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Endodontic Microbiology: Dr. Ammar Abu Mostafa

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ENDODONTIC

MICROBIOLOGY
ENDO 412 Lecture No 5 part II

Dr. Ammar Abu Mostafa


Learning outcomes
 Portals of entry to the pulp
 Bacterial role in Pulp and Peri-apical pathosis
 Enterococcus faecalis
 Absecess and Cellulitis
 Management of the infected teeth
Portals of Entry to the Pulp
 Caries
 Through anomalous tract like radicular lingual groove
 Through fracture line
 Via exposed dentin
 Around restorations
 From periodontal pockets to the pulp via apical
foramen, accessory and lateral canals
Pulp Necrosis & Bacterial Invasion
 Bacterial penetration into dentinal tubules of
necrotic teeth is greater than teeth with vital pulp,
due to the following reasons:
1. The presence of fluids in dentinal tubules
2. The presence of odontoblastic processes
3. Formation of tertiary dentin

 Vital pulp is more resistant to bacterial invasion than


necrotic pulp
Bacterial Role in Pulp Infection
 In an experimental study; after intentional pulp
exposure, pulp necrosis and periradicular lesion
developed in rats with normal microbial flora but not
in germ-free rats
 Microorganisms invade the necrotic pulp and infect
the root canal system, including the dentinal tubules

Coccal forms of bacteria seen in the


cross-section of a fractured dentinal tubule
 Most of the bacteria in an endodontic infection are
strict anaerobes
 With increasing time and depth of pulp infection, the
microbial flora changes from a predominantly
facultative, Gram-positive flora to an almost
completely anaerobic and mainly Gram-negative flora.
 Endodontic infections are polymicrobial, no single
microorganism or group of bacteria has been proven
to be more pathogenic than another.
 The following strains are commonly found in
endodontic infections: Fusobacterium , Prevotella,
Prophyromonas, Peptostreptococcus, and Veillonella.
 The microbial flora in retreatment cases is different
from bacteria in primary apical periodontitis. Typically
retreatment cases show enterococcus faecalis ,
streptococci, and anaerobic Gram-positive rods in high
frequencies.
 Fungi have been identified in root canals and in teeth
refractory to RCT.
 Viruses, including HIV, are also present in the pulp.
Bacterial Role in Periradicular Disease
 Bacteria and bacterial by-products of the root canal
play the major role of periradicular lesion
 The bacteria are polymicrobial and are similar to
those isolated from infected root canals
Resistance of Oral Microbes to
Medicaments
 Enterococci has been shown to be relatively
resistant to Ca(OH)2 and occur in high frequency in
retreatment cases
Abscess & Cellulitis
 Abscess is a cavity containing pus (purulent
exudate) consisting of bacteria, bacterial by-products,
inflammatory cells, numerous lysed cells, and the
contents of those cells.
 Cellulitis is a diffuse, erythematous, mucosal, or
cutaneous infection that may rapidly spread into
deep facial spaces and become life threatening.
Treatment of Endodontic Infections
The key to successful treatment of endodontic
infections is removal of the reservoir of infection
(necrotic tissue, bacteria, and bacterial by-products) by
the following procedures:
1. Debridement of the root canal system by mechanical
instrumentation
2. Root canal irrigation: NaOCl & EDTA
3. Intracanal medication: Ca(OH)2
4. Drainage
End of the Lecture

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