This document discusses endodontic microbiology and the treatment of endodontic infections. It covers the portals of entry for bacteria to enter the pulp, the role of bacteria in pulp necrosis and periapical disease, and some of the common bacterial species found in endodontic infections like Enterococcus faecalis. It also discusses abscesses, cellulitis, and the treatment of endodontic infections which involves debridement, irrigation with sodium hypochlorite and EDTA, intracanal medication with calcium hydroxide, and drainage.
This document discusses endodontic microbiology and the treatment of endodontic infections. It covers the portals of entry for bacteria to enter the pulp, the role of bacteria in pulp necrosis and periapical disease, and some of the common bacterial species found in endodontic infections like Enterococcus faecalis. It also discusses abscesses, cellulitis, and the treatment of endodontic infections which involves debridement, irrigation with sodium hypochlorite and EDTA, intracanal medication with calcium hydroxide, and drainage.
This document discusses endodontic microbiology and the treatment of endodontic infections. It covers the portals of entry for bacteria to enter the pulp, the role of bacteria in pulp necrosis and periapical disease, and some of the common bacterial species found in endodontic infections like Enterococcus faecalis. It also discusses abscesses, cellulitis, and the treatment of endodontic infections which involves debridement, irrigation with sodium hypochlorite and EDTA, intracanal medication with calcium hydroxide, and drainage.
This document discusses endodontic microbiology and the treatment of endodontic infections. It covers the portals of entry for bacteria to enter the pulp, the role of bacteria in pulp necrosis and periapical disease, and some of the common bacterial species found in endodontic infections like Enterococcus faecalis. It also discusses abscesses, cellulitis, and the treatment of endodontic infections which involves debridement, irrigation with sodium hypochlorite and EDTA, intracanal medication with calcium hydroxide, and drainage.
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ENDODONTIC
MICROBIOLOGY ENDO 412 Lecture No 5 part II
Dr. Ammar Abu Mostafa
Learning outcomes Portals of entry to the pulp Bacterial role in Pulp and Peri-apical pathosis Enterococcus faecalis Absecess and Cellulitis Management of the infected teeth Portals of Entry to the Pulp Caries Through anomalous tract like radicular lingual groove Through fracture line Via exposed dentin Around restorations From periodontal pockets to the pulp via apical foramen, accessory and lateral canals Pulp Necrosis & Bacterial Invasion Bacterial penetration into dentinal tubules of necrotic teeth is greater than teeth with vital pulp, due to the following reasons: 1. The presence of fluids in dentinal tubules 2. The presence of odontoblastic processes 3. Formation of tertiary dentin
Vital pulp is more resistant to bacterial invasion than
necrotic pulp Bacterial Role in Pulp Infection In an experimental study; after intentional pulp exposure, pulp necrosis and periradicular lesion developed in rats with normal microbial flora but not in germ-free rats Microorganisms invade the necrotic pulp and infect the root canal system, including the dentinal tubules
Coccal forms of bacteria seen in the
cross-section of a fractured dentinal tubule Most of the bacteria in an endodontic infection are strict anaerobes With increasing time and depth of pulp infection, the microbial flora changes from a predominantly facultative, Gram-positive flora to an almost completely anaerobic and mainly Gram-negative flora. Endodontic infections are polymicrobial, no single microorganism or group of bacteria has been proven to be more pathogenic than another. The following strains are commonly found in endodontic infections: Fusobacterium , Prevotella, Prophyromonas, Peptostreptococcus, and Veillonella. The microbial flora in retreatment cases is different from bacteria in primary apical periodontitis. Typically retreatment cases show enterococcus faecalis , streptococci, and anaerobic Gram-positive rods in high frequencies. Fungi have been identified in root canals and in teeth refractory to RCT. Viruses, including HIV, are also present in the pulp. Bacterial Role in Periradicular Disease Bacteria and bacterial by-products of the root canal play the major role of periradicular lesion The bacteria are polymicrobial and are similar to those isolated from infected root canals Resistance of Oral Microbes to Medicaments Enterococci has been shown to be relatively resistant to Ca(OH)2 and occur in high frequency in retreatment cases Abscess & Cellulitis Abscess is a cavity containing pus (purulent exudate) consisting of bacteria, bacterial by-products, inflammatory cells, numerous lysed cells, and the contents of those cells. Cellulitis is a diffuse, erythematous, mucosal, or cutaneous infection that may rapidly spread into deep facial spaces and become life threatening. Treatment of Endodontic Infections The key to successful treatment of endodontic infections is removal of the reservoir of infection (necrotic tissue, bacteria, and bacterial by-products) by the following procedures: 1. Debridement of the root canal system by mechanical instrumentation 2. Root canal irrigation: NaOCl & EDTA 3. Intracanal medication: Ca(OH)2 4. Drainage End of the Lecture