Abhay Katare NCSC
Abhay Katare NCSC
Abhay Katare NCSC
VINDHYANAGAR
NCSC project- Aristotle
NATIONAL CHILDREN’S SCIENCE
CONGRESS - 2017
(NCSC)
PROJECT PRESENTATION
INTRODUCTION
• Coal is the most abundant, extensively used,
and important source of energy for power
generation in the world.
• Among the total power generated annually in
India, about 70% is produced by thermal
power plants.
• About 112 million tons of fly ash is being
generated annually in India by thermal power
plants that will reach 170 million tons by the
year 2017
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The abundance of the coal produced form the thermal power projects
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So, the problem faced by the people was the disposal of
this generated fly ash form the power plants that where
to dispose this FA (fly ash)
The other use was that to use them in land fills which is
again not meeting the need rather there were conflicts
with the required need of the population that is land
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Abstract
• The research program consists of utilizing the
mineralogical properties of fly ash (collected from
NTPC, VINDHYANAGAR) such as porosity, low bulk
density, presence of inorganic metal ions, and its macro
nutrient property. Fly ash (pH: 6.5) with 200mesh size
was treated by a technique called Bioleaching for
extraction of low grade mineral concentrates from
industrials wastes like fly ash by conversion of an
insoluble metal into a soluble form using Thyobacillus
ferrooxidans a gram negative, rod shaped economic
bacterium in the field of leaching.
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INDUSTRIAL WASTE A BOON FOR
SUSTAINABLE AGRICULTURE
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INDUSTRIAL WASTE A BOON FOR
SUSTAINABLE AGRICULTURE
Fly ash addition to soil improves various physical,
chemical and biological properties of soil and thereby
is also beneficial for sunflower plant growth with
increased seed yield.
The bacteria Thyobacillus ferrooxidans which is
extracted from the sewage water is very useful to
convert insoluble minerals and extract the soluble
minerals from them which in turn is perfect for
agricultural growth and hence is really a BOON for
SUSTAINABLE AGRICULTURE which is todays
needs
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THIS IS THE INCREASE IN THE SIZE AND GROWTH
OF VARIOUS CROPS WITH FLY ASH ALONE
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• WHY ??? BECAUSE OF ITS HARMFUL
EFFECTS OF SULPHUR AND OTHER
INSOLUBLE MINERALS WHICH CAN HARM
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THE SOIL
Aims & Objectives
• 1. To prepare biofertilizer from fly ash generated in industry
• 2. To use the prepared biofertilizer for plant growth
• 3. To overcome the adverse effect of these problems
caused by the direct usage of the fly ash by bioleaching
hence improving its compatibility with soil
• • Fly-ash is regularly generated as a by-product by any
established coal or thermal power stations.
• • Fly-ash is one of the most challenging waste materials in
the world.
• • Fly-ash leads to degradation of arable land and
contaminate air, water, soil and organisms in the zone of
influence.
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Work Plan
• First the complete idea of the work plan was to
survey the site and collect some samples of the fly
ash and the sewage water for the desired test form
NTPC vindhyanchal and HINDALCO Bargawan
• Microbial analysis of sewage waste was done for the
desired bacteria thyobacillus ferrooxidants
• Isolation of bacteria by serial dilution method and
gram staining.
• BIOLEACHING OF FLY ASH via Thyobacillus
ferrooxidans
• Mixing of fly ash and soil together for biofertilizer
formulation
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Methodology - Survey based,
Experimental or both
EXPERIMENTAL STUDY
To achieve the objectives outlined, a proper study plan was made
for achieving the goals.
Thyobacillus ferrooxidans is the most important genus of
chemo-lithotrophs that metabolize sulfur and iron by
oxidizing it and producing sulphuric acid in the aqueous
phase. The remaining solids are discarded. Thiobacilli can
well adapt to wide variations of temperature and pH and
can be readily isolated and enriched. These are generally
isolated from industrial ponds, sewages, mining area,
drainage effluents, rivers, canals etc. (In our case it was
the sewage waste water of NTPC plant)
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BIOLEACHING OF FLY ASH via Thyobacillus
ferrooxidans
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1. Thyobacillus ferrooxidans a gram negative, rod
shaped bacteria due to its bio-hydrometallurgical
property acts as an economic bacterium in the
field of leaching.
2. This is collected from sewages then, followed by
serial dilution and multiplied by media
preparation which serves as nutrients for growth
of the bacterium thereby increasing the bacterial
counts. After this bioleaching is carried out for
extraction of unwanted metals present in the fly
ash (pH: 6.5) stock.
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EXPERIMENTS CARRIED OUT IN
THE BIOTECH LAB OF SCHOOL
• BIOLEACHING
• STERLIZATON OF THE APPARATUS
AND MEDIA (By UV rays and 70% ethanol)
• PROVIDING CONDITIONS FOR
GROWTH CHECKING
• MIXING OF THE CULTURED
BACTERIA WITH SOIL AND THUS A
BIO FERTILIZER
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BIOLEACHING
• Bioleaching is a process for extraction of
metals from their mineral source by oxidation
of metals by naturally occurring
microorganisms so as to extract certain
elements from the material on filtration via
water.
• Microbial technology renders helps in case of
recovery of ores which cannot be
economically processed with chemical
methods, because they contain low grade
metals.
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BIOLEACHING
• Bioleaching‟ which is one of the most used
treatment technique for extraction of low
grade mineral concentrates from mineral ore
(red mud) and industrials wastes like fly ash by
conversion of an insoluble metal (usually a
metal sulfide, e.g., FeS2) into a soluble form
(usually the metal sulfate, e.g., FeSO4) using
microbes for gainful and safe utilization on
sustainable basis.
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OBSERVATION
It was observed that though the fly ash
generated after complete burning from
same grade of coal (class-C), account to
variation in mesh size and moisture
content.
The mesh size of HINDALCO-coal is
coarser as compared to NTPC COAL,
which results in a little higher moisture
holding capacity of HINDALCO-coal than
NTPC COAL.
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OBSERVATIONS
On the contrary-wise more the mesh size of
fly ash, better would be its usage in relation
to biofertillizer for crop growth as it would
increase the water logging capacity.
Also more the moisture content in fly ash
better it is considered with more interaction
of the microorganisms increasing the bio-
oxidation process by Thyobacillus
ferrooxidans enhancing the leaching process.
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COMPARITIVE PLANT
SOWING NTPC
VINDHYANCHAL ASH
MIXED WITH SOIL
HINDALCO
NTPC TREATED
ASH
MIXEDWITH
SOIL
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PLANT 1
PLANT 2
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Data Analysis & Interpretation
VERIFIED IN LOG BOOK
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RESULTS
• From the data so noted above we observe a
good and faster growth in NTPC-Fly ash than
the HINDALCO-Fly ash. This concludes to
our experimentation that, NTPC-Fly ash is
SLIGHTLY better for plant growth than the
other one, though HINDALCO-Fly ash can
also serve the same purpose but with A BIT
less productivity.
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Conclusions
• Plant growth results proved that fly ash can be used
as a biofertillizer after proper treatment via.
bioleaching by the beneficiary effect of Thyobacillus
ferrooxidans and its metal extracting nature and
comparative study on this basis was also successful.
• TO OVERCOME THE HARMFUL EFFECTS OF FLY ASH
MENTIONED LATTER WE ARE USING BIOLEACHED
FLY ASH
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Solution to the Problem
– Application of fly ash also improves WHC
(reducing irrigation requirement by about 10-
15%), improves texture, aeration and reduces
crust formation.
– Fly ash is also a good amendment for acidic and
saline soils as well as for reclamation of other
degraded/problematic soils.
– One time field application of fly ash/pond has
shown sustained improvement in yield and soil
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quality 7-8 years 28
References
Xuan jing ke xue (2005), Bioleaching of fly ash from municipal
solid waste incinerator using sewage sludge and pig manure as
culture media.
S. Aggarwal, G.R. Singh and B.R. Yadav (2009) Utilization of Fly ash for Crop
Production: Effect on the Growth of Wheat and Sorghum Crops and Soil
Properties. Journal of Agricultural Physics, Vol. 9, pp
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THOUGH FLY ASH IS A WASTE OF
CONCERN BUT NOW HAS BECOME
A BOON FOR SUSTIANABLE
AGRICULTURE
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