrointestinal irritation, nausea, vomiting and
diarrhea.

Specific effects
Carcinogenic effects Phenol has been classified by the International Agency for Research on Cancer
(IARC) as not classifiable as to carcinogenicity to humans (Group 3).
Mutagenic effects not applicable
Reproductive toxicity not applicable
Sensitization not applicable

Target Organ Effects Skin

Lungs
Liver
spleen
Kidney

HMIS
Health 3 * Chronic Hazard
Flammability 1
Reactivity 0

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 3. Composition/information on ingredients
Chemical Name CAS-No EINECS-No Weight %
Phenol 108-95-2 203-632-7 30-60
Guanidine isothiocyanate 593-84-0 209-812-1 15-40
Ammonium thiocyanate 1762-95-4 217-175-6 7-13
Contact with acids or bleach liberates toxic gases. DO NOT ADD acids or bleach to any liquid wastes containing this
product. We recommend handling all chemicals with caution.

4. First aid measures

Skin contact Wash off immediately with soap and plenty of water removing all contaminated
clothes and shoes. Call a physician immediately.
Eye contact IF IN EYES: Rinse cautiously with water for several minutes. Remove contact
lenses, if present and easy to do. Continue rinsing. Rinse immediately with plenty
of water, also under the eyelids, for at least 15 minutes. Call a physician
immediately.
Ingestion Call a physician or Poison Control Centre immediately. Rinse mouth. Do not
induce vomiting without medical advice. Never give anything by mouth to an
unconscious person.
Inhalation Toxic by inhalation. Move to fresh air. Call a physician or Poison Control Centre
immediately.
Notes to physician Treat symptomatically.

5. Fire-fighting measures

Suitable extinguishing media Dry chemical. Carbon dioxide (CO2). Water spray. Foam.
Special protective equipment for firefighters Wear self-contained breathing apparatus and protective
suit.

Australia HazChem Code 2X

6. Accidental release measures

Personal precautions ELIMINATE all ignition sources (no smoking, flares, sparks or flames in immediate
area). Use personal protective equipment. Avoid contact with skin, eyes and
clothing. Ensure adequate ventilation. Keep people away from and upwind of
spill/leak. Evacuate personnel to safe areas.
Methods for cleaning up Prevent product from entering drains. Soak up with inert absorbent material.
Neutralise with slaked lime, sodium bicarbonate or crushed limestone. Pick up and
transfer to properly labelled containers.

Environmental precautions

Prevent further leakage or spillage if safe to do so. Prevent product from entering drains. Do not allow material to
contaminate ground water system.

See Section 12 for additional information.

7. Handling and storage

Handling Always wear reccommended Personal Protective Equipment. Prevent contact with
skin, eyes and clothing. Remove all sources of ignition.
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Product code 15596018 Product name TRIZOL REAGENT

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Storage Keep containers tightly closed in a cool, well-ventilated place. Keep away from
open flames, hot surfaces and sources of ignition. Protect from light.

8. Exposure controls/personal protection

Exposure limits

Chemical Name OSHA PEL OSHA PEL (Ceiling) ACGIH OEL (TWA) ACGIH OEL (STEL)
Phenol 19 mg/m3 - 5 ppm -
5 ppm

Engineering measures Use in a chemical fume hood.

Personal protective equipment

Respiratory protection In case of insufficient ventilation wear suitable respiratory equipment.

Respirator Up to 50 ppm:
Recommendations, National (APF = 10) Any air-purifying half-mask respirator with organic vapor cartridge(s) in
Institue of Occupational combination with an N95, R95, or P95 filter. The following filters may also be used:
Safety and Health, U.S. N99, R99, P99, N100, R100, P100.
(APF = 10) Any supplied-air respirator
Up to 125 ppm:
(APF = 25) Any supplied-air respirator operated in a continuous-flow mode
(APF = 25) Any powered air-purifying respirator with an organic vapor cartridge in
combination with a high-efficiency particulate filter.
Up to 250 ppm:
(APF = 50) Any air-purifying full-facepiece respirator equipped with organic vapor
cartridge(s) in combination with an N100, R100, or P100 filter.
(APF = 50) Any air-purifying, full-facepiece respirator (gas mask) with a chin-style,
front- or back-mounted organic vapor canister having an N100, R100, or P100
filter.
(APF = 50) Any powered, air-purifying respirator with a tight-fitting facepiece and
organic vapor cartridge(s) in combination with a high-efficiency particulate filter
(APF = 50) Any self-contained breathing apparatus with a full facepiece
(APF = 50) Any supplied-air respirator with a full facepiece
Emergency or planned entry into unknown concentrations or IDLH conditions:
(APF = 10,000) Any self-contained breathing apparatus that has a full facepiece
and is operated in a pressure-demand or other positive-pressure mode
(APF = 10,000) Any supplied-air respirator that has a full facepiece and is
operated in a pressure-demand or other positive-pressure mode in combination
with an auxiliary self-contained positive-pressure breathing apparatus
Escape:
(APF = 50) Any air-purifying, full-facepiece respirator (gas mask) with a chin-style,
front- or back-mounted organic vapor canister having an N100, R100, or P100
filter. /Any appropriate escape-type, self-contained breathing apparatus

Hand protection Impervious gloves. S24 - Avoid contact with skin. S36 - Wear suitable protective
clothing.
Eye protection Tightly fitting safety goggles.
Skin and body protection. impervious clothing.
Hygiene measures Contaminated work clothing should not be allowed out of the workplace. Avoid
contact with skin, eyes and clothing. Handle in accordance with good industrial
hygiene and safety practice.

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Environmental exposure Prevent product from entering drains.
controls

9. Physical and chemical properties

General Information
Form liquid
Appearance red, Maroon
Odor Medicinal, sweet, Tar like
Boiling Point/Range °C no data available °F no data available
Melting point/range °C no data available °F no data available
Flash point °C no data available °F no data available
Autoignition temperature °C no data available °F no data available
Oxidizing properties No information available.
Water solubility soluble

10. Stability and reactivity

Stability Stable under normal conditions.

Materials to avoid Strong oxidizing agents Strong acids Isocyanates. Heat Nitriles, Nitrides. Alkaline
earth metals Strong oxidizers, alkali metals and alkaline earth metals may cause
fires or explosions.
Hazardous decomposition Toxic gases Sulphur oxides Hydrogen cyanide (hydrocyanic acid) Carbon oxides,
products Nitrogen Oxides
polymerization Hazardous polymerisation does not occur.

11. Toxicological information

Acute toxicity

Chemical Name LD50 (oral,rat/mouse) LD50 (dermal,rat/rabbit) LC50 (inhalation,rat/mouse)

Phenol = 317 mg/kg (Rat) no data available =316mg/m3(Rat)
Guanidine isothiocyanate 571 mg/kg 2000 mg/kg 5.319 mg/L (4H)
Ammonium thiocyanate = 500 mg/kg (Rat) no data available no data available

Principle Routes of Exposure/

Potential Health effects
Eyes Causes burns Risk of serious damage to eyes Corrosive to the eyes and may
cause severe damage including blindness.
Skin Toxic in contact with skin Causes burns Components of the product may be
absorbed into the body through the skin Irritating to skin and mucous membranes
Possible risks of irreversible effects.
Inhalation Toxic by inhalation Causes burns.
Ingestion Toxic if swallowed Ingestion causes burns of the upper digestive and respiratory
tracts Ingestion may cause gastrointestinal irritation, nausea, vomiting and
diarrhea.

Carcinogenic effects Phenol has been classified by the International Agency for Research on Cancer
(IARC) as not classifiable as to carcinogenicity to humans (Group 3).
Mutagenic effects No information available.
Reproductive toxicity No information available.
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 Sensitization No information available.

Target Organ Effects Skin Lungs Liver spleen Kidney

12. Ecological information

Ecotoxicity effects Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic
environment.
Mobility see log Pow
Biodegradation Inherently biodegradable
Bioaccumulation No information available.

Chemical Name Freshwater Algae Water Flea Data Freshwater Fish Microtox Data log Pow
Data Species Data
Phenol Pseudokirchneriella Daphnia magna logPow1.47
subcapitata EC504.24 - 10.7 mg/L
108-95-2 EC50=46.42 mg/L (96 (48 h) Daphnia magna
h) Pseudokirchneriella EC5010.2 - 15.5 mg/L
subcapitata (48 h)
EC500.0188 - 0.1044
mg/L (96 h)
Desmodesmus
subspicatus EC50187
- 279 mg/L (72 h)

13. Disposal considerations

Dispose of in accordance with local regulations

S29 - Do not empty into drains
S57 - Use appropriate container to avoid environmental contamination

14. Transport information

IATA
Proper shipping name Corrosive liquid, n.o.s. (guanidine thiocyanate-phenol solution)
Hazard class 8
Subsidiary Class none
Packing group II
UN-No UN1760
ERG Code 153

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Product code 15596018 Product name TRIZOL REAGENT

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 15. Regulatory information

Component TSCA
Phenol Listed
108-95-2 ( 30-60 )
Guanidine isothiocyanate Listed
593-84-0 ( 15-40 )
Ammonium thiocyanate Listed
1762-95-4 ( 7-13 )

U.S. Federal Regulations

SARA 313
This product contains the following toxic chemical(s) subject to the notification requirements of section 313 of Title III of the
Superfund Amendments and Reauthorization Act of 1986. This law requires certain manufacturers to report on annual emissions of
specified chemicals and chemical categories. Please note that if you repackage, or otherwise redistribute, this product to industrial
customers, a notice similar to this one should be sent to those customers:
Chemical Name CAS-No Weight % SARA 313 - Threshold Values
Phenol 108-95-2 30-60 1.0

Clean Air Act, Section 112 Hazardous Air Pollutants (HAPs) (see 40 CFR 61)
This product contains the following HAPs:

Component CAS-No Weight % HAPS data

Phenol 108-95-2 30-60 Present
108-95-2 ( 30-60 )

U.S. State Regulations

Chemical Name Massachusetts - RTK New Jersey - RTK Pennsylvania - RTK Illinois - RTK Rhode Island - RTK
Phenol Listed Listed Listed Listed Listed
Ammonium thiocyanate Listed - Listed - -

California Proposition 65
This product does not contain chemicals listed under Proposition 65

WHMIS hazard class:

D1A Very toxic materials
E Corrosive material

This product has been classified according to the hazard criteria of the CPR and the MSDS contains all of the information required by the CPR

16. Other information

Reason for Revision not applicable. (M)SDS sections updated.

For research use only.

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The above information was acquired by diligent search and/or investigation and the recommendations are based on
prudent application of professional judgment. The information shall not be taken as being all inclusive and is to be
used only as a guide. All materials and mixtures may present unkown hazards and should be used with caution.
Since the Company cannot control the actual methods, volumes, or conditions of use, the Company shall not be held
liable for any damages or losses resulting from the handling or from contact with the product as described herein.
THE INFORMATION IN THIS MSDS DOES NOT CONSTITUTE A WARRENTY, EXPRESSED OR IMPLIED,
INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PUPOSE.

End of Safety Data Sheet

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Product code 15596018 Product name TRIZOL REAGENT

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