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Plant Viruses

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PLANT VIRUSES

 Virus is a submicroscopic, transmissible,


intercellular, obligate parasite and consists of
nucleic acid (either RNA or DNA), which is
typically surrounded by a protein coat. They
are less than 200 milli micron and cannot be
grown in artificial media and require living
host cell for multiplication. They have both
living and nonliving properties.
Characteristics of viruses
 Characteristics of viruses which separate them
from other causes of plant pathogens are: They
are acellular.
 They are sub-microscopic and intracellular.
They lack lipid membrane system and energy
production.
 They use host machinery for their replication.
STURCTURE OF VIRUS

 Virion is a technical term used for the virus particle.A


virion consists of nucleic acid surrounded by a protein
coat.
 The nucleic acid is called _nucleoid' which may be either
de-oxyribonucleic acid (DNA) or ribonucleic acid RNA
(mostly RNA in plant viruses), but never both; and forms
the genome.
 The protein coat is called capsid'. It consists of many
subunits which are similar and occasionally dissimilar,
and these subunits are called capsomeres.
MORPHOLOGY OF VIRUSES
1. Rigid rod: (E.g.) Tobacco Mosaic Virus (TMV) and
Tobacco rattle Virus (TRV)
2. Flexuous rod: (E.g.) Potato Virus X (PVX), Bean
Common Mosaic Virus (BCMV).
3. Filamentous rod: (E.g.) Tenuiviruses likes Rice Grassy
Stunt (RGSV) and Rice Stripe Virus (RSV).
4. Isometric: (E.g.) Rice Tungro Spherical Virus (RTSV),
Cucumber Mosaic Virus (CMV), Tomato Spotted Wilt
Virus (TSWV).
5. Bacilliform: (E.g.) Rice Tungro Bacilliform Virus
(RTBV), Banana streak virus (BSV) and Cocoa Swollen
Shoot Virus (CCSV).
MULTIPLICATION OF
VIRUS
Multiplication of virus is different from fungi and bacteria.
First step in the multiplication is the separation of nucleic

acid from the protein coat in the host cell by the enzymes of
host cell.
Nucleic acid itself involve in the synthesis of new nucleic

acid and protein coat by utilizing the amino acids, ribosome


and transfer RNA of the host.
Once the new nucleic acids and proteins subunits are

formed, the nucleic acid arranges the protein subunit


around it to form the complete virus particle or virons.
 Vector is an organism that carries and
transmits a pathogen (inoculum) to a plant.
Vectors may be insect, nematodes, fungi, etc.
 Symptoms like chlorosis, mosaic, streak, vein
clearing, vein banding, leaf crinkle, leaf curl,
enation, necrosis, dwarfing, rosette, bunchy
top, twisting etc. are produced in crop plants.
VIROIDS
Viroids area covalently closed circular RNA molecules.
Viroids were the first circular RNAs to be discovered in
nature.
These are the smallest known infectious agents. Potato
spindle tuber viroid was the first viroid reported, and
it is widely prevalent in different potato growing areas.
Citrus exocortis viroid is wide spread in citrus
production areas where trifoliate ornage(Poncirus
trifoliate)is used as root stock.
VIRUSOIDS (ENCAPSIDATED,
VIROIDLIKE, SATELLITE RNA'S)
Some
 viruses contain a viroid like satellite RNA in addition to a linear, single
stranded molecule of genomic RNA.
Such viroid -like satellite RNA's are called virusoids.

They show little sequence homology with viroids, but they do show significant

homology with the linear satellite RNA associated with Tobacco ringspot virus.
The virusoids in infefcted plants exist almost solely as circular molecules, either

free or encapsidated within virion of the helper virus.

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