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Class 11. Bioremediation

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ENVIRONMENTAL STUDIES & LIFE SCIENCES

Jyoti Kumari
Department of Biotechnology
ENVIRONMENTAL STUDIES & LIFE SCIENCES

Bioremediation

Jyoti Kumari
Department of Biotechnology
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

• The use of either naturally occurring or deliberately introduced


microorganisms to consume and break down environmental
pollutants, in order to clean a polluted site
• Employs the microorganisms, to degrade the pollutants and
convert them into less toxic or non-toxic form
• The suitable organisms can be bacteria, fungi, or plants, which
have the physiological abilities to degrade, detoxify, or render the
contaminants harmless.
• Biological method of remediation is an extremely attractive,
important, and productive alternative for cleaning, debugging,
managing, and rehabilitating and consequently ameliorating
contaminated environments via judicious utilization of microbial
activities
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

• Bioremediation technologies can be classified into two general


categories: ex situ and in situ
• The ex situ techniques require the physical removal of the
contaminated material and its transportation to another area
for further treatment by bioreactors, land farming, or
composting, whereas in situ technologies involve treatment of
contaminated material in place
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

https://www.frontiersin.org/files/Articles/937186/fsoil-02-937186-HTML/image_m/fsoil-02-937186-g003.jpg
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

https://www.frontiersin.org/files/Articles/937186/fsoil-02-937186-HTML/image_m/fsoil-02-937186-g003.jpg
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

Bioaugmentation Addition of bacterial cultures to a contaminated


medium; frequently used in bioreactors and ex
situ systems

Biostimulation Stimulation of indigenous microbial populations in


soils or groundwater by adding nutrients to the
existing bacteria; which can be performed either in
situ or ex situ
Bioreactors Biodegradation in a container or reactor; may be
used to treat several liquid wastes or slurries but
relatively high capital and operational cost
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

Bioventing Method of treating contaminated soils by drawing


oxygen through the soil to stimulate microbial growth
and activity

Composting Aerobic, thermophilic treatment process; can be


performed by using static piles, aerated piles, or
continuously fed reactors; extended treatment time

Land farming Solid-phase treatment system for contaminated soils;


may be performed in situ or in a constructed soil
treatment cell; cost-efficient
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

• Most bioremediation systems operate under aerobic


conditions; however, anaerobic conditions are also
applicable, thus enabling the degradation of recalcitrant
molecules by using specific microorganisms
• Mainly microorganisms, microbial or plants or its enzymes
are used to detoxify contaminants in the soil and other
environments
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

• Bioremediation, as a technique, can offer several advantages over


other more conventional treatment methods
• Firstly, bioremediation, as a natural process for the treatment of
wastes, is usually acceptable
• Suitable microbial populations can degrade a wide range of
contaminants, rendering a hazardous compound to a harmless one
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

• Eventually, the residues of the treatment may include


simpler compounds, such as carbon dioxide or water, but
also cell biomass
• The potential threats to human health and to the
environment are minimal
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

• Bioremediation, like any other technology, has certain


disadvantages
• In particular, it is limited only to those compounds that are
biodegradable
• The effectiveness of bioremediation is highly susceptible to the
microbial growth and other environmental parameters of the site
• Bioremediation often requires more time than other treatment
options
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

• Examples of bioremediation:
• Exxon Valdez spill, Prince William Sound, Alaska, 1989
• Deepwater Horizon oil spill, Gulf of Mexico, 2010
ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

Exxon Valdez spill

Image source: RGB Ventures


ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

Exxon Valdez spill


Prof. Chakrabarty genetically
engineered a new species
of Pseudomonas bacteria
("the oil-eating bacteria") in 1971
while working for the Research &
Development Center at General
Electric Company in Schenectady,
New York.

Image source: RGB Ventures


ENVIRONMENTAL STUDIES & LIFE SCIENCES
Bioremediation

Image source: RGB Ventures


ENVIRONMENTAL STUDIES & LIFE SCIENCES
PHYTOREMEDIATION

Phytoremediation is a bioremediation process that uses


various types of plants to remove, transfer, stabilize, and/or
destroy contaminants in the soil and groundwater. There are
several different types of phytoremediation mechanisms.
ENVIRONMENTAL STUDIES & LIFE SCIENCES
PHYTOREMEDIATION
ENVIRONMENTAL STUDIES & LIFE SCIENCES
PHYTOREMEDIATION
THANK YOU

Jyoti Kumari
Department of Biotechnology
pes1ps22bt005@pesu.pes.edu
+91 6203364966

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