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    NRBC

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    Dara Vinson
    This document discusses the precision and clinical importance of various hematological parameters and cell differentiation analyses. It provides reference ranges for white blood cells, red blood cells, hemoglobin, platelets, and reticulocytes in normal subjects and subjects with thalassemia or splenectomy. Precision data is given showing the mean, standard deviation, and coefficient of variation for parameters measured in normal subjects and subjects with thalassemia or splenectomy. The importance of nucleated red blood cell measurement in monitoring bone marrow activity and accurately counting white blood cells in splenectomized thalassemia patients is highlighted.

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    © All Rights Reserved
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    NRBC

    Uploaded by

    Dara Vinson
    121 views15 pages
    This document discusses the precision and clinical importance of various hematological parameters and cell differentiation analyses. It provides reference ranges for white blood cells, red blood cells, hemoglobin, platelets, and reticulocytes in normal subjects and subjects with thalassemia or splenectomy. Precision data is given showing the mean, standard deviation, and coefficient of variation for parameters measured in normal subjects and subjects with thalassemia or splenectomy. The importance of nucleated red blood cell measurement in monitoring bone marrow activity and accurately counting white blood cells in splenectomized thalassemia patients is highlighted.

    Original Description:

    CLINICAL PATHWAYS

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    This document discusses the precision and clinical importance of various hematological parameters and cell differentiation analyses. It provides reference ranges for white blood cells, red blood cells, hemoglobin, platelets, and reticulocytes in normal subjects and subjects with thalassemia or splenectomy. Precision data is given showing the mean, standard deviation, and coefficient of variation for parameters measured in normal subjects and subjects with thalassemia or splenectomy. The importance of nucleated red blood cell measurement in monitoring bone marrow activity and accurately counting white blood cells in splenectomized thalassemia patients is highlighted.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    121 views15 pages

    NRBC

    Uploaded by

    Dara Vinson
    This document discusses the precision and clinical importance of various hematological parameters and cell differentiation analyses. It provides reference ranges for white blood cells, red blood cells, hemoglobin, platelets, and reticulocytes in normal subjects and subjects with thalassemia or splenectomy. Precision data is given showing the mean, standard deviation, and coefficient of variation for parameters measured in normal subjects and subjects with thalassemia or splenectomy. The importance of nucleated red blood cell measurement in monitoring bone marrow activity and accurately counting white blood cells in splenectomized thalassemia patients is highlighted.

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    WBCs Differentiation

    nRBC Location
    Platelet Scattergram
    Linearity
    WBC 0.02 400 x 10
    3
    /L
    RBC 0.00 7.0 x 10
    6
    /L
    Hemoglobin 0.00 22.5 g / dL
    Platelet 5.0 3500 x10
    3
    /L
    Reticulocyte 0.2 24.5 %

    Within Run Precision
    Mean SD CV
    WBC (x10
    3
    /L) 7.5 0.2 2.7
    RBC (x10
    6
    /L) 5.0 .006 1.2
    Hb (g/dL) 15.0 0.14 0.93
    MCV (fL) 90 0.7 0.78
    Plt (x10
    3
    /L) 300 8.8 2.93
    Retic (%) 2.0 0.25 12.5
    Limitation of the nRBC count
    Sample : uncorrected WBC count 3.00x10
    3
    /L
    : 2% nRBCs orabsolute 0.20x10
    9
    / L

    Normal

    WBCB
    (x103
    cells/m
    L)

    Correct
    ecdWB
    CB
    (x10
    3
    cells/m
    L)
    RBC
    (x10
    6

    cells/mL)
    Hb
    (g/d
    L)
    HCT

    (%)
    MCV

    (fL)
    MCH

    (pg)
    MCHC

    (g/dL)
    RDW

    (%)
    Retic

    (x10
    9

    cells/L)
    Abs
    NRBC

    (x10
    9

    cell/mL
    )

    mean 7.43 4.53 12 36.08 86.0 26.38 33.09 15.66 15.55 0.00
    SD 0.17 0.08 0.07 0.38 1.05 0.39 0.24 0.08 3.00 0.00
    %
    CV 2.35 1.81 0.60 1.05 1.32 1.48 0.73 0.52 19.27 0.00



    -Thalssemia/Hb E disease with splenegtomy

    mean 70.60
    11.95
    3.36 7.75 27.11 80.61 23.03 28.59 26.40 579.63 58.65
    SD 0.83
    0.39
    0.05 0.07 0.33 0.54 0.26 0.26 0.14 48.51 0.89
    %
    CV 1.17
    3.29
    1.56 0.96 1.23 0.67 1.11 0.92 0.54 8.37 1.53
    Test Precision
    Normal
    -Thalassemia 1 trait
    -thalassemia/Hb E with splenectomy
    -thalassemia/Hb E with splenectomy
    Corrected WBC
    0
    20
    40
    60
    80
    100
    120
    W
    B
    C

    (
    x
    1
    0
    3

    c
    e
    l
    l
    /
    u
    l
    L
    Uncorrect WBC
    0
    20
    40
    60
    80
    100
    120
    W
    B
    C

    (
    x
    1
    0
    3

    c
    e
    l
    l
    /
    u
    l
    )

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a
    /
    H
    b

    E

    w
    i
    t
    h

    s
    p
    l
    e
    n
    e
    c
    t
    o
    m
    y

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a
    /
    H
    b

    E

    H
    b

    H

    H
    b

    H
    -
    C
    S

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a

    2

    t
    r
    a
    i
    t

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a

    1

    t
    r
    a
    i
    t

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a
    /
    H
    b

    E

    w
    i
    t
    h

    s
    p
    l
    e
    n
    e
    c
    t
    o
    m
    y

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a
    /
    H
    b

    E

    H
    b

    H

    H
    b

    H
    -
    C
    S

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a

    2

    t
    r
    a
    i
    t

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a

    1

    t
    r
    a
    i
    t

    r = 0.87
    The correlation of different technique
    The Importance of NRBC measurement

    - To monitor bone marrow activity
    (the same as reticulocyte)
    - To accurately count the number of
    white blood cell which is important
    in the monitor of drug toxicity in
    splenectomized thalassemia subjects
    (such as deferriprone, hydroxyurea)
    RBC
    0
    2
    4
    6
    8
    10
    R
    B
    C

    (
    x
    1
    0
    9

    c
    e
    l
    l
    /
    u
    L
    )

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a
    /
    H
    b

    E

    w
    i
    t
    h

    s
    p
    l
    e
    n
    e
    c
    t
    o
    m
    y

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a
    /
    H
    b

    E

    H
    b

    H

    H
    b

    H
    -
    C
    S

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a

    2

    t
    r
    a
    i
    t

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a

    1

    t
    r
    a
    i
    t

    Hb
    0
    2
    4
    6
    8
    10
    12
    14
    16
    H
    b

    (
    g
    /
    d
    L
    )

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a
    /
    H
    b

    E

    w
    i
    t
    h

    s
    p
    l
    e
    n
    e
    c
    t
    o
    m
    y

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a
    /
    H
    b

    E

    H
    b

    H

    H
    b

    H
    -
    C
    S

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a

    2

    t
    r
    a
    i
    t

    -
    T
    h
    a
    l
    a
    s
    s
    e
    m
    i
    a

    1

    t
    r
    a
    i
    t

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